SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Brinn,L.S.; Pereira Leite,J.; Larson,R.E.; Martins,A.R..

Myosin Va is an actin-based, processive molecular motor protein highly enriched in the nervous tissue of vertebrates. It has been associated with processes of cellular motility, which include organelle transport and neurite outgrowth. The in vivo expression of myosin Va protein in the developing nervous system of mammals has not yet been reported. We describe here the immunolocalization of myosin Va in the developing rat hippocampus. Coronal sections of the embryonic and postnatal rat hippocampus were probed with an affinity-purified, polyclonal anti-myosin Va antibody. Myosin Va was localized in the cytoplasm of granule cells in the dentate gyrus and of pyramidal cells in Ammon's horn formation. Myosin Va expression changed during development, being...

Effects of prenatal exposure to a mild chronic variable stress on body weight, preweaning mortality and rat behavior

Palavras-chave: Myosin Va; Growth and development; Central nervous system; Hippocampus; Rat; Immunohistochemistry.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900012

Cabrera,R.J.; Rodríguez-Echandía,E.L.; Jatuff,A.S.G.; Fóscolo,M..

Early stimulation has been shown to produce long-lasting effects in many species. Prenatal exposure to some strong stressors may affect development of the nervous system leading to behavioral impairment in adult life. The purpose of the present work was to study the postnatal harmful effects of exposure to variable mild stresses in rats during pregnancy. Female Holtzman rats were submitted daily to one session of a chronic variable stress (CVS) during pregnancy (prenatal stress; PS group). Control pregnant rats (C group) were undisturbed. The pups of PS and C dams were weighed and separated into two groups 48 h after delivery. One group was maintained with their own dams (PS group, N = 70; C group, N = 36) while the other PS pups were cross-fostered with C...

Respiratory mechanics and morphometric changes during anesthesia with ketamine in normal rats

Palavras-chave: Prenatal stress; Chronic stress; Body weight; Behavior; Mortality; Rat.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000009

Alves-Neto,O.; Tavares,P.; Rocco,P.R.M.; Zin,W.A..

Ketamine is believed to reduce airway and pulmonary tissue resistance. The aim of the present study was to determine the effects of ketamine on the resistive, elastic and viscoelastic/inhomogeneous mechanical properties of the respiratory system, lungs and chest wall, and to relate the mechanical data to findings from histological lung analysis in normal animals. Fifteen adult male Wistar rats were assigned randomly to two groups: control (N = 7) and ketamine (N = 8). All animals were sedated (diazepam, 5 mg, ip) and anesthetized with pentobarbital sodium (20 mg/kg, ip) or ketamine (30 mg/kg, ip). The rats were paralyzed and ventilated mechanically. Ketamine increased lung viscoelastic/inhomogeneous pressure (26%) compared to the control group. Dynamic and...

Respiratory effects of kynurenic acid microinjected into the ventromedullary surface of the rat

Palavras-chave: Ketamine; Elastance; Lung tissue; Resistive pressure; Viscoelasticity; Rat.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900016

Tolentino-Silva,F.P.; Russo,A.K.; Cravo,S.L.; Lopes,O.U..

Several studies demonstrate that, within the ventral medullary surface (VMS), excitatory amino acids are necessary components of the neural circuits involved in the tonic and reflex control of respiration and circulation. In the present study we investigated the cardiorespiratory effects of unilateral microinjections of the broad spectrum glutamate antagonist kynurenic acid (2 nmol/200 nl) along the VMS of urethane-anesthetized rats. Within the VMS only one region was responsive to this drug. This area includes most of the intermediate respiratory area, partially overlapping the rostral ventrolateral medulla (IA/RVL). When microinjected into the IA/RVL, kynurenic acid produced a respiratory depression, without changes in mean arterial pressure or heart...

