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| Registros recuperados: 32 | |
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| Kuboki, Noritaka; Yokoyama, Naoaki; Namangala, B; Okamura, Masashi; Inoue, Noboru; Takagi, Hideaki; Nakayama, Tomoko; Nishikawa, Yoshifumi; Ikehara, Yuzuru; Kojima, Naoya; 横山, 直明; 井上, 昇; 西川, 義文. |
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Palavras-chave: Adjuvant; African trypanosome; Immunization; Oligomannose-coated liposome(OML). |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2222 |
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| Zhang, Houshuang; Compaore, Muller K.A.; Lee, Eung-goo; Liao, Min; Zhang, Guohong; Sugimoto, Chihiro; Fujisaki, Kozo; Nishikawa, Yoshifumi; Xuan, Xuenan. |
| The cross-reactive antigens of Neospora caninum and Toxoplasma gondii are important in the exploration to determine the common mechanisms of parasite-host interaction. In this study, a gene encoding N. caninum apical membrane antigen 1 (NcAMA1) was identified by immunoscreening of a N. caninum tachyzoite cDNA expression library with antisera. from mice immunized with recombinant T gondii apical membrane antigen 1 (TgAMA 1). NcAMA1 was encoded by an open reading frame of 1695 bp, which encoded a protein of 564 amino acids. The single-copy NcAMA1 gene was interrupted by seven introns. NcAMA1 showed 73.6% amino acid identity to TgAMA1. Mouse polyclonal antibodies raised against the recombinant NcAMA1 (rNcAMA1) recognized a 69-kDa native parasite protein by... |
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Palavras-chave: Neospora caninum; Toxoplasma gondii; Apical membrane antigen 1; Cross-reactive; Invasion. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1034 |
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| Kawase, Osamu; Nishikawa, Yoshifumi; Bannai, Hiroshi; Matsuo, Tomohide; Xuan, Xuenan. |
| Toxoplasma gondii is an obligate intracellular protozoan parasite that invades a wide range of host cells. Upon encountering host cells, the parasite releases a large variety of proteins from secretory organelles, such as micronemes, rhoptries and dense granules. The secretion of microneme protein is essential for parasite invasion. We found that a secreted protein with an altered thrombospondin repeat of Toxoplasma gondii (TgSPATR) was a novel microneme protein, which was different from known microneme proteins that carry thrombospondin repeat domains. TgSPATR was secreted in response to an intra-parasitic elevation of Ca2+ and probably secreted during early stages of parasite invasion. Thus, we suggested that TgSPATR, new member of microneme secretory... |
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Palavras-chave: Ca2+-dependent secretion; Microneme protein; Secreted protein with an alteredthrombospondin repeat; Toxoplasma gondii. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2819 |
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| Leesombun, Arpron; Boonmasawai, Sookruetai; Nishikawa, Yoshifumi. |
| Neosporosis has a worldwide distribution and causes economic losses in farming, particularly by increasing the risk of abortion in cattle. This study investigated the effects of Thai piperaceae (Piper betle, P. nigrum, and P. sarmentosum) extracts on Neospora caninum infections in vitro and in vivo. In an in vitro parasite growth assay based on the green fluorescent protein (GFP) signal, P. betle was the most effective extract at inhibiting parasite growth in human foreskin fibroblast cells (IC50 of GFP-expressing N. caninum parasites, 22.1 mu g/ml). The P. betle extract, at 25 mu g per ml, inhibited parasite invasion into host cells. Furthermore, in two independent experiments, treating N. caninum-infected mice with the P. betle extract for 7 days... |
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Palavras-chave: Neospora caninum; Neosporosis; Piper betle; Piper nigrum; Piper sarmentosum; Ethanol extract. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4578 |
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| Zhou, Mo; Cao, Shinuo; Sevinc, Ferda; Sevinc, Mutlu; Ceylan, Onur; Liu, Mingming; Wang, Guanbo; Moumouni, Paul Franck Adjou; Jirapattharasate, Charoonluk; Suzuki, Hiroshi; Nishikawa, Yoshifumi; Xuan, Xuenan. |
| Considering the scarce information on occurrences of Toxoplasma gondii and Neospora caninum in domestic animals from Turkey, the aim of this study was to investigate the seroprevalence of these parasite infections in cattle, horses, sheep, goats and dogs in Turkey. The specific antibodies against T gondii and N. caninum were detected by iELISAs based on the recombinant TgSAG2 or NcSAG1 in a total of 2,039 serum samples from eleven provinces. The seroprevalence of T. gondii infections was 46.3%, 4.0%, 20.0%, 12.9% and 19.8%, that of N. caninum infections was 0.3%, 7.4%, 2.1%, 3.2% and 16.6% in the horses, cattle, sheep, goats and dogs, respectively. These results indicated that T gondii and N. caninum infections are prevalent in Turkish domestic animals. |
