|
|
|
| Registros recuperados: 181 | |
|
| |
|
|
| Di Mascio,P.; Dewez,B.; Garcia,C.R.S.. |
| We have studied the effect of peroxynitrite (ONOO-) on the membrane cytoskeleton of red blood cells and its protection by melatonin. Analysis of the protein fraction of the preparation by SDS-PAGE revealed a dose-dependent (0-600 µM ONOO-) disappearance at pH 7.4 of the main proteins: spectrin, band 3, and actin, with the concomitant formation of high-molecular weight aggregates resistant to reduction by ß-mercaptoethanol (2%) at room temperature for 20 min. These aggregates were not solubilized by 8 M urea. Incubation of the membrane cytoskeleton with ONOO- was characterized by a marked depletion of free sulfhydryl groups (50% at 250 µM ONOO-). However, a lack of effect of ß-mercaptoethanol suggests that, under our conditions, aggregate formation is not... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Peroxynitrite; Melatonin; Spectrin; Protein; Ghost; Thiols. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100002 |
| |
|
|
| Marchini,J.S.; Lambertini,C.R.; Ferriolli,E.; Dutra de Oliveira,J.E.. |
| This study examined if leucine, arginine or glycine supplementation in adult obese patients (body mass index of 33 ± 4 kg/m²) consuming a Brazilian low energy and protein diet (4.2 MJ/day and 0.6 g protein/kg) affects protein and amino acid metabolism. After four weeks adaptation to this diet, each subject received supplements of these amino acids (equivalent to 0.2 g protein kg-1 day-1) in random order. On the seventh day of each amino acid supplementation, a single-dose 15N-glycine study was carried out. There were no significant differences in protein flux, synthesis or breakdown. The protein flux (grams of nitrogen, gN/9 h) was 55 ± 24 during the nonsupplemented diet intake and 39 ± 10, 44 ± 22 and 58 ± 35 during the leucine-, glycine- and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Obese women; Protein; Glycine; Arginine; Leucine; Stable isotope. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000007 |
| |
|
|
| Bellato,Cláudia de M.; Garcia,Andréia K.M.; Mestrinelli,Fabiana; Tsai,Siu M.; Machado,Marcos A.; Meinhardt,Lyndel W.. |
| An in vitro system was developed to induce and identify Xylella fastidiosa proteins that were differentially expressed in the presence of callus-derived extracts from its host, the citrus cultivar Pêra. To optimize the induction, we first developed a single culture medium for the growth of both, host and bacteria. This medium, CPXPm7, which mimics the citrus xylem sap, showed that X. fastidiosa at 72 h post-incubation had 10(8) colony forming units mL-1, while Pêra cells had the highest fresh weight content (0.79 g). After testing various methods of co-cultivation of the bacteria and host callus grown in this single medium, the best induction procedure was to grow X. fastidiosa in a solid medium amended with an extract of Pêra callus grown in CPXPm7.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2DE; Callus; Pathogenicity; Protein; Xylella-citrus interaction. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200012 |
| |
|
|
| Coelho,Cileide Maria Medeiros; Santos,Júlio Cesar Pires; Tsai,Siu Mui; Vitorello,Victor Alexandre. |
| Phytic acid is the major storage form of phosphorus in seeds of legumes and cereals. Since phytate can form complexes with proteins and minerals, reducing the digestive availability of these nutrients, it is usually regarded as an antinutrient, although recent work indicates that it has important beneficial roles as an antioxidant and anticarcinogen. Therefore, there is an interest in the assessment and manipulation of phytate contents in important food grains such as beans. The objective of this work was to compare eleven dry bean genotypes with regard to grain contents of P, phytate, inorganic P and protein and to examine if differences between genotypes could be explained by differences in grain protein content or in uptake and partitioning of P. Plants... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inorganic phosphorus; Phaseolus; Phytic acid; Protein. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000100007 |
| |
|
|
| Miranda,Margarita; Vega-Gálvez,Antonio; Martínez,Enrique A; López,Jéssica; Marín,Rosa; Aranda,Mario; Fuentes,Francisco. |
