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Diagnosis of Trypanosoma evansi by the polymerase chain reaction (PCR) OAK
Donelson, J. E.; Artama, W. T..
Palavras-chave: Trypanosoma evansi; Diagnosis; Polymerase chain reaction.
Ano: 1998 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/300
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DIAGNÓSTICO DA AMBIÊNCIA ORGANIZACIONAL DA COMUNIDADE DE PRODUTORES DE PIMENTA LONGA (PIPER HISPIDINERVUM) UNIÃO DA LINHA SEIS DO PROJETO DE COLONIZAÇÃO HUMAITÁ EM PORTO ACRE – AC AgEcon
Teixeira, Solange Maria Chalub Bandeira; Carvalho, Lucas Araujo.
Este trabalho apresenta o resultado parcial de uma pesquisa maior sobre a avaliação ambiental e sócio-econômica da produção de pimenta longa (Piper hispidinervum) de uma comunidade de produtores de um projeto de colonização do INCRA. Trata-se da sistematização do que foi levantado em campo, através de pesquisa realizada com 10 (dez) produtores rurais, pertencentes a uma associação de produtores do Projeto de Colonização Humaitá (INCRA), no município de Porto Acre (Ac). O diagnóstico contemplou o levantamento de forças propulsoras e restritivas inerentes à ambiência de cada propriedade rural, elencadas numa matriz. Os resultados mostraram a necessidade de rever as políticas públicas e a gestão dos negócios rurais, de forma a dar subsídios e mais respaldo às...
Tipo: Conference Paper or Presentation Palavras-chave: Avaliação ambiental e sócio-econômica; Pimenta longa; Comunidade de produtores rurais; Diagnóstico; Desenvolvimento sustentável; Políticas públicas; Environmental and socio-economical evaluation; Long pepper; Rural producers community; Diagnosis; Supporting development; Public politics; Crop Production/Industries.
Ano: 2008 URL: http://purl.umn.edu/109231
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DIAGNÓSTICO DE LA CADENA OVINA EN LA PROVINCIA DE CÓRDOBA, ARGENTINA AgEcon
Aguero, Daniel; Freire, Viviana; Peralta, Marcela; Vigliocco, Mauricio; Sandoval, Gabriela.
The continuous process toward agriculture is the main reason of the substitution and even disappearance of the ovine cattle of the province. The internal market is barely developed and strongly seasonal. The objective of this work is to characterize the chain of ovine meat of the province of Cordoba. The used data come from primary information: surveys and interviews to described informants, and of secondary information. Even though, the ovine production net has a complementary character of other cattle activities and agricultural, the present and potential demand of the lamb meat follows in increase. This study also has detected important restrictions for the integral development of the chain, the most notable aim to the tax burden, the sanitary problems...
Tipo: Journal Article Palavras-chave: Diagnosis; Ovine meat chain; Cordoba-Argentina; FODA.; Agribusiness.
Ano: 2010 URL: http://purl.umn.edu/56557
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Identification of turkey astrovirus and turkey coronavirus in an outbreak of Poult Enteritis and Mortality Syndrome Rev. Bras. Ciênc. Avic.
Villarreal,LYB; Assayag,MS; Brandão,PE; Chacón,JLV; Bunger,AND; Astolfi-Ferreira,CS; Gomes,CR; Jones,RC; Ferreira,AJP.
This article reports a survey on turkey astrovirus (TAstV) and turkey coronavirus (TCoV) infections with RT-PCR in 17 turkey flocks affected by acute enteritis and two apparently normal turkey flocks located in the Southeastern region of Brazil by PCR (TAstV and TCoV). Seven out of the 17 affected flocks were positive for TAstV and 14 for TCoV, with seven co-infections. In one of the two apparently normal flocks, a TAstV-TCoV co-infection was found. Although a definitive association of these agents and the signs can not be made, the implications of these findings are discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Astrovirus; Coronavirus; Turkey; Enteritis; Diagnosis.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000200010
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Diagnosis of infectious bronchitis: an overview of concepts and tools Rev. Bras. Ciênc. Avic.
