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Individual growth variation and its relationship with survival in juvenile Pacific oysters, Crassostrea gigas (Thunberg) ArchiMer
Boudry, Pierre; Collet, B; Mccombie, Helen; Ernande, Bruno; Morand, B; Heurtebise, Serge; Gerard, Andre.
In order to study individual growth variability and its relationship with survival in juvenile Crassostrea gigas, parental oysters were sampled at four sites located along the French Atlantic coast and bred under controlled hatchery conditions. Four groups of larvae were obtained by crossing five males and five females from each of the four sites, and a fifth group by crossing these 20 males and 20 females together in a pool. Larvae were reared under conditions allowing the maintenance of a maximum variability of size and gave five experimental groups. Oysters were individually monitored for growth and survival from 3 to 10 months after fertilization. The individual growth performances were relatively stable over time and no noticeable compensation for...
Tipo: Text Palavras-chave: Survival; Oysters; Growth; Crassostrea gigas; Aquaculture.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-467.pdf
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Modulations of the interactions between pacific cupped oyster Crassostrea gigas and Vibrio according to bacterial virulence and to genetic and physiological status of the host : Methodology and first results ArchiMer
De Decker, Sophie; Normand, Julien; Duperthuy, Marylise; Boudry, Pierre; Saulnier, Denis.
Rearing of Crassostrea gigas is the most economically important aquaculture activity in France. Bacteria belonging to Vibrio genus constitute one of the most abundant bacterial group in marine ecosystems. Two Vibrio species, V. splendidus and V. aestuarianus, were associated with many cases of mortality events in reared C. gigas spat and juvenile oysters, commonly during summer. This summer mortality syndrome has been extensively documented as the result of complex interactions between pathogens, host and environmental conditions. This work aims to study the Vibrio-host interactions and their modulations according to the virulence of pathogens and the genetic and/or physiological parameters of the host. These factors will be characterized by in vitro and...
Tipo: Text Palavras-chave: Genetic; Pathogens; Interaction; Vibrio; Crassostrea gigas; Oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3430.pdf
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Bioactive extracellular compounds produced by the dinoflagellate Alexandrium minutum are highly detrimental for oysters ArchiMer
Castrec, Justine; Soudant, P.; Payton, L.; Tran, D.; Miner, Philippe; Lambert, C.; Le Goic, N.; Huvet, Arnaud; Quillien, Virgile; Boullot, F.; Amzil, Zouher; Hegaret, Helene; Fabioux, C..
Blooms of the dinoflagellate Alexandrium spp., known as producers of paralytic shellfish toxins (PSTs), are regularly detected on the French coastline. PSTs accumulate into harvested shellfish species, such as the Pacific oyster Crassostrea gigas, and can cause strong disorders to consumers at high doses. The impacts of Alexandrium minutum on C. gigas have often been attributed to its production of PSTs without testing separately the effects of the bioactive extracellular compounds (BECs) with allelopathic, hemolytic, cytotoxic or ichthyotoxic properties, which can also be produced by these algae. The BECs, still uncharacterized, are excreted within the environment thereby impacting not only phytoplankton, zooplankton but also marine invertebrates and...
Tipo: Text Palavras-chave: Harmful algal bloom (HAB); Crassostrea gigas; Paralytic shellfish toxins (PST); Bioactive extracellular compounds (BEC); Histology; Behavior.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00435/54637/56042.pdf
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Développement et survie larvaire chez Crassostrea gigas ArchiMer
His, Edouard.
L'huître japonaise, Crassostrea gigas a été introduite dans les centres conchylicoles français principalement à partir de 1971, à la suite de l'épizootie qui a affecté C. angulata. Très rapidement cette nouvelle espèce a colonisé les zones estuariennes du littoral Atlantique français laissées vacantes par l'huître portugaise ; elle se reproduit de l'estuaire de la Bidassoa à celui de la Loire, plus occasionnellement jusqu'en Rade de Brest (LUCAS, communication personnelle). L'influence des principaux facteurs de milieu sur l'embryogénèse et la vie larvaire sera précisée. A l'aide d'exemples précis, celle des facteurs anthropiques sera mise en évidence ; enfin seront exposées les orientations des recherches qui sont développées à Arcachon, à la suite des...