Pituitary-thyroid axis in short- and long-term experimental diabetes mellitus

Palavras-chave: Ventral surface of the medulla; Breathing pattern; Cardiorespiratory control; Kynurenic acid; Rat.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000016

Short-term experimental diabetes mellitus (DM) produces a significant decrease in serum thyroid hormones, a decreased or normal serum thyroid-stimulating hormone (TSH) and a reduction in hepatic and renal T4-5'-deiodination. However, little is known about the effects of chronic diabetes mellitus on the pituitary-thyroid axis function. We evaluated the changes induced by very short-term (6 days), short-term (15 days) and chronic (6 months) streptozotocin-induced diabetes mellitus in 3-month old female Dutch-Miranda rat serum T4, serum TSH and T4-5'-deiodinase activity in the thyroid and pituitary glands. Serum hormones were determined by specific radioimmunoassays. Iodothyronine-5'-deiodinase activities were assayed in the thyroid and pituitary microsomal...

Biodistribution of 99m technetium- labeled creatinine in healthy rats

Palavras-chave: Thyroid; Pituitary; Diabetes mellitus; T4-5'-deiodinase; Serum T4; Serum TSH; Rat; Streptozotocin.

Ano: 1997

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200017

Yilmaz,O.; Yurt Lambrecht,F.; Soylu,A.; Durkan,K.; Kavukcu,S..

The distribution of creatinine, one of the toxic guanidine compounds, in various tissues has not been studied in detail by using radiolabeled creatinine. Our objective was to investigate the biodistribution of creatinine labeled with 99m technetium (99mTc) by the stannous (II) chloride method in healthy male Wistar rats. Quality controls were carried out by radio thin layer chromatography, high-performance liquid chromatography, and paper electrophoresis. The labeling yield was 85 ± 2% under optimum conditions (pH 7 and 100 µg stannous chloride). Rats (N = 12) were injected intravenously with 99mTc-creatinine and their blood and visceral organs were evaluated for 99mTc-creatinine uptake as percent of the injected dose per gram wet weight of each tissue...

Autoradiographic thyroid evaluation in short-term experimental diabetes mellitus

Palavras-chave: Biodistribution; Creatinine; 99mTechnetium; 99mTc-creatinine; Rat.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600009

Nascimento-Saba,C.C.A.; Brito,A.C.; Pereira,M.J.S.; Carvalho,J.J.; Rosenthal,D..

Previous studies have shown that in vitro thyroid peroxidase (TPO) iodide oxidation activity is decreased and thyroid T4-5'-deiodinase activity is increased 15 days after induction of experimental diabetes mellitus (DM). In the present study we used thyroid histoautoradiography, an indirect assay of in vivo TPO activity, to determine the possible parallelism between the in vitro and in vivo changes induced by experimental DM. DM was induced in male Wistar rats (about 250 g body weight) by a single ip streptozotocin injection (45 mg/kg), while control (C) animals received a single injection of the vehicle. Seven and 30 days after diabetes induction, each diabetic and control animal was given ip a tracer dose of 125I (2 µCi), 2.5 h before thyroid excision....

Role of the caudal pressor area in the regulation of sympathetic vasomotor tone

Palavras-chave: Thyroid; Diabetes mellitus; Autohistoradiography; Rat.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200013

Campos,R.R.; Carillo,B.A.; Oliveira-Sales,E.B.; Silva,A.M.; Silva,N.F.; Futuro Neto,H.A.; Bergamaschi,C.T..

It is well known that the ventrolateral medulla contains neurons involved in the tonic and reflex control of the cardiovascular system. Two regions within the ventrolateral medulla were initially identified: the rostral ventrolateral medulla (RVLM) and the caudal ventrolateral medulla (CVLM). Activation of the RVLM raises arterial blood pressure and sympathetic nerve activity, and activation of the CVLM causes opposite effects. The RVLM premotor neurons project directly to sympathetic preganglionic neurons and are involved in the maintenance of resting sympathetic vasomotor tone. A significant proportion of tonic activity in the RVLM sympathetic premotor neurons is driven by neurons located in a third region of the ventrolateral medulla denominated caudal...

Long-term effects of human amniotic membrane in a rat model of biliary fibrosis

Palavras-chave: Rostral ventrolateral medulla; Rat; Glutamate; Blood pressure; Caudal pressor area; Sympathetic vasomotor tone.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700002

Sant'Anna,L.B.; Brito,F.S.; Barja,P.R.; Nicodemo,M.C..

