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A Red Shrimp, Farfantepenaeus brasiliensis (Latreille, 1817), Larvae Feeding Regime Based on Live Food ArchiMer
Gaxiola, Gabriela; Gallardo, Pedro; Simoes, Nuno; Cuzon, Gerard.
Red shrimp, Farfantepenaeus brasiliensis, larvae's response to different concentrations of live foods (diatoms Chaetoceros gracilis: 20-100 x 103 cells/mL; flagellate Tetraselmis chuii: 2-10 x 103 cells/mL and Artemia nauplii [NA]: 1-5 NA/mL) was investigated in three experiments. Experimental assessments were based on four variables: survival rate, weight gain, development index (DI), and resistance to salinity stress. A combination of C. gracilis 80 x 103 cells/mL, T. chuii 2 x 103 cells/mL, and Artemia 4 NA/mL provided the best experimental response. Specifically, F. brasiliensis larvae fed with the above-mentioned optimal concentrations of microalgae and Artemia grew faster and reached the postlarval stage in less time (168 h) than larvae in other...
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00028/13928/11639.pdf
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Effect of type of binder on growth, digestibility, and energetic balance of Octopus maya ArchiMer
Rosas, C; Tut, J; Baeza, J; Sanchez, A; Sosa, V; Pascual, C; Arena, L; Domingues, P; Cuzon, Gerard.
The present study was designed to test the effects of type of binder on growth, nutritional physiology, total apparent digestibility, and some elements of the energetic balance of early O. maya juveniles. Two experiments were performed. One was aimed at evaluating the effect of type of binder on growth, nutritional physiology, and energetic balance and the other at knowing the effect of the binder on total apparent digestibility of O. maya. Binder type affected growth and survival of early O. maya juveniles. Octopuses fed alginate-bound crab meat lost weight and died, revealing that this type of binder limits nutrient absorption from the diet. In contrast, when gelatin was used as binder, energetic balance was similar to that obtained in animals fed...
Tipo: Text Palavras-chave: Growth rate; Energetic balance; Diet binder; Digestibility; Octopus maya.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3949.pdf
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Substitution of fish meal with plant protein sources and energy budget for white shrimp Litopenaeus vannamei (Boone, 1931) ArchiMer
Suarez, J. A.; Gaxiola, G.; Mendoza, R.; Cadavid, S.; Garcia, G.; Alanis, G.; Suarez, A.; Faillace, J.; Cuzon, Gerard.
Fish meal (FM) has long been considered an essential ingredient for the formulation of shrimp feeds. Until recently, 12%FM was considered a minimum amount, below which weight gain would become reduced due to a reduction in feed intake. In order to test this perceived minimum value, an experiment was conducted on a long-term basis with 4 replicates. Four practical isonitrogenous and isoenergetic diets were formulated for Litopenaeus vannamei juveniles. Survival, weight gain, feed conversion ratio (FCR) and protein efficiency ratio were determined for 0, 6, 10 and 15% FM diets and a reference diet. Shrimp survival ranged from 84 to 86.5%. Shrimp fed a diet with 0% FM showed lower average weight and SGR compared to the other treatments (P<0.05). FCR of...
Tipo: Text Palavras-chave: Metabolism; Growth; Plant proteins; Substitution fish meal; L. vannamei.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6630.pdf
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Glutamate dehydrogenase and Na+-K+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels ArchiMer
Li, Erchao; Arena, Leticia; Lizama, Gabriel; Gaxiola, Gabriela; Cuzon, Gerard; Rosas, Carlos; Chen, Liqiao; Van Wormhoudt, Alain.
Improvement in the osmoregulation capacity via nutritional supplies is vitally important in shrimp aquaculture. The effects of dietary protein levels on the osmoregulation capacity of the Pacific white shrimp (L. vannamei) were investigated. This involved an examination of growth performance, glutamate dehydrogenase (GDH) and Na+-K+ ATPase mRNA expression,, and GDH activity in muscles and gills. Three experimental diets were formulated, containing 25%, 40%, and 50% dietary protein, and fed to the shrimp at a salinity of 25. After 20 days, no significant difference was observed in weight gain, though GDH and Na+-K+ ATPase gene expression and GDH activity increased with higher dietary protein levels. Subsequently, shrimp fed diets with 25% and 50% dietary...
