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Ascorbic acid biosynthesis: a precursor study on plants Braz. J. Plant Physiol.
Barata-Soares,Anderson D.; Gomez,Maria Luiza P. A.; Mesquita,Carlos Henrique de; Lajolo,Franco M..
Since the first isolation of ascorbic acid (AsA) in 1928, few papers have been published regarding the biosynthesis of AsA in plants, especially in fruits. It took as long as 1998, before Wheeler, Jones and Smirnoff, based on a study with Arabidopsis leaves, proposed what can be considered the main pathway of biosynthesis of AsA, in which L-galactose (L-GAL) is a key precursor. This paper reports the effectiveness of some precursors (cold or radiolabeled) in the biosynthesis of AsA in different plants: green sweet pepper, white-pulp guava, red-pulp guava, papaya and strawberry at two ripening stages (mature green and ripe for papaya and mature green and half red for strawberry) and broccoli. The 'Smirnoff-Wheeler' pathway was functioning and active in all...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ascorbate; Ascorbate biosynthesis pathway; Antioxidant; Precursor infiltration.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000300004
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Expression analysis of a set of genes related to the ripening of bananas and mangoes Braz. J. Plant Physiol.
Godoy,Adriana de; Morita,Rodrigo J.; Cordenunsi,Beatriz R.; Lajolo,Franco M.; Nascimento,João Roberto O. do.
During ripening many important physic-chemical changes contribute to fruit quality, and they are precisely determined by gene expression. Specific genes are essential to normal ripening; however, information on gene expression about the majority of tropical fruit, such as bananas and mangoes is limited. In this way, the present study was undertaken with the objective to provide preliminary access to the changes in expression of some genes potentially relevant to banana and mango ripening. To simultaneously evaluate the changes in gene expression, a small collection of genes related to ethylene biosynthesis, starch mobilization, cell wall disassembly, pigment synthesis and ascorbate metabolism was assembled in nylon membranes and probed with cDNA from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fruit ripening; Reverse northern blotting.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202009000400001
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The complexity-stability hypothesis in plant gas exchange under water deficit Braz. J. Plant Physiol.
Souza,Gustavo M.; Pincus,Steven M; Monteiro,José Alberto F..
We hypothesized that more complex, i.e. irregular, temporal dynamics and a more interconnected overall network supports greater stability to gas exchange parameters (herein, CO2 net assimilation and transpiration) in plants under water deficit. To test this hypothesis two genotypes of Phaseolus vulgaris were subjected to a period of absence of irrigation, and subsequent rewatering to achieve recovery. Gas exchanges parameters were measured each 10 s during 6 h to obtain time series to evaluate complexity by Approximate Entropy (ApEn) calculations, and network connectance in each water regime. Notably, the Jalo Precoce genotype showed significantly more stability than the Guarumbé genotype under system perturbation, coincident with greater irregularity in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Approximate Entropy; Complexity; Network connectance; Plant stress theory; Photosynthesis; Temporal dynamics; Water deficit.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000400004
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Purification and structural stability of a trypsin inhibitor from Amazon Inga cylindrica [Vell.] Mart. seeds Braz. J. Plant Physiol.
Calderon,Leonardo A; Almeida Filho,Humberto A; Teles,Rozeni C. L; Medrano,Francisco J; Bloch Jr,Carlos; Santoro,Marcelo M; Freitas,Sonia M.
Inga cylindrica Trypsin Inhibitor (ICTI) was purified as a single polypeptide chain by one step anion-exchange chromatography from a crude extract of Inga cylindrica (Vell.) Mart. seeds. ICTI is a 19.5 kDa protein presenting a remarkable inhibitory activity against bovine trypsin (EC 3.4.21.4) (Ki = 4.3 nM). Circular dichroism analysis revealed that this inhibitor is a β type protein (40.4% of β-strand; 24.6% of β-turn and 6.7% of α-helix) in accordance with properties displayed in Kunitz type inhibitors. ICTI is a thermal stable protein within a wide range of pH (1.6 to 10.0) exhibiting highest stability at pH 7.0 as indicated by Tm of 70.0 ºC and ΔG25 of 48.5 ± 0.7 kJ.mol-1. The values of ΔG25 at pH 1.6 (22.5 ± 1.2 kJ.mol-1) and pH 10.0 (31.5 ± 1.0...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inga cylindrica [Vell.] Mart; Leguminosae; Mimosoideae; Protease inhibitor; Protein stability; Trypsin inhibitor.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000200001
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Ecophysiology of coconut palm under water stress Braz. J. Plant Physiol.