Tipo: Text Palavras-chave: Huître creuse; Crassostrea gigas; Développement larvaire; Reproduction.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/00016/12705/9627.pdf
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Utilisation des élevages larvaires de Crassostrea gigas en écotoxicologie marine ArchiMer
His, Edouard; Robert, Rene.
The study of the anomalies occurring in the reproduction of Crassostrea gigas in the Arcachon Bay has been addressed through the development of some experimental protocols aimed at highlighting the action of a number of alteragenes on veligeres. The techniques used are described. They are based on the research of the action limits of the products tested on the breeding, formation and growth of oyster larvae. In addition, the sensitivity to micropollutants of the algae the veligere feed on is precised. Finally, larviculture and algae growth monitoring enabled the assessment of the quality of the sea-water and of the sediments in shellfish farming areas.
Tipo: Text Palavras-chave: Chaetoceros calcitrans; Isochysis galbana; Algues unicellulaires; Crassostrea gigas; Larves; Bio essais; Ecotoxicologie.
Ano: 1985 URL: http://archimer.ifremer.fr/doc/1985/acte-6184.PDF
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Cryopreservation of Crassostrea gigas vesicular cells: Viability and metabolic activity ArchiMer
Hanquet, Anne-caroline; Kellner, K; Heude, C; Naimi, Amine; Mathieu, M; Poncet, J.m..
Cryopreservation is widely used for long-term conservation of various tissues, embryos or gametes. However, few studies have described cryopreservation of invertebrate primary cell cultures and more particularly of marine invertebrate somatic cells. This technique would however be of great interest to facilitate the study of various metabolic processes which vary seasonally. The aim of the present study was to develop a protocol for cryopreservation of Crassostrea gigas vesicular cells. Different parameters were adjusted to improve recovery of cells after freezing. The most efficient cryoprotectant agent was a mix of Me2SO, glycerol, and ethylene glycol (4% each). The optimal cooling rate was -1 degrees C min(-1) down to -70 degrees C before transfer into...
Tipo: Text Palavras-chave: Glycogen metabolism; Vesicular cells; Cryopreservation; Mollusc; Oyster; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1938.pdf
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Mise en évidence d'une infection rickettsienne chez les huîtres ArchiMer
Comps, Michel; Bonami, Jean-robert; Vago, Constantin.
Un microorganisme procaryote a été observé dans le cytoplasme des cellules de diverticules digestifs de l'huître Crassostrea gigas . L'étude des caractéristiques du germe permet de considérer celui-ci comme appartenant aux Rickettsiales sans cycle de type chlamydien.
Tipo: Text Palavras-chave: Huître creuse; Crassostrea gigas; Pathologie; Rickettsie; Histologie.
Ano: 1977 URL: http://archimer.ifremer.fr/doc/00041/15241/12559.pdf
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Hemocyte characteristics in families of oysters, Crassostrea gigas, selected for differential survival during summer and reared in three sites ArchiMer
Lambert, Christophe; Soudant, Phillippe; Degremont, Lionel; Delaporte, Maryse; Moal, Jeanne; Boudry, Pierre; Jean, Frédéric; Huvet, Arnaud; Samain, Jean-francois.
High variability among individuals is often encountered when hemocyte characteristics are measured in bivalves. Such variability is suspected to result partly from genetic factors. In this study, hemocyte characteristics of six families of Crassostrea gigas were compared by flow cytometry at one sampling date in October 2001. These families were obtained from a nested, half-sibling cross design, and reared from July to October 2001 at three sites distributed along the French Atlantic coast from north to south: Baie des Veys (Normandy), Riviere d'Auray (Brittany) and Ronce (Marennes-oleron Basin, Poitou Charentes). Among the 15 measured hemocyte characteristics, production of reactive oxygen species (ROS) of untreated hemocytes (maintained in filtered...