Tipo: Text Palavras-chave: Litopenaeus vannamei; Gene expression; Glutamate dehydrogenase; Na+-K+ ATPase; Protein; Salinity.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00031/14261/11692.pdf
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Immunoreactive human growth hormone like peptides in tropical Penaeids and the effect of dietary hGH on Penaeus vannamei larval development ArchiMer
Toullec, J. Y.; Le Moullac, Gilles; Cuzon, Gerard; Van Wormhoudt, A.
Human growth hormone like peptides were present in Penaeus indicus as two molecular forms: one with a molecular weight about 25 kDa which is similar to that of the vertebrate growth hormone and a smaller form of 2 kDa. Quantitative variations of hGH-like peptides in different larval stages of three species of Penaeids (Penaeus vannamei, P. stylirostris, P. indicus) suggest a possible involvement of hGH-like peptides in the development. Human growth hormone (hGH) supplementation in the diet of Penaeus vannamei larvae seems to have a positive effect on the size and on the quality of the animals estimated by their resistance to salinity stress.
Tipo: Text Palavras-chave: Growth hormone; Growth; P. indicus; P. stylirostris; P. vannamei; Penaeids; Hormone de croissance; Croissance; P. indicus; P. stylirostris; P. vannamei; Pénéides.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/publication-4429.pdf
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Energy balance of Litopenaeus vannamei postlarvae fed on animal or vegetable protein based compounded feeds ArchiMer
Jimenez Yan, L; Brito, A; Cuzon, Gerard; Gaxiola, G; Garcia, T; Taboada, G; Soto, L; Brito, R.
L. vannamei postlarvae are normally raised with a protein dense diet (50% protein) rich in fishmeal. Part of the protein is utilized for energy purpose instead of protein synthesis. Based on a previous energy partitioning study, the effects of two isoenergetic compounded feed treatments - animal protein (AP) and vegetable protein and carbohydrates (VPC) - upon growth efficiency and energy budget of shrimp postlarvae and early juveniles were determined. Recovered energy (RE) or production (P) after 50 days trial was similar (2 J day(-1)) in both treatments, from PL14 to PL19. However, early juveniles discriminated between animal protein (116 J day(-1)) and vegetable protein and carbohydrates (88 J day(-1)). The difference in respiration indicated a higher...
Tipo: Text Palavras-chave: Shrimp; Physiology; Vegetable protein; Animal protein; Energy; Juvenile; Postlarvae.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1879.pdf
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Replacement of Artemia franciscana Nauplii by Microencapsulated Diets: Effect on Development, Digestive Enzymes, and Body Composition of White Shrimp, Litopenaeus vannamei, Larvae ArchiMer
Gallardo, Pedro; Martinez, Gemma; Palomino, Gabriela; Paredes, Adriana; Gaxiola, Gabriela; Cuzon, Gerard; Pedroza-islas, Ruth.
The effects of two microencapsulated feeds were evaluated on development, growth, survival, proteolytic activity, and biochemical composition of white shrimp, Litopenaeus vannamei, larvae. The treatments were: (1) basal microcapsules (BM), (2) microcapsules containing krill hydrolysate (BMK), and (3) live food control (LFC: Artemia franciscana nauplii) with all treatments receiving algae (Chaetoceros ceratosporum and Tetraselmis chuii). No significant differences were found in development index and survival among larvae. Growth rate was significantly higher in larvae fed LFC (15 +/- 0.06%/d) as compared with those offered the BM diet (7.5 +/- 0.5%/d) with the BMK (11 +/- 0.04%/d) treatment producing intermediate results. The activity of total proteases and...
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00136/24774/26979.pdf
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Physiological and biochemical changes of wild and cultivated juvenile pink shrimp Farfantepenaeus duorarum (crustacea: penaeidae) during molt cycle ArchiMer
Luis Bonilla-gomez, Jose; Chiappa-carrara, Xavier; Galindo, Carmen; Jeronimo, Gilberto; Cuzon, Gerard; Gaxiola, Gabriela.
Changes in metabolite levels in hemolymph and hepatopancreas were used as indicators of physiological status of juvenile wild and cultivated Farfantepenaeas. duorarum (Burkenroad, 1939), during hyper-osmoregulatory conditions (salinity 22 ppt, 726 mOsm/kg). We analyzed the relationship between biochemical changes of wild and cultivated shrimp at different molt stages by measurement of osmotic capacity, hemocyanin, acylglycerides, cholesterol, glucose, glycogen and total protein. Biochemical analyses of hemolymph (blood) and hepatopancreas showed a decreasing trend in stages closer to molt and an increasing trend before intermolt. Osmotic capacity and blood proteins indicated a significant effect of the molt stage. Stages A. D1' and D''' showed the greatest...
