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Registros recuperados: 61 | |
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Berthe, Franck; Burreson, Eugene; Hine, Mike. |
Recently, an increasing number of research teams are engaged in developing DNA-based diagnostic techniques for mollusc pathogens. These techniques are slowly moving from development in specialised laboratories for research purposes, to routine application and are expected to find an increasing use in routine disease monitoring programs in mollusc aquaculture and in efforts to prevent the spread of pathogens within and between nations. Considering the probable development and interest in these diagnostic techniques, the potential for wide applications in aquaculture and the inherent problems currently associated with their use, it appears necessary to address these issues. |
Tipo: Text |
Palavras-chave: Molecular tools; Mollusc disease; Diagnosis; Pathology. |
Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/publication-3291.pdf |
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Gay, Melanie; Berthe, Franck; Leroux, Frederique. |
In an attempt to develop a reproducible experimental model of bacterial infection in Crassostrea gigas, oysters taken from very localised sub-populations suffering natural mortality outbreaks were used in cohabitation trials under laboratory conditions. From these trials, a collection of Vibrio strains was isolated from moribund and healthy oysters. In a second step, strains were experimentally tested for virulence by means of injection into healthy oysters. This screening revealed a span of virulence among isolated strains from none to medium. When pooling injected strains, results suggest increased virulence. Vibrio strains may have additive/synergistic action leading to higher C, gigas mortality rates in experimental challenges. Although the study... |
Tipo: Text |
Palavras-chave: Crassostrea gias; Antagonism; Agonism; Virulence; Vibrio splendidus. |
Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-3174.pdf |
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Berthe, Franck. |
La pénéiculture calédonienne connait, depuis 1993, de sévères difficultés de développement et son expansion, spectaculaire au cours des six dernières années, est aujourd'hui considérablement ralentie. Le «syndrome 93», terme consacré par l' usage, affecte les élevages dans leur phase dite de grossissement, et se traduit par des taux de survie inférieurs aux seuils de rentabilité des fermes. Les pertes infligées à la filière mettent celle-ci en péril.C'est pourquoi un programme d'assistance par la recherche en pathologie des pénéides a été élaboré afin de répondre à ces difficultés. Le but de cette démarche est d'établir un plan de lutte basé sur la détection précoce de situations critiques, caractérisées par la présence d'un agent pathogène (diagnostic) et... |
Tipo: Text |
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Ano: 1995 |
URL: http://archimer.ifremer.fr/doc/00451/56240/57800.pdf |
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Gay, Melanie; Waechter, Magali; Lambert, Christophe; Escoubas, Jean-michel; Cochennec, Nathalie; Nicolas, Jean-louis; Berthe, Franck; Le Roux, Frédérique. |
Nowadays, cupped oyster, Crassostrea gigas, production is the main shellfish production in France. Spats cultivated on most farms are usually collected in the wild, but hatchery spats are becoming more numerous, and represent around 10% of today's cultivated spats. During the summer period, when the temperatures are higher, high mortality rates have been regularly observed in cupped in oyster C. gigas (60 to 100%) spats since 1991, both in hatcheries-nurseries and in their natural habitat. Over the last five years, these episodes have become a big concern for all the professionals. |
Tipo: Text |
Palavras-chave: Crassostrea gigas; Pathogène; Vibrio splendidus; Bactériologie. |
Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/acte-3277.pdf |
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Cochennec, Nathalie; Reece, K.; Berthe, Franck; Hine, Mike. |
Microcell-type parasites of oysters are associated with a complex of diseases in different oyster species around the world. The etiological agents are protists of very small size that are very difficult to characterize taxonomically. Associated lesions may vary according to the host species, and their occurrence may be related to variations in tissue structure. Lesion morphology cannot be used to distinguish the different agents involved. Ultrastructural observations on Mikrocytos roughleyi revealed similarities with Bonamia spp., particularly in regard to the presence of electron-dense haplosporosomes and mitochondria, whose absence from M mackini also indicate that M roughleyi and M mackini are not congeneric. A partial small subunit (ssu) rRNA gene... |
Tipo: Text |
Palavras-chave: Small subunit rDNA; Taxonomy; Microcell; Bonamosis; Saccostrea glomerata; Mikrocytos roughleyi. |
Ano: 2003 |
URL: https://archimer.ifremer.fr/doc/2003/publication-2816.pdf |
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Le Roux, Frédérique; Gay, Melanie; Lambert, Christian; Waechter, Magali; Poubalanne, Saravanne; Chollet, Bruno; Nicolas, Jean-louis; Berthe, Franck. |