Tipo: Text Palavras-chave: Genetic; Flow cytometry; Reactive oxygen species ROS; Adhesion; Hemocyte; Crassostrea gigas; Summer mortality.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3054.pdf
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Modelling growth and reproduction of the Pacific oyster Crassostrea gigas: Advances in the oyster-DEB model through application to a coastal pond ArchiMer
Bourles, Yves; Alunno-bruscia, Marianne; Pouvreau, Stephane; Tollu, Guillaume; Leguay, Didier; Arnaud, Christophe; Goulletquer, Philippe; Kooijman, S.
A bio-energetic model, based on the DEB theory exists for the Pacific oyster Crassostrea gigas. Pouvreau et al. [Pouvreau, S., Bourles, Y., Lefebvre, S., Gangnery, A., Alunno-Bruscia, M., 2006. Application of a dynamic energy budget model to the Pacific oyster, C. gigas, reared under various environmental conditions. J. Sea Res. 56, 156–167.] successfully applied this model to oysters reared in three environments with no tide and low turbidity, using chlorophyll a concentration as food quantifier. However, the robustness of the oyster-DEB model needs to be validated in varying environments where different food quantifiers reflect the food available for oysters, as is the case in estuaries and most coastal ecosystems. We therefore tested the oyster-DEB...
Tipo: Text Palavras-chave: Coastal Environment; Temperature effect; Food Quantifiers; Crassostrea gigas; Bivalves; Modelling; DEB theory.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6436.pdf
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Approche de l'activité de prédation de l'huître creuse Crassostrea gigas par les bigorneaux perceurs Ocenebra erinacea et Ocenebra inornata. Régulation des populations de perceurs Ocenebra erinacea et Ocenebra inornata : survie vis-à-vis des températures négatives et action du brûlage sur des pontes naturelles ArchiMer
Robert, Stephane; Grangere, Karine; Amiot, Jean-baptiste; Le Moine, Olivier; Soletchnik, Patrick; Guilpain, Patrice; Seugnet, Jean-luc; Geairon, Philippe; Goulletquer, Philippe; Razet, Daniel; Faury, Nicole; Garcia-meunier, Pascale; Martel, C..
Parmi les problèmes qui handicapent la production du bassin, la surexploitation du milieu trophique est souvent mise en référence (Bacher 1989, Héral, 1991) de la même façon des mortalités importantes sont régulièrement enregistrées principalement en période estivale Lodato M.I., 1997, Goulettquer, 1998, Soletchnick, 1998, 2000. Depuis la fin des années 1990 la prédation par bigorneaux perceurs est un problème important régulièrement signalé par les professionnels, au point que la section régionale a organisé un ramassage collectif des perceurs en avril 2001 . Ce problème n' est pas nouveau, dans les années 30, la prédation par Ocenebra erinacea était enregistrée pour ses ravages sur les bancs d'élevage de La Rochelle, Bourgneuf ou Arcachon (Robert S., et...
Tipo: Text Palavras-chave: Bigorneaux perceurs; Ocenebra erinacea; Ocenebra inornata; Huître creuse; Crassostrea gigas; Prédation.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00077/18791/16367.pdf
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L'ostréiculture en baie de Bourgneuf. Relation entre la croissance des huîtres Crassostrea gigas et le milieu naturel : synthèse de 1986 a 1995 ArchiMer
Barille-boyer, Anne-laure; Haure, Joel; Baud, Jean-pierre.
The oyster growth has been studied for a 10 year period, from 1986 to 1995, in four locations of the bay of Bourgneuf (French Atlantic Coast). The relations between oysters growth and the main molluscs stocks (cultivated oysters and wild mussels) or environmental factors (hydrobiology, currents, climatology) are analysed to understand the functioning of this shellfish ecosystem. As the water renewal time is about two months, primary production is essentially autochtonal. Phytoplankton development is controlled by the nutrients brought by the river Loire. An important part of the microalgac is composed by the microphytobenthos resuspended by west wind. High seston concentrations, measured on the North of the bay (150 mg/1 as an average), have a negative...
Tipo: Text Palavras-chave: Ecosystème conchylicole; Modèle global de croissance; Baie de Bourgneuf; Crassostrea gigas; Ecosystem; Shellfish culture; Global growth model; Bay of Bourgneuf; Crassostrea gigas.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/rapport-1633.pdf
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Sublethal and histopathological effects of trace levels of tributyltin fluoride on adult oysters Crassostrea gigas ArchiMer
Chagot, Dominique; Alzieu, Claude; Sanjuan, Jane; Grizel, Henri.
