SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Cardoso,Milena Barcelos; Kaltchuk-Santos,Eliane; Mundstock,Elsa Cristina de; Bodanese-Zanettini,Maria Helena.

Anthers obtained from flowers buds of soybean cultivar IAS-5 were cultured in two basal culture media (B5 and B5 long). Cytological examinations of the in vitro anthers were performed during the first 20 days of culture to assay the viability (by propionic-carmine and fluorescein diacetate tests) and the stage of development of pollen grains. The frequencies of viable pollen grains varied significantly between bud sizes on the propionic-carmine analysis. The basal culture media and bud size had no clear effect on the frequencies of binucleate symmetrical and multinucleate pollen grains. Chromosome counts of metaphasic microspores throughout the culture period showed microspores with higher ploidy level in addition to normal chromosome number (n=20).

Effect of temperature shock on soybean microspore embryogenesis

Palavras-chave: Glycine max; FDA; Binucleate pollen grains; Multinucleate pollen grains; Spontaneous diploidization.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500005

Moraes,Ana Paula de; Bonadese-Zanettini,Maria Helena; Callegari-Jacques,Sídia Maria; Kaltchuk-Santos,Eliane.

In this study the effect of cold and heat shock on androgenetic induction in soybean anther culture was tested. Anthers of soybean were subjected to 4 and 33ºC, while the anthers used for control were maintained at 25ºC. Cytological analysis done during the 30 days of culture showed that frequencies of symmetrical binucleated and multicellular pollen grain did not differ among temperature treatments. Multicellular pollen grain could be formed by symmetrical division, as well as by assymetrical division. In relation to the embryo induction, the results of the treatments did not differ too. These results suggested that these treatments did not induce a sporophytic pathway in soybean.

Morphological markers for microspore developmental stage in maize

Palavras-chave: Anther culture; Soybean; Androgenesis; Symmetrical mitosis; Temperature shock; Stress.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400006

Moraes,Ana Paula de; Bered,Fernanda; Carvalho,Fernando Irajá Felix de; Kaltchuk-Santos,Eliane.

The use of maize in anther culture has been limited because only few genotypes presented a high androgenetic potential. Obtaining the proper stage of microspore development at culture initiation is one of the most crucial factors for success in the androgenesis. For Brazilian maize genotypes there are no studies reporting a correlation between cytological features and morphological parameters. In this study, morphological parameters were recorded and associated with cytological specific stages of the the microsporogenesis in two Brazilian maize genotypes that were sowed in different places (field and growing chamber). For both genotypes, the plants of the growing chamber presented a delay in development. Spikelets length and anther length/spikelet length...

Evaluation of gelling agents on anther culture: response of two soybean cultivars

Palavras-chave: Androgenesis; Anther culture; Microsporogenesis; Zea mays.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500006

Cardoso,Milena Barcelos; Bodanese-Zanettini,Maria Helena; Mundstock,Elsa Cristina de; Kaltchuk-Santos,Eliane.

Anthers of two soybean cultivars were cultured in B5 long basal culture media gelled with agarose or PhytagelTM. Cytological examinations of the anthers were carried out during the first 45 days of culture to assay the viability and developmental stage of microspores. Frequency of callus formation was recorded at 45 days of culture. The analysis of variance of the microspore viability assay showed significant Cultivar X Gelling Agent X Day of Culture interactions. The frequencies of viable microspores decreased significantly with time of culture, within each cultivar and gelling agent tested. The interaction Day X Cultivar was significant for the frequencies of binucleate symmetrical grains and multinucleate/multicellular structures. The effect of gelling...

Genetic and ecological niche modeling of Calydorea crocoides (Iridaceae): an endemic species of Subtropical Highland Grasslands

Palavras-chave: Anther culture; Glycine max; Culture media; Binucleate pollen grains; Multinucleate/multicellular structures; Callus formation.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700004

de Alencar,Juliana Lustosa Matos; Kaltchuk-Santos,Eliane; Fachinetto,Juliana; Tacuatiá,Luana Olinda; Forni-Martins,Eliana Regina; Stiehl-Alves,Eudes Maria; de Souza-Chies,Tatiana Teixeira.

Abstract Evolutionary analyses have been widely used for evaluation of genetic diversity of natural populations and correlate these data to the fitness of the species, especially in the case of threatened species. Calydorea crocoides occurs in a restricted area at altitudes from 800 to 1500 m in southern Brazil and is considered endangered. A study assessing genetic diversity, cytogenetic features and ecological niche was performed aiming to characterize C. crocoides by multidisciplinary approaches. Molecular data highlighted that most of the total variation (76%; p < 0.001) was found within populations and the parameters of genetic diversity were high at the species level (PPB = 98.97%; I = 0.4319; h = 0.2821). Gene flow (Nm) was estimated in 0.97...

