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	Provedor de dados 56 (6) Autor Borojevic,R. (6) El-Cheikh,M.C. (2) Aguiar,A.M. (1) Amaral,L.M. (1) Baptista,L.S. (1) Barros,A.P.D.N. (1) Costa,E.S. (1) Costa,M.L. (1) Dutra,F.F. (1) Dutra,H.S. (1) Freitas,M.S. (1) Garzoni,L.R. (1) Gomes,L. (1) Guerra,M.C. (1) Maia,S.F. (1) Meirelles,M.N. (1) Mermelstein,C.S. (1) Oliveira,F.L. (1) Oliveira,G.A.P. (1) Otazu,I.B. (1) Mais... Palavra-chave B lymphocytes (1) Bone marrow (1) Cell differentiation cell proliferation (1) Cell migration (1) Children (1) Chronic myeloid leukemia (1) EGTA Myoblasts Desmin Intermediate filaments RT-PCR (1) Ectoenzyme (1) Exopeptidase (1) Extracellular Ca2+ (1) Extracellular matrix glycoconjugate adhesion (1) Hematopoiesis (1) Imatinib mesylate (1) Immunoglobulin switch (1) Membrane rafts (1) Minimal residual disease (1) Multicellular spheroid (1) Schistosomiasis (1) Stroma (1) Three-dimensional culture (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Info:eu-repo/semantics/other (1) Info:eu-repo/semantics/report (1) Ano 2010 (1) 2005 (3) 2003 (1) 1999 (1) País Brazil (6) Idioma Inglês (6)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 6 Primeira ... 1 ... Última Multicellular spheroids of bone marrow stromal cells: a three-dimensional in vitro culture system for the study of hematopoietic cell migration 56 Rossi,M.I.D.; Barros,A.P.D.N.; Baptista,L.S.; Garzoni,L.R.; Meirelles,M.N.; Takiya,C.M.; Pascarelli,B.M.O.; Dutra,H.S.; Borojevic,R.. Cell fate decisions are governed by a complex interplay between cell-autonomous signals and stimuli from the surrounding tissue. In vivo cells are connected to their neighbors and to the extracellular matrix forming a complex three-dimensional (3-D) microenvironment that is not reproduced in conventional in vitro systems. A large body of evidence indicates that mechanical tension applied to the cytoskeleton controls cell proliferation, differentiation and migration, suggesting that 3-D in vitro culture systems that mimic the in vivo situation would reveal biological subtleties. In hematopoietic tissues, the microenvironment plays a crucial role in stem and progenitor cell survival, differentiation, proliferation, and migration. In adults, hematopoiesis... Tipo: Info:eu-repo/semantics/article Palavras-chave: Multicellular spheroid; Three-dimensional culture; Cell migration; Bone marrow. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000002 Positive response to imatinib mesylate therapy for childhood chronic myeloid leukemia 56 Oliveira,G.A.P.; Costa,E.S.; Freitas,M.S.; Dutra,F.F.; Maia,S.F.; Guerra,M.C.; Tabernero,M.D.; Borojevic,R.; Otazu,I.B.; Silva,J.L.. Chronic myeloid leukemia (CML) is rare in the pediatric population, accounting for 2-3% of childhood leukemia cases, with an annual incidence of one case per million children. The low toxicity profile of imatinib mesylate has led to its approval as a front-line therapy in children for whom interferon treatment has failed or who have relapsed after allogeneic transplantation. We describe the positive responses of 2 children (case 1 - from a 7-year-old male since May 2005; case 2 - from a 5-year-old female since June 2006) with Philadelphia-positive chromosome CML treated with imatinib (300 mg/day, orally) for up to 28 months, as evaluated by morphological, cytogenetic, and molecular approaches. Our patients are alive, are in the chronic phase, and are in... Tipo: Info:eu-repo/semantics/report Palavras-chave: Chronic myeloid leukemia; Imatinib mesylate; Minimal residual disease; Children. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600009 Extracellular matrix: understanding the complexity 56 Borojevic,R.. Tipo: Info:eu-repo/semantics/other Palavras-chave: Extracellular matrix glycoconjugate adhesion; Cell differentiation cell proliferation. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500001 IgE expression on the surface of B1 and B2 lymphocytes in experimental murine schistosomiasis 56 Oliveira,F.L.; Aguiar,A.M.; Borojevic,R.; El-Cheikh,M.C.. In a previous study we monitored the distribution and phenotype expression of B1 cells during the evolution of experimental murine schistosomiasis mansoni and we proposed that the B1 cells were heterogeneous: a fraction which originated in the spleen and followed the migratory pathway to mesenteric ganglia, while the other was the resident peritoneal B1-cell pool. In the present study, we have addressed the question of whether these two B1-lymphocyte populations are involved in the production of the late Ig isotype IgE, which is present in high levels in schistosomal infection. Lymphocyte expression of surface markers and immunoglobulins were monitored by immunofluorescence flow cytometry. Both in the spleen and mesenteric ganglia, the B1 and B2 cells were... Tipo: Info:eu-repo/semantics/article Palavras-chave: Schistosomiasis; B lymphocytes; Immunoglobulin switch. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700006 Dipeptidyl peptidase IV (CD26) activity in the hematopoietic system: differences between the membrane-anchored and the released enzyme activity 56 Pereira,D.A.; Gomes,L.; El-Cheikh,M.C.; Borojevic,R.. Dipeptidyl peptidase IV (DPP-IV; CD26) (EC 3.4.14.5) is a membrane-anchored ectoenzyme with N-terminal exopeptidase activity that preferentially cleaves X-Pro-dipeptides. It can also be spontaneously released to act in the extracellular environment or associated with the extracellular matrix. Many hematopoietic cytokines and chemokines contain DPP-IV-susceptible N-terminal sequences. We monitored DPP-IV expression and activity in murine bone marrow and liver stroma cells which sustain hematopoiesis, myeloid precursors, skin fibroblasts, and myoblasts. RT-PCR analysis showed that all these cells produced mRNA for DPP-IV. Partially purified protein reacted with a commercial antibody to CD26. The K M values for Gly-Pro-p-nitroanilide ranged from 0.43 to 0.98... Tipo: Info:eu-repo/semantics/article Palavras-chave: Hematopoiesis; Stroma; Exopeptidase; Ectoenzyme; Membrane rafts. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500003 Changes in cell shape and desmin intermediate filament distribution are associated with down-regulation of desmin expression in C2C12 myoblasts grown in the absence of extracellular Ca2+ 56 Mermelstein,C.S.; Amaral,L.M.; Rebello,M.I.L.; Reis,J.S.N.; Borojevic,R.; Costa,M.L.. Desmin is the main intermediate filament (IF) protein of muscle cells. In skeletal muscle, desmin IFs form a scaffold that interconnects the entire contractile apparatus with the subsarcolemmal cytoskeleton and cytoplasmic organelles. The interaction between desmin and the sarcolemma is mediated by a number of membrane proteins, many of which are Ca2+-sensitive. In the present study, we analyzed the effects of the Ca2+ chelator EGTA (1.75 mM) on the expression and distribution of desmin in C2C12 myoblasts grown in culture. We used indirect immunofluorescence microscopy and reverse transcription polymerase chain reaction (RT-PCR) to analyze desmin distribution and expression in C2C12 cells grown in the presence or absence of EGTA. Control C2C12 myoblasts... Tipo: Info:eu-repo/semantics/article Palavras-chave: Extracellular Ca2+; EGTA Myoblasts Desmin Intermediate filaments RT-PCR. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700005 Registros recuperados: 6 Primeira ... 1 ... Última

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