|
|
|
| Registros recuperados: 40 | |
|
|
| Gueguen, Marielle; Bardouil, Michele; Baron, Regis; Lassus, Patrick; Truquet, Philippe; Massardier, Julie; Amzil, Zouher. |
| Contamination of shellfish by paralytic shellfish poisoning ( PSP) toxins poses an economic threat to shellfish farmers. As contaminated shellfish cannot be harvested for long periods of time, it would be very useful to develop processes to optimise and shorten their detoxification. In this study, Pacific oysters Crassostrea gigas were first experimentally contaminated over a period of 13 days with a continuous flow of toxic Alexandrium minutum cultures at concentrations ranging from 150 to 200 cell ml(-1) ( toxin content after 13 days of contamination 438 mu g STX equiv. 100 g(-1) wet weight). Then, two different detoxification treatments were tested and showed detoxification rates greater than those observed in coastal environments. The first treatment... |
| Tipo: Text |
Palavras-chave: Alexandrium minutum; Crassostrea gigas; Detoxification; Inorganic matter; Organic matter; PSP; Paralytic shellfish poisoning. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-3896.pdf |
| |
|
|
| Reveillon, Damien; Abadie, Eric; Sechet, Veronique; Brient, Luc; Savar, Veronique; Bardouil, Michele; Hess, Philipp; Amzil, Zouher. |
| beta-N-methylamino-L-alanine (BMAA) is a neurotoxic non-protein amino acid suggested to be involved in neurodegenerative diseases. It was reported to be produced by cyanobacteria, but also found in edible aquatic organisms, thus raising concern of a widespread human exposure. However, the chemical analysis of BMAA and its isomers are controversial, mainly due to the lack of selectivity of the analytical methods. Using factorial design, we have optimized the chromatographic separation of underivatized analogues by a hydrophilic interaction chromatography coupled to tandem mass spectrometry (HILIC-MS/MS) method. A combination of an effective solid phase extraction (SPE) clean-up, appropriate chromatographic resolution and the use of specific mass spectral... |
| Tipo: Text |
Palavras-chave: Cyanotoxins; BMAA; DAB; AEG; HILIC-MS/MS; Cyanobacteria; Bivalve mollusks; French Mediterranean. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00245/35579/34112.pdf |
| |
|
|
| Larrazabal, Me; Lassus, Patrick; Maggi, Pierre; Bardouil, Michele. |
| A preliminary study of modern marine dinoflagellate cysts has been performed in Vilaine Bay through winters 1986, 1987 and 1989. Direct observations of muddy sand samples and most probable number (MPN) method applied to cysts have evidenced a low win ter species diversity with Spiniferites spp. and Scrippsiella sp. cysts as predominant species. Change in relative importance of maximum species diversity area from ycar to year suggests a possible action of bottom currents. A study of dormant stages of toxie dinoflagellates, genus Alexandrium and Dinophysis, has corroborated the occurrence of A. minutum cysts (IO to 30 cysts.g-I sediment) at low amounts and absence of Dinophysis sp. resting stages. More generally, comparison of cysts in sediments with summer... |
| Tipo: Text |
|
| Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/00133/24472/22488.pdf |
| |
|
| |
|
|
| Medhioub, Amel; Medhioub, Walid; Amzil, Zouher; Sibat, Manoella; Bardouil, Michele; Ben Neila, Idriss; Mezghani, Salah; Hamza, Asma; Lassus, Patrick. |
| Karenia selliformis strain GM94GAB was isolated in 1994 from the north of Sfax, Gabes gulf, Tunisia. This species, which produces gymnodimine (GYM) a cyclic imine, has since been responsible for chronic contamination of Tunisian clams. A study was made by culturing the microalgae oil enriched Gaillard f/2 medium. The influence of growing conditions oil toxin content was studied, examining the effects of (i) different culture Volumes (0.25 to 40 litre flasks), (ii) two temperature ranges (17-15 degrees C et 20-21 degrees C) and (iii) two salinities (36 and 44). Chemical analyses were made by mass spectrometry coupled with liquid chromatography (LC-MS/MS). Results showed that (i) the highest growth rate (0.34 +/- 0.14 div d(-1)) was obtained at 20 degrees C... |
