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			Ordenar por:  Relevância Autor Título Ano Registros recuperados: 230 Primeira ... 2 3 4 5 6 7 8 9 10 ... Última Optical devices evaluation for diagnosis of Plasmopara viticola CIGR Journal Aldo Calcante; Department of Agricultural Engineering, Università degli Studi di Milano, via Celoria 2- 20133 Milan; Aira Mena; Department of Agricultural Engineering, Università degli Studi di Milano, via Celoria 2- 20133 Milan; Fabrizio Mazzetto; Faculty of Science and Technology, Free University of Bozen, piazza Università, 5 – 39100 Bozen. Remote sensing (RS) is the most widely adopted technique for crop monitoring in precision viticulture (PV).  Recent research looks at the development of proximal sensing technologies alternative to RS.  The present work considers the possible use of proximal sensing optical devices for diagnosis in vineyard; in particular, we evaluated the GreenSeeker RT100 and the Crop Circle (two commercial optical sensors) in detecting different levels of grapevine downy mildew symptoms.  The analysis was conducted on vine leaves that had been picked from plants of cv.  Cabernet Franc infected by Plasmopara viticola.  Leaves were divided into homogeneous infection classes and then analyzed through the optical devices and a portable Vis/NIR (visible/near infrared)... Palavras-chave: Agricultural Engineering Precision viticulture; Diagnosis; NDVI; Proximal sensing; Optical devices. Ano: 2011 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/1827 Sporothrix schenckii and sporotrichosis Anais da ABC (AABC) Lopes-Bezerra,Leila M.; Schubach,Armando; Costa,Rosane O.. For a long time sporotrichosis has been regarded to have a low incidence in Brazil; however, recent studies demonstrate that not only the number of reported cases but also the incidence of more severe or atypical clinical forms of the disease are increasing. Recent data indicate that these more severe forms occur in about 10% of patients with confirmed diagnosis. The less frequent forms, mainly osteoarticular sporotrichosis, might be associated both with patient immunodepression and zoonotic transmission of the disease. The extracutaneous form and the atypical forms are a challenge to a newly developed serological test, introduced as an auxiliary tool for the diagnosis of unusual clinical forms of sporotrichosis. Tipo: Info:eu-repo/semantics/article Palavras-chave: Sporotrichosis; Diagnosis; Epidemiology; Drugs; Cell wall; Antigens. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000200009 A scientific survey on 1550 cases of oral lesions diagnosed in a Brazilian referral center Anais da ABC (AABC) VASCONCELOS,ARTUR C.; ABURAD,CARLOS; LIMA,IGOR F.P.; SANTOS,SHEILA M.M.; FREITAS FILHO,SILAS ANTONIO J. DE; FRANCO,ADEMIR; RODE,SIGMAR M.; PARANHOS,LUIZ R.. ABSTRACT The present study aimed to perform an epidemiological survey on the prevalence rate of oral lesions diagnosed in a referral center in Brazil and to establish association between the lesions and the age and gender of the affected patients. This analytical observational study analyzed 3521 medical records of anatomopathological exams of the Pathological Anatomy and Cytopathology Laboratory of the Cancer Hospital of the state of Mato Grosso (Brazil), from December 2011 to September 2015. A total of 1550 relevant medical records was detected. Patients aging between 41 and 60 consisted of 42.84% of the sample. Males were more affected than females (60.13%). Inflammatory processess consisted of the most prevalent diagnosis (43.29%), followed by squamous... Tipo: Info:eu-repo/semantics/article Palavras-chave: Diagnosis; Oral medicine; Pathology; Stomatology. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401691 Perception, knowledge and attitudes of Brazilian orthodontists on the treatment of Class II malocclusions Anais da ABC (AABC) KAIEDA,ARMANDO K.; LIMA,IGOR F.P.; SCANAVINI,MARCO ANTÔNIO; COQUEIRO,RAILDO S.; PITHON,MATHEUS M.; RODE,SIGMAR M.; PARANHOS,LUIZ RENATO. ABSTRACT The present study aimed to assess the perception and knowledge of Brazilian orthodontists on the ideal moment to treat Class II malocclusions. Questionnaires with open, semi-open and close questions were sent by e-mail to 1653 Brazilian orthodontists. These orthodontists were registered in the Brazilian Association of Orthodontics and Dentofacial Orthopedics (ABOR). One-hundred and three (9.86%) Orthodontists replied to the questionnaires. Most of them were males (60.5%) with specialization as the highest level of professional qualification (59.9%). Most of the orthodontists (51%) reported preference for treating Class II malocclusions in late mixed dentition, followed by the early mixed dentition (29%). The age range between 10 and 12 years old... Tipo: Info:eu-repo/semantics/article Palavras-chave: Orthodontic appliances; Attitudes; Diagnosis; Class II malocclusion. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602875 Subclinical Diabetes Anais da ABC (AABC) LIMA,LUÍS M.T.R.. ABSTRACT Type 2 diabetes mellitus (T2DM) is increasing in prevalence worldwide, and those non-diagnosed or misdiagnosed comprise a significant group compared to those diagnosed. Accumulated scientific evidence indicate that the current diagnostic markers (fasting glycemia, 2h glycemia after an oral glucose load and HbA1c) are indeed late diagnostic criteria when considering the incidence of diabetes-related complications and comorbidities, which are also at high risk in some groups among normoglycemic individuals. Additionally, the earlier identification of future risk of diabetes is desirable since it would allow better adherence to preventive actions such as lifestyle intervention, ultimately avoiding complications and minimizing the economic... Tipo: Info:eu-repo/semantics/article Palavras-chave: Diabetes; Diagnosis; Insulin; Subclinical diabetes; Metabolic syndrome. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200591 Modification of the Alere GIARDIA Ag TEST immunochromatography KIT methodology for its use in frozen fecal sediment of dogs and cats Anais da ABC (AABC) COSTA,VIVIANE A.N.; BRENER,BEATRIZ; FONSECA,ANA BEATRIZ M.; SUDRÉ,ADRIANA P.. ABSTRACT Giardia duodenalis is a worldwide intestinal parasite and is one of the most frequent protozoa species infecting dogs and cats. This study aimed to modify the methodology of Alere GIARDIA Ag TEST KIT for its use in frozen fecal sediments with different storage times in a freezer (-20°C), thus expanding the range of use of this methodology. One hundred fecal sediments from dogs (n=50) and cats (n=50) previously examined by optical microscopy for Giardia cysts were selected for this study. The agreement between the modified immunochromatography and microscopy results was calculated by Kappa coefficient. To evaluate the performance of the modified immunochromatography assay on samples with different storage time, the fecal sediments were divided into... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cats; Diagnosis; Dogs; Giardia duodenalis; Immunochromatography. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100479 Real-time PCR for detection of Brucella ovis and Histophilus somni in ovine urine and semen Arq. Bras. Med. Vet. Zootec. Moustacas,V.S.; Silva,T.M.A.; Costa,É.A.; Costa,L.F.; Paixão,T.A.; Santos,R.L.. RESUMO A epididimite infecciosa ovina é uma das principais enfermidades reprodutivas de carneiros. O presente estudo teve por objetivo desenvolver protocolos de PCR em tempo real para B. ovis e H. somni e avaliar sua aplicabilidade em amostras de sêmen e urina de carneiros. Delinearam-se primers e sondas espécie-específicos para cada agente. As sondas foram delineadas com o sistema TaqMan incorporando um marcador FAM para B. ovis e Cy5 para H. somni na extremidade 5' e um quencher na extremidade 3'. A PCR em tempo real para B. ovis e H. somni foi altamente sensível, uma vez que a amplificação de DNA ocorreu com até 0,2ng de DNA/reação. A especificidade dos iniciadores e sondas foi avaliada com amostras de DNA de outros agentes causadores de epididimite... Tipo: Info:eu-repo/semantics/article Palavras-chave: Infectious epididymitis; Ovine; Diagnosis; PCR. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601751 Meningoencephalitis in a horse associated with equine herpesvirus 1 Arq. Bras. Med. Vet. Zootec. Costa,E.A.; Lima,G.B.L.; Castro,R.T.; Furtini,R.; Portilho,R.V.; Resende,M.. Um garanhão da raça Brasileiro de Hipismo, de 12 anos de idade, apresentou um quadro neurológico complexo, que se assemelhava, em vários aspectos, aos sinais neurológicos causados pelo herpesvírus eqüino 1 (EHV-1), variando desde uma suave ataxia à completa paralisia dos posteriores. O animal morreu com menos de 12 horas após o início dos sinais nervosos. O diagnóstico de EHV-1 foi feito pela detecção do DNA viral no sistema nervoso central por meio da reação em cadeia da polimerase (PCR) e posterior sequenciamento. Tipo: Info:eu-repo/semantics/article Palavras-chave: Equine herpesvirus 1; Neurological signs; Clinical case; Diagnosis. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000600044 Aspectos clínicos e radiográficos de caprino com megaesôfago Arq. Bras. Med. Vet. Zootec. Silva Júnior,L.C.; Arruda,L.C.P.; Silva,D.G.B.; Soares,F.A.P.; Borba Neto,A.V.; Leite,J.E.B.; Soares,P.C.. A seven month old female goat showed neck swelling, apathy, appetite and weight loss, restlessness, increased salivation, cough, and regurgitation episodes which occurred post feeding. The animal was evaluated through both clinical and radiographic examinations. Plain radiography was performed and contrasted X-ray was done using barium sulfate. For radiological examination, the lateral projection was used for analysis. The results showed a radiopaque content in the esophageal lumen dorsally positioned to heart with density and appearance similar to the rumen. A dilation of the esophagus was reported and a diagnosis of megaesophagus was made. In conclusion, contrast esophagography must be included in the diagnosis of megaesophagus in goats. Tipo: Info:eu-repo/semantics/other Palavras-chave: Goat; Esophagus; Radiography; Diagnosis. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300033 Clinical diagnosis and alternative surgical treatment of tetralogy of Fallot in a dog. A case report Arq. Bras. Med. Vet. Zootec. Larsson,M.H.M.A; Pereira,L.; Jatene,F.B.; Freitas,R.F.; Barbusci,L.O.D.; Oliveira,S.M. de; Abduch,M.C.D.. Tetralogy of Fallot was diagnosed in a 30 month-old Cocker Spaniel female, through physical, radiographic, electrocardiographic, and echocardiographic exams. The animal was submitted to a palliative surgical correction, the Blalock-Taussig technique, and 46 months after the surgery, no signs of cardiopulmonary decompensation were observed. Tipo: Info:eu-repo/semantics/report Palavras-chave: Dog; Tetralogy of Fallot; Diagnosis; Surgical correction. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000500003 Winter dysentery in cows associated with Bovine Coronavirus (BCoV) Arq. Bras. Med. Vet. Zootec. Brandão,P.E.; Gregori,F.; Sforsin,A.J.; Villarreal,L.Y.B.; Jerez,J.A.. Descreve-se a pesquisa de BCoV e rotavírus em 13 mostras fecais de vacas de surtos de disenteria utilizando uma nested PCR dirigida ao gene RdRp e PAGE, respectivamente. Todas as amostras fecais foram positivas para BCoV e nenhuma delas apresentou-se positiva para rotavírus em PAGE. O encontro de coronavírus bovino em amostras fecais de vacas com disenteria sugere que este vírus possa ser o agente primário envolvido na etiologia dos casos aqui relatados. Tipo: Info:eu-repo/semantics/other Palavras-chave: Bovine; Winter dysentery; Coronavirus; PCR; Diagnosis. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000400038 Evaluation of western blotting for the diagnosis of enzootic bovine leukemia Arq. Bras. Med. Vet. Zootec. Gonzalez,E.T.; Oliva,G.A.; Norimine,J.; Cid de la Paz,V.; Echeverría,M.G.. A western blotting (WB) procedure has been developed for detecting antibodies to bovine leukosis virus (BLV) in cattle sera. Two hundred and thirty three serum samples from naturally infected cattle with BLV virus and serial bleedings from experimentally BLV infected cows were used. An agar gel immunodiffusion test (AGID) was used for comparing with the results obtained by WB. The AGID positive sera showed a different degree of reactivity by WB test against the two most important viral antigens (gp51 and p24), or against one of them. Other proteins (gp30, p15, p12 and p10) were not detected with any AGID positive sera, being observed occasionally three bands corresponding to the p24 protein. Using sera obtained by BLV experimental inoculation, the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine leukosis; Western blotting; Diagnosis. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400002 Reverse transcription-polymerase chain reaction assay for rabies virus detection Arq. Bras. Med. Vet. Zootec. Dantas Junior,J.V.; Kimura,L.M.S.; Ferreira,M.S.R.; Fialho,A.M.; Almeida,M.M.S.; Grégio,C.R.V.; Romijn,P.C.; Leite,J.P.G.. Este estudo teve por objetivo implantar um protocolo de amplificação genômica, precedida de transcrição reversa (RT-PCR) para o gene da nucleoproteína do vírus da raiva, para a utilização dessa metodologia em laboratórios onde são realizadas investigações para a detecção do vírus rábico. Foram utilizadas 50 amostras de tecido encefálico de animais (44 bovinos, 5 eqüinos e 1 quiróptero) oriundos do Estado do Rio de Janeiro, positivos por imunofluorescência direta e/ou prova biológica para o vírus rábico. A extração do RNA foi feita a partir da suspensão a 10% em PBS pH7,2 do tecido encefálico utilizando-se a metodologia de TRIzolTM (Life Technologies) e o protocolo de RT-PCR descrito por Heaton et al. (1997), incluindo algumas modificações. Dentre as 50... Tipo: Info:eu-repo/semantics/other Palavras-chave: Rabies virus; Reverse transcription-polymerase chain reaction; Diagnosis. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000300017 Comparison of two coprological methods for the veterinary diagnosis of fasciolosis Arq. Bras. Med. Vet. Zootec. Kleiman,F.; Pietrokovsky,S.; Gil,S.; Wisnivesky-Colli,C.. The sensitivity and utility of a standard faecal sedimentation method (FSM) and a modified stool sieving staining method (FSSM), both currently employed for the diagnosis of Fasciola hepatica infection were compared. Faecal samples were obtained from 51 bovines of an endemic area for fasciolosis in Southwestern Argentina. Each sample was placed in a recipient containing 5% formalin. Eight millilitres of the suspension, equivalent to 2g of faeces, were used for each of the two methods tested. The number of eggs found per sample was recorded. The proportion of positive samples obtained by the FSSM (27/51) was significantly higher than that by the FSM (11/51) (P<0.05). The percent of agreement between methods was 41%. Over a total of 27 positive samples... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; Fasciola hepatica; Coprological method; Diagnosis. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000200007 Accuracy of quantitative polymerase chain reaction in samples of frozen and paraffin-embedded healthy skin for the diagnosis of canine visceral leishmaniasis Arq. Bras. Med. Vet. Zootec. Campos,M.P.; Madeira,M.F.; Silva,D.A.; Solcà,M.S.; Espíndola,O.M.; Mendes Júnior,A.A.V.; Figueiredo,F.B.. ABSTRACT The purpose of the present work was to evaluate the accuracy of quantitative polymerase chain reaction (qPCR) performed on samples of fresh frozen tissue (FT) and formalin-fixed, paraffin-embedded (FFPE) healthy skin. This is a validation study conducted with samples from 46 dogs from an endemic area in Brazil. After sample collection, DNA extractions were conducted using commercial kits and qPCR was oriented to kinetoplast DNA (kDNA) targets of the Leishmania infantum species. The results obtained for the FFPE samples showed 63.6% sensitivity and 77.1% specificity, whereas those obtained for the FT samples showed 100% and 48.6%, respectively. Poor agreement was observed for the results of the qPCR technique with FT and FFPE samples. Our results... Tipo: Info:eu-repo/semantics/article Palavras-chave: Diagnosis; QPCR; Canine visceral leishmaniasis. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000601443 Bronchoalveolar lavage (BAL) in tapirs (Tapirus terrestris) with Mycobacterium tuberculosis Arq. Bras. Med. Vet. Zootec. Biava,J.S.; Gonçalves,R.C.; Javorouski,M.L.; Bonat,M.; Lacerda,O.; Murakami,P.S.; Vilani,R.G.D.C.; Biondo,A.W.. This paper describes the in vivo Bronchoalveolar lavage (BAL) technique by endoscopy in tapirs (Tapirus terrestris) with clinical signs of tuberculosis. The technique was performed in two tapirs, male and female, from Curitiba Zoo, Paraná, Brazil. A flexible endoscope and a polyethylene catheter were used after the chemical restraint of the animals. For BAL technique, 60mL of saline 0.9% were infused with a polyethylene catheter, introduced by the endoscope's working channel, and 15mL of BAL were recovered, analyzed and submitted to cytocentrifugation. Slides were stained by Papanicolaou, periodic acid-Schiff (PAS) and Ziehl-Neelsen methods contained high quantity of inflammatory cells on light microscopy (macrophages 27.5%, lymphocytes 0.5%, neutrophis... Tipo: Info:eu-repo/semantics/article Palavras-chave: Tapirs; Diagnosis; Endoscopy; Tuberculosis. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601643 Relevant peptides of Taenia crassiceps for the diagnosis of bovine cysticercosis by immunoblot Arq. Bras. Med. Vet. Zootec. Silva,L.F.; Pinto,P.S.A.; Ducas,C.T.S.; Santos,T.O.; Nieto,E.C.A.; Peixoto,R.P.M.G.. Given the limited knowledge about the diagnosis of bovine cysticercosis by immunoblot, the aim of this study was to assess the applicability of this test, identifying key peptides with diagnostic value. Immunoblot assays were performed using total larval antigen of Taenia crassiceps and 60 sera of positive bovines for cysticercosis (30 naturally and 30 experimentally infected with T. saginata eggs), 30 sera of negative bovines for cysticercosis and 30 sera of bovines with other diseases (fascioliasis, hydatidosis and tuberculosis). The peptides of greater diagnostic importance, in descending order of accuracy (%), were as follows: 6-8kDa (90.8%), 129-143kDa (74.2%), 99-105kDa (71.7%) and 14-19kDa (71.1%). Cross-reactions, due to fascioliasis and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine cysticercosis; Diagnosis; Immunoblot; Taenia crassiceps; Peptides. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300891 Confirmation and Sequence analysis of N gene of PPRV in South Xinjiang, China Arq. Bras. Med. Vet. Zootec. Liu,YongHong; Yang,Bo; Li,XianQiang; Wang,XueFeng; Zhang,LuYao; Li,Fei; Zhao,Li. ABSTRACT In China, Peste des petits ruminants (PPR) was officially first reported in 2007. From 2010 until the outbreak of 2013, PPRV infection was not reported. In November 2013, PPRV re-emerged in Xinjiang and rapidly spread to 22 P/A/M (provinces, autonomous regions and municipalities) of China. In the study, suspected PPRV-infected sheep in a breeding farm of South Xinjiang in 2014 were diagnosed and the characteristics of complete sequence of N protein gene of PPRV was analyzed. The sheep showed PPRV-infected signs, such as fever, orinasal secretions increase, dyspnea and diarrhea, with 60% of morbidity and 21.1% of fatality rate. The macroscopic lesions after autopsy and histopathological changes were observed under light microscope including... Tipo: Info:eu-repo/semantics/article Palavras-chave: PPR; Diagnosis; N gene; Sequence analysis. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501105 Evaluation of different sites for blood collection for β-hydroxybutyrate assessment in dairy cows using hand-held meter Arq. Bras. Med. Vet. Zootec. Alvarenga,E.A.; Moreira,G.H.F.A.; Facury Filho,E.J.; Leme,F.O.P.; Moreira,T.F.; Carvalho,A.U.. RESUMO Cetose subclínica é uma grande preocupação em rebanhos leiteiros, e seu diagnóstico e prevenção podem ter um grande impacto na saúde animal. Este estudo comparou quatro locais diferentes para a coleta de amostras de sangue (jugular, veias mamárias e coccígeas e ponta da cauda) para medição de β-hidroxibutirato (BHBA), usando-se um medidor portátil automático. Foram utilizadas seis vacas Holandesas, e a coleta de sangue foi feita no segundo, quinto, 10º, 15º e 21º dias pós-parto. Os resultados do medidor portátil foram semelhantes aos resultados do laboratório e apresentaram uma correlação forte de 0,83. As concentrações séricas de BHBA nas amostras de sangue coletadas na ponta da cauda, na jugular e na coccígea foram semelhantes. No entanto, o... Tipo: Info:eu-repo/semantics/other Palavras-chave: Dairy cows; Ketosis; Transition period; Diagnosis. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000401425 Altered indirect hemagglutination method for easy serotyping of Haemophilus parasuis Arq. Bras. Med. Vet. Zootec. Lorenson,M.S.; Miani,M.; Guizzo,J.A.; Barasoul,B.; Martínez-Martínez,S.; Rodríguez-Ferri,E.F.; Gutiérrez-Martín,C.B.; Kreutz,L.C.; Frandoloso,R.. ABSTRACT Glässer's disease is an emergent bacterial disease that affects swine husbandries worldwide causing important economic losses. The aetiological agent, Haemophilus parasuis, is currently divided in fifteen serovars but an increasing number of non-typeable serovars have been reported. Indirect hemagglutination (IHA) is indicated as a serotyping method for H. parasuis. In the present study, we describe an additional step that aims to work around a possible obstacle in the original protocol that may compromise the outcome of this assay. We observed that the choice of anticoagulant for blood collection influences and/or impairs spontaneous adsorption of H. parasuis antigens on sheep red blood cells (SRBCs). However, regardless of the anticoagulant... Tipo: Info:eu-repo/semantics/article Palavras-chave: Haemophilus parasuis; Diagnosis; Tannic acid; Serotyping; Indirect hemagglutination. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000100015 Registros recuperados: 230 Primeira ... 2 3 4 5 6 7 8 9 10 ... Última

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