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| Registros recuperados: 41 | |
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| Suquet, Marc; Agogue, Julie; Brizard, Raphael; Haffray, Pierrick; Labbe, Catherine; Le Coz, Jean-rene; Pepin, Jean-francois; Rault, Paul; Saulnier, Denis. |
| Ce rapport fait état d'une étude réalisée au laboratoire de Génétique et Pathologie de l'IFREMER dans le cadre de l'analyse d'épisodes de mortalité anormale survenue en écloserie privée de crevettes pénéides. Plusieurs hypothèses ont été avancées. La présence de Vibrio harveyi a amené à constituer une collection de 40 souches à partir desquelles une identification bactériologique a été effectuée. Elle montre en effet une dominance de souches appartenant à un groupe homogène de Vibrio harveyi. La technique de ribotypage (digestion d'ADN génomique, transfert de Southem-blot et hybridation avec une sonde spécifique) est alors mise en place. Plusieurs difficultés techniques ont ralenti l'analyse et n'ont donc pas permis d'avoir des résultats assez avancés pour... |
| Tipo: Text |
Palavras-chave: Huître creuse; Crassostrea gigas; Tétraploïdes; Sperme; Conservation; Diagnostic zoo-sanitaire; Flore bactérienne; Herpès virus.. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/00304/41554/40762.pdf |
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| Haffray, Pierrick; Vandeputte, Marc; Petit, V.; Pincent, Cedric; Chatain, Beatrice; Chapuis, H.; Meriaux, J. C.; Coudurier, B.; Quillet, E.; Dupont-nivet, M.. |
| Application of DNA parentage assignment for commercial selection in aquaculture is still rare. An experiment was performed to quantify and to minimize maternal effects on growth in rainbow trout. Six hundred families were reared until 198 day post fertilization (dpf) according to two different procedures. In the first procedure (NORM), all families were pooled at eyed stage. In the second procedure (MIN), where maternal effects due to differences in egg size were expected to be minimal, the spawns were divided into sub-groups with similar mean egg weight at eyed stage. These sub-groups were then pooled when they achieved the same mean body length (147 dpf). Genetic parameters were estimated for body weight, body length and condition factor at 198 dpf, with... |
| Tipo: Text |
Palavras-chave: Rainbow trout; Fingerprint; Maternal effect; Heritability; Growth; Triploid. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00115/22639/20395.pdf |
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| Vandeputte, Marc; Puledda, Antonio; Tyran, Anne Sophie; Bestin, Anastasia; Coulombet, Celine; Bajek, Aline; Baldit, Gwenaelle; Vergnet, Alain; Allal, Francois; Bugeon, Jerome; Haffray, Pierrick. |
| Genetic parameters for carcass and fillet percentage were estimated in 760 European sea bass reared under commercial conditions and slaughtered at 573 days post fertilization (395 g mean body weight). Phenotyped fish were the offspring of 45 sires and 20 dams crossed in a factorial mating design. Pedigrees were re-constructed with 90.7% success using 12 microsatellites. The heritability of fillet yield was moderately low (0.21), while it was high for carcass yield (0.57). Both traits were poorly correlated (− 0.01 to 0.28) making space for their combined improvement. We investigated different predictors derived from measurement of surfaces on digital pictures and ultrasound measurements at several points of the body. The accuracy of the phenotypic... |
| Tipo: Text |
Palavras-chave: Ultrasound tomography; Genetics; Heritability; Indirect selection; Processing yields. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00361/47217/47173.pdf |
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| Labbe, Catherine; Haffray, Pierrick; Mingant, Christian; Quittet, Benjamin; Diss, Blandine; Tervit, H. Robin; Adams, Serean L.; Rimond, Flore; Suquet, Marc. |
| Pacific oyster Crassostrea gigas is one major species for aquaculture, and the development of breeding programs and the need for preservation of wild stock genetic resources prompted the need for larvae cryopreservation. The objective of the present study was to choose the most reliable protocol from several existing publications, to test its biological and practical limitations, and to adapt it to hatchery conditions. The selected protocol was characterized by a very slow freezing rate without seeding, and by the use of ethylene glycol and sucrose as cryoprotectant. The best survivals after thawing and rearing up to 48 h post fertilization (hpf) were obtained with larvae that were frozen at late trochophore (20 hpf) and early-D (24 hpf) stages. Increasing... |
| Tipo: Text |
Palavras-chave: Oyster; Cryobanking; D stage; Ethylene glycol; High throughput; Hatchery. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00423/53460/54354.pdf |
