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	Provedor de dados BJM (10) Ciênc. Tecnol. Aliment. (4) BABT (3) Rev. Microbiol. (2) Rev. Bras. Bot. (1) Autor Gomes,Eleni (20) Silva,Roberto da (15) Lago-Vanzela,Ellen Silva (3) Martin,Natalia (3) Martins,Gisele Marta (2) Pagnocca,Fernando Carlos (2) Silva,Dênis (2) Souza,Simone Regina de (2) Abrahão,Maira Cortellini (1) Aguiar,Ana Paula (1) Bezzerra-Bussoli,Carolina (1) Blandino,Ana (1) Bocchini-Martins,Daniela A. (1) Bocchini-Martins,Daniela Alonso (1) Bonfá,Maricy Raquel B. (1) Boscolo,Mauricio (1) Boscolo,Maurício (1) Caro,Ildefonso (1) Carvalho,Ana Flávia Azevedo (1) Carvalho,Caio César (1) Mais... Palavra-chave Glucoamylase (3) Polygalacturonase (3) Aspergillus (2) Ethanol (2) Solid-state fermentation (2) Xylanase (2) Xylose (2) Agaricales (1) Agricultural residues (1) Alpha-glucosidase (1) Antocianina (1) Análise sensorial (1) Aspergillus awamori (1) Aspergillus sp (1) Bacillus sp (1) Basidiomycetes (1) By-product (1) CMCase (1) Cajá-manga (1) Cajá-manga pulp (1) Mais... Tipo do documento Info:eu-repo/semantics/article (20) Ano 2018 (1) 2016 (2) 2012 (1) 2011 (4) 2009 (2) 2008 (2) 2006 (2) 2005 (2) 2004 (1) 2002 (1) 1999 (1) 1998 (1) Mais... País Brazil (20) Idioma Inglês (18) Português (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 20 Primeira ... 1 ... Última Selection of Xilose-Fermenting Yeast Strains BABT Silva,Rosimeire Oenning da; Cereda,Marney Pascoli; Gomes,Eleni; Martins,Gisele Marta; Pagnocca,Fernando Carlos; Silva,Roberto da. ABSTRACT In Brazil, ethanol is obtained by fermentat of sugar cane juice using Saccharomyces cerevisiae. The cane juice extraction generates the bagasse that has been used for obtaining generation biofuel. However, the sugarcane bagasse has 30% pentose that cannot be fermented to ethanol by S. cerevisiae. Thus the aim of this study was to isolate a yeast able to ferment xylose to ethanol. Samples of cane juice and flowers were used for the isolation of 165 strains that were then screened for ethanol production using plate testing. Among them, the ethanol positive strains Wickerhamomyces anomalus, Schizosaccharomyces pombe and Starmerella meliponinorum were selected for a xylose fermentation assay, using a semi-synthetic and bagasse hydrolysate as must. S.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylose; Ethanol; Yeast; Sugarcane bagasse.. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100419 Localization and partial characterization of thermostable glucoamylase produced by newly isolated Thermomyces lanuginosus TO3 in submerged fermentation BABT Gonçalves,Aline Zorzetto Lopes; Carvalho,Ana Flávia Azevedo; Silva,Roberto da; Gomes,Eleni. Thermophilic Thermomyces lanuginosus strain TO3 was isolated from compost pile samples and was used for its ability to produce considerable glucoamylase activity when growing in liquid medium at 45ºC with starch as the sole carbon source. Enzyme productivity was high in submerged fermentation (SmF) with maximum activity of 13 U/mL after 168 h of fermentation. Higher quantities of glucose were released when the substrate for enzyme was soluble starch than maltose or maltooligosaccharides were used. The distribution of glucoamylase between the extracellular and cell-associated fractions varied according to fermentation time. Glucoamylase produced from T. lanuginosus TO3 had optimum activity at 65 ºC and good thermostability in the absence of substrate, with... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucoamylase; Submerged fermentation; Thermomyces lanuginosus. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400024 Pectinase production by fungal strains in solid-state fermentation using agro-industrial bioproduct BABT Martin,Natalia; Souza,Simone Regina de; Silva,Roberto da; Gomes,Eleni. Pectin lyase and polygalacturonase production by newly isolated fungal strains was carried out in solid-state fermentation. Moniliella SB9 and Penicillium sp EGC5 produced polygalcturonase (PG) and pectin lyase (PL) on mixture of orange bagasse, sugar cane bagasse and wheat bran as substrate. PG and PL produced by Moniliella presented optimum activity at pH 4.5 and 10.0 and at 55 and 45°C, respectively, while these enzymes from Penicillium sp presented optimum activity at pH 4.5-5.0 and 9.0 and 40°C, respectively. Tipo: Info:eu-repo/semantics/article Palavras-chave: Penicillium; Moniliella; Polygalacturonase; Pectin lyase. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500018 The isolation of pentose-assimilating yeasts and their xylose fermentation potential BJM Martins,Gisele Marta; Bocchini-Martins,Daniela Alonso; Bezzerra-Bussoli,Carolina; Pagnocca,Fernando Carlos; Boscolo,Maurício; Monteiro,Diego Alves; Silva,Roberto da; Gomes,Eleni. ABSTRACT For the implementation of cellulosic ethanol technology, the maximum use of lignocellulosic materials is important to increase efficiency and to reduce costs. In this context, appropriate use of the pentose released by hemicellulose hydrolysis could improve de economic viability of this process. Since the Saccharomyces cerevisiae is unable to ferment the pentose, the search for pentose-fermenting microorganisms could be an alternative. In this work, the isolation of yeast strains from decaying vegetal materials, flowers, fruits and insects and their application for assimilation and alcoholic fermentation of xylose were carried out. From a total of 30 isolated strains, 12 were able to assimilate 30 g L-1 of xylose in 120 h. The strain Candida... Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylose; Yeasts; Ethanol. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100162 A protein expression system for tandem affinity purification in Xanthomonas citri subsp. citri BJM Dantas,Giordanni C.; Martins,Paula M.M.; Martins,Daniela A.B.; Gomes,Eleni; Ferreira,Henrique. Abstract Citrus canker, caused by the Gram-negative bacterium Xanthomonas citri subsp. citri (Xac), is one of the most devastating diseases to affect citrus crops. There is no treatment for citrus canker; effective control against the spread of Xac is usually achieved by the elimination of affected plants along with that of asymptomatic neighbors. An in depth understanding of the pathogen is the keystone for understanding of the disease; to this effect we are committed to the development of strategies to ease the study of Xac. Genome sequencing and annotation of Xac revealed that ∼37% of the genome is composed of hypothetical ORFs. To start a systematic characterization of novel factors encoded by Xac, we constructed integrative-vectors for protein... Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus canker; Expression vectors; TAP-tag. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200518 Production of xylanase and CMCase on solid state fermentation in different residues by Thermoascus aurantiacus miehe BJM Silva,Roberto da; Lago,Ellen S.; Merheb,Carolina W.; Macchione,Mariana M.; Park,Yong Kun; Gomes,Eleni. The use of waste as raw material is important for government economy and natural balance. The purpose of this work was to study the production of CMCase and xylanase by a Brazilian strain of Thermoascus aurantiacus in solid state fermentation (SSF) using different agricultural residues (wheat bran, sugarcane bagasse, orange bagasse, corncob, green grass, dried grass, sawdust and corn straw) as substrates without enrichment of the medium and characterize the crude enzymes.The study of the extracellular cellulolytic and hemicellulolytic enzymes showed that T. arantiacus is more xylanolytic than cellulolytic. The highest levels of enzymes were produced in corncob, grasses and corn straw. All the enzymes were stable at room temperature by 24 h over a broad pH... Tipo: Info:eu-repo/semantics/article Palavras-chave: Thermoascus aurantiacus; Xylanase; CMCase; Solid state fermentation; Agricultural residues. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300006 Xylanase production by Aspergillus awamori under solid state fermentation conditions on tomato pomace BJM Umsza-Guez,Marcelo A.; Díaz,Ana B.; Ory,Ignacio de; Blandino,Ana; Gomes,Eleni; Caro,Ildefonso. In this work, tomato pomace, a waste abundantly available in the Mediterranean and other temperate climates agro-food industries, has been used as raw material for the production of some hydrolytic enzymes, including xylanase, exo-polygalacturonase (exo-PG), cellulase (CMCase) and α-amylase. The principal step of the process is the solid state fermentation (SSF) of this residue by Aspergillus awamori. In several laboratory experiments, maximum xylanase and exo-PG activities were measured during the first days of culture, reaching values around 100 and 80 IU/gds (international units of enzyme activity per gram of dried solid), respectively. For CMCase and α-amylase production remained almost constant along fermentation, with average values of 19 and 21.5... Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid state fermentation (SSF); Tomato pomace; Hydrolytic enzymes; Plate-type bioreactor; Xylanase production. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400046 Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation BJM Moretti,Marcia M. S.; Bocchini-Martins,Daniela A.; Silva,Roberto Da; Rodrigues,André; Sette,Lara D.; Gomes,Eleni. Twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45ºC on medium containing corn straw and cardboard as carbon sources. These fungi were identified in the genera Aspergillus, Thermomyces, Myceliophthora, Thermomucor and Candida. The majority of the isolated strains produced xylanase and cellulases under solid state fermentation (SSF). The highest cellulase and xylanase productions were obtained by the cultivation of the strains identified as Aspergillus fumigatus M.7.1 and Myceliophthora thermophila M.7.7. The enzymes from these strains exhibited maximum activity at pH 5.0 and at 60 and 70ºC. The endo-glucanase from A. fumigatus was stable from... Tipo: Info:eu-repo/semantics/article Palavras-chave: Thermophilic fungi; Solid-state fermentation; Xylanase; Cellulase. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300032 Production and characterization of glucoamylase from fungus Aspergillus awamori expressed in yeast Saccharomyces cerevisiae using different carbon sources BJM Pavezzi,Fabiana Carina; Gomes,Eleni; Silva,Roberto da. Glucoamylase is widely used in the food industry to produce high glucose syrup, and also in fermentation processes for production beer and ethanol. In this work the productivity of the glucoamylase of Aspergillus awamori expressed by the yeast Saccharomyces cerevisiae, produced in submerged fermentation using different starches, was evaluated and characterized physico-chemically. The enzyme presented high specific activity, 13.8 U/mgprotein or 2.9 U/mgbiomass, after 48 h of fermentation using soluble starch as substrate. Glucoamylase presented optimum activity at temperature of 55ºC, and, in the substratum absence, the thermostability was for 1h at 50ºC. The optimum pH of activity was pH 3.5 - 4.0 and the pH stability between 5.0 and 7.0. The half life at... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucoamylase; Aspergillus awamori; Saccharomyces cerevisiae; Corn starch; Potato starch; Cassava starch. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100024 Ligninases production by Basidiomycetes strains on lignocellulosic agricultural residues and their application in the decolorization of synthetic dyes BJM Gomes,Eleni; Aguiar,Ana Paula; Carvalho,Caio César; Bonfá,Maricy Raquel B.; Silva,Roberto da; Boscolo,Mauricio. Wood rotting Basidiomycetes collected in the "Estação Ecológica do Noroeste Paulista", São José do Rio Preto, São Paulo State, Brazil, concerning Aphyllophorales order and identified as Coriolopsis byrsina SXS16, Lentinus strigellus SXS355, Lentinus sp SXS48, Picnoporus sanguineus SXS 43 and Phellinus rimosus SXS47 were tested for ligninases production by solid state fermentation (SSF) using wheat bran or rice straw as culture media. C. byrsina produced the highest laccase (200 U mL-1) and Lentinus sp produced the highest activities of manganese peroxidase (MnP) and lignin peroxidase (LiP) (7 and 8 U mL-1, respectively), when cultivated on wheat bran. The effect of N addition on enzyme production was studied in medium containing rice straw and the data... Tipo: Info:eu-repo/semantics/article Palavras-chave: Laccase; Manganese peroxidase; Lignin peroxidase; Basidiomycetes; Decolorization; Synthetic dye. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100005 Production of thermostable glucoamylase by newly isolated Aspergillus flavus A 1.1 and Thermomyces lanuginosus A 13.37 BJM Gomes,Eleni; Souza,Simone Regina de; Grandi,Roseli Picolo; Silva,Roberto da. Thirteen thermophilic fungal strains were isolated from agricultural soil, tubers and compost samples in tropical Brazil. Two strains were selected based on of their ability to produce considerable glucoamylase activity while growing in liquid medium at 45ºC with starch as the only carbon source. They were identified as Aspergillus flavus A1.1 and Thermomyces lanuginosus A 13.37 Tsiklinsky. The experiment to evaluate the effect of carbon source, temperature and initial pH of the medium on enzyme production was developed in a full factorial design (2x2x3). Enzyme productivity was influenced by the type of starch used as carbon source. Cassava starch showed to be a better substrate than corn starch for glucoamylase production by A. flavus but for T.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucoamylase; Alpha-glucosidase; Aspergillus; Thermomyces. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100015 Production and partial characterization of polygalacturonases produced by thermophilic Monascus sp N8 and by thermotolerant Aspergillus sp N12 on solid-state fermentation BJM Freitas,Paula Mendes de; Martin,Natalia; Silva,Dênis; Silva,Roberto da; Gomes,Eleni. Polygalacturonases production by newly isolated Monascus sp N8 and Aspergillus sp N12 strains was carried out in solid-state fermentation using mixtures of wheat bran, sugar cane bagasse and orange bagasse as carbon sources. The maximal activity values of exo-polygalacturonases (exo-Pg) from Monascus sp and Aspergillus sp were obtained using wheat bran/sugar cane bagasse/orange bagasse mixture (6.6 U/mL) and wheat bran/orange bagasse mixture (10 U/mL), respectively. Enzyme production by both strains was higher at 45ºC after 72 h and 1.6 U/mL at 50ºC after 120 h. Endo-polygalacturonase (endo-Pg) production was higher in wheat bran/orange bagasse mixture and was not affected by temperature of incubation for both fungi. Endo-Pg production by Monascus was 1.8... Tipo: Info:eu-repo/semantics/article Palavras-chave: Monascus sp; Aspergillus sp; Thermophilic; Polygalacturonase; Solid-state fermentation. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300019 Pectinase production by Penicillium viridicatum RFC3 by solid state fermentation using agricultural wastes and agro-industrial by-products BJM Silva,Dênis; Martins,Eduardo da Silva; Silva,Roberto da; Gomes,Eleni. Pectin lyase and polygalacturonase production by newly isolated Penicillium viridicatum strain Rfc3 was carried out by means of solid state fermentation using orange bagasse, corn tegument, wheat bran and mango and banana peels as carbon sources. The maximal activity value of polygalacturonase (Pg) (30U.g-1) was obtained using wheat bran as carbon source while maximal pectin lyase (Pl) (2000 U.g-1) activity value was obtained in medium composed of orange bagasse. Mixtures of banana or mango peels with sugar cane bagasse resulted in increased Pg and Pl production compared to fermentations in which this residue was not used. The mixture of orange bagasse and wheat bran (50%) increased the production of Pg and Pl to 55 U.g-1 and 3540 U.g -1 respectively.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Penicillium viridicatum polygalacturonase pectin lyase wastes; By-product. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400008 Physical-chemical, caloric and sensory characterization of light jambolan (Syzygium cumini Lamarck) jelly Ciênc. Tecnol. Aliment. Lago-Vanzela,Ellen Silva; Santos,Ginaldo Vieira dos; Lima,Fernanda Arcaro de; Gomes,Eleni; da Silva,Roberto. In Brazil, several little economically explored fruits have good potential as raw material for the agro-industry. This study aimed to produce and determine the physical-chemical and sensory characteristics of light jambolan jelly. This fruit has intense purple color, which gave the jellies - both standard and light - a quite attractive visual aspect. The light jellies exhibited similar physical-chemical characteristics to the ones developed through the conventional method and; with the proportion of sweeteners used, the caloric values of the formulations were reduced to the range of 41 to 53%, attending the requirements of the Brazilian legislation for this type of product. The sensory profile obtained for the 4 light formulations developed, clearly showed... Tipo: Info:eu-repo/semantics/article Palavras-chave: Jambolan; Light jelly; Sweetener. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000300018 Produção de geléia de jambolão (Syzygium cumini Lamarck): processamento, parâmetros físico - químicos e avaliação sensorial Ciênc. Tecnol. Aliment. Lago,Ellen Silva; Gomes,Eleni; Silva,Roberto da. A fruta jambolão (Syzygium cumini Lamarck) é uma fruta de coloração roxa intensa e sabor agradável. Dado que não há na literatura nenhum relato de seu aproveitamento industrial, a produção de geléia de jambolão tornou-se uma interessante atividade de pesquisa. Este trabalho objetivou a elaboração e a avaliação das características físico-químicas e sensoriais da geléia obtida do jambolão. A fruta apresentou a seguinte composição química: cinzas, 0,34%; lipídeos, 0,30%; proteínas, 0,67%; carboidratos, 10,07%; fibras, 0,28%; umidade, 87,75%; frutose, 0,4%; glicose, 0,6%; antocianinas totais, 0,276%; substâncias pécticas, 0,245%; acidez titulável, 5,91%; sólidos solúveis, 9,00%; e pH, 3,9. A geléia obtida apresentou a seguinte composição: açúcares redutores,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Jambolão; Geléia; Antocianina; Análise sensorial. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612006000400021 Effect of pectinolitic enzymes on the physical properties of caja-manga (Spondias cytherea Sonn.) pulp Ciênc. Tecnol. Aliment. Umsza-Guez,Marcelo Andres; Rinaldi,Rebeca; Lago-Vanzela,Ellen Silva; Martin,Natalia; Silva,Roberto da; Gomes,Eleni; Thoméo,João Cláudio. Cajá-manga, also known as golden apple and hog-plum, is an exotic fruit native from Îles de la Société (French Polynesia), which was first introduced in Brazil in 1985. The pulp of ripe fruit was treated with the commercial enzymatic pool and its effect was evaluated in terms of yield, as well as the physical properties viscosity, turbidity and color (L* values). Response surface methodology was used and three levels were adopted for the independent variables temperature (30, 40, and 50 ºC), incubation time (30, 60 and 90 minutes) and enzyme concentration (0.01, 0.05, 0.09 v/v%). A central composite statistical design was used to guide the experimental work. The enzyme treatment highly increased both juice yield (up to 56%) and color (up to 8.6%) and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pectinolytic enzymes; Pectinex Ultra SP; Cajá-manga pulp; Enzyme treatment; Juice extraction; Juice viscosity. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000200037 Chemical and sensory characteristics of pulp and peel 'cajá-manga' (Spondias cytherea Sonn.) jelly Ciênc. Tecnol. Aliment. Lago-Vanzela,Ellen Silva; Ramin,Priscila; Umsza-Guez,Marcelo Andrés; Santos,Ginaldo Vieira; Gomes,Eleni; Silva,Roberto da. Cajá-manga (Spondias cytherea) is a fruit with intense aroma and a sweet sour taste, thus being very appreciated. Its peel, in spite of being more fibrous, is tender and many times consumed with the pulp. The peel is a part of foods normally discarded, but many times, it is rich in taste and fibers, representing an option at the elaboration of new foods. Since there are no reports in the literature on the use of cajá-manga peel for jelly making, this work had the purpose of developing and evaluating this product. Based on the results, it was noted that the peels presented higher contents of protein, lipids, ashes, food fiber, total carbohydrates and pectin, and lower content of moisture, when compared to the pulp. The peel and the pulp (control) jellies... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cajá-manga; Jelly; Fruit's peel. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000200018 Poliporóides (Basidiomycota) em fragmentos de mata no perímetro urbano de São José do Rio Preto, São Paulo, Brasil Rev. Bras. Bot. Abrahão,Maira Cortellini; Gugliotta,Adriana De Mello; Gomes,Eleni. Foi realizado levantamento das espécies de fungos poliporóides que ocorrem no perímetro urbano do município de São José do Rio Preto (20º49'12" S, 49º22'44" W), Estado de São Paulo, Brasil. Foram identificadas 18 espécies pertencentes a 11 gêneros de Agaricales (Schizophyllaceae), Hymenochaetales (Hymenochaetaceae) e Polyporales (Ganodermataceae, Gloeophyllaceae, Meruliaceae e Polyporaceae): Coriolopsis floccosa (Jungh.) Ryvarden, C. polyzona (Pers.) Ryvarden, Datronia caperata (Berk.) Ryvarden, D. mollis (Somf.:Fr.) Donk., Ganoderma australe (Fr.) Pat., Gloeophyllum striatum (Sw.) Murrill, Gloeoporus thelephoroides (Hook.) G. Cunn., Hexagonia hirta (P. Beauv.) Fr., H. hydnoides (Sw.) M. Fidalgo, H. papyracea Berk., Polyporus tenuiculus (P. Beauv.) Fr.,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Agaricales; Hymenochaetales; Polyporales; Taxonomia. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042009000300004 Screening of bacterial strains for pectinolytic activity: characterization of the polygalacturonase produced by Bacillus sp Rev. Microbiol. Soares,Márcia M.C.N.; Silva,Roberto da; Gomes,Eleni. One hundred sixty eight bacterial strains, isolated from soil and samples of vegetable in decomposition, were screened for the use of citrus pectin as the sole carbon source. 102 were positive for pectinase depolymerization in assay plates as evidenced by clear hydrolization halos. Among them, 30% presented considerable pectinolytic activity. The cultivation of these strains by submerged and semi-solid fermentation for polygalacturonase production indicated that five strains of Bacillus sp produced high quantities of the enzyme. The physico-chemical characteristics, such as optimum pH of 6.0 - 7.0, optimum temperatures between 45oC and 55oC, stability at temperatures above 40oC and in neutral and alkaline pH, were determined. Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus sp; Polygalacturonase; Pectinolytic activity. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400002 Ribonuclease Production by Aspergillus species Rev. Microbiol. Gomes,Eleni; Silva,Roberto da; Serzedello,Alcides. Ribonuclease production by Aspergillus flavipes, A. sulphureus and A. fischeri in semi-synthetic medium, after 24-144 hours at 30ºC under shaking, was studied. After cultivation, the medium was separated from micelia by filtration and the resultant solution was used as enzymatic extract. The highest amount of biomass and RNase was obtained after 96 hours of cultivation. The enzymes produced by three species presented similar characteristics, with optimum temperature at 55ºC and two peaks of activity at pH 4.5 and 7.0. A. flavipes RNases were more sensitive to temperature: 50% of the initial activity was lost after 1 hour at 70ºC. After this heat treatment, RNase of A. sulphureus lost 30% of this activity and that of A. fischeri only 16%. The nucleotides... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ribonucleases; Aspergillus; Nucleotides. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300008 Registros recuperados: 20 Primeira ... 1 ... Última

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