Shell malformations and histological effects of environmental concentrations of TBTF (2, 13.1 and 64.8 ng/!) were assessed on adult oysters Crassostrea gigas during a I-month experiment, incIuding both an exposure and a depuration phase. The results showed that the digestive gland is the primary target organ. Recoverable modifications were observed at the lowest concentration. Limited necrosis was observed at the highest concentration and a longer exposure could have led to extensive and irreversible tissue lesions. Shell malformations (chambering) were observed during the depuration phase. Finally the authors suggest that safe TBT levels in mariculture waters should be lower than 2 ng/l.
Tipo: Text Palavras-chave: Histopathological effects; Crassostrea gigas; Tributyltin; Effets histopathologiques; Crassostrea gigas; Tributylétain.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/publication-2754.pdf
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Estimation du stock d'huîtres creuses Crassostrea gigas, en élevage en Baie de Cancale, en octobre 2002 ArchiMer
Mazurie, Joseph; Bouget, Jean-francois.
in order to estimate the carrying capacity of Mont Saint-Michel Bay, the stock (or biomass) of cupped oysters, Crassostrea gigas, cultivated intertidally, in Cancale Bay, has been estimated in october 2002. Aerial photographs, at 2 scales (1/10 000 and 1/2000), provided by the mollusc farmers union, have been used to estimate the cumulative length of surelevaged trestles (« tables ») and the occupation rate by oyster bags. A field survey, between the 7th and 9th of october 2002, has been conducted by Ifremer with the help of « Lycée Maritime de Saint-Malo », on 40 sampled stations : 3 subsample bags per station were examined (counts and weights of different age-class oysters). 309 ha out of 333 leased ha were considered exploited (after elimination of...
Tipo: Text Palavras-chave: Baie du Mont Saint Michel; Cancale; Crassostrea gigas; Cupped oysters; Production; Biomass; Baie du Mont Saint Michel; Cancale; Crassostrea gigas; Huîtres creuses; Production; Biomasse; Stock.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/rapport-5296.pdf
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Epizootic diseases of oysters associated with viral infections ArchiMer
Comps, Michel.
Virus infections have been associated with major diseases of oysters of the genus Crassostrea. These infections include gill necrosis virus and hemocytic infection virus diseases of the Portuguese oyster C. angulata and more recently the oyster velar virus disease affecting larval, hatchery-reared Pacific oysters C. gigas. This report presents histo- and cytopathological characteristics of these infections in which large icosahedral cytoplasmic deoxyriboviruses, similar to the iridoviridae, are implicated, The gill necrosis virus causes, principally in the Portuguese oyster, an evolutive ulceration of the gills, including cellular hypertrophy and severe inflammation. Mortalities have been observed in the most serious cases. Hemocytic infection virus has...
Tipo: Text Palavras-chave: Crassostrea gigas; Crassostrea angulata; Oysters; Viral diseases.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/1988/publication-5912.PDF
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Etude comparée de la croissance de Crassostrea gigas Thunberg dans trois rivières du Morbihan ArchiMer
Auger, Christian; Grizel, Henri; Tige, Gilbert; Langlade, Aime; Rabouin, Marie-agnès.
Breeding Crassostrea gigas was conducted in three rivers of Morbihan (Brittany France). During one year, every four months, their increasement was examined. In one of these rivers, oysters showed, however their age and their origin, an abnormal increasement. Shells were very thick, with many chambers. At the opposite, in the two others rivers, oysters did not show that disordered state. Shells were sound and their linear increasement was harmonious
Tipo: Text Palavras-chave: Coquilles; Physiologie; Crassostrea gigas; Huîtres.
Ano: 1977 URL: http://archimer.ifremer.fr/doc/1977/acte-6245.PDF
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Single or dual experimental infections with Vibrio aestuarianus and OsHV-1 in diploid and triploid Crassostrea gigas at the spat, juvenile and adult stages ArchiMer
Azema, Patrick; Travers, Marie-agnes; Benabdelmouna, Abdellah; Degremont, Lionel.