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Palavras-chave: ELISA; Neospora caninum; Toxoplasma gondii; Turkey. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4422 |
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| Ooka, Hideo; Terkawi, Mohamad A; Cao, Shinuo; Aboge, Gabriel; Goo, Youn-Kyoung; Luo, Yuzi; Li, Yan; Nishikawa, Yoshifumi; Igarashi, Ikuo; Xuan, Xuenan; 西川, 義文; 五十嵐, 郁男; 玄, 学南. |
| A cDNA encoding the Babesia microti 32-kDa protein was identified by serological immunoscreening of a cDNA expression library and designated as BmP32. The full length of BmP32 contains an open reading frame of 918 base pairs consisting of 306 amino acids having a significant homology with B. microti secreted antigen 1. Antiserum raised against recombinant protein (rBmP32) specifically reacted with a 32-kDa native protein of the parasite lysate using western blot analysis. The indirect immunofluorescent antibody test showed a preferable localization of BmP32 in the cytoplasm of the intra- and extracellular parasites. Moreover, BmP32 was secreted in the cytosol of infected erythrocytes, especially during the peak parasitemia and the recovery phase of the... |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3820 |
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| Sandagdorj, Narantsatsral; Goo, Youn-Kyoung; Terkawi, Mohamad Alaa; Soma, Takehisa; Luo, Yuzi; Li, Yan; Cao, Shinuo; Aboge, Gabriel Oluga; Nishikawa, Yoshifumi; Badgar, Battsetseg; Xuan, Xuenan. |
| Among the previously established enzyme-linked immunosorbent assays (ELISAs), an ELISA using the full length of recombinant thrombospondin-related adhesive protein of Babesia gibsoni (rBgTRAPf) is considered as the most sensitive diagnostic method for the detection of antibody to B. gibsoni in dogs. However, the expression of rBgTRAPf in high concentration is poor and thus limits its usefulness as diagnostic antigen. To improve its expression level, we have truncated BgTRAPf into two fragments having either N- or C-terminus (BgTRAPn or BgTRAPc). The expression of BgTRAPc protein in Escherichia coli yielded adequate recombinant protein. The specificity and sensitivity of ELISAs with the truncated proteins were determined using B. gibsoni-experimentally... |
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Palavras-chave: Babesia gibsoni; Diagnosis; Enzyme-linked immunosorbent assay (ELISA); Thrombospondin-related adhesive protein (TRAP). |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3115 |
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| Hara, Olgga A; Liao, Min; Baticados, Waren; Bannai, Hiroshi; Zhang, Guohong; Zhang, Sofa; Lee, Eung-goo; Nishikawa, Yoshifumi; Claveria, Florencia G.; Igarashi, Makoto; Nagasawa, Hideyuki; Xuan, Xuenan. |
| Neospora caninum infection is an important disease affecting bovine and canine populations worldwide. The dense granule antigen 7 of N. caninum (NcGRA7) is considered to be an immunodominant antigen. In the present study, the gene encoding truncated NcGRA7 (NcGRA7t) lacking an N-terminal signal peptide was expressed in Escherichia coli, and its diagnostic potential in an enzyme-linked immunosorbent assay (ELISA) was evaluated. The ELISA could clearly discriminate between known N. caninum-positive and -negative sera from dogs. Serum samples randomly collected from dogs in Japan and China were examined for the diagnosis of neosporosis using the ELISA. Twenty-three of 135 samples (17.03%) and 9 of 95 (9.47%) samples from Japan and China, respectively, were... |
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Palavras-chave: Canine; ELISA; Neospora caninum; NcGRA7. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/995 |
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| Xuan, Xuenan; Zhang, Sofa; Kamio, Tsugihiko; Tsushima, Y.; Kamada, Takenori; Nishikawa, Yoshifumi; Otsuka, Haruki; Karanis, Panagiotis; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Mikami, Takeshi; 玄, 学南; 西川, 義文; 五十嵐, 郁男. |
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Palavras-chave: C. parvum; P15; Vaccinia virus; Subunit vaccine. |
| Ano: 1999 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/314 |
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| Terkawi, M. A.; Aboge, G.; Jia, H.; Goo, Y-K.; Ooka, H.; Yamagishi, Junya; Nishikawa, Yoshifumi; Kawazu, Shin-ichiro; Fujisaki, K.; Xuan, Xuenan; 山岸, 潤也; 西川, 義文; 河津, 信一郎; 玄, 学南. |
