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| Registros recuperados: 158 | |
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| Gomes, Patricia Cristina; UEM; Ribeiro, Ricardo Pereira; UEM; Barrero, Nelson Lopera; UEM; Povh, Jayme Aparecido; UEM; Vargas, Lauro; UEM; Sirol, Rodolfo Nardez; Duke Energy International Geração Paranapanema. |
| Recentemente a produção aquícola brasileira tem apresentado grande progresso. Dentre as espécies nativas cultivadas no Brasil, a piapara (Leporinus elongatus) tem sido amplamente preconizada. Com objetivo de avaliar os programas de repovoamento, foram analisadas a variabilidade e a divergência genética de três estoques de piapara com a técnica de RAPD (Random Amplified Polymorphic). O primeiro estoque pertence à Estação de Aquicultura e Hidrologia da Duke Energy International (A); o segundo, à piscicultura de Rolândia (B) e o terceiro, ao Programa de Repovoamento dos Rios do Paraná (C). Os dez primers para RAPD utilizados produziram 105 fragmentos polimórficos, conferindo um polimorfismo de 98,1% para os três estoques avaliados. A porcentagem de locos... |
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Palavras-chave: 5.05.04.00-2 Reprodução Animal divergência genética; Locos polimórficos; Peixe; RAPD; Reprodutores; Variabilidade genética. genetic divergence; Polymorphic loci; Fish; RAPD; Reproducers; Genetic variability. |
| Ano: 2008 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/4710 |
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| Lupchinski Junior, Enio; UEM; Vargas, Lauro; UEM; Povh, Jayme Aparecido; UEM; Ribeiro, Ricardo Pereira; UEM; Mangolin, Claudete Aparecida; UEM; Barrero, Nelson Maurício Lopera; UEM. |
| Este trabalho teve como objetivo analisar, pela técnica RAPD, a variabilidade e a divergência genética de duas gerações da linhagem GIFT. Foram estimados parâmetros para os reprodutores (G0) e para a progênie (F1). A variabilidade genética foi determinada pela porcentagem de loci polimórficos e pelo índice de Shannon. As gerações apresentaram 69,6% de loci polimórficos (G0) e 60,0% de polimorfismo (F1). Os valores para o índice de Shannon foram de 0,367 para a geração G0 e de 0,317 para a F1. Os valores de divergência genética, calculados pelo teste de Mantel, foram de 0,213 para a G0 e 0,208 para a geração F1. Os resultados obtidos indicaram que houve perda da variabilidade genética da geração G0 para a F1. No entanto, um fato a ser destacado foi a alta... |
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Palavras-chave: 5.05.04.00-2 Reprodução Animal índice de Shannon; Gerações de cultivo; Divergência genética; Variabilidade genética. Shannon index; Cultivated generations; Genetic divergence; Genetic variability. |
| Ano: 2008 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/4708 |
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| Paiva, Suzana de; Universidade Estadual de Maringá; Zawadzki, Claudio Henrique; Universidade Estadual de Maringa; Ruvulo-Takasusuki, Maria Claudia Colla; Universidade Estadual de Maringá; Lapenta, Ana Silvia; Universidade Estadual de Maringá; Renesto, Erasmo; Universidade Estadual de Maringá. |
| Allozyme electrophoresis analysis were performed in four species of Hypostomus (Loricariidae), H. albopunctatus, H. hermanni, H. regani, e Hypostomus sp. 1/NUP 5612 from the Ivaí river, a tributary of the upper Paraná river. The study of 14 loci revealed diagnostic characters and exclusive alleles in a low frequency. The heterozygosity ranged from 0.000 in H. albopunctatus to 0.199 in H. hermanni, which was higher than the heterozygosity in other samples of Hypostomus in literature, as well as in other fish groups. Hypostomus albopunctatus and H. regani revealed higher similarity (I = 0.804), while H. hermanni and Hypostomus sp. 1/NUP 5612 showed the least genetic identity (I = 0.569). All samples were genetically distinguished, despite there were several... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2.02.00.00-5 catfish; Heterozygosity; Neotropical fishes; Genetic variability; Siluriformes Genética. |
| Ano: 2013 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/16355 |
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| Aguiar,Jonas; Schneider,Horacio; Gomes,Fátima; Carneiro,Jeferson; Santos,Simôni; Rodrigues,Luis R.; Sampaio,Iracilda. |
| The tambaqui, Colossoma macropomum, is the most popular fish species used for aquaculture in Brazil but there is no study comparing genetic variation among native and farmed populations of this species. In the present study, we analyzed DNA sequences of the mitochondrial DNA to evaluate the genetic diversity among two wild populations, a fry-producing breeding stock, and a sample of fish farm stocks, all from the region of Santarém, in the west of the Brazilian state of Pará. Similar levels of genetic diversity were found in all the samples and surprisingly the breeding stock showed expressive representation of the genetic diversity registered on wild populations. These results contrast considerably with those of the previous study of farmed stocks in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tambaqui; Mitochondrial DNA; Genetic variability; Aquaculture. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401439 |
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| CONTERATO,IONARA F.; SCHIFINO-WITTMANN,MARIA TERESA; GUERRA,DIVANILDE; BÜTTOW,MIRIAM V.; AGNOL,MIGUEL DALL’; DAHMER,NAIR. |