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| Vandeputte, Marc; Garouste, Romain; Dupont-nivet, Mathilde; Haffray, Pierrick; Vergnet, Alain; Chavanne, Herve; Laureau, Stanislas; Ron, Tetsuzan Benny; Pagelson, Glen; Mazorra, Carlos; Ricoux, Remi; Marques, Pedro; Gameiro, Marta; Chatain, Beatrice. |
| Sea bass is a major species in Mediterranean aquaculture, but has a distribution area ranging from North Atlantic to South Mediterranean, with a population structure previously revealed by population genetics. To test the farming performances of wild sea bass populations, we produced a partial diallel cross mating scheme, using sires originating from North Atlantic (NAT), South Atlantic (SAT), West Mediterranean (WEM), North-East Mediterranean (NEM) and South-East Mediterranean (SEM). Fifteen sires per origin were mated in a full-factorial design using artificial fertilization with 9 NAT dams and 17 WEM dams, producing 10 population crosses and 1950 potential full-sib families. All fish were reared together, then tagged at an average weight of 20 g and... |
| Tipo: Text |
Palavras-chave: European sea bass; Dicentrarchus labrax; Selective breeding; Growth; Processing yields; Genotype by environment interaction. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00171/28265/26514.pdf |
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| Prchal, Martin; Kocour, Martin; Vandeputte, Marc; Kause, Antti; Vergnet, Alain; Zhao, Jinfeng; Gela, David; Kašpar, Vojtěch; Genestout, Lucie; Bestin, Anastasia; Haffray, Pierrick; Bugeon, Jérôme. |
| Slaughter yields are traits of high interest especially for fish species sold in processed form like headless carcass or fillets, as well as with regard to increasing consumer preference for easy-to-prepare fish products. However, slaughter yields cannot be measured on live fish and thus their genetic improvement through mass selection is impossible. The usual alternatives are sib selection and/or indirect selection on correlated traits or morphological predictors of slaughter yields. In the present study, we assessed the possibility of using a combination of 3D digitized landmarks and ultrasound measurements in genetic improvement of slaughter yields in common carp. DNA – pedigreed market-size carp (n = 1553 fish) were produced from a partial factorial... |
| Tipo: Text |
Palavras-chave: Fillet yields; 3D predictors; Genetic parameters; Indirect selection; Breeding program. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00604/71625/70070.pdf |
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| Labbe, Catherine; Suquet, Marc; Michel, Gildas; Duclos, Delphine; Lemarchand, Alain; Le Hellec, Catherine; Vandeputte, Marc; Quittet, Benjamin; Goardon, Lionel; Pincent, Cedric; Haffray, Pierrick. |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00099/20979/18602.pdf |
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| Fauvel, Christian; Boryshpolets, Sergey; Cosson, J.; Leedy, J. G. Wilson; Labbe, C.; Haffray, Pierrick; Suquet, Marc. |
| The sperm of seabass is very fragile and it quickly loses its ability to fertilize after collection either if kept undiluted or in classic saline media. In order to avoid cryopreservation when only short conservation is required, the process of sperm management including sperm collection, sperm dilution rate in storage medium and storage medium composition, was subject to experimental trials. A concentration of 20% urine generated a low pH of seminal fluid, and it immediately altered the motility ability. However, pH did not seem to be the key agent of motility prevention since sperm dilution in Leibovitz culture medium (L15) or in classic saline medium both presenting a similar low pH (7.3) did not affect motility. L15 increased the duration of sperm... |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00111/22221/20066.pdf |
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| Peruzzi, Stefano; Wiborg-dahle, Stine; Haffray, Pierrick; Chatain, Beatrice; Primicerio, Raul. |
| A method of geometric morphometrics was used to characterize body shape variation in meiotic gynogenetic and triploid sea bass in comparison with diploid controls. A total of 13 coordinate landmark data were recorded on the profile of 34-month-old control (n = 81), gynogenetic (n = 117) and triploid (n = 131) siblings generated from two females (A and B). Three main axes of morphometric variation were consistent, and these accounted for 60.8 and 57.6% of the total variability in females A and B, respectively. There were significant shape deviations of triploid and gynogenetic fish relative to the controls, and differences were particularly evident in the progeny of one female. Overall, triploids displayed a more slender shape than diploids, whereas... |
