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| Registros recuperados: 48 | |
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| Zakhour, Maha; Maalouf, Haifa; Di Bartolo, Ilaria; Haugarreau, Larissa; Le Guyader, Francoise; Ruvoen-clouet, Nathalie; Le Saux, Jean-claude; Ruggeri, Franco Maria; Pommepuy, Monique; Le Pendu, Jacques. |
| Noroviruses (NoV) are major agents of acute gastroenteritis in humans and the primary pathogens of shellfish-related outbreaks. Previous studies showed that some human strains bind to oyster tissues through carbohydrate ligands that are similar to their human receptors. Thus, based on presentation of shared norovirus carbohydrate ligands, oysters could selectively concentrate animal strains with increased ability to overcome species barriers. In comparison with human GI and GII strains, bovine GIII NoV strains, although frequently detected in bovine feces and waters of two estuaries of Brittany, were seldom detected in oysters grown in these estuaries. Characterization of the carbohydrate ligand from a new GIII strain indicated recognition of the... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00014/12534/9527.pdf |
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| Schaeffer, Julien; Treguier, Cathy; Piquet, Jean-come; Gachelin, Sonia; Cochennec-laureau, Nathalie; Le Saux, Jean-claude; Garry, Pascal; Le Guyader, Soizick. |
| As human population increases worldwide, water quality will become increasingly problematic, and food consumed raw may be of higher risk. This is already evident for oysters grown in coastal areas - despite regulations based on bacterial indicators, oysters are still implicated in food-borne outbreaks worldwide. The pathogens most frequently detected are human noroviruses, which are shed at high concentrations in human excreta and are very resistant to environmental conditions. Sewage treatment plants usually apply a variety of steps such as activated sludge treatment, chlorine or UV disinfection to eliminate contaminants, these processes have variable efficacy. This study demonstrates the impact of replacing an old lagoon-based sewage treatment plant with... |
| Tipo: Text |
Palavras-chave: Norovirus; Shellfish safety; Sewage treatment; Coastal water quality. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00450/56140/57707.pdf |
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| Parnaudeau, Sylvain; Le Mennec, Cecile; Miura, Takayuki; Le Saux, Jean-claude; Le Guyader, Soizick. |
| Human enteric viruses discharged in raw sewage are hightly resistant and may be detected in surface waters, coastal areas and shellfish. Whereas noroviruses, responsible for relatively benign acute gastroentritis, are the most common agents implicated in shellfish borne outbreaks, other human viruses which cause severe human diseases, such as hepatitis A virus, may also be transmitted. Environmental studies have demonstrated that virus concentrations detected in shellfish are higher than in surrounding waters. This concentration may explain the high prevalence of noroviruses detected in various shellfish species collected in different countries. If many questions remain regarding viral contamination of shellfish, the most important seems to be the... |
| Tipo: Text |
Palavras-chave: Shellfish; Human enteric viruses; Norovirus; Hepatitis A virus; Coquillage; Virus entérique humain; Norovirus; Virus de l'hépatite A. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00317/42849/42258.pdf |
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| Garry, Pascal; Le Saux, Jean-claude; Hervio Heath, Dominique; Le Guyader, Soizick. |
| La Microbiologie sanitaire, thème de recherche fédérant notre laboratoire, nous a permis d’obtenir des résultats intéressants qui sont présentés dans ce rapport et ont été valorisés par des publications ou la participation des membres de l’équipe à des congrès scientifiques. Cette thématique nous permet surtout des collaborations variées au travers des projets de recherche portés par l’équipe. L’élargissement de nos activités en intégrant la coordination REMI constitue un point important à souligner complétant nos actions de référence, d’expertise et de recherche. L’attrait et l’intérêt scientifique suscité par la microbiologie sanitaire a été démontré par le succès rencontré par le colloque organisé à Brest en lien avec la fin du projet européen... |
| Tipo: Text |
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| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00380/49138/49574.pdf |
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| Le Guyader, Soizick; Krol, Joanna; Ambert-balay, Katia; Ruvoen-clouet, Nathalie; Desaubliaux, Benedicte; Parnaudeau, Sylvain; Le Saux, Jean-claude; Ponge, Agnes; Pothier, Pierre; Atmar, Robert L.; Le Pendu, Jacques. |
| Noroviruses have been recognized to be the predominant agents of nonbacterial gastroenteritis outbreaks in humans, and their transmission via contaminated shellfish consumption has been demonstrated. Norovirus laboratory experiments, volunteer challenge studies, and community gastroenteritis outbreak investigations have identified human genetic susceptibility factors related to histo-blood group antigen expression. Following a banquet in Brittany, France, in February 2008, gastroenteritis cases were linked to oyster consumption. This study identified an association of the norovirus illnesses with histo-blood group expression, and oyster contamination with norovirus was confirmed by qualitative and quantitative analyses. The secretor phenotype was... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00002/11309/8050.pdf |