Liver fibrosis is the most common outcome of chronic liver diseases, and its progression to cirrhosis can only be effectively treated with liver transplantation. The amniotic membrane (AM) has been studied as an alternative therapy for fibrosis diseases mainly for its favorable properties, including anti-inflammatory, anti-scaring and immunomodulatory properties. It was recently demonstrated that the AM reduces the progression of biliary fibrosis to its advanced stage, cirrhosis, when applied on the liver for 6 weeks after fibrosis induction. Here, we investigated the effects of AM on rat fibrotic liver, during a prolonged period of time. Fibrosis was induced by bile duct ligation (BDL), and at the same time, a fragment of AM was applied around the liver....

Cardiac function and intracellular Ca2+ handling proteins are not impaired by high-saturated-fat diet-induced obesity

Palavras-chave: Amniotic membrane; Bile duct ligation; Liver fibrosis; Long-term; Quantitative image analysis; Rat.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700611

Deus,A.F.; Vileigas,D.F.; Silva,D.C.T.; Tomasi,L.C.; Campos,D.H.S.; Okoshi,K.; Padovani,C.R.; Cicogna,A.C..

Obesity is often associated with changes in cardiac function; however, the mechanisms responsible for functional abnormalities have not yet been fully clarified. Considering the lack of information regarding high-saturated-fat diet-induced obesity, heart function, and the proteins involved in myocardial calcium (Ca2+) handling, the aim of this study was to test the hypothesis that this dietary model of obesity leads to cardiac dysfunction resulting from alterations in the regulatory proteins of intracellular Ca2+ homeostasis. Male Wistar rats were distributed into two groups: control (C, n=18; standard diet) and obese (Ob, n=19; high-saturated-fat diet), which were fed for 33 weeks. Cardiac structure and function were evaluated using echocardiographic and...

Evidence for eosinophil recruitment, leukotriene B4 production and mast cell hyperplasia following Toxocara canis infection in rats

Palavras-chave: Obesity; Saturated fatty acids; High-fat diet; Cardiac function; Calcium handling proteins; Rat.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600605

Carlos,D.; Machado,E.R.; De Paula,L.; Sá-Nunes,A.; Sorgi,C.A.; Jamur,M.C.; Oliver,C.; Lima,W.T.; Faccioli,L.H..

It is well known that eosinophilia is a key pathogenetic component of toxocariasis. The objective of the present study was to determine if there is an association between peritoneal and blood eosinophil influx, mast cell hyperplasia and leukotriene B4 (LTB4) production after Toxocara canis infection. Oral inoculation of 56-day-old Wistar rats (N = 5-7 per group) with 1000 embryonated eggs containing third-stage (L3) T. canis larvae led to a robust accumulation of total leukocytes in blood beginning on day 3 and peaking on day 18, mainly characterized by eosinophils and accompanied by higher serum LTB4 levels. At that time, we also noted increased eosinophil numbers in the peritoneal cavity. In addition, we observed increased peritoneal mast cell number in...

The compulsive-like aspect of the head dipping emission in rats with chronic electrolytic lesion in the area of the median raphe nucleus

Palavras-chave: Mast cells; Leukotriene B4; Eosinophils; Eosinophil peroxidase; Toxocara canis; Rat.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400008

Hoshino,K.; Uga,D.A.; de Paula,H.M.G..

Head dipping (HD) is a behavioral pattern considered to have a risk assessment or an exploratory role and is used as a complementary parameter to evaluate anxiety in experimental animals. Since rats with electrolytic lesion in the area of the median raphe nucleus displayed high frequencies of HD in a previous study, the present investigation was undertaken to confirm this observation and to determine its anxiety-related origin. HD episodes were counted in adult male Wistar rats (270-350 g) with electrolytic lesion (N = 11) and sham-lesioned controls (N = 12). When HD was measured for 60 min on an elevated open platform, lesioned rats emitted 13 times more HD than controls (264.7 ± 93.3 vs 20.3 ± 7.6 episodes), with the difference being statistically...

Effect of hyperbaric oxygenation on mitochondrial function of neuronal cells in the cortex of neonatal rats after hypoxic-ischemic brain damage

Palavras-chave: Head dipping; Repetitive behavior; Median raphe nucleus; Electrolytic lesion; Rat.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200012

Yang,L.; Hei,M.Y.; Dai,J.J.; Hu,N.; Xiang,X.Y..

