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| Registros recuperados: 978 | |
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| Vilos,Cristian; Gutiérrez,Marlen; Escobar,Roberto A; Morales,Francisco; Denardin,Juliano C; Velasquez,Luis; Altbir,Dora. |
| Background: The progress in material science and the recent advances in biodegradable/biocompatible polymers and magnetic iron oxide nanoparticles have led to develop innovative diagnostic and therapeutic strategies for diseases based on multifunctional nanoparticles, which include contrast medium for magnetic resonance imaging, agent for hyperthermia and nanocarriers for targeted drug delivery. The aim of this work is to synthesize and characterize superparamagnetic iron oxide (magnetite), and to encapsulate them into poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanoparticles for biomedical applications. Results: The magnetite nanoparticles were confirmed by X-ray diffraction and exhibited a size of 22.3 ± 8.8 nm measured by transmission electron... |
| Tipo: Journal article |
Palavras-chave: Hyperthermia; Magnetic resonance image; Magnetite; PHBV; Polymeric nanoparticles. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500008 |
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| Mezzalama,Monica; Crouch,Jonathan H; Ortiz,Rodomiro. |
| The use of transgenic crops is steadily increasing around the world, led by soybean (based on total area) and maize (in terms of total number of countries). Transgenic maize is grown in at least 17 countries across four continents: Africa, America, Asia and Europe. The comprehensive global spread of transgenic maize has significant implications for organizations involved in germplasm conservation and genetic enhancement; particularly as some countries require a GMO-free declaration when receiving shipments of maize germplasm. This article describes the protocol used by the International Maize and Wheat Improvement Center (CIMMYT) for monitoring unintentional transgene flow in maize genebank and breeding plots. The protocol is based on polymerase chain... |
| Tipo: Journal article |
Palavras-chave: Biosafety; Genetic integrity; Germplasm enhancement; GM-maize. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200006 |
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| Gatica Arias,Andrés M; Muñoz Valverde,Jenny; Ramírez Fonseca,Pilar; Valdez Melara,Marta. |
| A method for regeneration of the commercially important common bean (Phaseolus vulgaris ) using N6-benzylaminopurine(BAP) and adenine sulphate (AS) was established. Embryogenic axes of the Costa Rican common bean cultivars Bribrí, Brunca, Guaymí, Huetar and Telire were cultured on Murashige and Skoog medium supplemented with 100 mgl-1 myo-inositol, 1 mgl-1 thiamine, 30 gl-1 sucrose, BAP (0, 5 and 10 mgl-1), AS (0, 20 and 40 mgl-1) and 8 gl-1 agar. Regardless of the concentration of BAP and AS in the induction medium, the number of shoots and leaves differed significantly among the common bean cultivars evaluated. The higher average of shoots was obtained for Brunca > Telire > Bribrí > Guaymí > Huetar. Moreover, independently of the cultivar,... |
| Tipo: Journal article |
Palavras-chave: Common bean; In vitro culture; Morphogenesis; Phaseolus vulgaris L.; Plant growth regulators. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100006 |
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| Di Lorenzo,Piera; Lancioni,Hovirag; Ceccobelli,Simone; Curcio,Ludovica; Panella,Francesco; Lasagna,Emiliano. |
| Over the last 20 years, the two uniparentally inherited marker systems, namely mitochondrial DNA and Y chromosome have been widely employed to solve questions about origin and prehistorical range expansions, demographic processes, both in humans and domestic animals. The mtDNA and the Y chromosome, with their unique patterns of inheritance, continue to be extremely important source of information. These markers played significant roles in farm animals in the evaluation of the genetic variation within- and among-breed strains and lines and have widely applied in the fields of linkage mapping, paternity tests, prediction of breeding values in genome-assisted selection, analysis of genetic diversity within breeds detection of population admixture, assessment... |
| Tipo: Journal article |
Palavras-chave: Animal breeding; Bovine domestication; Cattle genetic diversity; Genetic resources; Genome-assisted selection; Inherited marker systems. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500010 |
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| Vignani,Rita; Scali,Monica; Masi,Elisa; Cresti,Mauro. |
