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| Registros recuperados: 41 | |
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| Perrichon, Prescilla; Le Bihanic, Florane; Bustamante, Paco; Le Menach, Karyn; Budzinski, Helene; Cachot, Jerome; Cousin, Xavier. |
| Due to hydrophobic and persistent properties, polycyclic aromatic hydrocarbons (PAHs) have a high capacity to accumulate in sediment. Sediment quality criteria, for the assessment of habitat quality and risk for aquatic life, include understanding the fate and effects of PAHs. In the context of European regulation (REACH and Water Framework Directive), the first objective was to assess the influence of sediment composition on the toxicity of two model PAHs, benzo[a]pyrene and fluoranthene using 10-day zebrafish embryo-larval assay. This procedure was undertaken with an artificial sediment in order to limit natural sediment variability. A suitable sediment composition might be then validated for zebrafish and proposed in a new OECD guideline for chemicals... |
| Tipo: Text |
Palavras-chave: Fish embryo-larval assay; PAHs; Artificial sediment; EROD; Behavioral performance; Teratogenicity. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00242/35323/37905.pdf |
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| Coquille, Nathalie; Maurer, Daniele; Auby, Isabelle; Budzinski, Helene; Stachowski-haberkorn, Sabine; Morin, Soizick; Parlanti, Edith; Vedrenne, J.; Jan, G.; Menard, Dominique; Dupraz, Valentin; Rigouin, Loic; Gouriou, Laure; D'Amico, Florence; Tournaire, Marie-pierre; Meteigner, Claire; Perriere-rumebe, Myriam; Mounguengui, A.; Tapie, Nathalie; Chevance-demars, L.; Pardon, P.; Geneste, E.. |
| Tipo: Text |
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| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00276/38740/37283.pdf |
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| Le Bihanic, Florane; Morin, Benedicte; Cousin, Xavier; Le Menach, Karyn; Budzinski, Helene; Cachot, Jerome. |
| A new gravel-contact assay using rainbow trout, Oncorhynchus mykiss, embryos was developed to assess the toxicity of polycyclic aromatic hydrocarbons (PAHs) and other hydrophobic compounds. Environmentally realistic exposure conditions were mimicked with a direct exposure of eyed rainbow trout embryos incubated onto chemical-spiked gravels until hatching at 10 °C. Several endpoints were recorded including survival, hatching delay, hatching success, biometry, developmental abnormalities, and DNA damage (comet and micronucleus assays). This bioassay was firstly tested with two model PAHs, fluoranthene and benzo[a]pyrene. Then, the method was applied to compare the toxicity of three PAH complex mixtures characterized by different PAH compositions: a pyrolytic... |
| Tipo: Text |
Palavras-chave: PAH mixture; Oil extract; Pyrolytic extract; Embryotoxicity; Developmental defects; Genotoxicity. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00188/29919/28384.pdf |
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| Gamain, Perrine; Feurtet-mazel, Agnes; Maury-brachet, Regine; Auby, Isabelle; Pierron, Fabien; Belles, Angel; Budzinski, Helene; Daffe, Guillemine; Gonzalez, Patrice. |
| Dwarf eelgrasses (Zostera noltei) populations have decreased since 2005 in Arcachon Bay (southwest France). Various stressors have been pointed out, however the role of xenobiotics like pesticides or copper (Cu) and of parameters like water temperature warming have not yet been explored. To determine their impact, Z. noltei individuals were collected in a pollution-free site and transferred to the laboratory in seawater microcosms. This dwarf eelgrass was exposed to a pesticide cocktail and copper, alone or simultaneously, at temperatures (10 °C, 20 °C, 28 °C) representative of different seasons. After a two-week contamination, leaf growth, leaf bioaccumulation of Cu, and differential expression of target genes were studied. Eelgrasses bioaccumulated Cu... |
| Tipo: Text |
Palavras-chave: Zostera noltei; Arcachon Bay; Pesticides; Copper; Cellular impacts. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00407/51831/58536.pdf |