The timing and mechanisms of protection by hyperbaric oxygenation (HBO) in hypoxic-ischemic brain damage (HIBD) have only been partially elucidated. We monitored the effect of HBO on the mitochondrial function of neuronal cells in the cerebral cortex of neonatal rats after HIBD. Neonatal Sprague-Dawley rats (total of 360 of both genders) were randomly divided into normal control, HIBD, and HIBD+HBO groups. The HBO treatment began immediately after hypoxia-ischemia (HI) and continued once a day for 7 consecutive days. Animals were euthanized 0, 2, 4, 6, and 12 h post-HI to monitor the changes in mitochondrial membrane potential (ΔΨm) occurring soon after a single dose of HBO treatment, as well as 2, 3, 4, 5, 6, and 7 days post-HI to study ΔΨm changes after...

Effect of lead acetate on neurobehavioral development of rats

Palavras-chave: Hyperbaric oxygenation; Hypoxia-ischemia; Brain; Mitochondrial function; Neonatal; Rat.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500704

Mello,C.F.; Kraemer,C.K.; Filippin,A.; Morsch,V.M.; Rodrigues,A.L.S.; Martins,A.F.; Rubin,M.A..

We investigated the effects of lead exposure during the pre- and postnatal period on the neurobehavioral development of female Wistar rats (70-75 days of age, 120-150 g) using a protocol of lead intoxication that does not affect weight gain. Wistar rats were submitted to lead acetate intoxication by giving their dams 1.0 mM lead acetate. Control dams received deionized water. Growth and neuromotor development were assessed by monitoring daily the following parameters in 20 litters: body weight, ear unfolding, incisor eruption, eye opening, righting, palmar grasp, negative geotaxis, cliff avoidance and startle reflex. Spontaneous alternation was assessed on postnatal day 17 using a T maze. The animals' ability to equilibrate on a beaker rim was measured on...

Differential role of entorhinal and hippocampal nerve growth factor in short- and long-term memory modulation

Palavras-chave: Lead; Neurobehavioral development; Undernutrition; Heavy metal intoxication; Teratology; Rat.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700010

Walz,R.; Roesler,R.; Reinke,A.; Martins,M.R.; Quevedo,J.; Izquierdo,I..

We studied the effects of infusion of nerve growth factor (NGF) into the hippocampus and entorhinal cortex of male Wistar rats (250-300 g, N = 11-13 per group) on inhibitory avoidance retention. In order to evaluate the modulation of entorhinal and hippocampal NGF in short- and long-term memory, animals were implanted with cannulae in the CA1 area of the dorsal hippocampus or entorhinal cortex and trained in one-trial step-down inhibitory avoidance (foot shock, 0.4 mA). Retention tests were carried out 1.5 h or 24 h after training to measure short- and long-term memory, respectively. Immediately after training, rats received 5 µl NGF (0.05, 0.5 or 5.0 ng) or saline per side into the CA1 area and entorhinal cortex. The correct position of the cannulae was...

Role of nitric oxide in hypoxia-induced hyperventilation and hypothermia: participation of the locus coeruleus

Palavras-chave: Nerve growth factor; Hippocampus; Entorhinal cortex; Short- and long-term memory; Inhibitory avoidance; Rat.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100009

Fabris,G.; Anselmo-Franci,J.A.; Branco,L.G.S..

Hypoxia elicits hyperventilation and hypothermia, but the mechanisms involved are not well understood. The nitric oxide (NO) pathway is involved in hypoxia-induced hypothermia and hyperventilation, and works as a neuromodulator in the central nervous system, including the locus coeruleus (LC), which is a noradrenergic nucleus in the pons. The LC plays a role in a number of stress-induced responses, but its participation in the control of breathing and thermoregulation is unclear. Thus, in the present study, we tested the hypothesis that LC plays a role in the hypoxia-induced hypothermia and hyperventilation, and that NO is involved in these responses. Electrolytic lesions were performed bilaterally within the LC in awake unrestrained adult male Wistar rats...

An ultrasound and histomorphological analysis of experimental liver cirrhosis in rats

Palavras-chave: Nitric oxide; Locus coeruleus; Hypoxia; Ventilation; Body temperature; Rat.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100009

Dias,J.V.; Paredes,B.D.; Mesquita,L.F.Q.; Carvalho,A.B.; Kozlowski,E.O.; Lessa,A.S.; Takiya,C.M.; Resende,C.M.C.; Coelho,H.S.M.; Campos-de-Carvalho,A.C.; Rezende,G.F.M.; Goldenberg,R.C.S..