| Microsatellite polymorphism analysis on 25 different "Sangiovese" accessions was carried out at eight microsatellite loci (VVS2, VVS4, VVS29, VVMD3, VVMD6, VVMD7, VVMD17 and VVMD21). In order to evaluate variability within the "Sangiovese" variety and to confirm variety identification, genotype analysis, allele distribution and pedigree information were processed with a DNA-automated sequencer running AlleleLinks software. DNA typing revealed three cases of genetic dissimilarity compared to registered "Sangiovese" clones and the divergent accessions thought to be different clones of the same variety. The divergent accessions were different from "Sangiovese" at four microsatellite loci (VVS2, VVS4, VVMD7 and VVMD21). An innovative non-radioactive... |
| Tipo: Journal article |
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| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000100007 |
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| Romero-Noguera,Julio; Martín-Sánchez,Inés; López-Miras,María del Mar; Ramos-López,José Miguel; Bolívar-Galiano,Fernando. |
| Since the middle of the XIX century, when dammar became popular in Occident, this natural resin is one of the most used in art painting techniques as final protective coating (varnish) as well as a component of pictorial media. The present work is the first approach to the study of the microbiological biodeterioration of this artistic material, which can seriously affect the appearance and integrity of works of art when bad conservation conditions -especially high humidity levels- take place. 12 microorganisms, fungi and bacteria, came from collection and from oil paintings affected by biodeterioration patterns, were inoculated on test specimens prepared with varnish dammar. These were incubated and analyzed by GC-MS to determine both the microbiological... |
| Tipo: Journal article |
Palavras-chave: Bacteria; Biodeterioration; Dammar; Fungi; GC-MS. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300006 |
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| Vergara-Fernández,Alberto O; Quiroz,Erich F; Aroca,Germán E; Alarcón Pulido,Nelson A. |
| In this work the variation in the toluene elimination capacity of an airlift bioreactor as a function of the toluene inlet load, using compost as the support material for the microorganisms was studied. In order to evaluate the flexibility of the reactor under changing toluene load, the toluene biodegradation was measured for flows from 2.4 x 10-2 to 0.132 m³ h-1, and a concentration range from 1.4 to 0.8 g m-3. Results show a 100% removal efficiency (RE) for minor flows, however, for a flow increase of 450% the RE decreased 40%, reflecting the equipments weak flexibility in varying flows. Meanwhile the maximum elimination capacity obtained was 230 g m-3 h-1, for toluene loads of 550 g m-3 h-1, corresponding to a flow of 0.132 m³ h-1. It was found that a... |
| Tipo: Journal article |
Palavras-chave: Airlift bioreactors; Biofiltration; Bioscrubbers; Toluene. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400003 |
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| Cimino,Cecilia; Vairo Cavalli,Sandra; Spina,Francisco; Natalucci,Claudia; Priolo,Nora. |
| The objective of this work was the optimization of the conditions of in vitro culture for callus production of Silybum marianum (L.) Gaertn. (Asteraceae). Sections of cotyledons, previously disinfected by washing successively with ethanol 70º, NaClO (10% w/v) and Tween 20 (0.05% v/v) and rinsing with sterile distilled water, were used as explants. For its initial culture, B5 medium supplemented with BA and 2,4-D solidified with phytagel was used, and a 63% survival was achieved. To obtain callus, two solid media were assayed (S1 and S2) using B5 medium supplemented with growth regulators (BA and 2,4-D or NAA and BA, respectively). The calli were grown at 25ºC during 45 days in darkness. Growth kinetics was studied using S1 medium... |
| Tipo: Journal article |
Palavras-chave: Asteraceae; Callus; Rhizogenesis; Silybum marianum. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300012 |
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| Felmer,Ricardo; Arias,María Elena. |
| Total number of cells in cloned embryos is generally lower than that of in vivo derived embryos and in bovines cell allocation at the blastocyst stage, has been observed to be affected in a large proportion of cloned embryos. The current embryo staining procedures are toxic for mammalian cells and thus can not be used to determine the developmental potential of a stained embryo. Therefore, in the present study we sought to assess the feasibility to develop a noninvasive embryo model that would be suitable for the evaluation of cloned embryos subjected to different nuclear transfer and embryo culture procedures. For doing this, we stably transfected a bovine embryonic fibroblast cell line and generated a number of clones that constitutively expressed a red... |
| Tipo: Journal article |
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| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500002 |
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| Trujillo Toledo,Luis Enrique; Gómez Riera,Raúl; Banguela Castillo,Alexander; Soto Romero,Melvis; Arrieta Sosa,Juan Gabriel; Hernández García,Lázaro. |