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| Vignet, Caroline; Le Menach, Karyn; Mazurais, David; Lucas, Julie; Perrichon, Prescilla; Le Bihanic, Florane; Devier, Marie-hélène; Lyphout, Laura; Frere, Laura; Begout, Marie-laure; Zambonino-infante, Jose Luis; Budzinski, Helene; Cousin, Xavier. |
| The release of polycyclic aromatic hydrocarbons (PAHs) into the environment has increased very substantially over the last decades leading to high concentrations in sediments of contaminated areas. To evaluate the consequences of long-term chronic exposure to PAHs, zebrafish were exposed, from their first meal at 5 days post fertilisation until they became reproducing adults, to diets spiked with three PAH fractions at three environmentally relevant concentrations with the medium concentration being in the range of 4.6–6.7 μg g−1 for total quantified PAHs including the 16 US-EPA indicator PAHs and alkylated derivatives. The fractions used were representative of PAHs of pyrolytic (PY) origin or of two different oils of differing compositions, a heavy fuel... |
| Tipo: Text |
Palavras-chave: Danio rerio; Life cycle; Polycyclic aromatic hydrocarbon; Heavy oil; Light crude oil; Alkylated PAH; Jaw; Digestion. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00188/29907/28490.pdf |
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| Barranger, Audrey; Akcha, Farida; Rouxel, Julien; Brizard, Raphael; Maurouard, Elise; Pallud, Marie; Menard, Dominique; Tapie, N.; Budzinski, Helene; Burgeot, Thierry; Benabdelmouna, Abdellah. |
| Pesticides represent a major proportion of the chemical pollutants detected in French coastal waters and hence a significant environmental risk with regards to marine organisms. Commercially-raised bivalves are particularly exposed to pollutants, among them pesticides, as shellfish farming zones are subject to considerable pressure from agricultural activities on the mainland. The aims of this study were to determine (1) the genotoxic effects of diuron exposure on oyster genitors and (2) the possible transmission of damaged DNA to offspring and its repercussions on oyster fitness. To investigate these points, oysters were exposed to concentrations of diuron close to those detected in the Marennes-Oleron Basin (two 7-day exposure pulses at 0.4 and 0.6 μg... |
| Tipo: Text |
Palavras-chave: Herbicide; Diuron; Oyster; Genotoxicity; Comet assay; Cytogenetics. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00162/27311/25541.pdf |
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| Tapie, Nathalie; Munaron, Dominique; Gonzalez, Jean-louis; Budzinski, Helene. |
| With the new European legislation (the Water Framework Directive, 2000/60/EC), the decision makers responsible for the assessment of the environmental contamination require simple monitoring tools. Passive sampling methods seem to be promising tools for the sampling of polar organic compounds in water, especially in marine waters where important dilutions and low concentrations are usual. They are taking into account the temporal variability and integrating the contamination over period of time. This work presents a study, on a large scale, of the contamination of French Mediterranean coastal waters realized in partnership with the Water Agency Rhône Méditerranée Corse (AERMC), and Coastal Water Quality Offices (CQEL). |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00031/14204/11473.pdf |
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| Vignet, Caroline; Devier, Marie-hélène; Le Menach, Karyn; Lyphout, Laura; Potier, Jeremy; Cachot, Jerome; Budzinski, Helene; Begout, Marie-laure; Cousin, Xavier. |
| A natural sediment spiked with three individual polycyclic aromatic hydrocarbons (PAHs; pyrene, phenanthrene and benzo[a]pyrene) was used to expose zebrafish embryos and larvae during 4 days. The total PAH concentration was 4.4 μg g−1 which is in the range found in sediment from contaminated areas. Quantification of metabolites in the larvae after exposure confirmed the actual contamination of the larvae and indicated an active metabolism especially for pyrene and benzo[a]pyrene. After a transfer in a clean medium, the larvae were reared to adulthood and evaluated for survival, growth, reproduction, and behavior. Measured endpoints revealed a late disruption of growth (appearing at 5 months) and a trend toward a lower reproductive ability. Adults of... |