| Tipo: Text |
Palavras-chave: Body shape variability; Morphometry; Geometry of Procrustes distance; Triploidy; Gynogenesis; European sea bass; Dicentrarchus labrax. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00025/13651/10757.pdf |
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| Bugeon, Jerome; Lefevre, Florence; Cardinal, Mireille; Uyanik, Ayhan; Davenel, Armel; Haffray, Pierrick. |
| Rainbow trout with different fillet yield [56 and 65% for low (LY) and high yield (HY), respectively] were examined for muscle organization and flesh quality (instrumental and sensorial evaluations). Both groups had similar body weight (3.6 kg in mean), but the HY group had a higher carcass yield (+15%). Higher total muscle area in the HY group (+20%) was associated with a higher number of muscle fibers (+22%). Flesh of the HY group presented a higher area of myosepta (+10%), fat content (+10%) and luminosity (+6%). Raw fillet mechanical resistance was higher for the HY group; an opposite result was obtained for cooked flesh. Sensorial evaluation of cooked flesh revealed no important differences between groups. Smoked fillet from the HY group presented... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00014/12532/9532.pdf |
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| Vandeputte, Marc; Labbe, Catherine; Suquet, Marc; Fauvel, Christian; Goardon, Lionel; Duclos, Delphine; Danchin-burge, Coralie; Vergnet, Alain; Rault, Paul; Degremont, Lionel; Benabdelmouna, Abdellah; Brizard, Raphael; Haffray, Pierrick. |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00098/20975/18597.pdf |
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| Boudry, Pierre; Haffray, Pierrick. |
| The most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) in France to date has been through the breeding of triploids, especially since the development of tetraploids. Triploid oysters commonly resist summer mortality better than diploids. This is most probably because reproductive allocation is so important in this phenomenon. Quantitative genetics studies strongly suggest that a significant gain in resistance to summer mortality, or other traits of interest, could be obtained by selective breeding. Practical difficulties however, and the high cost of breeding large numbers of families under common conditions over many generations remain major constraints to family-based selective breeding in oysters. In the last... |
| Tipo: Text |
Palavras-chave: Polyploidization; Selective breeding; Genetic; Crassostrea gigas; Pacific oysters. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-3438.pdf |
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| Boudry, Pierre; Degremont, Lionel; Taris, Nicolas; Mccombie, Helen; Haffray, Pierrick; Ernande, Bruno. |
| The most significant genetic improvement for production of Pacific oyster (Crassastrea gigas) has been obtained through the breeding of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or for other traits of aquacultural interest, could be obtained using this approach. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have slowed the development of selective breeding programs. Recently, in the USA, Australia and New Zealand, family-based selective breeding programs have been initiated to improve growth and yield. In Europe, where both natural and... |
| Tipo: Text |
Palavras-chave: Microsatellites; Quantitative genetic; Genetic variability; Crassostrea gigas; Oysters. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-2861.pdf |
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| Khanh, Doan Quoc; Vandeputte, Marc; Chatain, Beatrice; Haffray, Pierrick; Vergnet, Alain; Breuil, Gilles; Allal, Francois. |
| Viral Nervous Necrosis (VNN) disease is considered as one of the most serious threats for European sea bass cultured in Mediterranean Sea, with no simple and effective procedures to treat this disease. In this study, 1472 offspring resulting from artificial full factorial mating of western Mediterranean dams with sires from four different wild populations of European sea bass (Northern Atlantic, NAT; Western Mediterranean, WEM; Northern-East Mediterranean, NEM; and Southern-East Mediterranean, SEM) were challenged by experimental infection to W80 betanodavirus strain in order to evaluate genetic variations for VNN resistance among populations and genetic correlations between VNN resistance and production traits. The results showed a large variation of VNN... |
| Tipo: Text |
Palavras-chave: VNN disease; VNN resistance; Heritability; Genetic correlation. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00385/49612/50124.pdf |