| A novel gene encoding 27-kDa protein was identified by the screening of Babesia gibsoni cDNA library with acutely infected dog serum. The BgP27 is a single copy gene with a predicted open reading frame of 762 bp and 254 amino acids. The phylogenic analysis of the deduced amino acid of BgP27 demonstrated considerable identities with members of Plasmodium berghei circumsporozoite protein family that ranged between 18.4% and 22.8%. The BgP27 was expressed as a glutathione S-transferase fusion protein in Escherichia coli. The serum raised in mice against the recombinant protein specifically reacted with a 27-kDa protein in the extracts of B. gibsoni parasites. Confocal laser scanning microscopic observation showed high fluorescent reactivity with both... |
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Palavras-chave: Babesia gibsoni; Enzyme-linked immunosorbent assay; Deiagnostic performance. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2232 |
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| Nishikawa, Yoshifumi. |
| Neosporosis is caused by the intracellular protozoan parasite Neospora caninum. This major disease-causing pathogen is responsible for inducing abortion in cattle, and these adverse events occur sporadically all over the world, including Japan. Currently, there are no vaccines on the market against infection with N. caninum. Because live and attenuated vaccines against N. caninum have had safety and effectiveness issues, development of a next-generation vaccine is urgently required. To develop a vaccine against neosporosis, my laboratory has been focused on the following: 1) understanding the host immune responses against Neospora infection, 2) identifying vaccine antigens and 3) developing an effective antigen-delivery system. The research strategy taken... |
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Palavras-chave: Adjuvant; Cattle; Neospora caninum; Neosporosis; Vaccine. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4544 |
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| Bannai, Hiroshi; Nishikawa, Yoshifumi; Seo, Jin-yong; Nakamura, Chinatsu; Zhang, Sofa; Kimata, Isao; Takashima, Yasuhiro; Li, Junyou; Igarashi, Ikuo; Xuan, Xuenan; 西川, 義文; 五十嵐, 郁男; 玄, 学南. |
| An enzyme-linked immunosorbent assay (ELISA) based on the recombinant p23 of Cryptosporidium parvum was established for the detection of antibodies against C. parvum in cattle. The sensitivity and specificity of the ELISA were evaluated with the standard indirect fluorescent antibody test (IFAT) using sporozoites as antigens. Of 77 bovine sera collected from China, 20 (26.0%) were identified as positive by the IFAT. The same samples were tested with the ELISA. The optical densities at 415 nm were compared to the IFAT results and statistically analyzed to designate a provisional cut-off point. As a result, the cut-off point was concluded to be 0.08, which was considered to be the best condition in the light of its sensitivity (80%) and specificity (73.7%).... |
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Palavras-chave: Cryptosporidium parvum; P23; ELISA; IFAT. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/142 |
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| Zhou, Jinlin; Yang, Jun; Zhang, Guohong; Nishikawa, Yoshifumi; Fujisaki, Kozo; Xuan, Xuenan. |
| A cDNA encoding the apical membrane antigen-1 (AMA-1) homologue was obtained by immunoscreening a cDNA expression library prepared from Babesia gibsoni merozoite mRNA. The complete nucleotide sequence of the gene was 2062 bp. Computer analysis suggested that the sequence contains an open reading frame of 1794 bp with a coding capacity of approximately 66 kDa. Based on the homology analysis, this putative protein was designated as B. gibsoni AMA-1 (BgAMA-1). The BgAMA-1 gene was expressed in the Escherichia coli BL21 strain and used as the antigen in Western blotting and the enzyme-linked immunosorbent assay (ELISA). The results indicated that BgAMA-1 was recognized as an immunodominant antigen by the host immune system and that it induced a strong antibody... |
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Palavras-chave: Babesia gibsoni; AMA-1; Antibody response. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/815 |
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| Mahmoud, Motamed Elsayed; Fereig, Ragab; Nishikawa, Yoshifumi. |
| Toxoplasma gondii is a pathogen relevant to psychiatric disorders. We recently showed that reactivation of chronic T. gondii infection induced depression-like behaviors in mice. Furthermore, it has been hypothesized that depression-like behaviors are mediated via a host defense mechanism against invading pathogens; proximate mechanisms of this behavioral hypothesis remain unclear. In the present study, we investigate the contribution of indoleamine 2,3-dioxygenase (IDO), inflammation, and interferon gamma (IFN-gamma) to anhedonic and despair-related behaviors in T. gondii-infected mice by using sucrose preference and forced-swim tests, respectively. First, we confirmed that BALB/c mice exhibited both sickness and depression-like behaviors during acute... |