| Tipo: Text |
Palavras-chave: Mouse bioassay; Gymnodimine; Toxicity; Growth; Karenia selliformis. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-7383.pdf |
| |
|
| |
|
|
| Lassus, Patrick; Bardouil, Michele; Baron, Regis; Berard, Jean-baptiste; Masselin, Pierre; Truquet, Philippe; Pitrat, Jp. |
| Shellfish production, and production of marine bivalve molluscs in particular, is the main form of marine aquaculture in the European Union, with a production estimated at 1,200,000 tons in 1999 (Food and Agricultural Organization). Moreover, aquaculture production is rapidly expanding throughout Europe and is becoming a major economic resource in developing areas such as Northern Greece, Ireland, and northwestern Spain. These shellfish, mainly mussels, oysters, and clams, are produced in a natural environment. Due to variations in their environment, these otherwise edible species may however become unsafe for human consumption. Potential problems are amplified due to the nature of shellfish themselves: as they are filter feeders, they consume large... |
| Tipo: Text |
Palavras-chave: Detoxification; PSP toxin; Toxic algae; Bioaccumulation; Contaminant; Clam; Mussel; Oyster; Shellfish culture. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-2271.pdf |
| |
|
|
| Gueguen, Marielle; Lassus, Patrick; Laabir, Mohamed; Bardouil, Michele; Baron, Regis; Sechet, Veronique; Truquet, Philippe; Amzil, Zouher; Barille, Laurent. |
| The occurrence of new phytoplankton species in a coastal area may be explained by the import of shellfish containing whole live algal cells in their digestive tracts. Indeed, shellfish containing toxic algal cells can induce both primary contaminations in safe areas ( initially free from toxic microalgae), and secondary contaminations of other shellfish growing in the same area via the faeces of the imported animals. To mitigate this problem, shellfish need to be placed in a separate holding tank and their intestinal content purged. For a deeper understanding of the risks associated with transferring contaminated shellfish, oysters ( Crassostrea gigas) and mussels ( Mytilus edulis) were purposely fed either Alexandrium minutum or A. catenella (... |
| Tipo: Text |
Palavras-chave: Risk assessment; Toxic phytoplankton; Uptake; Gut passage; Mytilus edulis; Crassostrea gigas. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-3897.pdf |
| |
|
| |
|
|
| Medhioub, Walid; Gueguen, Marielle; Lassus, Patrick; Bardouil, Michele; Truquet, Philippe; Sibat, Manoella; Nejib, Medhioub; Philippe, Soudant; Mejdeddine, Kraiem; Amzil, Zouher. |
| In the Gulf of Gabes (Tunisia, Eastern Mediterranean sea), the grooved carpet shell Ruditapes decussatus has been seen to contain persistent levels of gymnodimine (GYM) for several years. The present experimental work represents the first attempt to assess detoxification kinetics of fast-acting toxins (FAT) in marine molluscs fed specific diets of non-toxic algae (Isochrysis galbana). To find an optimal detoxification method, two experiments were performed in which clams were first fed the toxic dinoflagellate Karenia selliformis to artificially contaminate them with GYM, thus simulating the effect of natural toxic episodes. As a second step, the same clams were fed a non-toxic algae, I. galbana. to speed up the detoxification process. Changes in toxin... |
| Tipo: Text |
Palavras-chave: Ruditapes decussates; Grooved carpet shell; Karenia selliformis; Gymnodimine; Toxicity; Detoxification. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/2010/publication-7488.pdf |