Gomes,Fábio P.; Prado,Carlos H.B.A..
Coconut palm is of great social and economic importance for millions of people in the tropics and subtropics. Drought is one of the main environmental factors that limit coconut productivity. In this review, physiological and morphological data are presented in an integrated perspective to provide a holistic view of the behavior of coconut trees facing water deficit under both field and controlled conditions. Great capacity to produce homorhizic roots capable of searching continuously for water and nutrients and high protoplasmic tolerance in leaves allows the coconut tree to endure water deficit. High membrane stability, osmoprotection, osmoregulation and enhanced activity of antioxidant enzymes are characteristics often found in leaves of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cocus nucifera; Desiccation tolerance; Leaf gas exchange; Leaf water relations; Net photosynthesis; Osmotic potential; Water potential; Water-use efficiency.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000400008
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Partitioning of nitrate reductase activity in Coffea arabica L. and its relation to carbon assimilation under different irradiance regimes Braz. J. Plant Physiol.
Carelli,Maria Luiza C.; Fahl,Joel I..
The distribution of in vivo nitrate reductase (EC 1.6.6.1) activity (NRA) between leaves and roots was studied in young coffee plants (Coffea arabica L.) grown in pots with watered sand in a glasshouse and irrigated with nutrient solution. The influence of irradiance regimes on the partitioning of NRA, and its relation with CO2 assimilation, was also evaluated in plants grown under approximately 20, 50 and 100% of full sunlight. Time-course of nitrate accumulation in nitrogen-starved plants showed a similar pattern in leaves and roots after supplying 15 mmol L-1 nitrate, indicating efficient ability of the roots to export nitrate to the shoot. At the same time, NRA was rapidly induced in both tissues. In shoots, NRA partitioning was synchronized among the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffee; Growth; Leaf/root ratio; Nitrate assimilation; Photosynthesis; Shading.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000300006
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Differential water uptake kinetics in axes and cotyledons during seed germination of Vigna radiata under chilling temperature and cycloheximide treatment Braz. J. Plant Physiol.
Chakraborty,R.; Kar,R.K..
Water uptake kinetics of axes and cotyledons of Vigna radiata seeds has been studied during incubation at chilling temperature (4°C) and under cycloheximide treatment. Germination rate of scarified seeds was faster than intact seeds, which can be correlated with their comparative water uptake kinetics. Chilling temperature during incubation significantly slowed down water uptake by both intact and scarified seeds. Treatment with cycloheximide was also somewhat effective in retarding water uptake, but only in scarified seeds. Water uptake by axes isolated from intact seeds (attached or detached from the cotyledons) was inhibited completely by chilling temperature as well as by cycloheximide treatment while these treatments were ineffective in preventing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chilling treatment; Cycloheximide; Germination; Water uptake; Vigna radiata seeds.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202008000400003
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Glycinebetaine improves salt tolerance in vinal (Prosopis ruscifolia Griesbach) seedlings Braz. J. Plant Physiol.
Meloni,Diego A.; Martínez,Carlos A..