Tipo: Text Palavras-chave: Biochemical analysis; F. duorarum; Molt stages; Osmoregulation.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00088/19936/17773.pdf
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Reproductive performance, biochemical composition and fatty acid profile of wild-caught and 2nd generation domesticated Farfantepenaeus duorarum (Burkenroad, 1939) broodstock ArchiMer
Emerenciano, Mauricio; Cuzon, Gerard; Mascaro, Maite; Arevalo, Miguel; Norena-barroso, Elsa; Jeronimo, Gilberto; Racotta, Ilie; Gaxiola, Gabriela.
A 30-day trial was performed to evaluate the reproductive performance of wild and 2nd generation domesticated Farfantepenaeus duorarum broodstock. Changes in biochemical composition and fatty acids (FA) profile in the 1st and 4th spawn order females were used as indicators of nutritional condition. Wild population of F. duorarum presented significantly better reproductive outcomes as compared to domesticated ones. Wild spawners achieved significantly higher number of eggs per spawn, number of egg per spawn per g of spawner's body weight and number of nauplii per spawn (P<0.05). Latency period was significantly shorter (P<0.05) for wild than for domesticated females (11 vs 17 days). Wild spawners also presented less mortality (6 vs 12%), higher number...
Tipo: Text Palavras-chave: Fatty acids; F. duorarum; Lipids; Pink shrimp; Nutrition; Reproduction.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00079/19042/17214.pdf
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Testing Protein Digestibility in Red Grouper Epinephelus Morio using In Vitro and In Vivo Methods ArchiMer
Silva, A. F.; Escalante, Karla; Alvarez-gonzalez, C. A.; Guerrero-olazaran, M.; Cuzon, Gerard; Gaxiola, Gabriela.
The digestibility of pre-selected ingredients was evaluated for Epinephelus morio using pH-stat and multi-enzyme extract (stomach, intestine, and ceca). The degree of hydrolysis (DH) was tested with acid and alkaline proteases using crustacean meals, squid meal, fish protein concentrate (FPC), fish meal, dried whey, as well as canola paste (w/or w/o phytase), soybean meal (w/or w/o phytase), soyprotein concentrate (SPC), and wheat gluten. SPC produced DH values between 3.6-4.1%, obtained from the three different parts of the digestive tract. Canola paste produced high DH in the stomach by adding phytase to 0.8 and 2% feed. This process was repeated under alkaline conditions for DH, with 0.6-2.6% in the pyloric ceca, and 1-2.2% in the intestine. The DH from...
Tipo: Text Palavras-chave: ADC; In vitro digestibility; Enzymes; Fish; Feedstuffs.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00248/35930/80189.pdf
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The effects of fish hydrolysate (CPSP) level on Octopus maya (Voss and Solis) diet: Digestive enzyme activity, blood metabolites, and energy balance ArchiMer
Aguila, J; Cuzon, Gerard; Pascual, C; Domingues, P; Gaxiola, G; Sanchez, A; Maldonado, T; Rosas, C.
As has been demonstrated in previous studies, Octopus maya can be fed on artificial diets. In the present study six different diets were assayed. Five diets were designed to test the effect of percentage of inclusion of fish protein concentrate (CPSP: 0, 5, 10, 15, and 20%) and were offered to octopuses as a specifically designed artificial diet. The sixth diet consisted of frozen crab (Callinectes spp) and was used as control diet. Blood metabolites and energy budget of octopuses were evaluated to determine how CPSP levels modulate the digestive capacity and allow retaining energy for growth. Wild animals (316.4 +/- 9.8 g) were used in the study. Results showed that CPSP produced a positive specific growth rate (SGR, % day(-1)) with high value in...
Tipo: Text Palavras-chave: Energy balance; Blood metabolites; Nutrition; Octopus maya.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3638.pdf
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Probiotic effect of FLOC on Vibrios in the pacific white shrimp Litopenaeus Vannamei ArchiMer
Aguilera-rivera, Diana; Prieto-davo, Alejandra; Escalante, Karla; Chavez, Cristina; Cuzon, Gerard; Gaxiola, Gabriela.
