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| Registros recuperados: 18 | |
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| Comps, Michel; Mari, Jocelyne; Poisson, Francois; Bonami, Jean-robert. |
| A cytoplasmic dsRNA virus, rotifer birnavirus (RBV), has recently been isolated from the rotifer Brachionus plicatilis and is associated with a high mortality rate. Histologically, the viral lesions consist of characteristic inclusions, particularly amorphous dense bodies containing occluded particles. Purified virions are about 59 nm in diameter, single-shelled and display four capsomers per edge. The purified virions have a buoyant density of 1.290 (full particles) and 1.250 (empty particles) in CsCI gradients. Four major structural polypeptides of Mrs 60K, 52K, 33K and 27K were detected by SDS-PAGE. The genome is composed of two linear segments of dsRNA with Mrs of 2.45 x 106 and 2.31 × 106; additionally, small circular ssRNA molecules were detected by... |
| Tipo: Text |
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| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/00129/24032/21994.pdf |
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| Mari, Jocelyne; Poulos, Bonnie; Lightner, Donald; Bonami, Jean-robert. |
| The single-stranded genomic RNA of Taura syndrome virus (TSV) is 10205 nucleotides in length, excluding the 3' poly(A) tail, and contains two large open reading frames (ORFs) that are separated by an intergenic region of 207 nucleotides. The ORFs are flanked by a 377 nucleotide 5' untranslated region (UTR) and a 226 nucleotide 3' UTR followed by a poly(A) tail. The predicted amino acid sequence of ORF1 revealed sequence motifs characteristic of a helicase, a protease and an RNA-dependent RNA polymerase, similar to the non-structural proteins of several plant and animal RNA viruses. In addition, a short amino acid sequence located in the N-terminal region of ORF1 presented a significant similarity with a baculovirus IAP repeat (BIR) domain of inhibitor of... |
| Tipo: Text |
Palavras-chave: Genomic characterization; Taura syndrome; Virus; Shrimp disease; Penaeidae. |
| Ano: 2002 |
URL: https://archimer.ifremer.fr/doc/2002/publication-715.pdf |
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| Renault, Tristan; Haffner, Philippe; Baudin Laurencien, F.; Breuil, Gilles; Bonami, Jean-robert. |
| Since 1986, an unknown disease causing high mortalities appeared in Ifremer's Pacific OceanologicaI Center (Tahiti) nursery of sea bass (Lates calcarifer). opathology of lesions involving both centraI nervous system (CNS) and eye retina, and the tuation exists in South-East Asian countries (Indonesia, Philippines, Malaysia and Singapore), and in northem Australia. This paper describes the histmorphology of a new fish virus, which is believed to be the aetiological agent of high mortalities among sea bass larvae. |
| Tipo: Text |
Palavras-chave: Virus like; Lates calacrifer; Hatchery; Mortality. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/publication-2885.pdf |
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| Cousserans, François; Bonami, Jean-robert; Comps, Michel; Grizel, Henri. |
| Cell cultures, which are the basis of numerous recent studies in medical pathology, have been, for a few years, of great interest in the studies carried out on the diseases affecting invertebrates. However, such cultures, dealing with marine bivalve molluscs, obtained for the first time by Vago and Chastang (1) and progressively improved since then [(2) to (10)], have not been used in pathological studies yet. As the diseases affecting marine molluscs of economic importance such as oysters are both of great interest and facing specific study difficulties, we tried to adapt oyster cell cultures to the study of the presence, development and cycles of the pathogenic or possibly pathogenic microorganisms affecting Crassostrea, and especially the species gigas,... |
| Tipo: Text |
Palavras-chave: Culture cellule; Parasite; Huîtres; Crassostrea gigas. |
| Ano: 1974 |
URL: http://archimer.ifremer.fr/doc/1974/publication-5907.PDF |
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| Poulos, Bonnie; Tang, K; Pantoja, C; Bonami, Jean-robert; Lightner, D. |
| The causative agent of myonecrosis affecting cultured Penaeus vannamei in Brazil was demonstrated to be a virus after purification of the agent from infected shrimp tissues. Purified viral particles were injected into specific pathogen-free P. vannamei, resulting in a disease that displayed the same characteristics as those found in the original shrimp used for purification. The virus was named infectious myonecrosis virus (IMNV). The viral particles were icosahedral in shape and 40 nm in diameter, with a buoyant density of 1(.)366 g ml(-1) in caesium chloride. The genome consisted of a single, double-stranded (dsRNA) molecule of 7560 bp. Sequencing of the viral genome revealed two non-overlapping open reading frames (ORFs). The 5' ORF (ORF 1, nt 136-4953)... |
| Tipo: Text |
Palavras-chave: Purification; Myonecrosis; Virus; Shrimp; Penaeid. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1504.pdf |
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| Bonami, Jean-robert. |
