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	Provedor de dados Infoteca-e (1) Autor CALBO, A. G. (1) SILVA, W. L. de C e. (1) Palavra-chave Irrigas (1) Manejo de irrigação. (1) Manejo de Água. (1) Tipo do documento Livro técnico (INFOTECA-E) (1) Ano 2005 (1) País Brazil (1) Idioma Português (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 174 Primeira ... 1 2 3 4 5 6 7 8 9 ... Última Molecular characterisation of Aspergillus flavus isolates from peanut fields in India using AFLP BJM Singh,Diwakar; Radhakrishnan,T.; Kumar,Vinod; Bagwan,N.B.; Basu,M.S.; Dobaria,J.R.; Mishra,Gyan P.; Chanda,S.V.. Aflatoxin contamination of peanut, due to infection by Aspergillus flavus, is a major problem of rain-fed agriculture in India. In the present study, molecular characterisation of 187 Aspergillus flavus isolates, which were sampled from the peanut fields of Gujarat state in India, was performed using AFLP markers. On a pooled cluster analysis, the markers could successfully discriminate among the ‘A’, ‘B’ and ‘G’ group A. flavus isolates. PCoA analysis also showed equivalent results to the cluster analysis. Most of the isolates from one district could be clustered together, which indicated genetic similarity among the isolates. Further, a lot of genetic variability was observed within a district and within a group. The results of AMOVA test revealed that... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; AMOVA; ELISA; Genetic diversity; Groundnut; PCA. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300673 Characterization of Mycoplasma penetrans and Mycoplasma fermentans immunodominant proteins BJM Bruder,Juliana; Godry,Renata Cristina; Takei,Kioko; Cunha,Regina Ayr Florio da. Mycoplasmas are a heterogeneous group of the smallest organisms capable of self replication and are known to cause many detrimental diseases in both animals and humans. These wall-less prokaryotes are enveloped by a lipoprotein membrane and their small genomes are sufficient to synthesize molecules required for growth and self-replication. Among sixteen species isolated from humans, Mycoplasma pneumoniae, an agent of primary atypical pneumonia, and the urogenital tract species Mycoplasma hominis,Ureaplasma urealyticum and Ureaplasma parvum have been confirmed to be pathogenic. Mycoplasma penetrans and Mycoplasma fermentans, which are species associated with HIV, have been investigated mainly in research laboratories. In this study we have characterized... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoplasma penetrans; Mycoplasma fermentans; LAMP-proteins; ELISA; Western blot. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200007 Comparison of serological methods for the diagnostic of Caprine arthritis-encephalitis (CAE) in Rio de Janeiro, Brazil BJM Cortez-Moreira,Madelayne; Oelemann,Walter M.R.; Lilenbaum,Walter. Serum samples of 562 goats were tested for Caprine arthritis-encephalitis using agar gel immunodiffusion test (AGID) and enzyme linked immunosorbent assay (ELISA). Seventy-nine samples (14.1%) were reactive by both testes but 141 (25.1%) were reactive only by ELISA. ELISA, when compared to AGID, presented 100% sensitivity and 70.8% specificity. Tipo: Info:eu-repo/semantics/article Palavras-chave: Caprine arthritis-encephalitis; CAE; Goats; ELISA; AGID. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100010 Expression of enterovirus 71 capsid protein VP1 in Escherichia coli and its clinical application BJM Shi,Mei; Zhou,Yaping; Cao,Limin; Ding,Cuijun; Ji,Yun; Jiang,Qinbo; Liu,Xiping; Li,Xiang; Hou,Xueling; Peng,Hongjun; Shi,Weifeng. The VPl gene of enterovirus 71 (EV71) was synthesized, construct a recombinant plasmid pET15b/VP1 and expressed in E. coli BL21. The recombinant VP1 protein could specifically react with EV71-infected patient sera without the cross-reaction with serum antibodies of coxsackievirus A16 (CA16), A4, A5, B3 and B5 as well as echovirus 6. In acute and convalescent phases, IgM and IgG antibodies of 182 serum samples were detected by ELISA with recombinant VP1 protein as a coated antigen. The results showed that the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of IgM antibodies in serum samples for the diagnosis of EV71 infection were 90.1, 98.4, 98.8 and 88.7%, respectively; similarly, those of IgG antibodies in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterovirus 71; Gene cloning; Recombinant VPl protein; ELISA. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400027 Quantification of natural populations of Gluconacetobacter diazotrophicus and Herbaspirillum spp. In sugar cane (Saccharum spp.) Using differente polyclonal antibodies BJM Silva-Froufe,Lúcia Gracinda da; Boddey,Robert Michael; Reis,Veronica Massena. The species Gluconacetobacterdiazotrophicus, Herbaspirillum seropedicae and H. rubrisubalbicans are endophytic N2-fixing [diazotrophic] bacteria which colonise not only roots, but also the aerial tissue of sugar cane. However, the technique most commonly used to quantify the populations of these microbes in plants is by culturing serial dilutions of macerates of plant tissues in N free semi-solid media which are only semi-selective for the species/genera [the Most Probable Number (MPN) Technique] and each culture must be further subjected to several tests to identify the isolates at the species level. The use of species-specific polyclonal antibodies with the indirect ELISA (enzyme-linked immunosorbent assay) can be an alternative which is rapid and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Diazotrophic bacteria; ELISA; Immunoquantification. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400018 Analysis of isotype-specific antibody responses to bovine herpesviruses 1.1 and 1.2a allows to estimate the stage of infection BJM Spilki,Fernando Rosado; Franco,Ana Cláudia; Roehe,Paulo Michel. Specific IgM, IgA, IgG1, IgG2, as well as neutralizing antibody responses were evaluated in sera of calves experimentally infected with two isolates of bovine herpesvirus type 1 (BoHV1) of distinct subtypes (subtype 1, BoHV1.1; subtype 2a, BoHV-1.2a). No significant differences were observed in the antibody responses induced by each BoHV-1 subtype. The antibody responses following primary acute infection were characterized by an increase in specific IgM and IgA levels between days 2 and 14 post inoculation (pi). IgG1 was detected from days 11 to 30 pi. IgG2 was detected on the sample taken on day 30 pi. Reactivation of infection following dexamethasone administration induced a significant rise in IgA levels, whereas IgG1 and IgG2 levels, which were at high... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine herpesvirus type 1; Infectious bovine rhinotracheitis; Immunoglobulin subclasses; ELISA. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200021 An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum BJM Marassi,Carla Dray; Silva,Marley; Oelemann,Walter Martin Roland; Fonseca,Leila de Souza; Ristow,Paula; Lilenbaum,Walter. ELISAs for paratuberculosis employ a preadsorption step with Mycobacterium phlei to diminish unspecific reactions As M. fortuitum is one of the most frequent environmental mycobacteria, the purpose of this pilot study was to evaluate its use as an alternative for the preadsorption in ELISAs for paratuberculosis. Results suggest that M. fortuitum can be an alternative instead of or associated to M. phlei with comparable results (κ > 0.8) to conventional ELISAs using M. phlei as a preadsorption antigen. Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacteria; ELISA; M. fortuitum; M. phlei. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300019 MONOCLONAL ANTIBODIES TO IDENTIFY TOMATO MOSAIC TOBAMOVIRUS (TOMV) BJM Duarte,Keila M.R.; Gomes,Luiz Humberto; Gesztesi,Jean-Luc; Lopes,José Daniel; Tavares,Flávio C.A.. Monoclonal antibodies were obtained against Tomato mosaic tobamovirus (ToMV) isolated in Brazil. One antibody (8G7G2) isotyped as IgG2b (kappa light chain) showed strong specificity and very low cross reaction with the Tobacco mosaic virus (TMV). It can be used in identification of tomato mosaic virus (ToMV). Tipo: Info:eu-repo/semantics/other Palavras-chave: Tomato mosaic tobamovirus; ELISA; Monoclonal antibodies. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300016 Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans BJM Nakanishi,Fernanda Akemi; Avila-Campos,Mario Julio; Kamiji,Nádia Hizuru; Itano,Eiko Nakagawa. Actinobacillus actinomycetemcomitans produces a protease to human immunoglobulin G that is an important evasion mechanism. In this study, the proteolytic activity of A. actinomycetemcomitans strain ATCC 43718 on human immunoglobulin G associated with culture supernatant concentrations, the growth period and the period of incubation with immunoglobulin G were evaluated by an enzyme linked immunosorbent assay. The protease fraction was detected by Sephadex G 150 chromatography. The results showed that A. actinomycetemcomitans produced a protease to human immunoglobulin G in the culture supernatant, and the highest activity was achieved witen the concentration was 27.5 mug protein/mL, after culturing for 72 hours and incubating with IgG for 24 hours. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Immunoglobulin G; Actinobacillus actinomycetemcomitans; ELISA; Protease. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100008 Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat BJM Schneid,Andréa dos Santos; Rodrigues,Kelly Lameiro; Chemello,Davi; Tondo,Eduardo Cesar; Ayub,Marco Antônio Zacchia; Aleixo,José Antonio Guimarães. In this work, an indirect ELISA based on a monoclonal antibody (MAb) specific for an outer membrane protein of Salmonellaenterica serovar Enteritidis was used for detection of Salmonella in 154 samples of chicken meat. Its efficiency was determined through comparison with the results obtained from the conventional method. The prevalence of samples contaminated with Salmonella was 23% with the conventional culture method, and 26% with the ELISA. From thirty-five samples positive for Salmonella by the conventional method, 33 were also positive by ELISA. Seven other samples were only positive in the ELISA. Comparison of the results obtained in the two methods showed an ELISA sensitivity and specificity of 94%, and positive and negative predictive values of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Monoclonal antibody; Policlonal antibody; ELISA. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300027 Development of an indirect ELISA based on recombinant capsid protein to detect antibodies to bovine leukemia virus BJM Andreolla,Ana Paula; Erpen,Luana Marina Scheer; Frandoloso,Rafael; Kreutz,Luiz Carlos. Abstract Serological testing and culling infected animals are key management practices aiming eradication of bovine leukemia virus infection. Here, we report the development of an indirect ELISA based on BLV recombinant capsid protein (BLVp24r) to detect anti-BLV antibodies in cattle serum. The BLVp24r was expressed in Escherichia coli and purified by affinity chromatography, and then used to set up the ELISA parameters. The Polysorp ® plate coated with 50 ng of antigen/well and bovine serum diluted 1:100 gave the best results during standardization. Using sera from infected and non-infected cattle we set up the cutoff point at 0.320 (OD450 nm) with a sensitivity of 98.5% and specificity of 100.0%. Then, we tested 1.187 serum samples from dairy (736... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine leukemia virus; Retrovirus; Diagnosis; ELISA. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500068 Up-regulation of human immune system function by Donkey’s Milk BJPS Taghiloo,Saeid; Allahmoradi,Esmaeil; Sadeghian-Kiadehi,Seyedeh Forough; Omrani-Nava,Versa; Nazar,Eisa; Ebrahimzadeh,Mohammad Ali. Donkey’s milk represents a good alternative to human milk because of its chemical characteristics similar to those of human’s. In present study, the pro- and anti-inflammatory effects of donkey’s milk were evaluated on peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from 12 young and 12 aged normal subjects. PBMCs were cultured with or without the optimal and non-cytotoxic dose of pasteurized donkey’s milk, and polymyxin B was used to inhibit the possible endotoxin contamination. Following 18 hours incubation, culture supernatants were harvested to measure the secreted Tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6), Interleukin-8 (IL-8) and Interleukin-10 (IL-10) by ELISA. Donkey’s milk significantly increased TNF-α (p= 0.01), IL-8... Tipo: Info:eu-repo/semantics/article Palavras-chave: Donkey’s milk; ELISA; Immune system; Aged subject; Cytokine. Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100607 Ensaio imunoenzimático indireto (ELISA) para detecção de anticorpos anti-Rhodococcus equi em potros Ciência Rural Martins,Carla Braga; Bonesso,Maria Antonieta; Lima,Marcelo Mandrá; Ferraz,Luciana Colbachini; Lacerda Neto,José Corrêa de; Machado,Rosângela Zacarias. A infecção ocasionada pelo Rhodococcus equi é responsável por elevadas taxas de mortalidade e grandes perdas econômicas na equideocultura. Diante da importância dessa enfermidade, este trabalho objetivou a padronização de um ensaio imunoenzimático indireto (ELISA teste) para a detecção de anticorpos anti-Rhodococcus equi em potros (n=16) antes e após a ingestão de colostro de mães vacinadas. Nesse ensaio, foram utilizados dois tipos de antígenos de Rhodococcus equi, o APTX (proteína semi-purificada VapA, extraído pelo detergente Triton X e precipitado em acetona), e o comercial¹. Os resultados da titulação em bloco determinou a concentração ótima dos antígenos em 2,0µg/ml e a diluição única do soro 1:200 para soros de referências negativo, positivo e soros... Tipo: Info:eu-repo/semantics/article Palavras-chave: Potros; ELISA; Rhodococcus equi. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000300020 An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses Ciência Rural Baldani,Cristiane Divan; Machado,Rosangela Zacarias; Botteon,Paulo de Tarso Landgraf; Takakura,Felipe Santoro; Massard,Carlos Luiz. A crude antigenic preparation of Babesia equi was used to develop and establish the suitability of an enzyme-linked immunosorbent assay (ELISA) for the detection of parasite carriers. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkboard titrations. The specificity and sensitivity of the ELISA were 100 %. A total of 90 serum samples were taken from horses from the Northeast region of São Paulo State and examined for diagnosis of equine B. equi infection by ELISA. Approximately 75% (n=67) of all the horses tested were found serologically positive for B. equi. These results suggest that the ELISA described may prove to be an appropriate serological test for epidemiological studies on B. equi infections in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Babesia equi; Equine piroplasmosis; ELISA. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000500031 SEROLOGICAL SCREENING FOR INFECTIOUS CATTLE DISEASES: II. ASSOCIATION BETWEEN PREVALENCE AND LEVEL OF ELISA RESPONSE Ciência Rural Rojas,José Alfonso Barajas-; Riemann,Hans; Franti,Charles. A herd of cattle (Holstein-Zebu crosses) was screened every two months by ELISA during a period of two years for IgG antibodies against 19 infectious disease agents. Two hundred and ninety five sera were collected from 157 young animals (0-4 months of age), 1037 sera from 292 developing animals (4-36 months of age) and 1468 sera from 259 producing animals (> 36 months of age). The results indicate that the difference in ELISA between positive and negative tests is associated with the overall prevalence of positive tests. When the prevalence of positive tests is low the difference between positive and negative tests is greater than when the prevalence is intermediate or high. This means that ELISA, presumably other serological tests for IgG antibodies,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Serological screening; Cattie diseases; ELISA. Ano: 1993 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000200014 Monitoramento da presença de Mycoplasma hyopneumoniae em granjas de suínos durante a implementação de programas de erradicação Ciência Rural Tamiozzo,Pablo Jesús; Pelliza,Bibiana Rosa; Carranza,Alicia Isabel; Ambrogi,Arnaldo. O objetivo deste estudo foi monitorar a presença de M. hyopneumoniae em granjas suínas durante a implementação de programas de erradicação utilizando diferentes técnicas de diagnóstico focalizando no PCR. Trabalhou-se com uma empresa que possuía três granjas, uma parto-terminação (390 matrizes), uma múltiplo-sítio (4100 matrizes) e uma nova granja que povoava suas novas instalações. Nas duas primeiras, foi desenvolvido um programa de despovoamento parcial para erradicar a pneumonia enzoótica suína, a última foi povoada pelos suínos dos anteriores após a erradicação. Nos três rebanhos, os suínos foram monitorados por: sorologia (ELISA), PCR, lesões pulmonares macro e microscópicas e a presença de tosse não produtiva. A ausência de tosse, a baixa porcentagem... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoplasma hyopneumoniae; Pneumonia enzoótica suína (PES); NPCR; ELISA; Lesões pulmonares. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000400025 Elisa indireto na detecção de Salmonella spp. em lingüiça suína Ciência Rural Loguercio,Andrea Pinto; Aleixo,José Antonio Guimarães; Vargas,Agueda Castagna de; Costa,Mateus Matiuzzi da. Um teste ELISA, baseado em um anticorpo monoclonal (MAb) específico para uma proteína de membrana externa de Salmonella enterica serovar Enteritidis foi comparado com o método de cultivo tradicional na detecção de Salmonella spp. em 110 amostras de lingüiça suína frescal. A prevalência do patógeno nas amostras foi de 11,82% de acordo com o cultivo tradicional. O teste ELISA revelou sensibilidade, especificidade, valores preditivos positivos e negativos de 100%, 98%, 87% e 100%, respectivamente. Tais achados indicam que, comparado ao sistema tradicional, o teste imunológico foi bastante eficaz na determinação de Salmonella spp. em amostras de lingüiças naturalmente contaminadas. Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; ELISA; Lingüiça suína. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000600022 Leishmaniose visceral em um canino de Cruz Alta, Rio Grande do Sul, Brasil Ciência Rural Krauspenhar,Cristina; Beck,Cristiane; Sperotto,Vitor; Silva,Aline Alves da; Bastos,Rodrigo; Rodrigues,Lauro. Descreve-se um caso de leishmaniose visceral em um canino, macho, Akita, cinco anos, que foi atendido no Hospital Veterinário da Universidade de Cruz Alta, RS, após residir três anos no Estado de Alagoas. Clinicamente observou-se emagrecimento progressivo, lesões crostosas na ponta das orelhas, focinho e região periorbital, úlcera na região distal do membro torácico direito, opacidade da córnea, vômitos e eventuais episódios de diarréia. O exame sorológico para leishmaniose pelo método de ELISA resultou positivo. Na avaliação da medula óssea por imprints (corados por Panóptico), foi possível observar formas amastigotas no citoplasma de macrófagos. A histopatologia consistia de reação linfohistioplasmocitária acentuada, com estruturas basofílicas sugestivas... Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmaniose visceral; Leishmania sp.; Amastigotas; ELISA; Imunoistoquímica. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000300052 MARKOV CHAIN MODELING OF ENDEMIC CATTLE DISEASES IN THE TROPICS OF MEXICO Ciência Rural Rojas,José Alfonso Barajas; Riemann,Hans; Franti,Charles. Endemic diseases measured by prevalence of IgG antibodies by ELISA all showed remarkably stable overall prevalences over a study period of 3 years. This however does not mean that incidence is low or absent. Observed transition from the state of being positive for a disease to the state of being negative and vice versa suggested fairly high incidence rates (and rates of waning antibodies) for all 19 disease agents tested for. In spite of this dynamic situation Markov Chain simulations indicate that a steady prevalence is reached in a fairly short time. Markov Chain simulations may also be useful in elucidating seasonal changes in prevalence. Tipo: Info:eu-repo/semantics/article Palavras-chave: Prevalence; Markov Chain; México; ELISA. Ano: 1993 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000300014 Frequência de anticorpos e fatores de risco para a infecção pelo vírus da diarreia viral bovina em fêmeas bovinas leiteiras não vacinadas na região Amazônica Maranhense, Brasil Ciência Rural Chaves,Nancyleni Pinto; Bezerra,Danilo Cutrim; Sousa,Vanessa Evangelista de; Santos,Hamilton Pereira; Pereira,Hélder de Moraes. O presente estudo teve como objetivo determinar a frequência e os fatores de risco para a infecção pelo vírus da diarreia viral bovina (BVDV) em fêmeas bovinas leiteiras na região amazônica maranhense. Amostras de soro de animais não vacinados contra o BVDV e provenientes de 40 propriedades foram submetidas à técnica de ELISA indireto. Em cada propriedade avaliada, aplicou-se ainda um questionário epidemiológico para investigar os fatores de risco que poderiam estar associados à infecção pelo vírus. As amostras foram coletadas de animais que apresentavam ou não sinais clínicos sugestivos da infecção pelo BVDV. Das 400 amostras de soro analisadas, 61,5% (n=246) foram reagentes com a detecção de bovinos sorologicamente positivos em 95% (n=38) das... Tipo: Info:eu-repo/semantics/article Palavras-chave: BVDV; Anticorpos; ELISA; Vacas leiteiras; Fatores de risco. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000600035 Registros recuperados: 174 Primeira ... 1 2 3 4 5 6 7 8 9 ... Última

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