Glycinebetaine (GB) is a very important organic osmolyte that accumulates in a number of diverse groups of plants in response to environmental stress. In some plants, increased resistance to drought, salinity and low temperature has been achieved through exogenous application of GB. In this study, the effect of exogenously applied GB (8 mM) on the ability of vinal (Prosopis ruscifolia G.) plants to withstand NaCl stress was investigated. The dry biomass of vinal showed a decrease under salt stress, but in GB-treated plants exposed to the same stress, this reduction was mitigated. Sodium accumulated in the leaves of plants grown under saline conditions, but the addition of GB to salt-grown plants reduced Na+ content by 40%. Salinity significantly reduced...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipid peroxidation; Salt stress; Superoxide dismutase.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202009000300007
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Phosphorus and nitrogen interaction: loss of QC identity in response to P or N limitation is antecipated in pdr23 mutant Braz. J. Plant Physiol.
Costa,Cibele T.; Strieder,Mércio L.; Abel,Stephen; Delatorre,Carla A..
Changes in root architecture are an important adaptive strategy used by plants in response to limited nutrient availability to increase the odds of acquiring them. The quiescent center (QC) plays an important role by altering the meristem activity causing differentiation and therefore, inducing a determinate growth program. The arabidopsis mutant pdr23 presents primary short root in the presence of nitrate and is inefficient in the use of nucleic acids as a source of phosphorus. In this study the effect of the pdr23 mutation on the QC maintenance under low phosphorus (P) and/or nitrogen is evaluated. QC identity is maintained in wild-type in the absence of nitrate and/or phosphate if nucleic acids can be used as an alternative source of these nutrients,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: QC25::iudA; Nutrient deficiency; Root development; Determinate root growth program; Stress.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000300006
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Nucleotide diversity of a major carotenoid biosynthetic pathway gene in wild and cultivated Solanum (Section Lycopersicon) species Braz. J. Plant Physiol.
Araújo,Ana H.; Fonseca,Maria Esther de N.; Boiteux,Leonardo S..
A polymerase chain reaction (PCR)-based approach was employed to isolate putative alleles of the chromoplast-specific lycopene beta-cyclase (CYCB) gene from wild and cultivated tomatoes [Solanum (Section Lycopersicon)]. The objective of this work was to establish an effective PCR protocol by testing DNA samples from distinct germplasm accessions with a primer pair designed to selectively target conserved regions present in the available CYCB sequences. This PCR optimization allowed the amplification of 1219 out 1666 bp of the gene in six taxa: S. cheesmaniae, S. peruvianum, S. neorickii, S. pennellii, S. pimpinellifolium and S. lycopersicum. Sixty-three mutation sites (31 transitions, 18 transversions and 14 single base deletions/insertions) were detected...
Tipo: Info:eu-repo/semantics/other Palavras-chave: CYCB; Lycopene; Lycopene-beta-cyclase; Nucleotide diversity; Tomatoes; Molecular markers.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000300007
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Effects of salt stress on plant growth, stomatal response and solute accumulation of different maize genotypes Braz. J. Plant Physiol.
Azevedo Neto,André Dias de; Prisco,José Tarquinio; Enéas-Filho,Joaquim; Lacerda,Claudivan Feitosa de; Silva,José Vieira; Costa,Paulo Henrique Alves da; Gomes-Filho,Enéas.
Seeds from eight different maize genotypes (BR3123, BR5004, BR5011, BR5026, BR5033, CMS50, D766 and ICI8447) were sown in vermiculite, and after germination they were transplanted into nutrient solution or nutrient solution containing 100 mmol.L-1 of NaCl and placed in a greenhouse. During the experimental period plant growth (dry matter, shoot to root dry mass ratio, leaf area, relative growth rate, and net assimilation rate), leaf temperature, stomatal conductance, transpiration, predawn water potential, sodium, potassium, soluble amino acids and soluble carbohydrate contents were determined in both control and salt stressed plants of all genotypes studied. Salt stress reduced plant growth of all genotypes but the genotypes BR5033 and BR5011 were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Growth analysis; Osmoregulation; Salinity; Transpiration; Zea mays; Water relations.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000100005
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EgLFY, the Eucalyptus grandis homolog of the Arabidopsis gene LEAFY is expressed in reproductive and vegetative tissues Braz. J. Plant Physiol.
