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Registros recuperados: 214 | |
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Vieira-Pinto,M.; Oliveira,M.; Bernardo,F.; Martins,C.. |
This study reports the use of the fluorescent in situ hybridization (FISH) with Sal3 probe for Salmonella detection in swine carcasses inner surface (swab); and in the correspondent samples of ileum, ileocolic, and mandibular lymph nodes; and tonsils, after dilution (1:10) in buffered peptone water and a pre-enrichment step (37(0)C, 18h). In order to evaluate the efficiency of FISH, 235 naturally contaminated samples were simultaneously tested by the cultural method (ISO 6579) and by the Vitek Immuno Diagnostic Assay System (VIDAS®) - Salmonella (SLM) system. The cultural method identified 39 positive samples. From these, VIDAS®- SLM only detected 23. FISH identified 115 positive samples. This difference was highly significant (P<0.001). From positive... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Swine; Salmonella; FISH; ELFA. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000600006 |
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Isolan,L.W.; Perdoncini,G.; Todeschini,B.; Santos,L.R.; Guahyba,A.S.; Depner,R.; Nascimento,V.P.. |
RESUMO Avaliaram-se os dados do controle e monitoramento de Salmonella em carcaças de frangos de corte, antes e após a implantação do sistema de lavagem de carcaças. Foram amostradas 2692 carcaças antes da instalação do sistema e 1940 após a instalação, totalizando 4632 amostras em cinco abatedouros sob Inspeção Federal, no sul do Brasil. Anteriormente à instalação dos lavadores, obtiveram-se 156 resultados positivos para Salmonella spp. e, após a instalação, 83 resultados positivos, com diferença significativa (P<0,05/OR 1,4) entre os resultados gerais. Em dois dos cinco abatedouros avaliados, houve redução na positividade para Salmonella spp. nas carcaças amostradas após a instalação do lavador. Entretanto, em três estabelecimentos não houve diferença... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Contaminação cruzada; Controle; Monitoramento; Salmonella. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100252 |
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Oliveira,M.G.X.; Porretta,M.C.; Itaya,N.M.; Oliveira,M.C.V.; Reple,J.N.; Cunha,M.P.V.; Sanches,L.A.; Davies,Y.M.; Menão,M.C.; Borges,J.C.S.; Polaquini,L.E.M.; Knöbl,T.. |
RESUMO Os frutanos do tipo inulina são oligossacarídeos que favorecem a multiplicação de determinados gêneros bacterianos no intestino, promovendo um efeito prebiótico. Este trabalho avaliou o efeito da inulina extraída de raízes de yacon (Smallanthus sonchifolius) sobre a colonização intestinal de frangos de corte experimentalmente infectados por Salmonella Enteritidis. Sessenta frangos de corte com um dia de idade foram divididos em três grupos de tratamento, com duas repetições, criados até 21 dias. As aves do grupo yacon receberam 100mg de inulina/dia, via oral, por três dias consecutivos. No sétimo dia de vida, as aves tratadas e o controle positivo foram desafiados pela via oral com uma cultura de S. Enteritidis. Não foram observadas diferenças de... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Avicultura; Inulina; Prebióticos; Salmonella. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000300695 |
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Espinoza,Rodrigo A.; Silva‑Valenzuela,Cecilia A.; Amaya,Fernando A.; Urrutia,Ítalo M.; Contreras,Inés; Santiviago,Carlos A.. |
Abstract Background Salmonella pathogenicity island (SPI)-13 is conserved in many serovars of S. enterica, including S. Enteritidis, S. Typhimurium and S. Gallinarum. However, it is absent in typhoid serovars such as S. Typhi and Paratyphi A, which carry SPI-8 at the same genomic location. Because the interaction with macrophages is a critical step in Salmonella pathogenicity, in this study we investigated the role played by SPI-13 and SPI-8 in the interaction of S. Enteritidis and S. Typhi with cultured murine (RAW264.7) and human (THP-1) macrophages. Results Our results showed that SPI-13 was required for internalization of S. Enteritidis in murine but not human macrophages. On the other hand, SPI-8 was not required for the interaction of S. Typhi... |
Tipo: Journal article |
Palavras-chave: Salmonella; Enteritidis; Typhi; SPI-13; SPI-8; Macrophages; RAW264.7; THP-1. |
Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100402 |
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ALBUQUERQUE,Ricardo; ITO,Nair Massako Katayama; MIYAJI,Claudio Issamu. |
Durante um ano de colheita em uma fábrica comercial de ração, foram examinadas para a presença de Salmonella spp. 136 amostras de ingredientes de rações, 43 amostras de rações prontas e 110 amostras de suabes de pó. Apenas com o uso dos suabes é que não se conseguiu o isolamento. Entre os ingredientes examinados, encontrou-se salmonela em 19,85% das amostras, sendo que os subprodutos de origem animal foram mais fortemente contaminados, tendo propiciado 50% de percentual de isolamento; para os derivados lácteos, obtiveram-se 7,5%; e para outras matérias-primas, 12,5%. Foi obtido 4,65% de positividade para as rações examinadas. |
Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Ração; Salmonella; Matérias-primas; Contaminação. |
Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000600008 |
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Gautam,Vikas; Gupta,Naveen Kumar; Chaudhary,Uma; Arora,D. R.. |