| ABSTRACT Trifolium polymorphum Poir. is an amphicarpic forage legume from southern Brazil, Uruguay, Argentina, Paraguay and Chile. Information on the genetic diversity of natural populations in natural grasslands in southern Brazil is limited. In order to increase the knowledge about this species, an analysis of the genetic diversity was carried out in 10 natural populations of T. polymorphum with the use of 20 microsatellite markers. The expected heterozygosity in T. polymorphum populations ranged from 0.40 to 0.43, with a mean of 0.42. A total of 193 alleles were detected with a mean of 9.3 alleles per locus and polymorphic information content (PIC) for these markers of 0.62 to 0.89 with a mean of 0.84.The grouping based on the Jaccard’s coefficient of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic variability; Microsatellites; Natural grasslands; Pampa biome. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401685 |
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| Silveira,Eni B.; Al-Janabi,Salah M.; Magalhães,Bonifácio P.; Carvalho,Luiz J.C.B.; Tigano,Myrian S.. |
| The genetic variability of 27 individuals from two distinct populations (Northeast and Central Brazil) of the grasshopper Schistocerca pallens (Thunberg) and eight isolates of its natural pathogen, the fungus Metarhizium flavoviride Gams & Rozsypal, was investigated using RAPD analysis. For the grasshoppers, ten different 10-mer oligonucleotide primers of arbitrary sequence were selected for analysis, resulting in 79 scorable binary characters. The program RAPDDIP applied to S. pallens revealed nucleotide diversity of 2.3 and 2.2% for the populations from Rio Grande do Norte (Northeast Brazil) and Federal District (Central Brazil), respectively. These values indicate the presence of high genetic variability within these populations. Conversely, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insecta; Genetic variability; Entomopathogenic fungus; Biological control; Molecular markers. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000100012 |
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| Tavares,R.A; Nunes,M.D; Almeida,D.B; Silva,J.C; Vaz,B.S; Moreira,C.G.A; Dionello,N.J.L; Piedras,S.R.N; Moreira,H.L.M. |
| Foram identificadas a divergência e a variabilidade genética, por meio do polimorfismo de seis marcadores microssatélites, de duas populações de Odontesthes bonariensis, utilizadas em manejos de cultivo e com potencial para fornecimento de reprodutores para programas de melhoramento genético. Do total de seis loci, cinco demonstraram eficiência para análise genética nas duas populações de O. bonariensis. A diferenciação genética significante nas populações analisadas pode fornecer a base para futuros programas de melhoramentos genéticos, através da combinação de material das populações divergentes para o desenvolvimento de linhagens ou execução de um programa de seleção. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fish; Genetic variability; Polymorphism. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000500034 |
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| SANTANNA,JULIANE R; MIYAMOTO,CLÁUDIA T; ROSADA,LÚCIA J; FRANCO,CLAUDINÉIA C S; KANESHIMA,EDILSON N; CASTRO-PRADO,MARIALBA A A. |
| The genetic variation among nine soybean-originating isolates of Colletotrichum truncatum from different Brazilian states was studied. Nitrate non-utilizing (nit) mutants were obtained with potassium chlorate and used to characterize vegetative compatibility reactions, heterokaryosis and RAPD profile. Based on pairings of nit mutants from the different isolates, five vegetative complementation groups (VCG) were identified, and barriers to the formation of heterokaryons were observed among isolates derived from the same geographic area. No complementation was observed among any of the nit mutants recovered from the isolate A, which was designed heterokaryon-self-incompatible. Based on RAPD analysis, a polymorphism was detected among the wild isolate C and... |
| Tipo: Journal article |
Palavras-chave: Genetic variability; Heterokaryosis; Parasexual recombination; RAPD. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100007 |
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| Morales-Contreras,Jonathan; Escalante,Patricia; Matías-Ferrer,Noemí. |
| Abstract The Great Curassow (Crax rubra) is a Neotropical bird with a wide distribution; it is classified under different threat categories and is listed as a vulnerable species by the IUCN. The Official Mexican Standard, the NOM-059-SEMARNAT-2010, indicates that the Great Curassow is a threatened species, and the subspecies Crax rubra griscomi, which is restricted to the island of Cozumel, is classified as critically endangered. Habitat loss and fragmentation, hunting, overexploitation, and illegal trade are among the main factors that have placed the bird at an endangered status. The objective of the present study was to determine the genetic structure and variation of the species within the Mexican populations of Crax rubra by using three mitochondrial... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cracids; Noninvasive collection; Genetic variability; Mitochondrial genes; Nuclear gene; Gene pool; Conservation. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032019000100209 |
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| Benin,Giovani; Carvalho,Fernando Irajá Félix de; Oliveira,Antonio Costa de; Marchioro,Volmir Sérgio; Vieira,Eduardo Alano; Bertan,Ivandro; Valério,Igor Pires; Marchese,José Abramo; Matei,Gilvani. |