The advantages of FLOC over clear water (CW) in rearing juvenile L. vannamei and its effects on Vibrio communities were evaluated. Survival rate in FLOC and clear water were recorded and a probiotic was tested under both conditions. Daily growth rate (DGR) was higher in FLOC (p < 0.05) than in CW. Survival in each system increased significantly when a probiotic was included in the diet (p < 0.05). The Vibrionaceae community from the hepatopancreas (HP) and the culture medium did not differ between the two culture media. Nevertheless, a novel group of Vibrio strains was found to be unique to FLOC. No high level of lesions was observed in shrimp tissues from the FLOC + probiotic treatment; it suggests that the probiotic contributed to homeostasis and...
Tipo: Text Palavras-chave: FLOC; Probiotic; L. vannamei; Disease; Vibrio.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00173/28379/26694.pdf
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The effect of starvation on refeeding, digestive enzyme activity, oxygen consumption, and ammonia excretion in juvenile white shrimp Litopenaeus vannamei ArchiMer
Comoglio, Li; Gaxiola, G; Roque, A; Cuzon, Gerard; Amin, O.
Juveniles of the white shrimp Litopenaeus vannamei were kept Without food for between 0 to 15 clays to evaluate the impact of starvation oil physiologic state (oxygen consumption, poststarvation refeeding, index, nitrogen excretion, and O:N ratio) and digestive enzymes activity. Physiologic changes were found after 6 days of fasting, and refeeding ability declined as a result. Nevertheless. the shrimp were able to Survive 16 days Without food. Starvation Caused metabolism to drop progressively toward a basal level (21 J (.) h(-1 .) g(-1)) and a decrease in the rate of ammonia excretion, because of the catabolism of amino acids front soluble protein in the hepatopancreas. This decrease led to an increase in digestive enzymes specific activity (U/mg...
Tipo: Text Palavras-chave: Digestive enzymes; Litopenaeus vannamei; Metabolic rate; Shrimp; Starvation.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/00000/10877/7911.pdf
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Floc contribution on spawning performance of blue shrimp Litopenaeus stylirostris ArchiMer
Emerenciano, Mauricio; Cuzon, Gerard; Goguenheim, Jean; Gaxiola, Gabriela.
The floc system has been successfully applied for shrimp grow-out, but little is known about floc contribution on reproductive performance. In a 30-day trial, spawning performance of broodstock was evaluated in floc and earthen ponds. Floc spawners achieved better results (P < 0.05) compared with pond spawners in terms of number of spawns per ablated female (2.9 vs. 1.3), spawns per spawning female (3.6 vs. 2.4) and number of consecutive maturation (average of 3.6 times compared with 2.5 times in ponds females). Latency period was lower for floc spawners (7.4 days) than pond spawners (10.7 days) (P < 0.05). The percentage of females that spawned at least once were higher in floc (77.1%) when compared with pond spawners (53.8%) (P < 0.05). Number...
Tipo: Text Palavras-chave: Floc; Litopenaeus stylirostris; Reproduction; Broodstock.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00115/22638/21495.pdf
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Effect of dietary protein level on growth and energy utilization by Litopenaeus stylirostris under laboratory conditions ArchiMer
Gauquelin, F; Cuzon, Gerard; Gaxiola, G; Rosas, C; Arena, L; Bureau, D; Cochard, Jean-claude.
A study was conducted using a bioenergetics approach to generate information on energy requirement and feed utilization of Litopenaeus stylirostris. Animals (initial mean weight 21 +/- 1 g were fed ad libitum six experimental diets, ranging from 25 to 58% crude protein (CP), for 50 days. Weight gain increased from 21 to 30 g with increasing dietary protein level. Survival rates averaged 80%. Basal metabolism (HeE) and heat increment of feeding (HiE) were monitored using respirometry. HeE was on average I U shrimp(-1) day(-1) or 47 U kg live weight(-1) day (22 kJ/kg(0.8). d(-1)), slightly more than what is observed in fish. HiE averaged 0.2 W/sbrimp(-1) day(-1) or 10 W kg live weight(-1) day(-1) (4 kJ/kg(0.8) d(-1)). It represented 3 1 % and 12%...
Tipo: Text Palavras-chave: Respiration; Excretion; Growth; Dietary protein; L. stylirostris.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-6263.pdf
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Performance of Penaeus stylirostris After Six Generations of Selection for Growth ArchiMer
Goyard, Emmanuel; Penet, Laurent; Chim, Liet; Cuzon, Gerard; Bedier, Edouard; Bureau, D..