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| Le Saux, Jean-claude; Serais, Ophelie; Krol, Joanna; Parnaudeau, Sylvain; Salvagnac, P.; Delmas, G.; Cicchelero, V.; Claudet, J.; Pothier, P.; Balay, K.; Fiandrino, Annie; Pommepuy, Monique; Le Guyader, Soizick. |
| Infectious diseases linked to the consumption of raw shellfish have long been identified. Over the past century, various strategies have been set up in shellfish growing areas throughout the world to guarantee the sanitary quality of shellfish and to protect consumers. However despite sanitary improvements, human enteric viruses - especially Hepatitis A virus and norovirus– have been found to be associated with shellfish outbreaks. A recent example demonstrated the impact of storm events. Following heavy rain and sewage overflow, shellfish beds were contaminated and the shellfish from them were marketed after depuration. However, since viruses persist longer than fecal contamination indicator bacteria, several clusters of gastroenteritis cases were... |
| Tipo: Text |
Palavras-chave: Virus; Shellfish; Contamination events. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00066/17736/15257.pdf |
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| Monfort, Patrick; Piclet, Guy; Le Naour, Gwenael; Le Saux, Jean-claude; Raguenes, Pierre; Boulben, Sylviane; Le Gal, Dominique; Minet, Jacques; Venien, Jacqueline; Cormier, Michel; Plusquellec, Alain. |
| Cette étude a porté sur la recherche des bactéries appartenant au genre Listeria (Listeria spp.) dans 61 échantillons de coquillages issus de quelques zones de production situées dans le département du Finistère. Ces investigations, effectuées au cours de deux périodes (janvier-février et juillet -août 1994), ont mis en évidence la présence régulière des Listeria dans les bivalves (L. spp.= 49.2% ), la part non négligeable de Listeria monocytogenes (L.m. = 11.5%), la multiplicité d'espèces sur quelques échantillons testés (Baie de Morlaix) ainsi qu'une large distribution de ce genre bactérien. Par ailleurs, il est démontré une incidence saisonnière,la période hivernale se révélant la plus critique. Cette information, intéressante sur le plan sanitaire,... |
| Tipo: Text |
Palavras-chave: Listeria; Bactéries pathogènes; Coquillages; Produits marins; Estuaires; Listeria; Pathogenic bacteria; Shellfishes; Seafood products; Estuaries. |
| Ano: 1995 |
URL: http://archimer.ifremer.fr/doc/00104/21555/19134.pdf |
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| Treguier, Cathy; Schaeffer, Julien; Le Saux, Jean-claude; Cochennec-laureau, Nathalie; Le Guyader, Soizick. |
| Les norovirus (NoV) sont responsables des épidémies hivernales de gastroentérites aiguës dans la population. Ils sont excrétés dans les fèces humains, puis se retrouvent, après épuration insuffisante, dans le milieu marin naturel où ils peuvent contaminer des coquillages, qui à leur tour risquent de déclencher des gastroentérites chez les consommateurs. Cette étude vise à améliorer les connaissances sur la contamination des coquillages en élevage en rivière d’Auray par les NoV, en lien avec les bactéries témoins de contamination fécale Escherichia coli (E. coli). L’objectif est de suivre les flux de NoV et d’E. coli apportés par différents rejets (dont une station d’épuration) impactant la zone conchylicole et leur influence sur la qualité des coquillages... |
| Tipo: Text |
Palavras-chave: Norovirus; Coquillages; Contamination; Station d’épuration; Flux; Rivière d’Auray; Escherichia coli.. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00267/37778/35829.pdf |
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| Riou, Philippe; Le Saux, Jean-claude; Dumas, Franck; Le Guyader, Soizick; Le Goff, R.; Maheux, Frank; Lamort, Laure; Pommepuy, Monique. |
| Climate change contributes to local weather modifications such as storm events and heavy rainfall, and in particular, changes in the frequency and intensity of extreme storm and rainfall events. Observations have indicated that rainfall-runoff processes, like sewage overflow may induce fecal contamination in coastal water. Pathogens present in runoff water could therefore contaminate shellfish and be responsible for food-borne disease outbreaks. Mathematical models for this were used to predict the effect of climate modifications on shellfish quality. Using a hydrodynamic model, simulations of water quality conditions under a variety of storm event scenarios were run and are presented here for a harvesting area in France. Major storm events occurring in... |
| Tipo: Text |
Palavras-chave: Modeling; Seawater fecal contamination; E. coli; Virus; Shellfish; Climate change. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/00066/17739/15261.pdf |
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| Guillois-becel, Y.; Couturier, E.; Le Saux, Jean-claude; Roque-afonso, A. M.; Le Guyader, Soizick; Le Goas, A.; Pernes, J.; Le Bechec, S.; Briand, A.; Robert, C.; Dussaix, E.; Pommepuy, Monique; Vaillant, V.. |