Villarreal,LYB.
Infectious bronchitis (IB) casues multi-systemic infection in chickens with signs similar caused by other poultry pathogens and thus a high diagnostic accuracy can only be achieved by s series of laboratory assays. This article reviews in a brief way the traditional virus assays such as embryo innoculation, tracheal rings and virus neutralization assays for the direct detection of Avian infectious bronchitis virus (IBV) and methods based on gene molecular biology and some assays for the detection of anti-IBV antibodies, including ELISA. A critical view on each technique is also provived by the author.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibodies; Diagnosis; IBV; Typing.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200006
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Enteric viruses in turkey flocks: a historic review Rev. Bras. Ciênc. Avic.
Alavarez,JM; Ferreira,CSA; Ferreira,AJP.
In this review, diagnostic techniques and viral agents involved in enteric diseases affecting turkeys are described. Data from field observations and laboratory researches have been reported in turkey flocks for over 70 years, and several viruses have been identified. After a period of 30 years of inoculation experiments and neutralization studies, adequate visualization of the viruses was achieved using electronic microscopy. During the following years, several studies were then conducted to isolate and classify those viruses using cell-culture, embryo-propagation, serological tests, genome electropherotyping by polyacrylamide gel electrophoresis of double-stranded RNA viruses, and recently, nucleic acid studies. Thus, since the 1990s, the nucleic-acid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Turkey; Enteritis; Viruses; Poults; Diagnosis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300001
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Litter characteristics and pododermatitis incidence in broilers fed a sorghum-based diet Rev. Bras. Ciênc. Avic.
Carvalho,CMC; Litz,FH; Fernandes,EA; Silveira,MM; Martins,JM da S; Fonseca,LA; Zanardo,JA.
The aim of this study was to evaluate the incidence of footpad dermatitis and quality of broilers litter fed with sorghum grain and diets based in corn. It was used 544 male and female chicks, distributed in a completely randomized design with two treatments and eight replications. The chicken feed was formulated and produced from corn, soybean meal and sorghum grain. In the formulation were kept constant levels of energy and protein in accordance with the following treatments: A. Control (diet based on corn and soybean meal); B. Grain sorghum (Whole Sorghum + soybean meal). At 35 and 42 days were evaluated mineral matter (A %), calcium (Ca%), phosphorus (P%), pH and dry matter (DM %) of the poultry litter. To evaluate the footpad dermatitis were evaluated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Turkey; Enteritis; Viruses; Poults; Diagnosis; Calcium; Environmental impact; Phosphorus.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300010
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Assessment of Broiler Chicken Welfare in Southern Brazil Rev. Bras. Ciênc. Avic.
Federici,JF; Vanderhasselt,R; Sans,ECO; Tuyttens,FAM; Souza,APO; Molento,CFM.
Abstract Scientific literature on broiler chicken welfare in Brazilian industrial systems is scarce. This study aimed at assessing broiler chicken welfare on industrial farms in the State of Rio Grande do Sul, Southern Brazil, using the Welfare Quality(r) assessment protocol for poultry, to provide directly applicable scientific information. Results are presented as criteria scores ranging from 0 to 100, with higher scores indicating better welfare; and percentages of prevalence. The scores classified as excellent (above 80) were absence of prolonged thirst, absence of prolonged hunger, litter quality, breast blister and touch test. Enhanced scores (between 55 and 80) were comfort around resting, plumage cleanliness and dust sheet test. Acceptable scores...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal welfare; Assessment; Diagnosis; Poultry welfare; Welfare Quality(r).
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100133
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Susceptibility of mammalian cell line for isolation of IBDV from clinical samples Rev. Bras. Ciênc. Avic.
Silva,SEL; Ferreira,HL; Carvalho,BA; Cardoso,TC.