| Tipo: Text |
Palavras-chave: Danio rerio; Polycyclic aromatic hydrocarbon; Lifecycle; Anxiety-like behavior; Offspring. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00188/29909/28497.pdf |
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| Auby, Isabelle; Bost, Charles-andré; Budzinski, Helene; Dalloyau, Sebastien; Desternes, Amandine; Belles, Angel; Trut, Gilles; Plus, Martin; Pere, Caroline; Couzi, Laurent; Feigne, Claude; Steinmetz, Julien. |
| While they had previously presented a hight stability, unlike in many coastal sites worldwide, eelgrass beds (Zostera noltii and Zostera marina) of Arcachon Bay have declined sharply since the 2000s. Various causes have been invoked to explain this phenomenon: climate anomalies, disease, over-grazing by herbivorous birds, contamination by herbicides, mechanical destruction during clam fishing. A multidisciplinary study was set up to study the possible effect of these factors, by putting into perspective the history of the seagrass beds and the temporal evolution of these parameters. After a chapter dealing with recent trends (2007-2010) of seagrasses in the Bay of Arcachon, the results relative to the various hypotheses are presented. These results allow... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00054/16507/14018.pdf |
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| Turquet, Jean; Nicet, Jean Benoit; Cambert, Harold; Denis, Yves; Gonzalez, Jean-louis; Bigot, Lionel; Guyomarch, Julien; Budzinski, Helene; Tapie, Nathalie; Jamon, Alban; Pribat, B. |
| Le tome 2 de l‘étude de « définition des réseaux de surveillance de la qualité des masses d’eau côtières de l’île de Mayotte », présente sous forme cartographique les données bancarisées sous format MapInfo. Seules les études intéressantes vis à vis de la DCE et disponibles ont été référencées et prises en compte dans cet exercice (cf. références bibliographique du tome 2). Ce premier exercice de bancarisation, ne se veut pas exhaustif, et devra faire l’objet d’entretiens, d’ajouts réguliers. |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00035/14622/11901.pdf |
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| Gonzalez, Jean-louis; Turquet, Jean; Cambert, Harold; Budzinski, Helene; Tapie, Nathalie; Guyomarch, Julien; Andral, Bruno. |
| Ce projet a permis de mettre en évidence le potentiel des techniques d'échantillonnage passif (DGT, POCIS, SBSE) à "grande échelle" (18 masses d'eau côtières: 6 récifales, 10 "mer ouverte" et 2 portuaires) au cours de 2 campagnes réalisées en période sèche et humide (post cyclonique). L'étude montre l'opérationnalité de ces systèmes en terme de réduction des coûts et de rapidité d'obtention des résultats, mais aussi en terme de facilité de mise en œuvre (par du personnel préalablement formé). Ces techniques ont pu être transférées aux acteurs locaux en charge de la surveillance de l'état de la contamination chimique des eaux. Les résultats ont contribué à la caractérisation des masses d'eau par rapport à certains contaminants chimiques (métaux, composés... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00020/13170/10228.pdf |
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| Perrichon, Prescilla; Akcha, Farida; Le Menach, Karyn; Goubeau, Manon; Budzinski, Helene; Cousin, Xavier; Bustamante, Paco. |
| In recent decades, PAH emissions due to extensive anthropogenic activities have risen sharply causing considerable pollution of aquatic ecosystems. This pollution represents a threat for organisms, among them are fish. Consequently, prenatal stress can have important repercussions, and may impact survival and population recruitment. To investigate this point, eggs were collected from zebrafish exposed during 6 months by trophic route to three aromatic fractions from two different origins, pyrolytic (PY) and petrogenic (light (BAL) and heavy (HFO) fractions) sources. Chronic dietary exposure of the parents was performed at environmentally relevant concentrations (0.3 ×, 1 × and 3 ×; 1 × represents an environmental concentration measured in French estuary).... |
