|
|
|
| Registros recuperados: 225 | |
|
|
| Grizel, Henri; Comps, Michel; Bonami, Jean-robert; Cousserans, F.; Duthoit, Jean-louis; Le Pennec, Marie-annick. |
| Numerous diseases have been described in Ostrea and Crassostrea oysters and especially in cultivated species of commercial interest. The lack of growth, but more often mortalities, are in most cases the most obvious symptoms of this supposed sickness. The consequences of these epizooties can be so bad that they can result in the disappearance of an entire oyster population from a determined area as for Ostrea edulis L. in 1920-1921 (Dollfus, 1922) on some coasts of Europe and for Crassostrea angulata LMK in 1970-1971 on the French Atlantic coast and especially in the Marennes-Oléron and Arcachon basins. These cases can be considered as extreme cases as most of the time, oysters are only subjected to a number of episodic affections, affecting the farms only... |
| Tipo: Text |
Palavras-chave: Glande digestive; Maladie; Pathologie; Ostrea edulis; Huître. |
| Ano: 1974 |
URL: http://archimer.ifremer.fr/doc/1974/publication-6286.PDF |
| |
|
|
| Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Renault, Tristan. |
| Bonamia ostreae is an intrahaemocytic protozoan affecting Ostrea edulis. The parasite multiplies within haemocytes without being degraded and involves changes in cellular activities. Studies aiming at better understanding host response to a pathogen at the transcriptome levels are frequently based on the use of real time PCR assays, which require some reference genes. However, very few sequence data is available for O. edulis in public databases. Subtracted cDNA libraries were constructed from the O. edulis haemocytes in order to identify genes involved in host reactions against the parasite and quantitative real time PCR assays were developed to study expression of these genes. In this context, identification of reference genes and study of their... |
| Tipo: Text |
Palavras-chave: Real time PCR; Housekeeping genes; Haemocytes; Ostrea edulis; Bonamia ostreae. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00018/12880/9829.pdf |
| |
|
| |
|
|
| Lapegue, Sylvie; Harrang, Estelle; Heurtebise, Serge; Flahauw, Emilie; Donnadieu, C.; Gayral, P.; Ballenghien, M.; Genestout, L.; Barbotte, L.; Mahla, R.; Haffray, P.; Klopp, C.. |
| Use of SNPs has been favored due to their abundance in plant and animal genomes, accompanied by the falling cost and rising throughput capacity for detection and genotyping. Here, we present in vitro (obtained from targeted sequencing) and in silico discovery of SNPs, and the design of medium-throughput genotyping arrays for two oyster species, the Pacific oyster, Crassostrea gigas, and European flat oyster, Ostrea edulis. Two sets of 384 SNP markers were designed for two Illumina GoldenGate arrays and genotyped on more than 1000 samples for each species. In each case, oyster samples were obtained from wild and selected populations and from three-generation families segregating for traits of interest in aquaculture. The rate of successfully genotyped... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; GoldenGate technology; Ostrea edulis; Oysters; SNP genotyping. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00172/28369/26677.pdf |
| |
|
|
| Grizel, Henri; Tige, Gilbert. |
| The first microscope observations of the digestive gland of Ostrea edulis were conducted by Comps (1970) and Herbach (1971), but the first effects had already been observed in the autumn of 1967. During summer 1968, an abnormal mortality occurred in the upstream part of the Aber Wrach, then spread downstream and into the Aber Benoît. Similar mortalities were also observed in the area of La Tremblade and in the Arcachon Bay. Since then, a permanent monitoring is conducted in the different Breton oyster culture centres and the Arcachon area. |
| Tipo: Text |
Palavras-chave: Glande digestive; Maladie; Ostrea edulis; Huître; Pathologie. |
| Ano: 1973 |
URL: http://archimer.ifremer.fr/doc/1973/acte-5929.PDF |
| |
|
| |
|
|
| Engelsma, M; Roozenburg, I; Joly, Jean-pierre. |
| In summer 2006 an extensive mortality of Pacific oysters Crassostrea gigas occurred in Lake Grevelingen, the Netherlands. A sample of Pacific oysters was investigated for the presence of shellfish pathogens as potential causes of the mortality. Yellow-green lesions were observed in several oysters upon clinical inspection. Histopathology showed that 6 out of 36 oysters had a suspected bacterial infection, including 4 Nocardia-like infections. Two bacterial species, Vibrio aestuarianus and Nocardia crassostreae, were isolated from haemolymph samples and identified using PCR and sequencing of the 16S rRNA gene. This is the first isolation of N. crassostreae from shellfish in European waters. The near full-length 16S rRNA sequence of this Dutch Nocardia sp.... |