| |
|
|
| Mondeguer, Florence; Abadie, Eric; Herve, Fabienne; Bardouil, Michele; Sechet, Veronique; Raimbault, Virginie; Berteaux, Tom; Zendong, Suzie Zita; Palvadeau, Hubert; Amzil, Zouher; Hess, Philipp; Fessard, Valérie; Huguet, Antoine; Sosa, Silvio; Tubaro, Aurelia; Aráoz, Rómulo; Molgó, Jordi. |
| Pour tenter d’apporter des réponses aux questions soulevées par une précédente étude commanditée pour approfondir les connaissances sur Vulcanodinium rugosum, l’organisme producteur de la Pinnatoxine G (PnTX G) (Hess et al., 2012), les objectifs de cette nouvelle étude sont les suivants : - Elargissement de la zone initiale de prélèvement d’Ingril à toute la lagune méditerranéenne et mise à profit de l’augmentation du nombre d’échantillons pour vérifier l’impact de l’effet matrice dans l’analyse LC-SM/MS. - Mise en culture en masse de Vulcanodinium rugosum et - Conduite d’une expérience de contamination in vivo sur des moules pour vérifier l’accumulation de la PnTX-G et comprendre les mécanismes de la métabolisation des PnTXs et de ses composés associés..... |
| Tipo: Text |
|
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00285/39635/38126.pdf |
| |
|
|
| Lassus, Patrick; Amzil, Zouher; Bardouil, Michele; Baron, Regis; Bohec, Madeleine; Bougrier, Serge; Garen, Pierre; Laabir, Mohamed; Leguay, Didier; Masselin, Pierre; Truquet, Philippe; Sechet, Veronique; Bablon, Clotilde; Berard, Jean-baptiste; Carre, Camille; Cheignon, Valerie; Lecamp, Sylvain; Mabit, Marie. |
| L'objectif général de l'étude est de simuler les épisodes de contamination de certains bivalves (huîtres et moules) par les toxines d'Alexandrium minutum et A. calenella à partir d'un module expérimental adapté, et en vue de construire un modèle de contamination applicable à ces espèces. Ce modèle sera ensuite susceptible d'être couplé aux modèles biologiques locaux (estuaire de Penzé et étang de Thau) de déterminisme des blooms, ainsi qu'aux modèles hydrodynamiques développés par différentes équipes (Ifremer, Université de Montpellier). La première année (2001-2002) était consacrée à la simulation des contaminations en estuaire de Penzé (Alexandrium minulum) et au développement de cultures en masse d'A. calenella de Thau en bioréacteurs. |
| Tipo: Text |
|
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00156/26711/24786.pdf |
| |
|
|
| Lassus, Patrick; Bardouil, Michele. |
| A partir d'observations récentes en microscopie photonique et à balayage il est devenu nécessaire de réviser la dénomination de Dinophysis acuminata donnée à différentes espèces proches. Des échantillons phytoplanctoniques prélevés en différents points des côtes françaises ont été examinés par le Professeur BALECH en Argentine et les exemplaires de Méditerranée et des côtes sud bretonnes ont été identifiés comme D. sacculus. Compte tenu des données bibliographiques sur la répartition du genre Dinophysis sur les côtes françaises et du caractère toxique de certaines espèces, quelques recommandations sont émises concernant les dénombrements effectués dans le cadre du contrôle phytoplanctonique des eaux conchylicoles. |
| Tipo: Text |
|
| Ano: 1986 |
URL: http://archimer.ifremer.fr/doc/00119/23001/20831.pdf |
| |
|
|
| Lassus, Patrick; Bardouil, Michele; Baron, Regis; Garen, Pierre; Laabir, Mohamed; Leguay, Didier; Masselin, Pierre; Truquet, Philippe; Joret, Camille. |
| L'objectif de l'étude est de simuler les épisodes de contamination des huîtres par les toxines d'Alexandrium minufum à partir d'un module expérimental adapté, et en vue de construire un modèle de contamination applicable à cette espèce. Ce modèle sera ensuite susceptible d'être couplé au modèle biologique local (estuaire de Penzé) de déterminisme des blooms, ainsi qu'aux modèles hydrodynamiques développés par différentes équipes (Ifremer). |