French production of the Pacific cupped oyster, Crassostrea gigas, is currently threatened by two pathogens, OsHV-1 and V. aestuarianus. While oysters selected for their higher resistance to OsHV-1 are now available for the industry, the impact of V. aestuarianus on such oysters is unknown, especially for triploids. In addition, experimental infection has used the virus or the bacteria alone, but there have been no investigations of dual exposure to these pathogens. This study is the first report of single or dual exposure in spat (Spat1 and Spat2), juvenile and adult naïve oysters. For each of the two stocks evaluated, unselected oysters and oysters selected for their higher resistance to OsHV-1 infection were tested, as well as their triploid siblings of...
Tipo: Text Palavras-chave: Vibrio aestuarianus; OsHV-1; Dual exposure; Ploidy; Selection; Crassostrea gigas.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00347/45835/45918.pdf
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Overwiew on selective breeding and genetic improvement in bivalve shellfish ArchiMer
Boudry, Pierre.
Dr. Boudry focussed his talk on oysters, which are the most important bivalve species produced world wide (more than 4 million tons). One of the main characteristic of bivalve aquaculture is that most of the juveniles ("seed or "spat") are collected from natural recruitment (e.g. 100% of the European mussel production). Hatchery propagation is used in the case of introduced species (e.g. Crassostrea gigas in USA) or limited natural recruitment. The most significant genetic improvement for the production of Pacific oyster (C. gigas) to date has been obtained through the breeding of triploids, especially since the development of tetraploids. Triploidy lead to highly reduced gametogenesis and, as a result better growth and survival. Many quantitative...
Tipo: Text Palavras-chave: Crassostrea gigas; Oyster; Genetic variability; Bivalve shellfish; Selective breeding; Genetic.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3479.pdf
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Influence de la température sur la physiologie de l'huître Crassostrea ArchiMer
Le Gall, Jean-luc; Raillard, Olivier.
After four weeks' stabulation, at temperatures ranging from 30°C to 10°C for a given level of food (2.10 cells of Chaetoceros calcitrans.1(-1) ), a study of the growth and evolution of the biochemical contents of the oyster Crassostrea gigas showed a negative effect of high (30°C) and low (10°C) temperatures on growth; levels of total glucid and glycogen were inversely proportional to temperature and related to gametogenesis. At the same time measurements of the filtration rate, respiration and food consumption done at different levels of food (10(6) to 8.10(7) cells.1(-1) showed a better adaptation of the oysters to 20°C after one month.
Tipo: Text Palavras-chave: Crassostrea gigas; Filtration rate; Respiration; Food; Temperature; Crassostrea gigas; Filtration; Respiration; Nourriture; Température.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/1988/publication-2871.pdf
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Effect of orgnotin compounds (TBT) used in antifouling paints on cultured marine molluscs - a literature study ArchiMer
Heral, Maurice; Alzieu, Claude; Deslous-paoli, Jean-marc.
Organotin salts, used as antifouling substances have a deleterious effect on the environment particularly on molluscs and especially on the Japanese oyster Crassostrea gigas. A synthesis of the effects of tributyltin (TBT) on cultured molluscs reveals the following effects: 1) acute and chronic toxicity to the adults; 2) accumulation in the flesh 01 the adults; 3) perturbation 01 reproduction particularly fertility, mortality 01 offspring and decrease in larval growth rate; 4) decrease in the growth rate 01 juveniles and thickening 01 the shell; and 5) modification 01 the physiology al sublethal levels.
Tipo: Text Palavras-chave: TBT; Crassostrea gigas; Oyster; Marine molluscs; Organotin.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/publication-3016.pdf
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Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection ArchiMer
Corporeau, Charlotte; Tamayo, David; Pernet, Fabrice; Quere, Claudie; Madec, Stephanie.
Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 μVar) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters...
Tipo: Text Palavras-chave: Marine bivalves; Crassostrea gigas; Herpesvirus; Metabolism; Warburg.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00199/31035/29439.pdf
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