| Quinoa (Chenopodium quinoa Willd.) in Chile represents a source of germplasm with high nutritional value. However, there is little information available related to quinoa seed quality grown under contrasting environments. In this study we evaluated the changes on seed composition of seeds of two lowland/coastal quinoa genotypes grown under arid (Vicuna) and cold-temperate (Temuco) conditions in Chile. Results showed that in the case of 'Regalona Baer' and 'Villarrica' genotypes the arid location (with irrigation) led to a significant increase (P < 0.05) in grain yield (4.2 and 5.1 t ha-1, respectively), soluble dietary fiber (16.8 ± 0.4 and 28.9 ± 2.1 g kg-1 DM, respectively), vitamin B3 (2.44 ± 0.005 and 2.26 ± 0.04 mg 100 g-1 DM, respectively),... |
| Tipo: Journal article |
Palavras-chave: Chenopodium quinoa; Climate stress; Antioxidant activity; Phenolic compounds; Protein; Mineral content. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392013000200004 |
| |
|
|
| Puga,Aline P; de Mello Prado,Renato; Mattiuz,Ben-Hur; Wyllyam do Vale,Diego; Fonseca,Ivana M. |
| In general, tropical soils present low concentrations of zinc (Zn), and the deficiency of Zn is recognized as a world nutritional problem for cereal production and human beings. Therefore, the main goal of this study was to assess the effects of different methods of Zn application on the quality of corn and sorghum grains grown in Oxisol. Two experiments were set up in the experimental area of UNESP (campus of Jaboticabal, Brazil). The following nine treatments were applied: three doses of Zn by banded application (seed furrows), three doses of Zn by incorporation into soil (0-20 cm depth), foliar application, seed application, and control (no Zn applied). The treatments were arranged in randomized blocks with four replicates. The contents of Zn,... |
| Tipo: Journal article |
Palavras-chave: Carbohydrate; Protein; Sorghum bicolor; Zea mays L; Zn. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000100008 |
| |
|
|
| Pablo-Pérez,Maricela; Lagunes-Espinoza,Luz del C; Ramos-Juárez,Jesús; López-Upton,Javier; Aranda-Ibáñez,Emilio M; Vargas-Villamil,Luis. |
| Ruminal degradation of dry matter (DMD) and crude protein (CPD) for aerial biomass at the flowering stage and for the seeds of L. campestris, L. exaltatus, L. hintonii and L. montanus was determined using the nylon bag technique with two fistulated steers in five incubation times (3, 9, 12, 24 and 48 h), including a sample of soybean paste (SP). The data obtained were analyzed using PROC MIXED of SAS for repeated measures. The results showed interactions between species and incubation time for DMD and CPD. Among species of lupine, a high DMD and CPD of seeds were observed after three hours of incubation. At 48 h, DMD and CPD did not show significant variation between species: DMD ranged from 94.3% to 96.7% and CPD from 98.9 to 99.2%. The degradation rate... |
| Tipo: Journal article |
Palavras-chave: Dry matter; Protein; Ruminal incubation; Legume; Lupinus. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202014000100001 |
| |
|
| |
|
|
| Coelho,Silvia R. M; Prudencio,Sandra H; Christ,Divair; Sampaio,Silvio C; Schoeninger,Vanderleia. |
| S.R.M. Coelho, S.H. Prudencio, D. Christ, S.C. Sampaio, and V. Schoeninger. 2013. Physico-chemical characterization of common beans under natural and accelerated storage conditions. Cien. Inv. Agr. 40(3): 629-636. The common bean is an important food in the human diet. Certain storage conditions, high temperatures and high humidity, cause a texture defect that reduces the acceptability of common beans. The present study aimed at evaluating the effect of storage on the physicochemical properties of common bean grains and the relationship between accelerated aging at 40 °C and 76% RH and storage under natural conditions. Two varieties of common beans (Iapar 81 - carioca variety and Iapar 44 - black variety) were stored at 40 °C and 76% RH for 15, 30, 45, 60... |
| Tipo: Journal article |
Palavras-chave: Food technology; Cooking time; Phaseolus vulgaris; Protein. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000300015 |
| |
|
|
| Rodrigues,Josiane Isabela da Silva; Arruda,Klever Márcio Antunes; Cruz,Cosme Damião; Barros,Everaldo Gonçalves de; Piovesan,Newton Deniz; Moreira,Maurilio Alves. |
