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| Registros recuperados: 13 | |
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| Soufi-kechaou, Emna; Jaouen, Pascal; Ben Amar, Raja; Berge, Jean-pascal. |
| Enzymatic hydrolysis of cuttlefish viscera by Protamex, Pepsin, Delvolase and Multifect Neutral was investigated in a batch reactor during 6 hours with an hourly sampling. The influence of the enzyme used and the hydrolysis conditions (operating pH, temperature) was studied with regards to the extent of proteolytic degradation and to molecular weight distribution of the peptides. After 360 min of hydrolysis, cuttlefish viscera treated with Pepsin yielded significantly (p < 0.05) higher protein recoveries compared to the substrate autolysed or treated with other external proteases. However, the amino acid composition showed that this was Protamex hydrolysis which gave the highest percentage of essential amino acids. At least, according to the FPLC... |
| Tipo: Text |
Palavras-chave: Enzymatic hydrolysis; Cuttlefish Protein Hydrolysates (CPH); Cuttlefish By-Products (CBP); Essential Amino Acids (EAA); Protein recovery. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00114/22508/20191.pdf |
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| Picot, Laurent; Ravallec, Rozenn; Fouchereau-peron, Martine; Vandanjon, Laurent; Jaouen, Pascal; Chaplain-derouiniot, Maryse; Guerard, Fabienne; Chabeaud, Aurelie; Legal, Yves; Martinez Alvarez, Oscar; Berge, Jean-pascal; Piot, Jean-marie; Batista, Irineu; Pires, Carla; Thorkelsson, Gudjon; Delannoy, Charles; Jakobsen, Greta; Johansson, Inger; Bourseau, Patrick. |
| BACKGROUND: Numerous studies have demonstrated that in vitro controlled enzymatic hydrolysis of fish and shellfish proteins leads to bioactive peptides. Ultrafiltration (UF) and/or nanofiltration (NF) can be used to refine hydrolysates and also to fractionate them in order to obtain a peptide population enriched in selected sizes. This study was designed to highlight the impact of controlled UF and NF on the stability of biological activities of an industrial fish protein hydrolysate (FPH) and to understand whether fractionation could improve its content in bioactive peptides. RESULTS: The starting fish protein hydrolysate exhibited a balanced amino acid composition, a reproducible molecular weight (MW) profile, and a low sodium chloride content, allowing... |
| Tipo: Text |
Palavras-chave: Fish protein hydrolysate; Ultrafiltration; Nanofiltration; Membrane separation; Fractionation process; Bioactive peptide. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00011/12217/9259.pdf |
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| Walha, Khaled; Ben Amar, Raja; Masse, Anthony; Bourseau, Patrick; Cardinal, Mireille; Cornet, Josiane; Prost, Carole; Jaouen, Pascal. |
| Tuna cooking juices contain high organic load preventing the rejection in the environment without treatment. But the effluents present an interesting fishy odour and it is worth recovering aroma compounds. In this work, two industrial tuna cooking juices were concentrated by nanofiltration. Nanofiltration performance was discussed in terms of permeation fluxes, organic matter retention and impact on the aromatic properties of juices. NF sharply decreases the global intensity of juices and modifies their aromatic equilibrium. However, the main characteristics and the marine nature of juices were kept. A pre-treatment by microfiltration (MF) induces a marked increase in permeation fluxes during NF concentration while it slightly affects the aromatic... |
| Tipo: Text |
Palavras-chave: Tuna cooking juice; Aromas concentration; Membrane separation; Nanofiltration; Sensory analysis. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00016/12711/9689.pdf |
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| Kechaou, Emna; Durnay, Justine; Donnay-moreno, Claire; Jaouen, Pascal; Gouygou, Jean-paul; Berge, Jean-pascal; Ben Arnar, Raja. |
| Total lipid and phospholipid recovery as well as amino acid quality and composition from cuttlefish (Sepia officinalis) and sardine (Sardina pilchardus) were compared. Enzymatic hydrolyses were performed using the three proteases Protamex, Alcalase, and Flavourzyme by the pH-stat method (24 h, pH 8, 50 degrees C). Three fractions were generated: an insoluble sludge, a soluble aqueous phase, and an oily phase. For each fraction, lipids, phospholipids, and proteins were quantified. Quantitative and qualitative analyses of the raw material and hydrolysates were performed. The degree of hydrolysis (DH) for cuttlefish viscera was 3.2% using Protamex, 6.8% using Flavourzyme, and 7% using Alcalase. DH for sardine viscera was 1.9% (using Flavourzyme), 3.1% (using... |
| Tipo: Text |
Palavras-chave: Sardine; Viscera; Proteolysis; Marine lipids; Cuttlefish; By products. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6366.pdf |
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| Thorkelsson, G; Sigurgisladottir, S; Geirsdottir, M; Jóhannsson, R; Guerard, Fabienne; Chabeaud, Aurelie; Bourseau, Patrick; Vandanjon, Laurent; Jaouen, Pascal; Chaplain-derouiniot, M; Fouchereau-peron, Martine; Martinez Alvarez, Oscar; Le Gal, Yves; Ravallec-ple, Rozenn; Picot, Laurent; Berge, Jean-pascal; Delannoy, Charles; Jakobsen, Greta; Johansson, I; Batista, Ireneu. |