Tipo: Text
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00174/28479/26819.pdf
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Differential gene expression in Litopenaeus vannamei shrimp in response to diet changes ArchiMer
Chavez-calvillo, G.; Perez-rueda, E.; Lizama, G.; Zuniga Aguilar, J. J.; Gaxiola, G.; Cuzon, Gerard; Arena-ortiz, L..
We evaluated changes in gene expression of Litopenaeus vannamei shrimp in response to a change in diet by comparing specimens which were fed with an animal protein diet against those which underwent a change from an animal protein diet to a plant protein diet. In addition, we also compared specimens which were fed with a plant protein diet with another group which underwent a change from plant to animal protein diet. Results showed that, in the case of hepatopancreas tissue, a total of six genes were differentially expressed and exhibited a high degree of homology with sequences which codify for proteins related to metabolism, transcription regulation, and rRNA subunits. In the case of muscle tissue, four differentially expressed sequences were recorded...
Tipo: Text Palavras-chave: Differential display; RT-PCR; Litopenaeus vannamei; Protein diet; Alternative protein sources.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00002/11298/7853.pdf
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Is it possible to raise, offspring of the 25th generation of Litopenaeus vannamei (Boone) and 18th generation Litopenaeus stylirostris (Stimpson) in clear water to 40 g? ArchiMer
Cuzon, Gerard; Arena, Leticia; Goguenheim, Jean; Goyard, Emmanuel.
Peneid shrimp are commonly reared in clear-water aquaria or tanks for short-term studies of 4-6 weeks during controlled experiments, such as nutrition studies to estimate dietary nutrient requirements. Recently, in line with the genetic program at Centre Oceanologique du Pacifique (COP), experimental clear-water facilities were tested for the first time over a longer rearing period. Environmental conditions used to maintain animal growth in clear-water system using a well-balanced diet were defined. After 100 days, shrimp growth rates tended to decrease and only by adjusting the diet could shrimp complete their life cycle and reach maturation size of around 40 g. This paper presents information on stocking density, diet quality and husbandry linked to...
Tipo: Text Palavras-chave: Formulated diets; Husbandry; Quarantine; Litopenaeus stylirostris; Shrimp; Nutrition; Zootechnie; Quarantaine; Litopenaeus stylirostris; Crevette.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-619.pdf
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Nutrition of the seabass Lates calcarifer ArchiMer
Cuzon, Gerard; Chou, R; Fuchs, Jacques.
The Primary Production Department of Singapore (PPD) studies covered the weaning of seabass from fingerlings stage onwards and the dietary protein requirement of early grow-out fish. Dietary protein requirement of early grow-out seabass was demonstrated to be between 40-50%, at dietary lipid level of 12%, using fishmeal protein. Nevertheless fish growth was significantly higher with trashfish feed, but apparent protein retention was significantly better with formulated feed.
Tipo: Text Palavras-chave: Lates calcarifer; Fry; Nutritional requirements; Biochemistry; Food absorption; Growth rate; Fish meal; Seed (aquaculture); Dietary deficiencies; Fingerlings; Lipids; Proteins; Diets; Pellet feeds.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1425.pdf
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Physiological and Biochemical Variations during the Molt Cycle in Juvenile Litopenaeus vannamei under Laboratory Conditions ArchiMer
Galindo, Carmen; Gaxiola, Gabriela; Cuzon, Gerard; Chiappa-carrara, Xavier.
Biochemical changes, specifically in hemocyanin and glucose concentrations in hemolymph, glycogen in the digestive gland. were studied throughout the molt cycle in juveniles of Litopenaeus vannamei in a high salinity condition (36), and related to variations in the osmotic capacity of organisms. Increasing hemolymph volume before molting modifies circulating glucose and hemocyanin concentrations acting on the osmotic capacity. Variations in glucose concentrations are indicators of differential energy requirements throughout the molt cycle. Hemocyanin was used as an indirect indicator of the energy consumed by shrimp in various molt processes that affect the osmotic capacity. The co-variability of these metabolites is the result of the biochemical...
Tipo: Text Palavras-chave: Molt cycle; Litopenaeus vannamei; Hypo osmotic conditions; Biochemistry.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6899.pdf
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