Cytological and genome size data analyzed in a phylogenetic frame: Evolutionary implications concerning Sisyrinchium taxa (Iridaceae: Iridoideae)

Palavras-chave: Genetic differentiation; ISSR markers; Pollen viability; Cytogenetic analyses; Restricted distributed species.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200327

Burchardt,Paula; Souza-Chies,Tatiana T.; Chauveau,Olivier; Callegari-Jacques,Sidia M.; Brisolara-Corrêa,Lauís; Inácio,Camila Dellanhese; Eggers,Lilian; Siljak-Yakovlev,Sonja; de Campos,José Marcello Salabert; Kaltchuk-Santos,Eliane.

Abstract Sisyrinchium is the largest genus of Iridaceae in the Americas and has the greatest amount of cytological data available. This study aimed at investigating how genomes evolved in this genus. Chromosome number, genome size and altitude from species of sect. Viperella were analyzed in a phylogenetic context. Meiotic and pollen analyses were performed to assess reproductive success of natural populations, especially from those polyploid taxa. Character optimizations revealed that the common ancestor of sect. Viperella was probably diploid (2n = 2x =18) with two subsequent polyplodization events. Total DNA content (2C) varied considerably across the phylogeny with larger genomes detected mainly in polyploid species. Altitude also varied across the...

Genetics, evolution and conservation of Bromeliaceae

Palavras-chave: Ancestral chromosome number; DNA content; Meiotic behavior; Pollen viability; Polyploidy.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200288

Zanella,Camila M.; Janke,Aline; Palma-Silva,Clarisse; Kaltchuk-Santos,Eliane; Pinheiro,Felipe G.; Paggi,Gecele M.; Soares,Luis E.S.; Goetze,Márcia; Büttow,Miriam V.; Bered,Fernanda.

Bromeliaceae is a morphologically distinctive and ecologically diverse family originating in the New World. Three centers of diversity, 58 genera, and about 3,140 bromeliad species are currently recognized. We compiled all of the studies related to the reproductive biology, genetic diversity, and population structure of the Bromeliaceae, and discuss the evolution and conservation of this family. Bromeliads are preferentially pollinated by vertebrates and show marked variation in breeding systems, from predominant inbreeding to predominant outcrossing, as well as constancy in chromosome number (2n = 2x = 50). Autogamous or mixed mating system bromeliads have a high inbreeding coefficient (F IS), while outcrossing species show low F IS. The degree of...

Population genetic structure of Sisyrinchium micranthum Cav. (Iridaceae) in Itapuã State Park, Southern Brazil

Palavras-chave: Bromeliads; Cytogenetics; Genetic diversity; Population structure; Reproductive biology.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600017

Tacuatiá,Luana Olinda; Eggers,Lilian; Kaltchuk-Santos,Eliane; Souza-Chies,Tatiana T..

Sisyrinchium micranthum Cav. is a member of the family Iridaceae, which is distributed over the American continent. In Brazil, this species is found, not only in disturbed areas and coastal regions, but is also very common in urban centers, such as public parks, during the spring. Chromosome counts for North American specimens are 2n = 32 and 2n = 48, whereas in southern Brazil, there is a polyploidy series with three chromosome numbers, 2n = 16, 2n = 32, and 2n = 48. Population analyses using DNA molecular markers are inexistent for this species, in spite of its wide distribution and morphological variation. To study the genetic population structure of S. micranthum, five natural populations were accessed in a conservation park within the Atlantic Rain...

Agronomic performance, chromosomal stability and resistance to velvetbean caterpillar of transgenic soybean expressing cry1Ac gene

Palavras-chave: Iridaceae; ISSR-PCR; Mating system; Population genetics; Sisyrinchium micranthum.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100014

PAB

Homrich,Milena Schenkel; Passaglia,Luciane Maria Pereira; Pereira,Jorge Fernando; Bertagnolli,Paulo Fernando; Salvadori,José Roberto; Nicolau,Marcio; Kaltchuk-Santos,Eliane; Alves,Laura Bianchessi; Bodanese-Zanettini,Maria Helena.

The objective of this work was to analyze the agronomic performance and chromosomal stability of transgenic homozygous progenies of soybean [Glycine max (L.) Merrill.], and to confirm the resistance of these plants against Anticarsia gemmatalis. Eleven progenies expressing cry1Ac, hpt and gusA genes were evaluated for agronomic characteristics in relation to the nontransformed parent IAS 5 cultivar. Cytogenetical analysis was carried out on transgenic and nontransgenic plants. Two out of the 11 transgenic progenies were also evaluated, in vitro and in vivo, for resistance to A. gemmatalis. Two negative controls were used in resistance bioassays: a transgenic homozygous line, containing only the gusA reporter gene, and nontransgenic 'IAS 5' plants. The...