| Tipo: Text |
Palavras-chave: Polycyclic aromatic hydrocarbons; Parental transmission; Fish early-life stages; PhotoMotor Response; Cardiac performance; PAH metabolites; Genotoxicity. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00259/37046/37174.pdf |
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| Le Bihanic, Florane; Clerandeau, Christelle; Le Menach, Karyn; Morin, Benedicte; Budzinski, Helene; Cousin, Xavier; Cachot, Jerome. |
| In aquatic environments, polycyclic aromatic hydrocarbons (PAHs) mostly occur as complex mixtures, for which risk assessment remains problematic. To better understand the effects of PAH mixture toxicity on fish early life stages, this study compared the developmental toxicity of three PAH complex mixtures. These mixtures were extracted from a PAH-contaminated sediment (Seine estuary, France) and two oils (Arabian Light and Erika). For each fraction, artificial sediment was spiked at three different environmental concentrations roughly equivalent to 0.5, 4, and 10 μg total PAH g−1 dw. Japanese medaka embryos were incubated on these PAH-spiked sediments throughout their development, right up until hatching. Several endpoints were recorded at different... |
| Tipo: Text |
Palavras-chave: Developmental abnormalities; Comet assay; Micronucleus assay; Larvae locomotion; Oil extract; Pyrolytic extract; Medaka embryo-larval assay. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00188/29920/28385.pdf |
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| Larcher, Thibaut; Perrichon, Prescilla; Vignet, Caroline; Ledevin, Mireille; Le Menach, Karyn; Lyphout, Laura; Landi, Laure; Clerandeau, Christelle; Lebihanic, F.; Menard, Dominique; Burgeot, Thierry; Budzinski, Helene; Akcha, Farida; Cachot, J.; Cousin, Xavier. |
| Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants that can be present at high levels as mixtures in polluted aquatic environments. Many PAHs are potent mutagens and several are well-known carcinogens. Despite numerous studies on individual compounds, little is known about the toxicity of PAHs mixtures that are encountered in environmental situations. In the present work, zebrafish were continuously fed from 5 days post-fertilisation to 14 months post-fertilisation (mpf) with a diet spiked with fractions of either pyrolytic (PY), petrogenic light oil (LO), or petrogenic heavy oil (HO) origin at three concentrations. A decrease in survival was identified after 3 mpf in fish fed with the highest concentration of HO or LO, but not for PY. All... |
| Tipo: Text |
Palavras-chave: Polycyclic aromatic hydrocarbon; Zebrafish; Carcinogenesis; Genotoxicity; Neoplasia; Carcinoma; Toxicological pathology. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00242/35329/34108.pdf |
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| Burgeot, Thierry; Akcha, Farida; Menard, Dominique; Robinson, Craig; Loizeau, Veronique; Brach-papa, Christophe; Martinez-gomez, Concepcion; Le Goff, Jeremie; Budzinski, Helene; Le Menach, Karine; Cachot, Jerome; Minier, Christophe; Broeg, Katja; Hylland, Ketil. |
| The International workshop on Integrated Assessment of CONtaminants impacts on the North sea (ICON) provided a framework to validate the application of chemical and biological interpretation thresholds (BACs and EACs) in the Seine Bay in France. Bioassays (oyster larval anomalies, Corophium arenarium toxicity assay and DR Calux) for sediment and biomarkers: ethoxyresorufin-O-deethylase (EROD) activity, acetylcholinesterase (AChE) activity, lysosomal membrane stability (LMS), DNA strand breaks using the comet assay, DNA adducts, micronucleus (MN), PAH metabolites, intersex and fish external pathologies were analyzed in four marine sentinel species (Platichthys flesus, Limanda limanda, Mytilus sp. and Nucella lapilus). Polycyclic aromatic hydrocarbons... |
