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| Registros recuperados: 152 | |
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| Braga,Catarina J.M.; Massis,Liliana M.; Alencar,Bruna C.G.; Rodrigues,Maurício M.; Sbrogio-Almeida,M.E.; Ferreira,Luís C.S.. |
| Bacterial flagellins are important virulence-associated factors and strong inducers of inflammatory responses in mammalian hosts. Flagellins have also been investigated as potential vaccine adjuvants, either for induction of humoral or cellular immune responses, to different target antigens. In this study we investigated the adjuvant properties of three Salmonella enterica flagellins types (FliCd, FliCi and FljB) to an ovalbumin-derived CD8+ T cell-restricted epitope (OVA257264). Although mice immunized with the three tested flagellins elicited antigen-specific activated CD8+ T cells, only animals immunized with FliCi and FliCd flagellins admixed with ovalbumin mounted specific in vivo cytotoxic responses to peptide-pulsed target cells. The present results... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Flagellin; Cytotoxic T cells; Adjuvants; Vaccines. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100011 |
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| Vieira,Regine Helena Silva dos Fernandes; Rodrigues,Dália dos Prazeres; Menezes,Everardo A.; Evangelista,Norma Suely de Santana; Reis,Eliane M.F. dos; Barreto,Leopoldo Melo; Gonçalves,Flávia Araújo. |
| The presence of faecal contamination and pathogenic microorganisms in samples of dry and wet sand collected from three major beaches in Fortaleza, Ceará State, Brazil: (Praia do Mucuripe, Praia do Futuro and Praia do Caça e Pesca), during the period of May 1999 to January 2000 was evaluated. Praia do Caça e Pesca had the highest incidence of E. coli in dry sand (56%) followed by Praia do Mucuripe (28%) and Praia do Futuro (16%). In wet sand, results were 48%, 28% and 24% for Praia do Caça e Pesca, Praia do Futuro and Praia do Mucuripe, respectively. Only two samples from Praia do Futuro, one from dry sand and another one from wet sand, were positive for Salmonella. V. parahaemolyticus was isolated from four samples from Praia do Caça e Pesca (two from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sand contamination; Yeasts; Faecal coliforms; Vibrio parahaemolyticus; Salmonella. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200001 |
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| Garrido,Antonio Marin; Burgos,Mª Jose Grande; Márquez,Mª Luisa Fernández; Aguayo,Mª Carmen López; Pulido,Rubén Pérez; Árbol,Julia Toledo del; Gálvez,Antonio; López,Rosario Lucas. |
| Salmonella serovars sampled from meat products in Southern Spain (Andalucía) during the period 2002-2007 were analyzed in this study. The serovars most frequently detected (in order) were Typhimurium, Enteritidis, Derby, Anatum and Rissen. Isolates (n = 43) were tested for sensitivity to biocides, including the quaternary ammonium compounds benzalkonium chloride (BC), cetrimide (CT) and hexadecylpyridinium chloride (HDP), and the bisphenols triclosan (TC) and hexachlorophene (CF). The minimum inhibitory concentration (MIC) for the quaternary ammonium compounds was in the range of 25 to 50 mg/L for most isolates, although a few isolates required much higher concentrations, up to 250 mg/L. Bisphenols showed higher inhibitory activity, with a MIC of 2.5 to 25... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Biocides; Serovars; Meat; Chorizo. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401177 |
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| Djordjević,Jasna; Bošković,Marija; Starčević,Marija; Ivanović,Jelena; Karabasil,Nedjeljko; Dimitrijević,Mirjana; Lazić,Ivana Branković; Baltić,Milan Ž.. |
| Abstract The effect of different modified atmosphere packaging regimes on the behavior of Salmonella spp. on minced meat was studied. Minced meat was experimentally contaminated with a Salmonella spp. cocktail (S. Enteritidis, S. Typhimurium, S. Infantis and S. Arizonae), packaged under vacuum or modified atmosphere with initial headspaces containing 20%O2/50%CO2/30%N2 and 20%O2/30%CO2/50%N2) and stored at 3 ± 1 °C for 12 days. Samples were analyzed for Salmonella spp., viable and lactic acid bacteria count every third day. Salmonella spp. counts decreased during storage in all packaging types, with reductions of about 1.5 log CFU/g. A significant difference (p < 0.01) was noted between Salmonella spp. counts in meat packaged in vacuum and modified... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Minced meat; Modified atmosphere packaging; Vacuum; Salmonella; PH. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300607 |
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| Duarte,Dalila Angélica Moliterno; Ribeiro,Aldemir Reginato; Vasconcelos,Ana Mércia Mendes; Santos,Sylnei Barros; Silva,Juliana Vital Domingos; Andrade,Patrícia Lúcia Arruda de; Falcão,Lúcia Sadae Pereira da Costa de Arruda. |