| Tipo: Text |
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| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/00056/16747/14184.pdf |
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| Boyen, Catherine; Jaouen, Pascal; Blanchard, Gilbert; Compere, Chantal; Dufour, Alain; Durand, Patrick; Guerard, Fabienne; Hallouin, Florence; Jebbar, Mohamed; Le Blay, Gwenaelle; Le Deit, Hervé; Le Seyec, Jocelyne; Monks, Brian; Portal-sellin, Rachel; Probert, Ian; Pruvost, Jeremy. |
| Marine (= blue) biotechnology, i.e. the utilization of marine bio-resources as a target or source of biotechnological applications, is a field with massive potential for innovation and economic growth. In a context of rapid climate change and increasing pressure on natural resources, renewed interest in marine biotechnology has been promoted by application of recent methodological and technological advances, notably in bioprocessing and in the various –omics domains, to the study of marine biodiversity. Marine biological resources potentially represent a sustainable raw material for exploitation in diverse fields, including nutrition, health, agriculture, aquaculture, energy, environment, and cosmetics. Marine biotechnology is now recognized as a... |
| Tipo: Text |
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| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00273/38460/36860.pdf |
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| Bourseau, Patrick; Vandanjon, Laurent; Jaouen, Pascal; Chaplain-derouiniot, Maryse; Masse, Anthony; Guerard, Fabienne; Chabeaud, Aurélie; Fouchereau-peron, Martine; Le Gal, Yves; Ravallec-ple, Rosenn; Berge, Jean-pascal; Picot, Laurent; Piot, Jean -marie; Batista, Ireneu; Thorkelsson, Gudjon; Delannoy, Charles; Jakobsen, Greta; Johansson, Inger. |
| The production by enzymatic treatment of fish protein hydrolysates (FPH) is a promising route to add value to fisheries proteinic co-products (fish frames, heads etc.). Indeed, FPH possess good nutritional properties and biological activities for food and feed uses. Pressure-driven membrane separations such as ultrafiltration (UF) and nanofiltration (NF) can be used after the hydrolysis to, increase the specific activities of the FPH. This paper discusses the impact of a two-step UF/NF process producing four different fractions on two industrial FPH with different hydrolysis degrees. Fractionation is carried out in "realistic" conditions for an industrial process, on highly concentrated FPH solutions (about 100 g of dry matter/L) at a high volume reduction... |
| Tipo: Text |
Palavras-chave: Peptidic profile; Fractionation process; Membrane separation; Nanofiltration; Ultrafiltration; FPH; Fish protein hydrolysate. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6653.pdf |
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| Kechaou, Emna; Berge, Jean-pascal; Jaouen, Pascal; Ben Amar, Raja. |
| Protein hydrolysate was prepared from common cuttlefish Sepia officinalis by Pepsin. Hydrolysis conditions (time, temperature, and enzyme activity) were optimized by response surface methodology (RSM) using a factorial design. The regression coefficient was close to 0.999, observed during both experimental and validation runs, and indicated the validity of prediction model. An enzyme activity to substrate ratio (X1), 0.04 AU/g protein; time (X2), 85 minutes; and temperature (X3), 45 °C were found to be the optimum conditions for a higher degree of hydrolysis (21%) and nitrogen recovery (90%) using Pepsin. According to amino acid analysis results and chemical score, methionine and histidine are the limiting amino acids in the hydrolysates, in that order.... |
| Tipo: Text |
Palavras-chave: Cuttlefish protein hydrolysate (CPH); Amino acid; Enzyme; Sepia officinalis. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00182/29285/27715.pdf |
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| Bourseau, Patrick; Chabeaud, Aurélie; Vandanjon, Laurent; Masse, Anthony; Jaouen, Pascal; Fleurence, Joël; Berge, Jean-pascal. |
| This review reports some interesting works on the recovery and fractionation by membrane techniques of marine compounds resulting from hydrolysis of fish by-products such as proteins or peptides, lipids or fatty acids, and carbohydrates. Ultrafiltration (UF) appears as the most widely used technique for such applications. It must be noted that only a few works deal with the optimization or the enhancement of processes for the recovery of added value molecules. Indeed, most of works use UF as a convenient and simple tool for solute fractionation, for this reason the influence of operating parameters on membrane separation efficiency is unfortunately seldom considered. When attention is paid in a study to the process development, it is specified in this... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-7408.pdf |
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| Registros recuperados: 13 | |
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