| Tipo: Text |
Palavras-chave: Summer mortalities; Ostrea edulis; Crassostrea gigas; Vibrio aestuarianus; Nocardia crassostreae. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4568.pdf |
| |
|
|
| Lallias, Delphine; Gomez-raya, L.; Haley, C. S.; Arzul, Isabelle; Heurtebise, Serge; Beaumont, A. R.; Boudry, Pierre; Lapegue, Sylvie. |
| We have identified quantitative trait loci (QTL) in the flat oyster (Ostrea edulis) for resistance to Bonamia ostreae, a parasite responsible for the dramatic reduction in the aquaculture of this species. An F-2 family from a cross between a wild oyster and an individual from a family selected for resistance to bonamiosis was cultured with wild oysters injected with the parasite, leading to 20% cumulative mortality. Selective genotyping of 92 out of a total of 550 F-2 progeny (i.e., 46 heavily infected oysters that died and 46 parasite-free oysters that survived) was performed using 20 microsatellites and 34 amplification fragment length polymorphism primer pairs. Both a two-stage testing strategy and QTL interval mapping methods were used. The two-stage... |
| Tipo: Text |
Palavras-chave: Statistical power; Survival analysis; QTL mapping; Disease resistance; Ostrea edulis. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6682.pdf |
| |
|
|
| Mourton, Chantal. |
| Oyster culture is, at a world scale, hit by variious epizooties, which are maintly caused by protozoans of the Phylum Ascetospora. Among them, Bonamia ostreae consitutes an appreciated model in order to study, at the cell scale, host-parasite relationships, as well as the immune role of hemocytes. Indeed, this parasite, which reproduces in hemocytes of Ostrea edulis, but cannot develop in Crassostea gigas. This development of an in vitro model for hemocyte-Bonamia relationships is presented. This includes the development and implementation of various techniques : making primocultures, protocol for infection with purified parasites, histological and immunoenzymatic analyses of infection. Such model allowed to study the interaction between host and... |
| Tipo: Text |
Palavras-chave: Modèle; Histologie; Méthodologie; Primoculture; Ostrea edulis; Parasite; Hémocytes; Bonamia ostreae; Ascetospora; Protozoaire; Epizootie. |
| Ano: 1987 |
URL: http://archimer.ifremer.fr/doc/1987/rapport-3234.pdf |
| |
|
|
| Bachere, Evelyne; Grizel, Henri. |
| A new Haplosporidie has been observed in the flat oyster Ostrea edulis from the Mediterranean Sea. Different development stages have been described in light and electron microscopy. The characteristics of the spores and especially the presence of at least four sporal filaments enable us to distinguish this Haplosporidie from those already described in the Atlantic flat oysters. Different haplosporidies have been described in the oyster. Some are known as being pathogenic, Minchinia costalis (Andrews et al., 1962 and 1978; Couch 1967) and Minchinia nelsoni (Haskin et al., 1966; Farley 1968 and 1975), others, identified only recently, are considered as occasional parasites (Van Banning, 1977). During the research conducted on Bonamia ostreae (Pichot et al.,... |
| Tipo: Text |
Palavras-chave: Pathologie; Haplosporidium; Parasite; Ostrea edulis; Huître plate. |
| Ano: 1982 |
URL: http://archimer.ifremer.fr/doc/1982/publication-6576.PDF |
| |
|
| |
|
| |
|
|
| Lapegue, Sylvie; Bedier, Edouard; Goyard, Emmanuel; Degremont, Lionel; Baud, Jean-pierre; Gerard, Andre; Goulletquer, Philippe; Boudry, Pierre. |
| Two oyster species are currently present along the French coasts : the indigenous European flat oyster (Ostrea edulis), and the Pacific cupped oyster (Crassostrea gigas), that has been introduced from Japan since the beginning of the 70ies. The flat oyster successively suffered from two protozoan diseases during the 60ies and its production decreased from 20 000 tons/year by that time to 1 500 tons/year nowadays. Consequently, the oyster production is principally (99%) based upon the Pacific oyster species with approximately 150 000 tons/year among which 90% are grown from the natural spat. However, the hatchery production of this species is developing and was estimated to 400 to 800 millions spat in 2002. Moreover, strengthened relationships between... |
| Tipo: Text |