BACKGROUND: Enteric fever continues to be a major public health problem, especially in the developing countries of the tropics. We determined the incidence of Salmonella bloodstream infections and their antimicrobial resistance patterns from May to August in the years 1997-2001 in Haryana, a large state of India. The minimum inhibitory concentration (MIC) was also determined for 60 isolates of S. typhi to various commonly used antimicrobial agents. MATERIAL AND METHODS: Blood cultures of 6,956 patients (PUO/septicemia) were processed by standard procedures and the Salmonella spp. isolates were identified with specific antisera and with standard biochemical tests. Antimicrobial susceptibilities were determined by Stokes disc diffusion method. The MIC of 60... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Multidrug resistance. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000600003 |
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Rushdy,Abeer Ahmed; Mabrouk,Mona Ibrahim; Abu-Sef,Ferialla Abdel-Hamid; Kheiralla,Zeinab Hassan; Abdel -All,Said Mohamed; Saleh,Neveen Mohamed. |
OBJECTIVES: To study the potential factors include gene mutation, efflux pump and alteration of permeability associated with quinolone-resistance of Salmonella enterica strains isolated from patients with acute gastroenteritis and to evaluate the degree of synergistic activity of efflux pump inhibitors when combined with ciprofloxacin against resistant isolates. METHODS: Antimicrobial resistance patterns of fifty-eight Salmonella isolates were tested. Five isolates were selected to study the mechanism of resistance associated with quinolone group, including mutation in topoisomerase-encoding gene, altered cell permeability, and expression of an active efflux system. In addition, the combination between antibiotics and efflux pump inhibitors to overcome the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Fluoroquinolone resistance; Efflux pump; Outer membrane protein; Chromosomal mutations. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000400008 |
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Castro,Fabíola Attié de; Santos,Vanda Roseli dos; Martins,Carlos H. Gomes; Fernandes,Sueli A.; Zaia,José Eduardo; Martinez,Roberto. |
Salmonella strains isolated from 1,138 samples representing 28,199 biological materials (stool, urine, blood and other fluids), collected between January 1985 and January 1999 at a reference University Hospital in Ribeirão Preto, São Paulo, Brazil, were studied. The most frequently detected serotypes were Salmonella enterica subspecies enterica serotype4,5,12:i:- (S. I 4,5,12:i:) (21.2%), S. agona (15.8%) and S. enteritidis (11.3%). A changing pattern of Salmonella serotypes was observed between 1985-1999. S. agona, which represented 27% of Salmonella serotypes isolated from 1985-1989, declined to 4% during the period from 1995 to 1999. S. enteritidis isolation remained below 1% until 1989; rose to 5.9% between 1990 and 1994, and increased to 32.3% between... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Salmonellosis; Serotypes; Epidemiology and gastroenteritis. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000500005 |
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Guo,Yiqun; Bai,Yu; Yang,Chunxia; Wang,Peng; Gu,Li. |
The mortality of patients with mycotic aneurysms is high, especially in East Asia, and infection by Salmonella species is the most common. Our study aimed to improve prognosis of adult mycotic aneurysms with early diagnosis and accurate treatment. Four adult patients with mycotic aneurysm caused by Salmonella were included and analyzed by single-center retrospective analysis. Cases reported in the literature during the past 10 years were also summarized. The average age of the 4 male patients was 61.25 years, while that of the 53 cases reported in the literature was 65.13 years. Hypertension, diabetes, and atherosclerosis were common complications. Most patients presented fever and experienced pain at the corresponding position of the aneurysm. Laboratory... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Mycotic aneurysms; EVAR. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900201 |
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Sá,Isabel Valéria Abalem de; Solari,Claude André. |
The presence of salmonellae in fecal samples or cloacal swabs of 97 pet reptiles (15 snakes, 24 lizards and 58 chelonians) was investigated. Thirty seven animals had national origin and 60 were imported. Salmonella spp was detected in 39.1% of the reptiles, being 62.5% in lizards, 53.3% in snakes and 25.8% in chelonians. Strains belonged to subspecies I (44.7%), II (10.5%), IIIa (5.2%), IIIb (21.0%) and IV (18.5%) of the enterica species, with predominance (55.3%) of subspecies usually found in cold-blooded animals (II to IV). In the subspecies I, the serovars Albany, Enteritidis and Typhimurium predominated. The Trachemys scripta elegans imported turtles corresponded to 93.3% (14/15) of the salmonellae-positive chelonians. The national iguanas presented a... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Pet reptiles. |
Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400007 |
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Nero,Luís Augusto; Mattos,Marcos Rodrigues de; Beloti,Vanerli; Barros,Marcia A.F.; Netto,Daisy Pontes; Pinto,José Paes A.N.; Andrade,Nélio José de; Silva,Wladimir P.; Franco,Bernadette D.G.M.. |