Susceptibility of chicken embryo related cells (CER) to non-classical IBDV was compared with Vero cells for the same purpose. It was used 20 bursal samples collected from clinical cases of IBDV, positive by histopathological evaluation. After three times infection of CER and Vero cells monolayers, at 6, 12, 18, 24, 48, 60 and 72h post-infection (PI), extra-cellular and intracelluar IBDV was measured. The extra-cellular amount of IBDV particles was quantified by DAS-ELISA from 6h PI, and increased for both cells studied until 72h PI. The IBDV re-isolation performed by infecting chicken embryos showed positive results at all PI studied. The intracellular IBDV RNA was shown by RT-PCR, where all tested samples were positive. However, the cytopathic effect...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Avibirnavirus; CER cells; Diagnosis; Double antibody sandwich ELISA; RT/PCR.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000100009
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Identification of Eimeria mitis and Eimeria praecox in broiler feces using polymerase chain reaction Rev. Bras. Ciênc. Avic.
Meireles,MV; Roberto,LO; Riera,RF.
There are few reports concerning the epidemiology of Eimeria praecox and Eimeria mitis in Brazil. In the present experiment, the polymerase chain reaction (PCR) was used to identify these species in 156 samples of broiler chicken feces from several Brazilian states and the Federal District. Oocysts present in feces samples were purified by sodium chloride flotation followed by addition of DNAzol reagent (Invitrogen®) for extraction of genomic DNA. DNA was precipitated and stored following DNAzol reagent manufacture's instructions. The primers and PCR conditions were as described by Schnitzler et al. (1999). In the 156 field samples analyzed by PCR, 70 and 45 were positive for E. praecox and E. mitis, respectively. In this study we have shown that DNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diagnosis; Eimeria mitis; Eimeria praecox; Epidemiology; Polymerase chain reaction.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000400010
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Avian mycoplasmosis update Rev. Bras. Ciênc. Avic.
Nascimento,ER; Pereira,VLA; Nascimento,MGF; Barreto,ML.
Avian mycoplasmas occur in a variety of bird species. The most important mycoplasmas for chickens and turkeys are Mycoplasma gallisepticum (MG), M. synoviae (MS), and M. meleagridis. Besides, M. iowe (MI) is an emerging pathogen in turkeys, but of little concern for chickens. Mycoplasmas are bacteria that lack cell wall and belong to the class Mollicutes. Although they have been considered extracellular agents, scientists admit nowadays that some of them are obligatory intracellular microorganisms, whereas all other mycoplasmas are considered facultative intracellular organisms. Their pathogenic mechanism for disease include adherence to host target cells, mediation of apoptosis, innocent bystander damage to host cell due to intimate membrane contact,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Airsacculitis; Diagnosis; Mycoplasma; Mollicutes; Poultry.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2005000100001
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Evolution and perspective of the temperate fruit crops in São Paulo state, Brazil Rev. Bras. Frutic.
Tecchio,Marco Antonio; Bettiol Neto,José Emilio; Barbosa,Wilson; Tucci,Maria Luiza Sant'ana.
This work was carried out to show the current situation of the temperate fruit crops in São Paulo state, Brazil, with an emphasis on grapes, peaches, apples, plums, nectarines and pears crops. Current economic data of crops, major growing regions, main cultivars produced, as well as the new technologies generated by research are presented. Regarding the grape crop, a decrease in the national production as well as in the major growing states was observed. The main grapes growing centers in São Paulo state are presented, highlighting its peculiarities regarding cultivars, cultural crop management and current researches. A trend has been observed toward increasing Niagara Rosada grape growing area rather than the fine table grape cultivars. It was also...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Temperate fruit crops; Economics; Diagnosis; Cultural management.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452011000500018
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Stephanofilariasis and nodular ulcerative dermatitis in dog: case report MV&Z
Novaes, Antonio Pereira de.