| The use of different genetic dissimilarity indicators can help in both the selection of crop improvement strategies and as artificial crosses. The objectives of this work were to estimate the genetic dissimilarity among seven oat genotypes (Avena sativa L.) through molecular (dg) and morphological (dm) markers. Data were obtained from two experiments carried out in Capão do Leão County, RS, Brazil, in 2000. The estimated correlation coefficient (r = 0.33) indicated a low association between dg and dm estimates. The genotypes with similar pedigrees (UPF 16 and UPF 17), with a bootstrapping value of 82.7%, performed the most consistent group (dg) and clustered more closely in both techniques (dg and dm). However, genotypes with similar pedigree clustered in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Choice of parents; Genetic dissimilarity; Genetic variability. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052008000300002 |
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| Ono,Elisabete Yurie Sataque; Fungaro,Maria Helena Pelegrinelli; Sofia,Silvia Helena; Miguel,Tatiana de Ávila; Sugiura,Yoshitsugu; Hirooka,Elisa Yoko. |
| In this study a total of 16 Fusarium verticillioides strains isolated from corn feed samples were characterized by fumonisin (FB) production and random amplified polymorphic DNA (RAPD). All the strains produced FB1 and FB2 with levels ranging from 2.41 to 3996.36 µg/g, and from 1.18 to 1209.91 µg/g, respectively. From the 16 F. verticillioides strains, four were identified as low (3.59 to 1289.84 µg/g), eight as intermediate (>1289.84 to 3772.44 µg/g) and four strains as high (>3772.44 µg/g) fumonisin producers. From the total of 105 loci amplified, 60 (57.14%) were polymorphic. RAPD analysis showed very similar patterns among low, moderate and high fumonisin-producing strains. Although RAPD markers were capable of discriminating the different F.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fumonisin; Fusarium verticillioides; Genetic variability; RAPD. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400026 |
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| Kopp,Rogério Luiz; Thomaz-Soccol,Vanete; Klisiowicz,Débora do Rocio; Membrive,Norberto; Barata,José Maria Soares; Jurberg,José; Steindel,Mário; Kopp,Denize Cristina Trevisan; Castro,Edilene Alcântara de; Luz,Ennio. |
| Panstrongylus megistus is an important Chagas Disease vector and is said to be one of the species that might replace Triatoma infestans as the main vector of that disease in Brazil. The different degrees of P. megistus domiciliation in Brazil and its epidemiological relevance draw forth the need for the development of genetic studies that make it possible to analyze and understand the interchange of individual and gene fluxes among different populations. Thus, the present work aimed at studying the genetic variability of P. megistus in the State of Paraná - south of Brazil- and at comparing it with populations of the same species from five other states in Brazil (SP, MG, SC, RS, SE). In order to attain the proposed objective, 25 populations were studied... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Panstrongylus megistus; Isoenzymes; Genetic variability; Triatomines. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200012 |
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| Torezan,José Marcelo Domingues; Souza,Rogério Fernandes de; Ruas,Paulo Maurício; Ruas,Claudete de Fátima; Camargo,Elvis Hassmann; Vanzela,André Luís Laforga. |
| RAPD was used to access the genetic variability in Aspisdosperma polyneuron, a long-lived, late-reproducing tropical tree, and highly important for the Atlantic Forest. RAPD profiles from adults (pre-fragmentation, >300 years old) and seedlings (post-fragmentation, <<50 years old) were analyzed. Results showed a decrease of genetic polymorphism of post-fragmentation cohorts in small fragments and higher genetic diversity within population. The genetic diversity distribution suggested the establishment of fragments as protected reserves, and the transference of seedlings among fragments for conservation of A. polyneuron. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspidosperma; DNA markers; Forest fragmentation; Genetic variability; Peroba-rosa; RAPD. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200002 |
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| Povh,Jayme Aparecido; Ribeiro,Ricardo Pereira; Sirol,Rodolfo Nadez; Streit Jr.,Danilo Pedro; Moreira,Heden Luiz Marques; Siewerdt,Frank; Lopera-Barrero,Nelson Mauricio; Mangolin,Claudete Aparecida; Vargas,Lauro. |
| The objective of this study was to evaluate the genetic diversity and the parental contribution of Piaractus mesopotamicus in the production of offspring in the semi-natural system of reproduction. Twenty parental fishes (eleven males and nine females) and the total of 100 larvae were evaluated by microsatellite marker. The parents and offspring had thirty-one alleles and heterozygosity of 0.550 and 0.563, respectively. The females were fertilised by two up to six males while the males fertilised three up to five females. The contribution of the females and males to the offspring were 66.6 and 58%, respectively. Such results indicated no loss in the genetic variability in the offspring, and the parents had multiple paternity and reasonable contribution to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic variability; Stock enhancement programme; Reproductive management. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200018 |
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| Registros recuperados: 158 | |
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