Fluctuations in the Brazilian milk market force small milk producers to find temporary trade alternatives, which include selling raw milk to people who prefer this type of milk rather than heat-processed milk. Considering the importance of these small milk producers to the market and the well-known health risks associated to consumption of raw milk, this study evaluated the microbiological quality and the presence of Listeria monocytogenes, Salmonella spp., chlorine, antimicrobials and insecticides (organophosphates and carbamates) in raw milk produced in 210 small and medium farms located in four important milk-producing Brazilian states (Paraná, São Paulo, Minas Gerais and Rio Grande do Sul). In 66% of the selected farms the milking was manual. In 33% of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hazards; Raw milk; Salmonella; Listeria monocytogenes; Hygiene indicators; Insecticides; Antimicrobials. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200007 |
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Braga,Catarina J.M.; Massis,Liliana M.; Alencar,Bruna C.G.; Rodrigues,Maurício M.; Sbrogio-Almeida,M.E.; Ferreira,Luís C.S.. |
Bacterial flagellins are important virulence-associated factors and strong inducers of inflammatory responses in mammalian hosts. Flagellins have also been investigated as potential vaccine adjuvants, either for induction of humoral or cellular immune responses, to different target antigens. In this study we investigated the adjuvant properties of three Salmonella enterica flagellins types (FliCd, FliCi and FljB) to an ovalbumin-derived CD8+ T cell-restricted epitope (OVA257264). Although mice immunized with the three tested flagellins elicited antigen-specific activated CD8+ T cells, only animals immunized with FliCi and FliCd flagellins admixed with ovalbumin mounted specific in vivo cytotoxic responses to peptide-pulsed target cells. The present results... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Flagellin; Cytotoxic T cells; Adjuvants; Vaccines. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100011 |
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Tondo,Eduardo Cesar; Machado,Taís Raquel Marcon; Malheiros,Patrícia da Silva; Padrão,Débora Kruger; Carvalho,Ana Lyl de; Brandelli,Adriano. |
The adhesion of Salmonella (S.) strains to stainless steel and polyethylene and their inactivation by biocides used in food industry was investigated. Coupons of stainless steel and polyethylene were immersed in bacterial suspensions of S. Enteritidis, S. Typhimurium, and S. Bredeney during 15, 30, and 60 minutes, and submitted to different concentrations of peracetic acid (PAA), sodium hypochlorite (NaOCl), and quaternary ammonium (Quat) sanitizers. Hydrophobicity of the surfaces was evaluated by contact angle measurements using the sessile drop method and bacterial adhesion was accompanied through bacterial counts and scanning electron microscopy (SEM). Results indicated that the three serovars of Salmonella presented similar adhesion to both materials... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Adhesion; Biocides; Stainless steel; Polyethylene. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400022 |
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Zancan,Fábio Tavares; Berchieri Junior,Angelo; Fernandes,Sueli Aparecida; Gama,Nilce Maria Soares Queiroz. |
The presence of Salmonella was investigated in transport boxes of newly hatched chicks in the moment they were received in the farms. 1,611 boxes, delivered to 15 farms (one broiler grand-parent farm, four broiler parent farms, two commercial broiler chick farms and seven laying hens farms) were tested. A total of 19 visits to these farms were done, and Salmonella was detected in ten of these visits. Salmonella Heidelberg was detected in boxes in five farms. S. Heidelberg and S. Mbandaka were simultaneously detected in one broiler breeder farm. Salmonella Enteritidis alone, S. Enteritidis and S. Mbandaka and S. Cerro alone were detected in three, one and one laying hens farms, respectively. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Day-old bird; Transport box. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300016 |
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Taban,Birce Mercanoglu; Aytac,Sait Aykut; Akkoc,Nefise; Akcelik,Mustafa. |
In the last decade, ready-to-eat (RTE) salad vegetables are gaining increasing importance in human diet. However, since they are consumed fresh, inadequate washing during processing can bring on some foodborne illnesses, like salmonellosis, since these food items have natural contamination from soil and water. During 2009-2010, a total of 81 samples were purchased arbitrarily from local markets in Ankara, and were examined for Salmonella contamination. Salmonella screening was performed by using anti-Salmonella magnetic beads system and polymerase chain reaction (PCR) identification of the suspected colonies. Then, the antibiotic resistance profiles of four Salmonella strains identified (strains RTE-1, RTE-2, RTE-3, and RTE-4) were also investigated, since... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Contamination; Plasmid-mediated sulfonamide resistance; Ready-to-eat salad vegetables; Food safety; Salmonella. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200007 |
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Registros recuperados: 214 | |
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