Objective: To report on the occurrence of stephanofilariasis in a dog, causing nodular ulcerative dermatitis in the abdominal area. Description: Dog patient, mixed breed, approximately 10 years old, presenting with nodular ulcerative dermatitis in the abdominal area. Suspected etiology was Stephanofilaria spp. AfieI' surgical excision of the lesion, anim print was performed, followed by fixation with ethanol and staining with 1:20 Giensa and saturated Congo resolution. The parasites (larval and adult forms) were identified under optical microscope (200 x). Results: The method employed allowed for visualization of the parasite, thus confirming the occurrence of stephanofilariasis in dogs. Conclusions: [t was demonstrated that Stephanofilaria spp can also...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Filariasis; Diagnosis; Dermatitis; Skin ulcer; Drug therapy; Lvermectin; Dogs Filariosis; Diagnóstico; Dermatitis; Úlcera cutánea; Quimioterapia; Lvermectina; Perros filariose; Diagnóstico; Dermatite; Úlcera cutânea; Quimioterapia; Ivermectina; Cães.
Ano: 2005 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3129
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Antebrachiocarpal joint synovial sarcoma in a dog MV&Z
Fonseca Pinto, Ana Carolina Brandão de Campos; Tatarunas, Angélica Cecilia; Chalita, Maria Carolina Catai; Michalany, Nilceo Schwery; Matera, Julia Maria.
Objective: To describe the clinical signs and radiographic, cytological and histopathological findings in a dog presented with antebrachiocarpal joint synovial sarcoma. The literature search was performed in CAB and MEDLINE databases, for the retrospective period of-t8 years. Description: A 9 year-old mongrel intact male dog was presented t0 the Small Animal Surgery Service of the Veterinary Hospital of the School of Veterinary Medicine and Animal Science of the University of São Paulo, São Paulo, SP, Brazil, for evaluation of a subcutaneous soft issue mass located cranial to the right antebrachiocarpal joint. Radiographic and cytologicalfindings suggested a malignant neoplasm, which was confirmed by the histopathological analysis to be a synovial sarcoma....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neoplasms; Sarcoma; Synovial; Diagnosis; Arthrography; Joints; Surgery; Amputation; Dogs Neoplasmas; Sarcoma sinovial; Diagnóstico; Artrografia; Articulaciones; Cirurgía; Amputación; Perros neoplasias; Sarcoma sinovial; Diagnóstico; Artrografia; Articulações; Cirurgia; Amputação; Cães.
Ano: 2004 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3235
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Severe phlegmonous cystitis: case report MV&Z
Nakamuta, Bruna Higa; Kolber, Milton; Nagasse, Nathalie.
A case of severe phlegmonous cystitis or purulent cystitis due to bacterial infection is reported in a 9-year-old female dog of the Dachshund breed, presenting dysuria about a week ago. Biochemical examinations were performed, besides calculation analysis and abdominal ultrasound examination. The biochemistry exams did not present alterations. Calculation analysis showed calcium carbonate/urate. The diagnosis was confirmed by ultrasonography in which a heterogeneous, hypoechogenic and hyperechogenic aspect was seen in the caudal region of the bladder. The animal underwent surgery, in which a mass adhered to the bladder was removed and sent for histological examination, resulting in loss of epithelial lining and severe leukocyte infiltration. This report...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cystitis; Diagnosis; Dog; Infection; Severe; Ultrasonography; Cão; Cistite; Diagnóstico; Infecção; Severa; Ultrassonografia.
Ano: 2019 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/37916
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Redescripción de la obrera de Myrmelachista gallicola (Hymenoptera: Formicidae) y primera cita para la provincia de La Pampa (Argentina) Rev. Soc. Entomol. Argent.
Quirán,Estela M.; Martínez,Juan J..
Se redescribe la obrera de Myrmelachista gallicola Mayr y se cita por primera vez para la provincia de La Pampa (Argentina). El material estudiado está compuesto por obreras y fue recolectado sobre Prosopis caldenia Burkart (Fabaceae), dentro de las agallas de Tetradiplosis sp. (Diptera: Cecidomyiidae), en la periferia de la ciudad de Santa Rosa, provincia de La Pampa.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Myrmelachista gallicola; Diagnosis; Distribución; Biología; Prosopis caldenia; Cecidomyiidae.
