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	Provedor de dados Infoteca-e (2) Autor CARVALHO, M. P. M. (2) LEAL, N. R. (2) VASCONCELLOS, H. O. (2) ALMEIDA, D. L. (1) ARAUJO, M. L. (1) FERNANDES, V. L. N. (1) GUIMARAES, I. S. S. (1) Mais... Palavra-chave Antocianin (1) Antocianinas (1) Análise Química (1) Batata Doce. (1) Chemical analysis. (1) Purple cabbage (1) Repolho roxo (1) Sweet potato. (1) Mais... Tipo do documento Comunicado Técnico (INFOTECA-E) (1) Outras publicações técnicas (INFOTECA-E) (1) Ano 1986 (1) 1983 (1) País Brazil (2) Idioma Português (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 113 Primeira ... 1 2 3 4 5 6 ... Última Micropropagation of Ilex dumosa (Aquifoliaceae) from nodal segments in a tissue culture system Biocell Luna,C.; Sansberro,P.; Mroginski,L.; Tarragó,J.. Micropropagation of Ilex dumosa var. dumosa R. ("yerba señorita") from nodal segments containing one axillary bud was investigated. Shoot regeneration from explants of six-year-old plants was readily achieved in ¹/4 strength Murashige and Skoog medium (¹/4 MS) plus 30 gr·L-1 sucrose and supplemented with 4.4 mM BA. Further multiplication and elongation of the regenerated shoots were obtained by subculture in a fresh medium of similar composition with 1.5 gr·L-1 sucrose. Rooting induction from shoots were achieved in two steps: 1) 7 days in ¹/4 MS (30 gr·L-1 sucrose, 0.25 % Phytagel®) with 7.3 mM IBA and 2) 21 days in the same medium without IBA and 20 mM of cadaverine added. Regenerated plants were successfully transferred to soil. This micropropagation... Tipo: Info:eu-repo/semantics/article Palavras-chave: Micropropagation; Nodal Segment; Cytokinins; Ilex Dumosa. Ano: 2003 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000200006 Behavior and preservation of an in vitro collection of European aspen in Spain Biocell Martin,M.T.; Pedranzani,H.E.; Sierra de Grado,R.. An in vitro collection has been established with selected European aspen from Palencia province (Spain). Currently, this collection includes 32 high quality clones, selected for their good bearing and healthy state. Most of them belong to different discrete local populations. Populus tremula L. was propagated in proliferation Aspen Culture Medium; they required subculture every 3 months. The purpose of this study was, therefore, to select a medium which allows the maintenance of 32 clones for a period longer than 3 months without subculture and to observe the behavior of those clones in 15 different culture medium compositions. Seven nodal cutting stem explants from each clone were cultured in parallel in the different media. One and three months after... Tipo: Info:eu-repo/semantics/article Palavras-chave: Populus tremula; Germplasm conservation; In vitro plant culture; Micropropagation; Selected clone. Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100005 In vitro spore culture and reproductive aspects of the annual fern Anogramma chaerophylla (Pteridaceae) Boletín de la Sociedad Luna,María L; Yañez,Agustina; Ramos Giacosa,Juan Pablo; Gorrer,Daniel; Berrueta,Pedro C; Giudice,Gabriela E. Anogramma chaerophylla belongs to a fern genus with annual sporophytes and potentially perennating gametophytes. In the studied area, Natural Reserve Punta Lara, the plants grow stressed mainly by pollution and the invasion of exotic species. As a part of a project on conservation of ferns inhabiting Buenos Aires province, the objectives of this work were to evaluate in Anogramma chaerophylla the conditions for in-vitro spore germination and to analyse the different stages of its reproductive cycle. Spores were sterilized in an aqueous solution 10 % of NaCIO during different times and then sown in Petri dishes with Murashige & Skoog medium, without the addition of sucrose. The dishes were kept under laboratory conditions, at 12 h light/ darkness... Tipo: Info:eu-repo/semantics/article Palavras-chave: Anogramma chaerophylla; Micropropagation; Gametophyte; Tubercle; Ex-situ conservation. Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722016000400010 Effect of 6-BA on nodal explant bud sproutings of Coffea arabica cv. Mundo Novo Bragantia Ramos,Luis Carlos da Silva; Almeida,Julieta Andrea Silva de. Coffee plants can be micropropagated by nodal bud sprouting using the 6-benzylaminopurine (6-BA) hormone. However, literature reports the use of a wide range of 6-BA, from 0.5 to 88.8 µM L-1. So, this study was performed to narrow that range. Nodal explants of Coffea arabica cv Mundo Novo obtained from in vitro plantlets were inoculated on gelled-MS medium supplemented with different concentrations of 6-BA. Two assays were carried out: in the first one, 6-BA was used at concentrations of 0, 5, 25, 50, and 100 µM L-1, being evaluated at 43 and 123 days. In the second experiment, dosis of 10, 20 and 30 µM L-1, have evaluated at 65 and 100 days. Treatments with 6-BA induced multiple sprouting from the nodal explants, which were best characterized around 100... Tipo: Info:eu-repo/semantics/report Palavras-chave: Coffee; Nodal bud; Micropropagation. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052005000200003 Production of pre-basic potato seed by polyvinyl chloride PVC: articulate gutters hydroponic system BABT Scherwinski-Pereira,Jonny Everson; Medeiros,Carlos Alberto Barbosa; Fortes,Gerson Renan de Luces; Pereira,Arione da Silva. The development of more efficient and productive systems for pre-basic seed potato production would improve the quality of the propagative material used by the potato growers, directly affecting the crop yields. A two-year experiment was carried out to evaluate the potato pre-basic seed production by two types of hydroponic systems (fibrocement tiles and articulated PVC gutters), two cultivars (`Baronesa` and `Eliza`) and two types of propagative material (plants coming from in vitro culture and minitubers). The PVC gutters system was highly efficient. When using minitubers, this system reached multiplication rates up to 74 tubers per plant. Minitubers were more productive than in vitro plants, independent of cultivar and hydroponic system utilized. Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum tuberosum; Soilless culture; Multiplication; Propagation; Minitubers; Micropropagation. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500007 In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe BABT Delmail,David; Labrousse,Pascal; Lesouëf,Jean-Yves; Le Hir,François; Autret,Jean-Luc; Botineau,Michel. Normania triphylla is an endemic species from Madeira island (Portugal) extinct in the wild since 1991. The aim of this work was to culture the meristems of this species in vitro and to multiply its shoots in order to preserve this endangered species. The best results in terms of multiplication were obtained in Murashige and Skoog medium supplemented with 10 µM 6‑benzylaminopurine (BAP). The number of shoots, the number of nodes and the number of leaves were the most important in this medium. However, the best results concerning the total shoot length were obtained when BAP was not supplemented into the medium and in the presence of 5 or 7.5 µM 1-naphtalene acetic acid (NAA). This is the first report on the in vitro culture of N. triphylla which could... Tipo: Info:eu-repo/semantics/article Palavras-chave: Normania; Endemic species; Micropropagation; 6‑benzylaminopurine; Callus. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400009 Induction of leafy galls in Acacia mearnsii De Wild seedlings infected by Rhodococcus fascians BABT Quoirin,Marguerite; Bona,Cleusa; Souza,Elizandra F. de; Schwartsburd,Pedro B.. Plantlets of blackwattle (Acacia mearnsii De Wild) were inoculated with the bacterium Rhodococcus fascians and cultured in vitro. Leafy galls appeared at the cotyledonary nodes in 75% of the infected plants. The galls were separated from the plants and cultured on a medium containing three-quarters-strength MS salts (Murashige and Skoog, 1962), MS vitamins, 2% sucrose and an antibiotic (cephalothin), supplemented with or without 0.2% activated charcoal. Histological studies conducted from the sixth to the twenty-second day after plant infection revealed the presence of newly formed meristematic centers, first in the axillary region, then on the petioles and lamina of the leaflets around the apical meristem. Approximately 37% of the galls developed one... Tipo: Info:eu-repo/semantics/article Palavras-chave: Black wattle; Legume tree; Micropropagation. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300002 Plant regeneration through somatic embryogenesis of yacón [smallanthus sonchifolius (poepp. and endl.) H. Robinson] BABT Corrêa,Cynthia Manyra; Oliveira,Graciele Nicolodi de; Astarita,Leandro Vieira; Santarém,Eliane Romanato. Smallanthus sonchifolius has tuberous roots containing large amounts of fructo-oligosaccharides and its medicinal use has increased due to the hypoglycemic properties reported for this species. An efficient system for propagation via somatic embryogenesis is reported using petiole segments cultivated on MS medium supplemented with combinations of BA, kinetin and 2,4-D, under light and darkness conditions. Embryogenic callus was formed in most of the treatments; however, somatic embryogenesis was promoted by the presence of light. Clusters of somatic embryos appeared on callus surface after 50 days of culture. The highest number of embryos was produced on 0.45 µM BA and 4.5 µM 2,4-D. Embryogenic calli were maintained on MS medium containing 4.5 µM BA and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plant; Tissue culture; Polymnia sonchifolia; Micropropagation. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300005 In vitro multiplication of 'Flordaguard' rootstock: cytokinin source and concentration effects, explants orientation and period of permanence in the culture medium BABT Radmann,Elizete Beatriz; Bianchi,Valmor João; Fachinello,José Carlos; Ferreira,Letícia Vanni; Oliveira,Roberto Pedroso de. This work aimed at studying the effects of different sources and cytokinin concentrations, as well as explants orientation and time of their permanence in shoots induction medium to obtain high multiplication rate. Four experiments were carried out to evaluate the sprout percentage mean, shoots number per explant, elongated shoots percentage and shoots length. The treatments with 2iP and zeatin did not promote in vitro plant multiplication. When different BAP concentrations were tested, an increase in sprout percentage was obtained using BAP concentration up to 3.66 mg L-1 (80%), and for shoots number per explant a linear behavior was observed, showing a mean of four shoots per explant with 4.0 mg L-1 BAP. For the 'Flordaguard' rootstocks, the explants... Tipo: Info:eu-repo/semantics/article Palavras-chave: Micropropagation; Cytokinin; Explant; Prunus. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100004 Crude Fermented Extract Containing Gibberellic Acid Produced by Fusarium moniliforme is an Alternative to Cost Reduction in Biofactories BABT Costa,Jefferson da Luz; Silva,André Luís Lopes da; Gollo,André Luiz; Brondani,Gilvano Ebling; Santos,Leandro Freire dos; Rodrigues,Cristine; Vandenberghe,Luciana Porto de Souza; Soccol,Carlos Ricardo. ABSTRACT Nidularium procerum and Nidularium innocentii (Bromeliaceae) were cultivated in vitro on media supplemented with different sources and levels of GA3 (gibberellic acid). These sources were the commercial powder (analytical degree) and fermented extract obtained by Fusarium moniliforme via solid state fermentation. The in vitro elongation and rooting of these plants were evaluated after 50 days of cultivation. The GA3 present in the fermented extract possess the same effect of purified GA3 (analytical degree) for the increase of the height of aerial part of shoots of N. innocentii, but not for the N. procerum being the GA3 fermented extract in a lesser degree. The GA3 fermented extract influences negatively the rooting in N. innocentii, while GA3... Tipo: Info:eu-repo/semantics/article Palavras-chave: Shelf life; Bromeliad; Gibberellin; Solid state fermentation; Micropropagation; Biofactory. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100427 Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant BABT Sharmin,Shamima Akhtar; Alam,Md. Jahangir; Sheikh,Md. Mominul Islam; Sarker,Kanak Kanti; Khalekuzzaman,Muhammad; Haque,Md. Anwarul; Alam,Mohammad Firoz; Alam,Iftekhar. In this work, plant regeneration via somatic embryogenesis was achieved from leaf and internode derived callus of Wedelia calendulacea, an endangered medicinal plant. Primary callus was induced by culturing leaf disc and internode explant on Murashige and Skoog medium supplemented with 2.0 mg L-1 of 2,4-D under light condition. Transfer of embryogenic callus on a reduced concentration of 2,4-D facilitated somatic embryo development while calluses remained unorganized at the same 2,4-D level. A histological analysis confirmed somatic embryo by revealing the presence of a closed vascular system in the developing embryos and lack of a vascularconnection with surrounding callus tissues. Somatic embryos germinated into plantlets upon transfer on MS medium... Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro; Medicinal plant; Micropropagation; Somatic embryo; Threatened; Wedelia calendulacea. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300013 In vitro Callus Induction and Plant Regeneration of Celosia argentea- An Important Medicinal Plant BABT Bakar,Dalilah Abu; Ahmed,Bakrudeen Ali; Taha,Rosna Mat. Celosia argentea (Var.) cristata (Amaranthaceae) is a widely cultivated ornamental plant, which has antibacterial, astringent, haemostatic, hypertensive, ophthalmic, and parasitic significance. This study describes a protocol for in vitro callus induction and plant regeneration from leaf and stem explants of C. argentea using Murashige and Skoog (MS) medium. Callus culture was initiated and established from seedling, leaf, and stem explants. Explants were cultured on MS medium supplemented with auxin alone (0.5 mg/L Naphthaleneacetic acid (NAA), 2, 4-Dicholorophenoxyacetic acid (2, 4-D). Green and red compact callus (98%) were induced using MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L Benzyladenine (BA). Two different concentrations (1.0 mg/L BA +... Tipo: Info:eu-repo/semantics/article Palavras-chave: Celosia argentea; Amaranthaceae; In vitro; Micropropagation; Callus; Plant regeneration. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600860 Micropropagation of an elite F1 watermelon (Citrullus lanatus) hybrid from the shoot tip of field grown plants BABT Khalekuzzaman,Mohammad; Khatun,Marufa; Rashid,Mohammad Harunur; Sheikh,Mominul Islam; Sharmin,Shamima Akhtar; Alam,Iftekhar. The aim of this work was to develop a protocol for rapid micropropagation of an elite F1 hybrid watermelon cultivar using shoot tip of field-grown plants. Maximum frequency (73%) of shoot tip showed growth response in MS medium supplemented with 5 mg l-1 benzyl adenine (BA) and 0.1 mg l-1 indole-3 acetic acid (IAA). Upon transfer to cytokinin-enriched medium, the cultures produced multiple shoots and 2.0 mg l-1 BA was optimum in this respect. Addition of gibberellic acid (GA3) in the multiplication medium resulted in better growth of shoots. Rooting rate was 100% when shoots were obtained from second subculture were cultured in medium with 1.0 mg l-1 indole-3 butyric acid (IBA). The shoots produced more roots with increasing number of subcultures. About... Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro; Watermelon; Micropropagation; Hybrid vigor. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300002 Biotic Contamination and Possible Ways of Sterilization: A Review with Reference to Bamboo Micropropagation. BABT Ray,Syandan Sinha; Ali,Nasim. ABSTRACT Multipurpose use of bamboo in rural life makes it as poor man’s timber in Asian countries. Deforestation and industrialization leads to destruction of natural forest. To replenish, a rapid plantation of bamboo could be one of the possible solutions. Bamboo is propagated mainly by vegetative methods though it is not suitable for large scale plantation because of several limitations. Micropropagation is gaining importance for large scale propagation because of its capability in raising huge number of true to type propagules in a limited space in very short span of time. Like any other plant, the chief constraint of bamboo micropropagtion is in vitro contamination arises from several sources including explants. Most of the contaminants are reduced by... Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotic contamination; Sterilization; Bamboo; Micropropagation. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100207 Assessment of Genetic Fidelity of in vitro Raised Plants in Swertia chirayita through ISSR, RAPD analysis and Peroxidase Profiling during Organogenesis BABT Sharma,Vikas; Belwal,Nidhi; Kamal,Barkha; Dobriyal,Anoop Kumar; Jadon,Vikash Singh. ABSTRACT An efficient in vitro regeneration protocol for medicinally important herb Swertia chirayita was developed and the genetic fidelity was assessed using RAPD and ISSR markers. The best shoot regeneration was observed on MS basal supplemented with 1.0 mg/L Benzyl amino purine (BAP) in combination with Indole-3-acetic acid (IAA) (0.5 mg/L) that resulted in the increase by multiplication rate (7.65) with an average of 33.33 numbers of shoots and average shoot length of 2.70 cm. It was further enhanced by the addition of adenine sulfate (0.007%) that resulted in an average of 42 shoots per clum with 4.13 cm of average shoot length and the increase in multiplication fold to 9.75 that further resulted in the reduced use of other cytokinins and auxins. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Swertia chirayita; Micropropagation; Adenine sulfate; Ascorbate peroxidase; RAPD; ISSR. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100434 Antimicrobial action of the essential oil of Lippia gracilis Schauer BABT Albuquerque,Cynthia Cavalcanti de; Camara,Terezinha Rangel; Mariano,Rosa de Lima Ramos; Willadino,Lilia; Marcelino Júnior,Cristiano; Ulisses,Cláudia. Antimicrobial activity of the essential oil (EO) of Lippia gracilis Schauer over fungi and heliconia endophytic bacteria was evaluated. For the fungi Geotrichum candidum; Trichoderma viride; Torula herbarum; Paecillomyces sp.; Fusicoccum sp.; P. aeruginens; Curvularia lunata; Aspergillus nidulans; A. flavus; and A. niger mycelial discs were inoculated in PDA medium with oil (0; 420; 440; 460; 480 e 500 µL L-1). The inhibition percentage in 420 µL L-1 was 100% for all fungi, except for C. lunata and A. niger, which was 95.58% and 89.40% , respectively. In other experiment, a suspension of the Salmonella choleraceuis-diarizonae, Enterobacter asburiae, Bacillus thuringiensis, B. pumilus, B. cereus, Klebsiella pneumoniae, and E. hormaechei was individually... Tipo: Info:eu-repo/semantics/article Palavras-chave: Micropropagation; Contamination; Monoterpenes. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500001 Factors affecting "in vitro" plant development and root colonization of sweet potato by Glomus etunicatum Becker & Gerd BJM Bressan,Wellington. Nutrients media (Murashige & Skoog, Hoagland & Arnon and White's media) supplemented or not with sucrose and substrates (vermiculite, agar and natrosol) were tested for their effects on plant development and root colonization of micropropagated sweet potato, cv. White Star, by Glomus etunicatum Becker & Gerdemann (isolate INVAM FL S329). Addition of sucrose (3%) did not affect plant development. However, hyphal growth and root colonization were depressed. Contrasting responses to media nutrient concentration were observed for plant height, root colonization, and hyphal growth. The highest concentration of nutrients in Murashige & Skoog medium improved plant development, but this medium decreased hypha growth and inhibited... Tipo: Info:eu-repo/semantics/article Palavras-chave: Arbuscular mycorrhiza; Micropropagation; Media composition; Substrate. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100006 Multiple shoot formation in Hypericum perforatum L.and hypericin production Braz. J. Plant Physiol. Santarém,Eliane Romanato; Astarita,Leandro Vieira. Hypericum perforatum is a traditional medicinal plant with wound healing and antidepressive properties. Among the secondary compounds of interest is hypericin, a naphtodianthrone that seems to participate in the medicinal effects of this species. The aim of this work was to obtain an efficient micropropagation system of H. perforatum and to compare the hypericin content between in vitro and field-grown plants. Cultures were initiated from nodal segments of mature plants inoculated onto MS medium supplemented with 4.5 muM BA, kinetin, thidiazuron, individually or in combination with 0.05 muM NAA. Organogenic explants were observed on medium with either BA or kinetin alone or in combination of these with NAA. Subculture of organogenic explants onto the... Tipo: Info:eu-repo/semantics/other Palavras-chave: Medicinal plant; Micropropagation; Organogenesis; St. John's Wort. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000100006 Influence of Vessel Type, Physical State of Medium and Temporary Immersion on the Micropropagation of Three Rhodophiala species Chilean J. Agric. Res. Muñoz,Manuel; Seemann,Peter; Jara,Gloria; Riegel,Ricardo. Rhodophiala C. Presl (Amaryllidaceae) is a genus of attractive flowering geophytes native to South America. They have ornamental value, but most species are little known and have conservation problems. The objective of this study was to optimize a micropropagation process to support the use and preservation of three Chilean native species, R. montana (Phil.) Traub, R. splendens (Rengifo) Traub., and R. ananuca (Phil.) Traub. The research evaluated the feasibility of implementing liquid medium culture and assessed the influence of different tissue culture systems on the shoot production and biomass increment of small bulbs. Three experiments were carried out. The first one determined the influences of flask size and volume of media; the second compared... Tipo: Journal article Palavras-chave: TIS; Liquid culture; Micropropagation; Acclimatization. Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000400014 Plant tissue culture: Current status, opportunities and challenges Ciencia e Investigación Agraria García-Gonzáles,Rolando; Quiroz,Karla; Carrasco,Basilio; Caligari,Peter. In the last two decades plant biotechnology applications have been widely developed and incorporated into the agricultural systems of many countries worldwide. Tissue culture tools have been a key factor to support such outcomes. Current results have allowed plant biotechnology and its products -including transgenic plants with several traits- to be the most assimilated technology for farmers and companies, representing several benefits such as: 125 millions ha of transgenic crops in 2008, the reduction of pesticides application by up to 9% in the last ten years, transgenic plants with a better nutritional quality, mass propagation of selected and healthy plants, and the production of proteins for industrial or therapeutic use. The rapid and extensive... Tipo: Journal article Palavras-chave: In vitro; Micropropagation; Organogenesis; Plant bioreactors; Somatic embryogenesis; Transgenic plants. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000300001 Registros recuperados: 113 Primeira ... 1 2 3 4 5 6 ... Última

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