French mollusc production is based mainly on the Pacific cupped oyster, Crassostrea gigas. Since 1991, annual mass mortality of juveniles has been reported during summer months. These recurring episodes concern professionals who fear that like Portugese oyster, C. angulata, C gigas could in turn disappear following one of these epizooties. Previously, bacteriological analysis of moribund oyster juveniles yielded an isolate of a Vibrio splendidus biovar H strain, named TNEMF6. This isolate was demonstrated to be pathogenic to Crassostrea gigas spat by experimental challenge. To study the association between summer oyster mortality and presence of TNEMF6 cluster strains, Vibrionaceae fauna were isolated from infected spat along the French Atlantic coast... |
Tipo: Text |
Palavras-chave: Vibrio splendidus; Virulence; Epidemiology; PCR RFLP; Oyster mortality. |
Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-565.pdf |
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Berthe, Franck; Prou, Jean. |
French Polyncsia is currently the world's largest producer of cultured black pearls with exports worth US$150 millions annually. The activity has become of prime socio-economic importance the archipelagos with strong support and control of the government. In the mid-1980s, mass mortality of the black-lipped pearl oyster, Pinctada margaritifera, has occurred in several atolls and challenged the development of pearl farming activity. Consequent Studies and surveys have described severa! pathologica! conditions. None of those is considered to pose significant threat to the industry. However, they are monitored by a surveillance program in order to prevent and control emerging diseases. The governmemt of French Polynesia has developed efforts to maintain high... |
Tipo: Text |
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Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/00353/46396/46032.pdf |
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Paillard, Christine; Nicolas, Jean-louis; Le Chevalier, Patrick; Lambert, Christophe; Le Boulay, Christine; Berthe, Franck; Haras, Dominique. |
The emergence of new vibriosis in the European Atlantic coasts has been associated with mass mortalities of molluscs causing important economic losses. For examples, Vibrio carchariae, a bacteria already isolated from shark, has been associated with a severe Haliotis epizooty in hatchery. In oysters, Crass()strea gigas, a strain closely related to Vibrio splendidus is suspected to be associated with summer mortalities. |
Tipo: Text |
Palavras-chave: Pathogenic vibrios; Bivales; Vibriosis; Bacteriology. |
Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/acte-3287.pdf |
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Goarant, Cyrille; Merien, Fabrice; Berthe, Franck; Mermoud, Isabelle; Perolat, Philippe. |
A molecular typing study on Vibrio strains implicated in shrimp disease outbreaks in New Caledonia and Japan was conducted by using AP-PCR (arbitrarily primed PCR). It allowed rapid identification of isolates at the genospecies level and studies of infraspecific population structures of epidemiological interest. Clusters identified within the species Vibrio penaeicida were related to their area of origin, allowing discrimination between Japanese and New Caledonian isolates, as well as between those from two different bays in New Caledonia separated by only 50 km. Other subclusters of New Caledonian V. penaeicida isolates could be identified, but it was not possible to link those differences to accurate epidemiological features. This contribution of AP-PCR... |
Tipo: Text |
Palavras-chave: Vibrio spp.; Shrimp; Molecular typing. |
Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/publication-2734.pdf |
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Renault, Tristan; Berthe, Franck; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe; Le Deuff, Rose-marie; Lipart, Cecile; Thebault, Anne. |
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la... |
Tipo: Text |
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae. |
Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/00043/15452/12827.pdf |
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Grizel, Henri; Berthe, Franck. |
Les 21 et 22 juin 2000, le Département des Ressources Aquacoles a consacré ses journées annuelles à une revue du thème "santé des cheptels" et du dispositif de recherche en pathologie sur les filières poissons, crustacés et mollusques, domaine très stratégique pour l'aquaculture marine française. Les objectifs des ces journées, rappelés par Yves Harache, Directeur du Département, étaient les suivante : • Dresser le bilan des actions de recherche menées par l'Ifremer en pathologie des espèces aquacoles, • Repréciser la stratégie (objectifs/moyens) du Département des Ressources Aquacoles sur le thème "santé des cheptels", • Programmer les actions de recherche à venir, • Stimuler les interactions entre groupes de recherche et laboratoires impliqués. Le... |
Tipo: Text |
Palavras-chave: Pathologie; Ecotoxicologie; Mortalités; Ecopathologie; Poissons; Crustacés; Mollusques; Nacres. |
Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00164/27547/25711.pdf |