We investigated whether liver injury by dual exposure to ethanol and carbon tetrachloride (EtOH + CCl4) for 15 weeks would persist after hepatotoxic agents were removed (EtOH + CCl4/8wR). After 15 weeks of hepatic injury with ethanol (5.5%, m/v) and carbon tetrachloride (0.05, mL/kg, ip), 5 of 11 female Wistar rats were sacrificed. The other 6 rats were maintained for an additional 8 weeks without hepatotoxic agents. Ultrasonography showed increased liver echogenicity and dilation of portal vein caliber in both groups (EtOH + CCl4: 0.22 ± 0.01 cm, P < 0.001; EtOH + CCl4/8wR: 0.21 ± 0.02 cm, P < 0.01) vs control (0.16 ± 0.02 cm). Histopathology showed regenerative nodules in both experimental groups. Histomorphometry revealed increased fibrosis...

Regenerated rat skeletal muscle after periodic contusions

Palavras-chave: Rat; Cirrhosis; Collagen; Ultrasound; CCl4; Ethanol.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100008

Minamoto,V.B.; Bunho,S.R.; Salvini,T.F..

In the present study we evaluated the morphological aspect and changes in the area and incidence of muscle fiber types of long-term regenerated rat tibialis anterior (TA) muscle previously submitted to periodic contusions. Animals received eight consecutive traumas: one trauma per week, for eight weeks, and were evaluated one (N = 8) and four (N = 9) months after the last contusion. Serial cross-sections were evaluated by toluidine blue staining, acid phosphatase and myosin ATPase reactions. The weight of injured muscles was decreased compared to the contralateral intact one (one month: 0.77 ± 0.15 vs 0.91 ± 0.09 g, P = 0.03; four months: 0.79 ± 0.14 vs 1.02 ± 0.07 g, P = 0.0007, respectively) and showed abundant presence of split fibers and fibers with...

Screening and characterization of lactic acid bacterial strains that produce fermented milk and reduce cholesterol levels

Palavras-chave: Skeletal muscle; Periodic contusion; Long-term regeneration; Rat.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100012

BJM

Guan,Xuefang; Xu,Qingxian; Zheng,Yi; Qian,Lei; Lin,Bin.

ABSTRACT Objective To screen for and characterize lactic acid bacteria strains with the ability to produce fermented milk and reduce cholesterol levels. Methods The strains were isolated from traditional fermented milk in China. In vitro and in vivo evaluation of cholesterol-reduction were used to identify and verify strains of interest. Characteristics were analyzed using spectrophotometry and plate counting assays. Results The isolate HLX37 consistently produced fermented milk with strong cholesterol-reducing properties was identified as Lactobacillus plantarum (accession number: KR105940) and was thus selected for further study. The cholesterol reduction by strain HLX37 was 45.84%. The isolates were acid-tolerant at pH 2.5 and bile-tolerant at 0.5%...

Costus root aqueous extract modulates rat liver toxicity, DNA damage, injury, proliferation alterations induced by plant growth regulator Ethephon

Palavras-chave: Fermented milk; Lactobacillus plantarum; Cholesterol; Rat.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400730

BJPS

Tousson,Ehab; El-Atrsh,Afaf; Mansour,Merfaat; Assem,Abdallah.

Ethephon (Ethrel®) is an ethylene-based plant growth regulator that used in agriculture and it has direct and indirect effects on human health, direct effect via its inhalation during usage in agriculture and indirect effect through the diet (Fruits and vegetables) that is sprayed with it. The current study aimed to examine the possible modifying effects of costus (Saussurea lappa) root aqueous extract against Ethephon induced liver toxicity, injury, DNA fragmentation and PCNA alterations in male rats. Fifty adult male rats were divided into 5 groups (1st, control; 2nd, Costus; 3rd, Ethephon; 4th, Post treated Ethephon with costus; 5th, self-healing Ethephon). Current results revealed that; a significant increase in aspartate transaminase (AST), alanine...