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0373-56802006000200014
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First record of the genus Kybos (Auchenorrhyncha: Cicadellidae: Typhlocybinae) for Argentina Rev. Soc. Entomol. Argent.
Catalano,María Inés; Paradell,Susana L.; Remes Lenicov,Ana M. de.
The genus Kybos Fieber and the species K populi (Edwards) are recorded for the first time for the Neotropical region from specimens collected in Mendoza province, Argentina. This contribution adds taxonomic and bioecologic knowledge about this genus and its species.
Tipo: Info:eu-repo/semantics/report Palavras-chave: First record; Diagnosis; Distribution; Host plant.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0373-56802012000100020
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Molecular characterization of GSyV-1 and GLRaV-3 and prevalence of grapevine viruses in a grape-growing area Scientia Agricola
Moura,Cátia Jacira Martins de; Fajardo,Thor Vinícius Martins; Eiras,Marcelo; Silva,Fábio Nascimento da; Nickel,Osmar.
ABSTRACT: The aims of this study were to determine the prevalence of viruses in 119 samples from 32 grapevine cultivars, collected from nine vineyards in a specific grape-growing area in southeastern Brazil, perform a partial molecular characterization of 14 isolates of Grapevine Syrah virus 1 (GSyV-1) and Grapevine leafroll-associated virus 3 (GLRaV-3) and assess the coat protein genetic variability of these viruses. The detection of viruses was implemented by real-time RT-PCR (reverse transcription polymerase chain reaction) aiming to detect seven viruses and one viroid. With the exception of the Grapevine Cabernet Sauvignon reovirus (GCSV), the viruses and viroid that were evaluated were widespread in the sampled areas, often in high prevalence and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vitis; Diagnosis; Variability; Incidence; Leafroll.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000100043
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Detection of Rhynchosporium secalis in barley seeds from Argentina through polymerase chain reaction technique Trop. Plant Pathol.
Ríos,Melina Ocampo; Fernández,Paula; Carmona,Marcelo.
Leaf scald of barley caused by Rhynchosporium secalis is an important disease in Argentina. The fungus is a necrotrophic pathogen which survives in stubble, seeds and weeds. Isolation of R. secalis from seeds on artificial media usually has not been successful due to the slow growth rate of the pathogen and strong inhibition by contaminants. The objective in this work was to detect R. secalis in different genotypes of barley seeds in Argentina using the polymerase chain reaction (PCR)-based diagnostic assay. Four barley genotypes were tested in 2004: Quilmes Ayelén, Quilmes Alfa, Barke and Maltería Pampa 1004. The previously described RS8 and RS9 primers were used for the detection of R. secalis in barley seeds. A 264-bp single band was obtained for each...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hordeum vulgare; Diagnosis; PCR.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000500007
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RT-PCR and Dot Blot hybridization methods for a universal detection of tospoviruses Trop. Plant Pathol.
EIRAS,MARCELO; RESENDE,RENATO O.; MISSIAGGIA,ALEXANDRE A.; ÁVILA,ANTÔNIO C. DE.
Transcriptase reverse - polymerase chain reaction (RT-PCR) and dot blot hybridization with digoxigenin-labeled probes were applied for the universal detection of Tospovirus species. The virus species tested were Tomato spotted wilt virus, Tomato chlorotic spot virus, Groundnut ringspot virus, Chrysanthemum stem necrosis virus, Impatiens necrotic spot virus, Zucchini lethal chlorosis virus, Iris yellow spot virus. Primers for PCR amplification were designed to match conserved regions of the tospovirus genome. RT-PCR using distinct primer combinations was unable to simultaneously amplify all tospovirus species and consistently failed to detect ZLCV and IYSV in total RNA extracts. However, all tospovirus species were detected by RT-PCR when viral RNA was used...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diagnosis; Plant viruses; Bunyaviridae.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000200009
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