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Bondad-reantaso, Melba; Berthe, Franck. |
Pacific oysters were introduced into west coast of the USA from Matsushima Bay in Japan, infected with low levels of Haplosporidiumsp. identical to H.nelsoni(MSX) which causes high mortalities of Eastern oyster (C. virginica). A highly specific and sensitive DNA probe for H. nelsonican also detect Haplosporidium sp. in C.gigas of western US and Japan (Burreson and Stokes 2000).In 2002, H. nelsoni was detected in Japan using the same probe (Kamaishi and Yoshinaga 2002).Lesson: it appears that H. nelsoni does not cause serious disease in Pacific oysters; H. nelsoniis now speculated to have been introduced into the Pacific US by apparently healthy but infected C. gigas. Infected C. gigaswere introduced onto the east coast of the US where the parasite shifted... |
Tipo: Text |
Palavras-chave: Pathogens; Diseases; Impact; Introductions; Transfers; Molluscan Pathogens. |
Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/acte-3294.pdf |
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Le Roux, Frédérique; Audemard, Corinne; Cochennec, Nathalie; Thebault, Anne; Berthe, Franck. |
Responsible for massive epizootics, the Marteilia type parasites are very dangerous infectious agents for oysters. As a matter of fact, in France the spreading out of the parasitosis caused by Marteilia refringens disturbed dangerously the flat oyster, Ostrea edulis, production; destroying a large percentage of the farms on the Atlantic coast. The fight against marteiliose implies a good management of the contamination risk, that is to say the identification of the parasite(s), the characterization of the host(s), vector(s) or reservoir(s) and the understanding of the parasite's transmission mode. |
Tipo: Text |
Palavras-chave: Bivalves; Biologie Moléculaire; Marteilia refringens; Epidémiologie. |
Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/acte-3271.pdf |
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Ngo, Thao; Berthe, Franck; Choi, Kwang-sik. |
The occurrence of Marteilioides chungmuensis, a protozoan paramyxean parasite in the reproductive system of the Pacific oyster Crassostrea gigas, was observed at Gosung Bay, Korea. Seasonal variation in gonad development was investigated in a suspended cultured oyster population. Gametogenesis began in February and first-spawning was observed between mid and late June when surface water temperature reached 22 to 25degreesC. Spawning activity extended from mid June to late September, with 2 marked spawning peaks in June and August. Histological examination indicated that gonad development paralleled seasonal fluctuations in water temperature. Spawning in late June was partly associated with a sudden drop in salinity due to large freshwater inputs to the Bay... |
Tipo: Text |
Palavras-chave: Korea; Ovary parasite; Marteilioides chungmuensis; Gonad development; Crassostrea gigas. |
Ano: 2003 |
URL: https://archimer.ifremer.fr/doc/2003/publication-2848.pdf |
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Renault, Tristan; Berthe, Franck; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe; Le Deuff, Rose-marie; Thuillier, Benoit. |
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la... |
Tipo: Text |
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae. |
Ano: 1995 |
URL: http://archimer.ifremer.fr/doc/00043/15453/12828.pdf |
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Escoubas, Jean-michel; Montagnani, Caroline; Le Roux, Frédérique; De Lorgeril, Julien; Berthe, Franck; Bachere, Evelyne. |
We isolated complementary DNA (Cg-TIMP) from Crassostrea gigas that codes for a protein highly homologous to vertebrate tissue inhibitors of metalloproteinases (TIMPs). In vertebrates, TIMPs are multifunctional proteins that play a key role in extracellular matrix metabolism. They are involved in physiological processes (e.g. embryonic development, damage repair, angiogenesis) and pathological processes (e.g. inflammatory reactions, cancer). |
Tipo: Text |
Palavras-chave: Proteine; Cg TIMP; ADN; Crassostrea gigas. |
Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/acte-3268.pdf |
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Cochennec, Nathalie; Le Roux, Frédérique; Berthe, Franck; Thebault, Anne. |
Bonamiose, a disease caused by the protozoa Bonamia ostreae, has destroyed the flat oyster Ostrea edulis production, in France but also in Europe and in the United States since 1979. Another species of Bonamia has been monitored in New-Zealand, in Australia and lately in Chile. In Canada and Australia, two other parasites have been described in cupped oyster: Mikrocytos mackini and Mikrocytos roughleyi. All these small size protozoa are grouped under the name "Mikrocell". For most of them, light and electronic microscope descriptions have been realised. Based on those criteria, different taxonomic positions have been proposed. However, these analysis are still questioned today. |
Tipo: Text |
Palavras-chave: Bivalves; Haplosporidia; Parasites; Pathologie. |
Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/acte-3270.pdf |
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Registros recuperados: 61 | |
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