| The influence of N-glycosylation on the kinetic and catalytical properties of a bacterial fructosyltransferase (LsdA) produced in Pichia pastoris was studied. The glycosylated enzyme behaved similarly to non-glycosylated LsdA when substrate specificity, fructo-oligosaccharide (FOS) production, sucrose hydrolysis or levan formation reactions were carried out under different experimental conditions. The kinetic parameters for native or yeast-expressed LsdA determined at 60ºC, condition for the highest hydrolytic activity, followed a conventional Michaelis-Menten kinetics. Synthase activity of this levansucrase increased in water-restricted environments by addition of salt or organic solvent to the reaction mixtures |
| Tipo: Journal article |
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| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000200005 |
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| Valenzuela,Sofía; Balocchi,Claudio; Rodríguez,Jaime. |
| The benefits from the development of transgenic trees are expected from the improvement of traits as growth and form, wood quality, industrial processes, disease and insect resistance, herbicide tolerance, ecological restoration, rooting ability, etc. One of the first reported field trials with genetically modified forest trees was established in Belgium in 1988 and the characteristic evaluated was herbicide tolerance in poplars. Since then, there have been more than 200 reported trials, involving at least 15 forest species. The majority of the field trials have been carried out in the USA (64%). More than 50% of the field trials are done with Populus species and the main target traits are herbicide tolerance (31%), followed by marker genes (23%) and... |
| Tipo: Journal article |
Palavras-chave: Biosafety; Forestry biotechnology; GMOs; GM trees. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300031 |
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| de la Riva,Gustavo A.. |
| Agriculture is expected to feed an increasing population, forecasted to reach 8 billion by 2020, out of whom 6.7 billion will be in developing countries where the carrying capacity of agricultural lands will soon be reached. In Latin American and the Caribbean (LAC) countries, in spite of the abundance of natural resources and continued investments in development, poverty and food insecurity affect more than 55 percent of the rural population. Fifteen years ago, plant biotechnology comprised only a few applications of tissue culture, recombinant DNA technology and monoclonal antibodies. Today, genetic transformation, and marker-aided selection and breeding are just a few of the examples of the applications in crop improvement with profound implications for... |
| Tipo: Journal article |
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| Ano: 2000 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000100001 |
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| Pownall,Ian Edward. |
| This paper uses a model developed from the international political economy (IPE) school, to address the structure of the biotechnology industry. In particular, by focusing upon four primary structural elements of the industry, knowledge, production, finance and security with key secondary considerations of biosafety, public opinion and choice of intellectual property regime, the development of biotechnology companies from developed countries fits within a triad dominated industry with specific interests towards firms from developing countries. The activities of a sample of US National Association of Securities Dealers Stock Market (NASDAQ) quoted biotechnology firms are investigated to support the model which concludes by the observation of the need to... |
| Tipo: Journal article |
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| Ano: 2000 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000200004 |
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| Guacucano,Maritza; Levican,Gloria; S. Holmes,David; Jedlicki,Eugenia. |
| During the course of experiments involving RT-PCR to investigate the co-transcription of the pet and res operons of the bacterium Acidithiobacillus ferrooxidans we observed that the mRNA of certain gene pairs can be amplified by RT-PCR in the absence of added RT-primer. We provide experimental evidence that eliminates, as explanations for the observed results, the possible global contamination of the RNA preparation by genomic DNA or by contaminating single stranded DNA or RNA fragments. We suggest that the results can be explained by self-priming of the RNA perhaps as a result of secondary structure configurations that provide a suitable 3-terminus to prime the reverse transcriptase. This type of artifact can be recognized by carrying out a control in... |
| Tipo: Journal article |
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| Ano: 2000 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000300005 |
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| Registros recuperados: 978 | |
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