| The present study was carried out to evaluate the occurrence of Salmonellae in broiler chicken carcasses and to determine the antimicrobial resistance profile of the isolated strains. Twenty-five out of the 260 broiler chicken carcasses samples (9.6%) were positive for Salmonella. S. Enteritidis was the most frequent serovar. Nineteen Salmonella isolates were tested for antimicrobial resistance, and the results indicated that 94.7% were resistant to at least one antimicrobial agent. Resistance to streptomycin (73.7%), nitrofurantoin (52.3%), tetracycline (31.6%), and nalidixic acid (21%) were the prevalent amongst Salmonella strains tested. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Broiler chicken carcasses; Antimicrobial resistance; Salmonella. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300020 |
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| Tondo,Eduardo Cesar; Machado,Taís Raquel Marcon; Malheiros,Patrícia da Silva; Padrão,Débora Kruger; Carvalho,Ana Lyl de; Brandelli,Adriano. |
| The adhesion of Salmonella (S.) strains to stainless steel and polyethylene and their inactivation by biocides used in food industry was investigated. Coupons of stainless steel and polyethylene were immersed in bacterial suspensions of S. Enteritidis, S. Typhimurium, and S. Bredeney during 15, 30, and 60 minutes, and submitted to different concentrations of peracetic acid (PAA), sodium hypochlorite (NaOCl), and quaternary ammonium (Quat) sanitizers. Hydrophobicity of the surfaces was evaluated by contact angle measurements using the sessile drop method and bacterial adhesion was accompanied through bacterial counts and scanning electron microscopy (SEM). Results indicated that the three serovars of Salmonella presented similar adhesion to both materials... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Adhesion; Biocides; Stainless steel; Polyethylene. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400022 |
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| Schneid,Andréa dos Santos; Rodrigues,Kelly Lameiro; Chemello,Davi; Tondo,Eduardo Cesar; Ayub,Marco Antônio Zacchia; Aleixo,José Antonio Guimarães. |
| In this work, an indirect ELISA based on a monoclonal antibody (MAb) specific for an outer membrane protein of Salmonellaenterica serovar Enteritidis was used for detection of Salmonella in 154 samples of chicken meat. Its efficiency was determined through comparison with the results obtained from the conventional method. The prevalence of samples contaminated with Salmonella was 23% with the conventional culture method, and 26% with the ELISA. From thirty-five samples positive for Salmonella by the conventional method, 33 were also positive by ELISA. Seven other samples were only positive in the ELISA. Comparison of the results obtained in the two methods showed an ELISA sensitivity and specificity of 94%, and positive and negative predictive values of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Monoclonal antibody; Policlonal antibody; ELISA. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300027 |
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| Matias,Carlos Alexandre Rey; Pereira,Ingrid Annes; Reis,Eliane Moura Falavina dos; Rodrigues,Dália dos Prazeres; Siciliano,Salvatore. |
| Abstract The illegal wildlife trade may increase the risk of infectious disease transmission, and it may not only cause disease outbreaks in humans but also threaten livestock, native wild populations, and ecosystems' health. Bird species may act as carriers in the transmission of enteric pathogens. However, epidemiological studies on zoonotic bacteria in wild birds are rare in Brazil. From March 2011 to March 2012, we investigated the frequency of Enterobacteriaceae in cloacal swab samples from 109 birds of the passerine and Psittacidae families. These birds were recovered from illegal trade in Rio de Janeiro, Brazil, and sent to a rehabilitation center. Gram-negative bacteria were isolated from 86 wild birds (78.9%). A mean (±SD) of 1.68 (±1.30)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antibiotic resistance; Enterobacteriaceae; Public health; Salmonella; Wild birds. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400882 |
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| Revolledo,Liliana; Ferreira,Claudete Serrano Astolfi; Ferreira,Antonio José Piantino. |
