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Shibao,Priscila Y T; Anjolette,Fernando A P; Lopes,Norberto P.; Arantes,Eliane C.. |
Background Toad secretions are a source of molecules with potential biotechnological application on a wide spectrum of diseases. Toads from theRhinella family have two kinds of poisonous glands, namely granular and mucous glands. Rhinella schneideritoads produce granular secretions that comprise a great number of molecules, including serine proteases inhibitors. Serine proteases, such as trypsin, chymotrypsin and elastase, are enzymes that have a serine amino acid into its catalytic site and can be found in a large number of vertebrate species and pathogenic microorganisms. Therefore, the present work aims to purify a serine protease inhibitor from Rhinella schneiderigranular secretions.Findings This study presents the protocol used to purify a serine... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bufadienolide; Rhinella schneideri; Serine protease inhibitor. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100501 |
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Souza,Jeana M.E.; Chang,Marilene R.; Brito,Daniela Z.; Farias,Katyuce S.; Damasceno-Junior,Geraldo A.; Turatti,Izabel C.C.; Lopes,Norberto P.; Santos,Edson A.; Carollo,Carlos A.. |
Abstract We evaluated the antimicrobial activity of Aspilia latissima - an abundant plant from the Brazilian Pantanal region - against Candida albicans, Candida parapsilosis, Candida krusei, Candida tropicalis, Pseudomonas aeruginosa, Enterococcus faecalis, Escherichia coli and Staphylococcus aureus. The crude extracts and fractions showed activity in all tested microorganisms. The chloroform fraction of the leaves and roots showed the most antimicrobial activity against S. aureus, with an MIC of 500 μg/mL. This fraction was submitted to bioautographic assays to characterize the activity of the compounds. Two bands from the leaves (L-A and L-B) and three bands from the roots (R-C, R-D and R-E) were bioactive. Within the root-derived bands, the terpene... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspilia latissima; Staphylococcus aureus; Bioautographic; MIC; Terpenes. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401103 |
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