Palavras-chave: Growth; Selection; Genetic breeding; Genetic; Ostrea edulis; Crassostrea gigas; Oysters; Croissance; Sélection; Amélioration génétique; Génétique; Ostrea edulis; Crassostrea gigas; Huîtres. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2003/acte-3491.pdf |
| |
|
|
| Gervais, Ophelie; Renault, Tristan; Arzul, Isabelle. |
| Apoptosis is a fundamental feature in the development of many organisms and tissue systems. It is also a mechanism of host defense against environmental stress factors or pathogens by contributing to the elimination of infected cells. Hemocytes play a key role in defense mechanisms in invertebrates and previous studies have shown that physical or chemical stress can increase apoptosis in hemocytes in mollusks. However this phenomenon has rarely been investigated in bivalves especially in the flat oyster Ostrea edulis. The apoptotic response of hemocytes from flat oysters, O. edulis, was investigated after exposure to UV and dexamethasone, two agents known to induce apoptosis in vertebrates. Flow cytometry and microscopy were combined to demonstrate that... |
| Tipo: Text |
Palavras-chave: Apoptosis; Hemocyte(s); Oyster; Ostrea edulis; UV; Flow cytometry; Microscopy. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00270/38160/36295.pdf |
| |
|
| |
|
|
| Fernández-boo, Sergio; Gervais, Ophelie; Prado-alvarez, Maria; Chollet, Bruno; Claverol, Stéphane; Lecadet, Cyrielle; Dubreuil, Christine; Arzul, Isabelle. |
| Bonamia ostreae is an intrahemocytic parasite that has been responsible for severe mortalities in the flat oyster Ostrea edulis since the 1970́s. The Pacific oyster Crassostrea gigas is considered to be resistant to the disease and appears to have mechanisms to avoid infection. Most studies carried out on the invertebrate immune system focus on the role of hemolymph, although mucus, which covers the body surface of molluscs, could also act as a barrier against pathogens. In this study, the in vitro effect of mucus from the oyster species Ostrea edulis and C. gigas on B. ostreae was investigated using flow cytometry. Results showed an increase in esterase activities and mortality rate of parasites exposed to mucus from both oyster species. In order to... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Crassostrea gigas; Ostrea edulis; Flow cytometry; Immune response; Proteome. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00594/70602/68795.pdf |
| |
|
|
| Lallias, Delphine; Beaumont, Andy; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Lapegue, Sylvie. |
| A breeding programme has been undertaken by Ifremer to produce selected Ostrea edulis families which are resistant to Bonamia ostreae, a parasite which is the most significant constraint on aquaculture of this oyster. Using such families, we aimed to identify Quantitative Trait Loci (QTLs) of resistance/susceptibility to bonamiosis. We used an F2 family derived from a bi-parental cross between a wild oyster and an individual from one of the selected oyster families. A total of 550 F2 individuals from the family cohabited with wild oysters that were injected with the parasite. Mortality was checked daily over six months and heart smears were used on moribund or dying oysters to detect the presence and level of infection of the parasite. Ninety two F2... |
| Tipo: Text |
Palavras-chave: QTLs; Flat oyster; Ostrea edulis; Bonamia ostreae; Genetic; QTL. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-3726.pdf |
| |
|
| |
|
|
| Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Bodoy, Alain; Heurtebise, Serge; Garnier, Jacqueline; Baud, Jean-pierre; Nourry, Max; Haure, Joel; Martin, Anne-genevieve; Claude, Serge; Barret, Jean; Devauchelle, Nicole; Joly, Jean-pierre; Goulletquer, Philippe; Coatanea, Denis; Oheix, Jocelyne; Zanette, Yvan; Blachier, Philippe. |
| This fourth part of the polyploidisation program reports the experiments that were conducted in 1992 and that were devoted to : - the acquiring of a new method using an epifluroescent microscope - the development o a new technique for inducing polyploidy - the triploidy induction for the Europena clam Ruditapes decussatus - the settlement of performance controls conducted on diploid and triploid populations of Ostrea edulis - results of performance controls conducted on diploid and triploid populations of Crassostrea gigas and Ruditapes philippinarum |
| Tipo: Text |
Palavras-chave: Ruditapes decussatus; Ruditapes philippinarum; Ostrea edulis; Crassostra gigas; Cytongenetics; Polyploidy; Ruditapes decussatus; Ruditapes philippinarum; Ostrea edulis; Crassostrea gigas; Cytogénétique; Polyploïdie. |
| Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/1993/rapport-3859.PDF |
| |
|
| |
| Registros recuperados: 225 | |
|
|
|