| The efficacy of three different types of experimental competitive exclusion (CE-A, CE-B and CE-C) cultures against Salmonella Kedougou (SK) NCTC 12173, resistant to nalidixic-acid resistant (NalR), in one-day-old broiler chicks, in four treatments with three replicates in each treatment, was evaluated. The mean logarithmic counts of Salmonella program of feces were 0.4 L in the group treated with CE-A, derived from the whole cecal contents of an adult bird with a mixture of aerobic and anaerobic bacterial cultures; 1.22 in the group treated with CE-B, containing aerobic bacterial culture; 1.00 in the group treated with CE-C, containing anaerobic bacterial culture; and 6.64 in the control group. The percentage of colonized birds varied from 10% to 26.66% in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Competitive exclusion; Salmonella; Broiler chicks. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400014 |
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| Carvalho,Angela Cleusa de Fátima Banzatto de; Cortez,Ana Lígia Lordello. |
| Alimentos de origem animal representam papel fundamental na epidemiologia das salmoneloses humanas. Apesar dos avanços tecnológicos, a carne de frango ainda é passível de contaminação bacteriana, especialmente por microrganismos do gênero Salmonella, que podem encontrar-se albergados no trato intestinal ou em outra parte do corpo das aves. O presente trabalho objetivou pesquisar a ocorrência de Salmonella em carne de frango e derivados procedentes da região Nordeste do Estado de São Paulo. Foram analisadas, através do método convencional de cultivo, 45 amostras de carcaças, 60 de carne mecanicamente separada (CMS), 25 de lingüiça de frango, 20 de peito, e 15 de coxa e sobre-coxa. Salmonella spp. foi encontrada em 13,3% (6/45) das carcaças, 25% (15/60) das... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carne mecanicamente separada; Frango; Salmonella. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000600040 |
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| Borsoi,Anderlise; Moraes,Hamilton Luis de Souza; Salle,Carlos Tadeu Pippi; Nascimento,Vladimir Pinheiro do. |
| A Salmonella permanece um importante problema na avicultura e, considerando os patógenos transmitidos por alimentos, aparece como um dos agentes principais em surtos de toxinfecções alimentares. Para auxiliar na avaliação de riscos em adquirir infecção alimentar via carne de frangos que sofreram cocção inadequada, ou através de contaminação cruzada a partir desses animais, torna-se importante determinar a extensão de contaminação por patógenos em carne crua. No presente trabalho, foram analisadas 180 carcaças de frangos resfriadas, adquiridas em varejos, para pesquisa de Salmonella com determinação do número de células da bactéria. Foi utilizado o método do número mais provável (NMP) nos ágares para isolamento verde brilhante com novobiocina (BGN) e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; NMP; Carcaça de frango; Patógeno alimentar. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001100014 |
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| Loguercio,Andrea Pinto; Aleixo,José Antonio Guimarães; Vargas,Agueda Castagna de; Costa,Mateus Matiuzzi da. |
| Um teste ELISA, baseado em um anticorpo monoclonal (MAb) específico para uma proteína de membrana externa de Salmonella enterica serovar Enteritidis foi comparado com o método de cultivo tradicional na detecção de Salmonella spp. em 110 amostras de lingüiça suína frescal. A prevalência do patógeno nas amostras foi de 11,82% de acordo com o cultivo tradicional. O teste ELISA revelou sensibilidade, especificidade, valores preditivos positivos e negativos de 100%, 98%, 87% e 100%, respectivamente. Tais achados indicam que, comparado ao sistema tradicional, o teste imunológico foi bastante eficaz na determinação de Salmonella spp. em amostras de lingüiças naturalmente contaminadas. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; ELISA; Lingüiça suína. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000600022 |
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| Salvatori,Rosângela Uhrig; Bessa,Marjo Cadó; Cardoso,Marisa Ribeiro de Itapema. |
| Noventa e três amostras de embutidos coletados no Mercado Público Central de Porto Alegre foram analisadas quanto à presença de Salmonella sp. e à contagem de coliformes fecais. Todos as amostras apresentaram ausência de Salmonella sp., enquanto, em relação aos coliformes fecais, constatou-se que quatorze amostras de embutidos frescais encontravam-se acima do padrão estabelecido pela portaria n° 451, em vigor no momento da coleta. Entretanto, se fossem considerados os parâmetros estabelecidos na Resolução n° 12, em vigor atualmente, apenas cinco amostras seriam consideradas fora de padrão. A maioria das amostras com contagens de coliformes fecais acima de 5x10²/g estava armazenadas acima da temperatura adequada no momento da coleta. |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Salmonella; Embutidos; Coliformes fecais. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000400031 |
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| Registros recuperados: 152 | |
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