Dornelas,Marcelo Carnier; Amaral,Weber A. Neves do; Rodriguez,Adriana Pinheiro Martinelli.
The EgLFY gene cloned from Eucalyptus grandis has sequence homology to the floral meristem identity gene LEAFY (LFY) from Arabidopsis and FLORICAULA (FLO) from Antirrhinum. EgLFY is preferentially expressed in the developing eucalypt floral organs in a pattern similar to that described previously for the Arabidopsis LFY. In situ hybridization experiments have shown that EgLFY is strongly expressed in the early floral meristem and then successively in the primordia of sepals, petals, stamens and carpels. It is also expressed in the leaf primordia of adult trees. The expression of the EgLFY coding region under control of the Arabidopsis LFY promoter could complement strong lfy mutations in transgenic Arabidopsis plants. These data suggest that EgLFY plays a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eucalyptus; Flowering; LEAFY; Reproductive development.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000200006
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Synergistic response of auxin and ethylene on physiology of Jatropha curcas L. Braz. J. Plant Physiol.
Joshi,Gargi; Shukla,Arvind; Shukla,Alok.
Jatropha curcas L. is a versatile crop since all its plant material is brought to use either as energy source, industrial or medicinal purpose. Several studies are ongoing in different parts of the world to optimize, enhance and exploit the growth, fruiting cycle and different developmental stages of the plant so that the economic yield of the plants can be utilized to the fullest limits. Foliar application of plant growth regulators such as ethrel (an ethylene releasing compound), indole acetic acid (IAA) and naphthalene acetic acid (NAA) at 50, 100 and 150 ppm, was found to influence different morpho-physiological characters in Jatropha curcas L, such as plant height, collar diameter, tree spread, flower initiation, number of inflorescence per plant,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Jatropha curcas; Auxin; Ethrel; Synergism; Morpho-physiological.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000100009
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Preface: Toxic metals in plants Braz. J. Plant Physiol.
Azevedo,Ricardo A.; Lea,Peter J..
Tipo: Info:eu-repo/semantics/other
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000100001
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Nitrate uptake and metabolism by roots of soybean plants under oxygen deficiency. Braz. J. Plant Physiol.
Brandão,Andrea D.; Sodek,Ladaslav.
Nitrate is reported to improve tolerance of plants towards oxygen deficiency enabled by waterlogging of the root system, but the mechanism underlying the phenomenon remains poorly understood. We studied the metabolism of nitrate in roots exposed to hypoxia, using soybean plants growing in a hydroponic system after suspending aeration and covering the surface of the nutrient solution with mineral oil. Nitrate depletion from the medium was more intense under hypoxia than normoxia, but in the presence of chloramphenicol, consumption under hypoxia was significantly reduced. Nitrite accumulated in the medium in the state of hypoxia and this effect was partially eliminated by chloramphenicol. Nitrate consumption sensitive to chloramphenicol was attributed to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Hypoxia; Nitrate reductase; Nitrite; Waterlogging.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202009000100003
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Seasonal leaf gas exchange and water potential in a woody cerrado species community Braz. J. Plant Physiol.
Prado,Carlos Henrique Britto de Assis; Wenhui,Zhang; Cardoza Rojas,Manuel Humberto; Souza,Gustavo Maia.
Predawn leaf water potential (psipd) and morning values of leaf gas exchange, as net photosynthesis (A), stomatal conductance (gs), transpiration (E), and morning leaf water potential (psimn) were determined seasonally in 22 woody cerrado species growing under natural conditions. Despite the lower mean values of psipd in the dry season (-0.35 ± 0.23 MPa) compared to the wet season (-0.08 ± 0.03 MPa), the lowest psipd in the dry season (-0.90 ± 0.00 MPa) still showed a good nocturnal leaf water status recovery for all species studied through out the year. Mean gs values dropped 78 % in the dry season, when the vapor pressure of the air was 80% greater than in the wet season. This reduction in gs led to an average reduction of 33% in both A and E, enabling...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado; Deciduousness; Network connectance; Stomatal conductance; Photosynthesis.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000100002
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Utilization of the chlorophyll a fluorescence technique as a tool for selecting tolerant species to environments of high irradiance Braz. J. Plant Physiol.