| The trembling of legs followed by increasing paralysis and eventual death, were recorded on specimens of the above sp from Sete region. EM studies of tissue showed paraspherical bodies measuring approx 61 nm with a size range of 58-65 nm. By experimentally infecting specimens with a purified viral suspension the following pathogenesis was observed: in greater than 60 per cent of the specimens, trembling of the legs began within 6 days, growing very pronounced on the 8th day, leading to a general paralysis and 9 days after the infection a mortality rate of 70-80 per cent was recorded. The following characteristics of the virus are noted: its cytoplasmic localisation, paraspherical form, size ranging from 60-70 nm and the absence of an envelope. Considering... |
| Tipo: Text |
Palavras-chave: Paralysis; Virus; Decapoda. |
| Ano: 1973 |
URL: http://archimer.ifremer.fr/doc/1973/publication-2151.pdf |
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| Grizel, Henri; Comps, Michel; Bonami, Jean-robert; Cousserans, F.; Duthoit, Jean-louis; Le Pennec, Marie-annick. |
| Numerous diseases have been described in Ostrea and Crassostrea oysters and especially in cultivated species of commercial interest. The lack of growth, but more often mortalities, are in most cases the most obvious symptoms of this supposed sickness. The consequences of these epizooties can be so bad that they can result in the disappearance of an entire oyster population from a determined area as for Ostrea edulis L. in 1920-1921 (Dollfus, 1922) on some coasts of Europe and for Crassostrea angulata LMK in 1970-1971 on the French Atlantic coast and especially in the Marennes-Oléron and Arcachon basins. These cases can be considered as extreme cases as most of the time, oysters are only subjected to a number of episodic affections, affecting the farms only... |
| Tipo: Text |
Palavras-chave: Glande digestive; Maladie; Pathologie; Ostrea edulis; Huître. |
| Ano: 1974 |
URL: http://archimer.ifremer.fr/doc/1974/publication-6286.PDF |
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| Mari, Jocelyne; Bonami, Jean-robert; Lightner, Donald V. |
| The ssRNA genome extracted from purified Taura Syndrome Virus (TSV) was transcribed into double-stranded, blunt-ended cDNA and was used to construct cDNA libraries either in pUC 18 or in pBluescript II KS-vectors. Twelve recombinant plasmids chosen after screening of the libraries were subjected to restriction enzyme digestions for determination of size inserts and restriction maps. Two of them, pP15 and pQ1, were selected for probe construction. The inserts, 1500 and 1300 base pairs (bp) respectively, were DIG-11dUTP-labelled and the corresponding probes were named P15 and Q1. On northern blots and dot blots, using different denaturation methods, the 2 probes hybridized specifically with extracted RNA-TSV genome, TSV and infected TS shrimp homogenates. No... |
| Tipo: Text |
Palavras-chave: TSV; Penaeid shrimp; Picornavirus; Cloning; Dot blot hybridization; In situ hybridization. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00447/55899/57914.pdf |
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| Bazin, F.; Monsarrat, P.; Bonami, Jean-robert; Croizier, G.; Meynadier, G.; Quiot, J.m.; Vago, C.. |
| A new virus from the Crab Carcinus maenas has been found in the nuclei of conjunctive and blood cells. Its localization, morphology, size, morphogeny relate it to the Baculovirus group. By some aspects it appears to be related closer to the insect viruses from Oryctes rhinoceros, and to red mite viruses from Panonychus ulmi and P.citri than to the nuclear polyhedrosis of the pink shrimp Penaeus duorarum. |
| Tipo: Text |
Palavras-chave: Penaeus duorarum; Panonychus citri; Panonychus ulmi; Oryctes rhinoceros; Carcinus maenas; Viruses; Viral diseases. |
| Ano: 1974 |
URL: http://archimer.ifremer.fr/doc/1974/publication-2041.pdf |
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| Comps, Michel; Bonami, Jean-robert; Vago, C.; Razet, Daniel. |
| Among the major epizootics known in marine Molluscs, the one that started on the coasts of Europe in 1970 in the Portuguese oyster, Crassostrea angulata, was certainly one of the largest due to its range and due to the economic loss sustained by the French oyster farming as a whole. It led to the disappearance of the species C. angulata as a farmed oyster, and to the massive introduction of the Japanese oyster, C. gigas, known to be resistant to the disease. From the first manifestations of this epizootic, the laboratories of the ISTPM were actively dedicated to its etiology. Now, after several years, a study begun at the La Tremblade laboratory has just been completed. |
| Tipo: Text |
Palavras-chave: Marennes Oléron; Mortalités; Histologie; Epizootie; Crassostrea angulata. |
| Ano: 1976 |
URL: http://archimer.ifremer.fr/doc/1976/publication-2995.pdf |
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| Bonami, Jean-robert; Grizel, Henri; Vago, Constantin; Dutholt, Jean-louis. |
| Digestive glands of Ostrea edulis affected by an epizootic have been examined by light- and electron- microscopy. Round, compact lesions in the digestive gland cells contain refractive elements staining slightly blue or red with Geimsa. Ultra-structurally, each lesion contains (a) dense cytoplasma, without mitochondria and with paracrystal-line inclusions, (b) enclosed regions with vacuolar cytoplasm, no mitochondria and two types of formation; one is opaque, the other a vacuole containing ribosomes, lamellar structures and peripheral ovoid particles. The nature and significance of these structures is not known. |
| Tipo: Text |
Palavras-chave: Histology; Epizooty; Ostrea edulis. |
| Ano: 1971 |
URL: http://archimer.ifremer.fr/doc/1971/publication-2238.pdf |
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| Registros recuperados: 18 | |
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