| ABSTRACT: The objective of this paper was to estimate the genetic divergence among 49 soybean ( Glycine max L. Merrill .) genotypes to assist grain quality-focused breeding programs in the choice of progenitors. The genetic divergence was estimated using the Mahalanobis generalized distance from the percentages of protein, oil, and fatty acids oleic, linoleic and linolenic after cultivation of genotypes in different environments. Genotypes were grouped by agglomerative methods and the two and three-dimensional projections of the distance matrix were obtained. The average protein and oil contents in the four environments ranged from 34.25 to 45.18% and from 16.48 to 23.01%, respectively. The average contents of the fatty acids oleic, linoleic and linolenic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max L. Merrill.; Protein; Oil; Fatty acids.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000200402 |
| |
|
|
| Vargas,Diego Prado de; Nörnberg,José Laerte; Mello,Renius de Oliveira; Scheibler,Rudolf Brand; Mello,Fernanda Cristina Breda; Rizzo,Fábio Antunes; Bellato,Tatiana Kátia Bertussi. |
| ABSTRACT: Milk supply chain in Brazil exhibits significant production system heterogeneity in all federal units. Thus, the objective of this study was to form homogeneous groups of bovine milk production units based on the chemical and microbiological quality of the milk via multivariate statistical techniques. A total of 1,541 milk producing units (MPUs), corresponding to 44,089 samples, were analyzed. The first three principal components accounted for 81.38% of the total variation in the data. Principal component 1 (PC1) was associated with the chemical quality of milk (fat, protein [PROT] and total dry extract [TDE] content), while PC2 and PC3 were associated with microbiological quality (somatic cell count [SCC] and total bacterial count [TBC]). The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fat; Lactose; Protein; Total dry extract; Typology. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000400653 |
| |
|
|
| Alves,Marilia; González,Félix; Carvalho,Nelcy; Mühlbach,Paulo; Lima,Verônica; Conceição,Tiago Reis; Wald,Vera. |
| Soybean by-products are currently used in Rio Grande do Sul, Brazil, as a source of protein in dairy cows. However, the high protein breakdown of this feed source in the rumen causes loss of the intrinsic quality of the protein and increases plasma urea with deleterious consequences to animal metabolism. Thermal treatment of soybean can overcome this limitation. The aim of this study was to evaluate the metabolism of dairy cows fed with thermally treated soybean and raw soybean, through the analyses of the metabolic profile of plasma and milk. Twelve Holstein cows in mid-lactation period were studied using four treatments: commercial concentrated of protein, soybean meal, raw soybean and roasted soybean. Protein and urea levels in milk were measured in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metabolic profile; Roasted soybean; Dairy cows; Milk; Protein; Urea. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000100037 |
| |
|
|
| Manske,Guilherme Augusto; Rigo,Elisandra; Gomes,Fábio José; Schogor,Ana Luiza Bachmann. |
| ABSTRACT: Constant monitoring of milk quality by the processing industries demands the use of methodologies that add agility and low-cost analysis, such as the use of electronic equipment based on infrared or ultrasonic spectroscopy. In the present study, quality of raw milk received by dairy farms from western region of Santa Catarina state, Brazil, was evaluated throughout two methodologies of analysis of chemical composition and compared, being that both equipment used standard procedures for calibration, considering as reference data from the classic analytical methods recommended in Brazilian legislation. Milk samples from 45 producers were analyzed for SCC, TBC and physicochemical analysis, performed at dairy’s laboratory and at the official... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Composition; Fast methods; Fat; Milk quality; Protein. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000700451 |
| |
|
|
| Rosa,Paulo Roberto Antunes da; Bohrer,Rodrigo Camponogara; Ludke,Charles Alencar; Cesaro,Matheus Pedroti De; Pereira,Gabriel Ribas; Mondadori,Rafael Gianela; Antoniazzi,Alfredo Quites; Gonçalves,Paulo Bayard Dias. |