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| Smits, Morgan; Enez, Florian; Ferraresso, Serena; Dalla Rovere, Giulia; Vetois, Emilie; Auvray, Jean-françois; Genestout, Lucie; Mahla, Rachid; Arcangeli, Giuseppe; Paillard, Christine; Haffray, Pierrick; Bargelloni, Luca. |
| The Manila clam Ruditapes philippinarum, a major cultured shellfish species, is threatened by infection with the microparasite Perkinsus olseni, whose prevalence increases with high water temperatures. Under the current trend of climate change, the already severe effects of this parasitic infection might rapidly increase the frequency of mass mortality events. Treating infectious diseases in bivalves is notoriously problematic, therefore selective breeding for resistance represents a key strategy for mitigating the negative impact of pathogens. A crucial step in initiating selective breeding is the estimation of genetic parameters for traits of interest, which relies on the ability to record parentage and accurate phenotypes in a large number of... |
| Tipo: Text |
Palavras-chave: Manila clam (Ruditapes philippinarum); Perkinsus olseni infection; Disease resistance; Genetic selection; Genetic parameter estimation. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00658/77031/78319.pdf |
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| Piferrer, Francesc; Beaumont, Andy; Falguiere, Jean-claude; Flajshans, Martin; Haffray, Pierrick; Colombo, Lorenzo. |
| Polyploids can be defined as organisms with one or more additional chromosome sets with respect to the number most frequently found in nature for a given species. Triploids, organisms with three sets of homologous chromosomes, are found spontaneously in both wild and cultured populations and can be easily induced in many commercially relevant species of fish and shellfish. The major consequence of triploidy is gonadal sterility, which is of advantage in the aquaculture of molluscs since it can result in superior growth. In fish, the induction of triploidy is mainly used to avoid problems associated with sexual maturation such as lower growth rates, increased incidence of diseases and deterioration of the organoleptic properties. Triploidy can also be used... |
| Tipo: Text |
Palavras-chave: GMO; Transgenic containment; Genetic containment; Reproductive containment; Hybridisation; Sterility; Fish; Shellfish; Fish farming; Aquaculture; Tetraploidy; Triploidy; Polyploidy. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6648.pdf |
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| Harney, Ewan; Lachambre, Sebastien; Roussel, Sabine; Huchette, Sylvain; Enez, Florian; Morvezen, Romain; Haffray, Pierrick; Boudry, Pierre. |
| Selective breeding strategies require pedigree information over generations, but many species produced in aquaculture are too small to be physically tagged at early stages. Consequently, maintaining a sufficient number of separate families is often needed but costly and logistically difficult. Alternatively, parentage assignment can be obtained using DNA markers. We developed a panel of single nucleotide polymorphism (SNP) markers for the European abalone Haliotis tuberculata using an existing transcriptomic resource. An initial set of 2,176,887 SNPs was filtered to select 500 for high throughput genotyping. Of these, 298 SNPs were amplified in at least 90% of our H. tuberculata samples, consisting of a mixed family cohort (945 offspring) generated by... |
| Tipo: Text |
Palavras-chave: Abalone; Parentage assignment; Pedigree; Selective breeding; Transcriptome; SNP. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00429/54061/55396.pdf |
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| Palaiokostas, Christos; Cariou, Sophie; Bestin, Anastasia; Bruant, Jean-sebastien; Haffray, Pierrick; Morin, Thierry; Cabon, Joelle; Allal, Francois; Vandeputte, Marc; Houston, Ross D.. |
| Background: European sea bass (Dicentrarchus labrax) is one of the most important species for European aquaculture. Viral nervous necrosis (VNN), commonly caused by the redspotted grouper nervous necrosis virus (RGNNV), can result in high levels of morbidity and mortality, mainly during the larval and juvenile stages of cultured sea bass. In the absence of efficient therapeutic treatments, selective breeding for host resistance offers a promising strategy to control this disease. Our study aimed at investigating genetic resistance to VNN and genomic-based approaches to improve disease resistance by selective breeding. A population of 1538 sea bass juveniles from a factorial cross between 48 sires and 17 dams was challenged with RGNNV with mortalities and... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00445/55676/57357.pdf |
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| Registros recuperados: 41 | |
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