Gonçalves,José Francisco de Carvalho; Santos Júnior,Ulysses Moreira dos.
We examine the hypothesis that the chlorophyll a fluorescence technique can be an efficient tool to support the selection of species adapted to high irradiance and therefore, suitable for use in initial rehabilitation projects of degraded areas. The experiment was conducted at the Pedro de Moura Operational Base, Amazonas, Brazil. The fluorescence transients were obtained using a portable fluorometer for leaves of Bombacopsis macrocalyx (Bm), Eugenia cumini (Ec), Iryanthera macrophyla (Im) and Senna reticulata (Sr), which were subjected to high irradiance between 12:00 am and 1:00 p.m. Using the OJIP test, the following were calculated: performance index (PI ABS), density of reaction centres per cross section (RC/CS), maximum efficiency of photosystem II...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Degraded areas; Energy flux; Performance index.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000300005
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Foliar nitrogen and changes in free amino acid composition of Solanum lycopersicum under cadmium toxicity: kinetics of 15NH4+ Braz. J. Plant Physiol.
Haouari,Chiraz Chaffei; Nasraoui,Afef Hajjaji; Carrayol,Elisa; Lelendais,Maud; Ghorbel,Mohamed Habib; Gouia,Houda.
Glutamate metabolism and amino acid translocation were investigated in the control and cadmium stressed shoots of tomato (Solanum lycopersicum - 63/5 F1), using (15N) ammonium and (15N) glutamate tracers. Regardless of organ type, [15N] ammonium assimilation occurred via glutamine synthetase (EC 6.3.1.2), in the control and stressed plants, and it did not depend on glutamate dehydrogenase (EC 1.4.1.2). The [15N] ammonium and ammonium accumulation patterns support the role of glutamate dehydrogenase in the deamination of [15N] glutamate to provide 2-oxoglutarate and [15N] ammonium. In the presence of cadmium, excess [15N] ammonium was incorporated into asparagine, which served as an additional detoxification molecule. In the presence both of methionine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ammonium; Glutamate dehydrogenase; Methionine sulfoximine; 15N-NH4+.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000100004
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Monitoring the end of the in vitro phase of Anthurium andreanum Lindl. plantlets Braz. J. Plant Physiol.
Stancato,Giulio Cesare; Tucci,Maria Luiza Sant’Anna.
Estimulation of autotrophy in in vitro plantlets could be achieved through changes in the culture medium, or by changing the traditional hermetic caps by one that could allow gas exchanges between the culture and the environment. Besides that, the use of lamps with distinct emission spectrum irradiaction has propitiated successful results. This work was carried out aiming to evaluate the either the combined or the single action of some factors that can induce autotrophy on in vitro A. andraeanum cv. Eidibel plantlets. 3 sucrose concentrations were used: 0, 15 and 60 mM and for each one, to kinds of flasks according to the cap ventilation: under (0.038 L.h-1) and without ventilation. Flasks were kept under cold light fluorescent lamps or under gro-lux...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acclimatization; Araceae; Plantlets metabolism.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000100007
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Strategies to increase zinc deficiency tolerance and homeostasis in plants Braz. J. Plant Physiol.
Henriques,Ariadne Ribeiro; Chalfun-Junior,Antonio; Aarts,Mark.
Zinc deficiency is a global problem of considerable importance for agriculture and human health. Under zinc deficiency conditions, many essential zinc-dependent physiological functions are unable to operate normally, and the cellular homeostasis is adversely affected. This paper described the potential damages that low-zinc bioavailability in soil can have for plants, humans, and animals. In addition, current knowledge on physiological and molecular aspects of zinc homeostasis in plants and strategies used to increase zinc deficiency tolerance were discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofortification; Mineral nutrition; Plant abiotic stress; Plant nutrition.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000100002
Registros recuperados: 360
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