| Tipo: Text |
Palavras-chave: Biomarkers; Bioassays; Chemical contamination; Integrated monitoring in chemistry and biology; Local study; Large geographical scale. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00357/46789/46655.pdf |
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| Gonzalez, Jean-louis; Tapie, Nathalie; Budzinski, Helene; Guyomarch, Julien; Crenn, Vincent; Bretaudeau-sanjuan, Jane. |
| L'objectif principal de cette première étude était d'évaluer, dans le contexte guyanais, l'opérationnalité de techniques d'échantillonnage et de faire une évaluation préliminaire de la contamination chimique de différentes masses d'eaux guyanaises (2 sites en eau de transition et 1 site en eau côtière). Deux types d'échantillonneurs passifs ont été mis en oeuvre: DGT (pour les métaux traces) et SBSE (pour les composés organiques hydrophobes). Les molécules hydrophiles ont été recherchées directement dans l'eau prélevée. Les mesures DGT montrent que le site de l'estuaire de Kourou se caractérise, pour tous les métaux, par des concentrations plus importantes que celles déterminées dans les deux autres stations. S'agissant de mesures ponctuelles, réalisées... |
| Tipo: Text |
Palavras-chave: Guyane; DCE; Contamination chimique; Masses d'eau; Métaux traces; Composés hydrophiles et hydrophobes; Échantillonnage passif; DGT; SBSE. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00035/14623/11902.pdf |
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| Turquet, Jean; Nicet, Jean Benoit; Cambert, Harold; Denis, Yves; Gonzalez, Jean-louis; Bigot, Lionel; Guyomarch, Julien; Budzinski, Helene; Tapie, Nathalie; Jamon, Alban; Pribat, B. |
| Les prochaines échéances imposées par le calendrier de la Directive Cadre sur l’Eau sur les eaux côtières visent à atteindre ou à restaurer le bon état qualitatif et quantitatif de toutes les masses d’eau d'ici l'horizon 2015. Ces objectifs nécessitent la définition du bon état chimique des masses d’eaux, l’évaluation des tendances et la mise en place des programmes de mesures. |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00035/14621/11900.pdf |
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| Munaron, Dominique; Hubert, Morgane; Tapie, Nathalie; Budzinski, Helene; Guyomarch, Julien; Andral, Bruno; Gonzalez, Jean-louis. |
| Since 2008, projects supported by french government agencies were dedicated to improve the assessment of chemical contamination of water bodies defined by the Water Framework Directive (WFD, 2000/60/CE)(Munaron et al, 2012). Among these studies, the aim of PEPSLAG was to investigate the utility of passive samplers for sampling contaminants present at trace levels in coastal lagoons environments, and to realize a first assessment of the contamination on the French Mediterranean coastal lagoons. 23 transitional lagoons water-bodies were investigated along the French Mediterranean coast, using three kinds of passive samplers (DGT, POCIS and SBSE). 141 contaminants from various chemical families (trace metals, pesticides, pharmaceuticals, alkylphenols, PAHs,... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00167/27870/26115.pdf |
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| Gonzalez, Jean-louis; Munaron, Dominique; Podeur, Christian; Tapie, Nathalie; Budzinski, Helene; Marusczak, Nicolas; Montero, Natalia; Belzunce-segarra, Maria Jesus. |
| This project is a contribution to the improvement and development of passive sampling techniques. The objectives are: - to develop ways to limit the fouling formation on the samplers requiring prolonged immersion time (POCIS) and ensure a "constant" and reproducible sampling rate - test and validate the DGT developed specifically for Hg wich is a "WFD priority" metal. Key words (thematic and geographical area) :Passive samplers, POCIS, DGT, fouling, sampling rate, Hg, baie du Lazaret , étang de Thau |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00115/22628/20353.pdf |
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| Registros recuperados: 41 | |
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