| Following the notification of nine hepatitis A cases clustered in the Cotes d'Armor district in northwestern France, epidemiological, environmental and microbiological investigations were set up in order to identify the source and vehicle of contamination and implement control measures. In total, 111 cases were identified in the outbreak, all of whom lived or had stayed as tourists in the Cotes d'Armor district. Of the cases, 87% had eaten raw shellfish, and 81% specifically oysters. Traceback investigations carried out on raw shellfish consumed by the cases showed that the raw shellfish originated from a single shellfish farm. The shellfish were probably contaminated either in the submersible tanks or in a depuration land-based tank where they were... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6685.pdf |
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| Mieszkin, Sophie; Furet, Jean-pierre; Corthier, Gerard; Pommepuy, Monique; Le Saux, Jean-claude; Bougeard, Morgane; Hervio Heath, Dominique; Gourmelon, Michele. |
| The microbiological quality of coastal waters and shellfish harvesting areas in Brittany (France) can be affected by faecal pollutions from human activities and animal breeding (especially pigs and cattle). To discriminate among faecal pollution of human and animal origin, a library-independent microbial source tracking method was selected: Bacteroidales host-specific 16S rRNA gene markers by real-time Polymerase Chain Reaction (PCR). A human-specific Bacteroidales marker (Hum-1-Bac) was designed. Tested on faecal samples, the Hum-1-Bac marker showed 95 % sensitivity and 95 % specificity (n= 80). Average values (± STD) of the Hum-1-Bac marker were found to be 7.3 ± 1.4 16S rRNA gene copies per g wet faeces in human faeces samples (n=10) and 5.7 ± 1.3 log10... |
| Tipo: Text |
Palavras-chave: Microbial Source Tracking; Host-Specific Bacteroidales Marker; 16S rRNA Gene; Real-Time PCR; Faecal Contamination. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00021/13259/10306.pdf |
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| Pommepuy, Monique; Hervio Heath, Dominique; Caprais, Marie-paule; Gourmelon, Michele; Le Saux, Jean-claude; Le Guyader, Soizick. |
| Introduction : "The occurrence of pathogenic microorganisms in seawater or in shellfish could exist anytime sewage from human or animal origin would be discharged to the coast" (Metcalf, 1982). According to the diseases occurring in the human population or in animals, pathogens might be present in recreational waters or in shellfish. Thus, the presence of human enteric viruses (norovirus, astrovirus, rotavirus, hepatitisAvirus (HAV)) and pathogenic bacteria (Salmonella, Listeria monocytogenes, Shiga-toxin-producing Escherichia coli (STEC), Vibrio cholerae, Vibrio parahaemolyticus, etc.) has been reported in coastal areas for a long time (Colwell, 1978; Metcalf, 1978; Melnick et al., 1979; Grimes, 1991; Bosch et al., 2001;Kong et al., 2002). These... |
| Tipo: Text |
Palavras-chave: Estimating model; Infection dose; Microorganisms; STEC; Bacteria; Virus; Fecal contamination; Pathogenic agent; Marine sediment; Estuarine water; Coastal area; Marine water. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1207.pdf |
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| Le Saux, Jean-claude; Derolez, Valerie; Brest, G.; Le Guyader, Soizick; Pommepuy, Monique. |
| For shellfish, the viral contamination clearly occurs in the first step of the process i.e. in growing and harvesting areas. As opposed to other foods, there is no proof that other routes (foodhandlers, aerosol), could be at the origin of the seafood contamination (Koopmans & Duizer, 2004). Most of the time, untreated sewage are at the origin of the presence of viruses in shellfish. However, direct contamination by ill people working in the growing area, has also been reported (Berg et al., 2000; Butt et al, 2004). |
| Tipo: Text |
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| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/00066/17733/15254.pdf |
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| Thomas, Gerard; Piquet, Jean-come; Rouyer, Pascal; Audat, Cécile; Le Saux, Jean-claude; Ryckaert, Mireille; Fillon, Alain. |
| Le rapport se propose de dresser le bilan actuel de l’état sanitaire microbiologique des zones d’élevage ou de pêche professionnelle de coquillages dans les pertuis charentais. Bien qu’il soit principalement axé sur le volet microbiologique, les questions relatives aux teneurs des coquillages concernant trois métaux lourds (plomb, mercure, cadmium) sont également abordées, car le classement sanitaire des zones conchylicoles dépend aussi de ces paramètres. Les résultats du REMI* montrent que sur les dix dernières années, la situation des zones classées est restée globalement stable ou en légère amélioration, ce qui est à mettre au crédit des progrès en terme d’assainissement. Néanmoins, diverses études sanitaires révèlent la persistance d’un certain nombre... |
| Tipo: Text |
Palavras-chave: Zones conchylicoles; Etat sanitaire; Microbiologie; Métaux lourds. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00123/23420/21247.pdf |
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| Registros recuperados: 48 | |
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