| The objective of this study was to investigate the mRNA expression and protein localization of Grb10 gene in bovine cumulus-oocyte complexes (COCs) from different follicle sizes. Firstly, it was investigated the mRNA expression to correlate with maturation rates. COCs from follicles at 1-3, 4-6, 6-8 and >8mm were used to evaluate Grb10 gene expression by qRT-PCR assay and nuclear maturation rates. It was observed that more competent oocytes (from follicles at 6-8 and >8mm; P>0.05), had lower Grb10 mRNA expression levels when compared to the oocytes from follicles at 1-3 and 4-6mm (P>0.05). After it was performed an immunofluorescence analysis in COCs from different follicle sizes (1-3, 4-6, 6-8 and >8mm) to investigate... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: : mRNA; Protein; Maturation; Oocyte; Grb10.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500898 |
| |
|
|
| Melo,Waldjânio de Oliveira; Monteiro,Bruno Moura; Chaves,Luciara Celi da Silva; Santos,Eduardo Riodades Daher; Souza,Damazio Campos de; Amorim,Bianca Souza de; Faria Junior,Sebastião Pereira de; Ribeiro Filho,José Dantas; Faturi,Cristian; Viana,Rinaldo Batista. |
| ABSTRACT: This study aimed to use ultrasound spectroscopy for the determination of the physical-chemical characteristics of buffalo milk and compare it to theinfrared method. Levels of fat, protein, lactose and non-fat solids (NFS) were determined in milk samples of 22 buffaloes (n = 383) with initial milk production of 6.97 ± 1.55 litres. The respective average results for the fat, protein, lactose and NFS of the individual samples were 6.31%, 3.81%, 4.99% and 9.75% for the infrared method-PO ANA 009 and 7.16%, 2.5%, 6.28% and 9.41% using ultrasound spectroscopy. There were significant differences (P<0.0001) in the levels of all of the components analysed between the two methods studied. Results obtained in the analyses using the ultrasonic milk... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Buffaloes; Milk composition; Fat; Protein; Infrared. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200753 |
| |
|
|
| Rego,Fabíola Cristine de Almeida; Belan,Laís; Pertile,Simone Fernanda Nedel; Lima,Lisiane Dorneles de; Ludovico,Agostinho; Zundt,Marilice; Lopes,Flávio Guiselli; Porto,Petrônio Pinheiro. |
| ABSTRACT: This study aimed to evaluate the effects of replacing oat hay with coffee husk in the diet of lambs on the intake and apparent digestibility of dry matter (DM), organic matter (OM), crude protein (CP), ether extract (EE), non-fiber carbohydrate (NFC), neutral detergent fiber (NDF), and acid detergent fiber (ADF). The experimental design was 4×4 Latin square with four levels of replacement of oat hay with coffee husk (0, 7.5, 15, and 22.5%) with four individually housed Texel sheep. Intake and apparent digestibility coefficients were measured at four periods of 12 days. All orts and feces were collected, weighed, homogenized, and sampled. The use of coffee husk at up to 22.5% of the total DM in the diet of lambs improved the intake of nutrients.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Byproduct; Dry matter; Fiber; Protein; Sheep. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000100655 |
| |
|
| |
|
|
| Sant'Ana,Rita de Cássia Oliveira; Mendes,Fabrícia Queiroz; Pires,Christiano Vieira; Oliveira,Maria Goreti de Almeida. |
| Proteins are the most abundant macromolecules in living cells and their primary role in the diet is to supply the body with essential amino acids in adequate quantities for the synthesis and maintenance of body tissues. The determination of protein digestibility of foods is an important factor to estimate their quality and the in vitro methodology is a fast and easy way to perform it. This study aimed to determine the influence of lipids on the in vitro digestibility of animal and vegetable proteins. The following protein sources: oat, beef, chicken, fish and pork meats, red beans, milk powder, textured soy protein (TSP), quinoa and five soybean varieties were evaluated. Animal proteins presented higher in vitro values than vegetable proteins, except for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Protein; Quality; Hydrolysis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000400015 |
| |
|
| |
| Registros recuperados: 181 | |
|
|
|
