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| Registros recuperados: 89 | |
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| Salvadori,Márcia Regina; Valadares,Geórgio Freesz; Leite,Domingos da Silva; Blanco,Jesús; Yano,Tomomasa. |
| Two hundred and five Escherichia coli strains isolated from calves with diarrhea from mid-western Brazil were screened for the presence of virulence factors associated with bovine colibacillosis. One hundred and two (49.8%) of the E. coli strains produced toxins: Shiga toxins 1 (9.7%) and 2 (6.3%), a-hemolysin (9.7%), enterohemolysin(6.8%), Cytotoxic Necrotizing Factors type 1 (0.5%), and type 2 (4.4%), enterotoxins LT-II (8.3%) and STa (3.9%). No strain produced enterotoxin LT-I. Fimbrial adhesins F5 and F17 were produced by 7.3% and 4.8% of the strains, respectivly, and none expressed F41. Seven strains (3.4%) possessed the gene eae and belonged to serotypes O26:H-; O111:H- and O118:H16. These results suggest that calves in Brazil may be an important... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calves; Diarrhea; E. coli; Virulence factors. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300009 |
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| Fernandes,M.C.; Takai,S.; Leite,D.S.; Pinto,J.P.A.N.; Brandão,P.E.; Santarém,V.A.; Listoni,F.J.P.; Silva,A.V. Da; Ribeiro,M.G.. |
| The identification of pathogens of viral (Rotavirus, Coronavirus), parasitic (Toxocara spp.) and bacterial (Escherichia coli, Salmonella spp., Rhodococcus equi) origin shed in feces, and the virulence profile of R. equi and E. coli isolates were investigated in 200 samples of sand obtained from 40 parks, located in central region of state of Sao Paulo, Brazil, using different diagnostic methods. From 200 samples analyzed, 23 (11.5%) strains of R. equi were isolated. None of the R. equi isolates showed a virulent (vapA gene) or intermediately virulent (vapB gene) profiles. Sixty-three (31.5%) strains of E. coli were identified. The following genes encoding virulence factors were identified in E. coli: eae, bfp, saa, iucD, papGI, sfa and hly. Phylogenetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enteric pathogens; Virulence; Sand; Feces; Parks; E. coli; R. equi. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200025 |
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| Pourakbari,Babak; Ferdosian,Farzad; Mahmoudi,Shima; Teymuri,Mostafa; Sabouni,Farah; Heydari,Hossein; Ashtiani,Mohammad Taghi Haghi; Mamishi,Setareh. |
| Emerging antimicrobial resistance rates and Extended-spectrum beta-lactamase producing Escherichia coli recovered from urinary tract infections (UTI) is an increasing problem in specific regions, limiting therapeutic options. One hundred E. coli isolates causing UTI in patients with age from 2 months to 12 years admitted at CMC in the period of April 2009 to March 2010 were tested for antibiotic susceptibility using the disk diffusion method. Surprisingly high resistance rates were recorded for E. coli against TMP/SMX (84%), cefalotin (66%), cefuroxime (50%), cefixime (50%) and ceftriaxone (45%). Antimicrobial susceptibility of E. coli isolates was followed by meropenem (98%), amikacin (95%), nitrofurantoin (91%) and gentamicin (68%). Extended spectrum... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: E. coli; Urinary Tract Infection; Antimicrobial susceptibility; ESBL. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200041 |
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| Huasai,Simujide; Chen,Aorigele; Wang,Chun-jie; Li,Yu; Tongrige,Bai. |
| Virulence genes of Escherichia coli (E. coli) isolates from healthy dairy cows were identified and characterized by a multiplex PCR assay and serogrouping test. The results showed that among the target genes, eaeA was most frequently detected, accounting for 22.11% (67/303) in all strains from 101 cows. For categorization of E. coli, aEPEC was the category with widest distribution detected in 55 (18.15%) strains from 22 cattle. All of 84 PCR-positive strains belonged to 14 O serogroups, and O149 (25.00%) was most common identified, followed by O2 (17.86%), O8 (11.90%) and O103 (9.52%) with relatively high prevalence. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: E. coli; Virulence gene; Multiplex PCR; Serogroup; Healthy dairy cow. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200013 |
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| Menezes,Caroline Anunciação; Gonçalves,Danielle Silva; Amianti,Jackeline; Fernandes,Irene; Taddei,Carla Romano; Koga,Paula Célia Mariko; Trabulsi,Luiz Rachid; Martinez,Marina Baquerizo; Piazza,Roxane Maria Fontes. |
| A capture enzyme-linked immunosorbent assay (ELISA), which detects LT-I toxin produced by enterotoxigenic Escherichia coli strains, has beendeveloped. This capture assay was performed using the IgG enriched fraction of anti-LT-I antiserum and IgG2b anti-LT-I monoclonal antibody and allowed a clear distinction between E. coli LT-I - producing and non-producing strains. The estimated accuracy of the assay is 78% for sensitivity, 94% for specificity and 92% for efficiency. Thus, the capture immunoassayis a sensitive tool for detection of E. coli, which produces heat-labile enterotoxin, and is suitable for use in clinical laboratories and epidemiological surveys in developing world. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Detection; Polyclonal antibody; Monoclonal antibody; Thermo-labile toxin; E. coli. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500004 |
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| Roy,René; Shiao,Tze Chieh; Rittenhouse-Olson,Kate. |
| Combining nanotechnology with glycobiology has triggered an exponential growth of research activities in the design of novel functional bionanomaterials (glyconanotechnology). More specifically, recent synthetic advances towards the tailored and versatile design of glycosylated nanoparticles namely glyconanoparticles, considered as synthetic mimetics of natural glycoconjugates, paved the way toward diverse biomedical applications. The accessibility of a wide variety of these structured nanosystems, in terms of shapes, sizes, and organized around stable nanoparticles have readily contributed to their development and applications in nanomedicine. In this context, glycosylated gold-nanoparticles (GNPs), glycosylated quantum dots (QDs), fullerenes, single-wall... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dendrimer; Glycodendrimer; Vaccine; Bacteria; Nanoparticles; E. coli; Lanthanides. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000700008 |
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| Registros recuperados: 89 | |
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