| Tipo: Text |
|
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00156/26712/24787.pdf |
| |
|
| |
|
| |
|
| |
|
|
| Bardouil, Michele; Bohec, Madeleine; Bougrier, Serge; Lassus, Patrick; Truquet, Philippe. |
| An experimental flow-through system allowing determination of the feeding behaviour of individual molluscs was used to study oysters exposed to mixed diets composed of varying proportions of the diatom Thalassiosira weissflogii and two strains (toxic and nontoxic) of the dinoflagellate Alexandrium larnarense. Our results show that, when compared to a T. weissflogii unialgal diet, even a diatomltoxic dinoflagellate ratio as low in biomass as 90/10 reduced clearance rates and biodeposit production by oysters. Consumption was slightly but significantly decreased for a 50/50 diatomltoxic dinoflagellate mixture. For the toxic dinoflagellate unialgal diet, ingestion, absorption and consumption were completely inhibited. Thus, the inclusion of low amounts of... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Thalassiosira weissflogii; Alexandrium tamarense; Ecophysiologie; Toxine paralysante des coquillages; Crassostrea gigas; Thalassiosira weissflogii; Alexandrium tamarense; Ecophysiology; Paralytic shellfish poison. |
| Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/00017/12788/9729.pdf |
| |
|
|
| Lassus, Patrick; Bardouil, Michele; Ledoux, Martial; Murail, Ingrid; Bohec, Madeleine; Truquet, Philippe; Frémy, Jean-marc; Rohmer, Véronique. |
| It is difficult to apply sanitary standards for mollusc contamination by paralytic shellfish poisoning to animals not consumed whole. This problem is illustrated by the 1990 embargo on a Japanese shipment of frozen scallop muscle and gonad: the sanitary threshold applied to digestive gland (in Japan) does not guarantee that muscle and especially gonad are toxin-free. Accordingly, we performed experimental contaminations of scallops (Pecten maximus) from Port-en-Bessin (Normandy, France) using a toxic Japanese strain of Alexandrium tamarense. During the contamination/decontamination experiment on different tissues (digestive gland, muscle, gonad), extracts were obtained using cold acetic acid 0.1 N to maintain the perfect integrity of the toxin profile.... |
| Tipo: Text |
Palavras-chave: Alexandrium tamarense; Phycotoxines; Bioaccumulation; Profils toxiniques; Pecten maximus; Patinopecten yessoensis; Alexandrium tamarense; Phycotoxins; Bioaccumulation; Toxin profiles; Patinopecten yessoensis; Pecten maximus. |
| Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/00189/30075/28563.pdf |
| |
|
|
| Lassus, Patrick; Bardouil, Michele; Masselin, Pierre; Naviner, Magali; Truquet, Philippe. |
| A sea water recirculating flume was especially designed in the framework of this study. It improves physiological and ecophysiological evaluation of a 25 oysters (c. gigas) set fed on PSP toxic A. minutum cultures and then detoxified with commonly used aquaculture species (I. galbana, T. suecica, S. costatum, T. weissflogii). Chlorophyll a levels are kept steady in each case and adjusted in order to reach the same food value in each tank (0,5 mg/l TPM). When exposed to the mildly toxic species A. minutum (0,5 pg.eq.STX.cel-1) oysters depict a reduced clearance rate, food uptake and shell-valves activity (compared to non-toxic algal diets). The same physiological parameters do not show any significant differences when oysters are exposed to the control al... |
| Tipo: Text |
Palavras-chave: Épuration; Crassostrea gigas; Alexandrium minutum; PSP; Écophysiologie; Depuration; C. gigas; A. minutum; PSP; Ecophysiology. |
| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00139/25021/23126.pdf |
| |
| Registros recuperados: 40 | |
|
|
|