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Palavras-chave: Toxoplasma gondii; 1-methyl tryptophan; Minocycline; Interferon gamma; Sickness behavior; Anhedonic behavior; Despair-like behavior. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4507 |
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| Fereig, Ragab M.; Mohamed, Samy G. A.; Mahmoud, Hassan Y. A. H.; AbouLaila, Mahmoud Rezk; Guswanto, Azirwan; Thu-Thuy Nguyen; Mohamed, Adel Elsayed Ahmed; Inoue, Noboru; Igarashi, Ikuo; Nishikawa, Yoshifumi. |
| Babesia bovis, B. bigemina, Trypanosoma evansi, and Anaplasma marginate infections cause serious diseases in cattle, and are primarily transmitted by arthropod vectors (ticks for B. bovis, B. bigemina, and A. marginate and various types of flies for T. evansi). In the last few years, there have been many reports of a high prevalence of certain protozoan infections in northern Egypt, but no accurate or adequate data are available for the southern regions. Therefore, in this study, we screened for evidence of such diseases in economically important cattle species using serum samples. The seroprevalence of protozoan infections in cattle was determined with enzyme-linked immunosorbent assays using species-specific diagnostic antigens. In a total of 301 cattle... |
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Palavras-chave: Babesia bovis; Babesia bigemina; Trypanosoma evansi; Anaplasma marginale; Cattle; Egypt. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4579 |
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| Fereig, Ragab M.; Nishikawa, Yoshifumi. |
| Toxoplasmosis remains a life-threatening infection of humans and various domestic and wild animals worldwide. It is caused by the obligatory intracellular protozoan parasite Toxoplasma gondii. Peroxiredoxins (Prxs) are a family of antioxidant enzymes that protect cells from oxidative stress from hydroperoxides. In the recent years, several studies have reported the potential use of T. gondii-derived enzymes in triggering protective immunity against T. gondii infection. Therefore, this study was conducted to investigate the immunogenicity and protective efficacy of TgPrx3. In vitro stimulation of peritoneal macrophages with recombinant TgPrx3 protein fused to glutathione-S transferase (TgPrx3-GST) enhanced IL-12p40 production, indicating the... |
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Palavras-chave: Toxoplasma gondii; Peroxiredoxin 3; Vaccine; Immunization. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4569 |
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| Zhang, Houshuang; Nishikawa, Yoshifumi; Ikehara, Yuzuru; Kojima, Naoya; Yokoyama, Naoaki; Xuan, Xuenan. |
| Liposomes coated with neoglycolipids constructed with mannopentaose and dipalmitoylphosphatidylethanolamine (Man3-DPPE), referred to as M3-DPPE liposomes, have been shown to induce cellular immunity against antigens encapsulated therein. To evaluate whether these M3-DPPE liposomes have an adjuvant capacity against Neospora caninum infection, a novel immunization method utilizing soluble N. caninum apical membrane antigen 1 (NcAMA1) encapsulated in the M3-DPPE liposomes (M3-NcAMA1) was employed. The intent was to reduce offspring mortality from N. caninum infection in susceptible, pregnant BALB/c mice. The results revealed that BALB/c mice developed IgG antibodies specific to N. caninum. A significant amount of interferon (IFN)-γ production was... |
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Palavras-chave: Neospora caninum; Apical membrane antigen 1; Oligomannose-coated liposomes. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2821 |
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| Zhou, Mo; Cao, Shinuo; Luo, Yuzi; Liu, Mingming; Wang, Guanbo; Moumouni, Paul Franck Adjou; Jirapattharasate, Charoonluk; Iguchi, Aiko; Vudriko, Patrick; Terkawi, Mohamad Alaa; Lowenstein, Mario; Kern, Angela; Nishikawa, Yoshifumi; Suzuki, Hiroshi; Igarashi, Ikuo; Xuan, Xuenan. |
| Background: Babesia canis is an apicomplexan tick-transmitted hemoprotozoan responsible for causing canine babesiosis in Europe and west Asia. Despite its importance, there is no known rapid diagnostic kit detection of B. canis infection in dogs. The present study identified two novel antigens of B. canis and used the recombinant antigens to establish a rapid, specific and sensitive serodiagnostic technique for detection of B. canis infection. Methods: A complementary DNA (cDNA) expression library was constructed from the mRNA of B. canis and immunoscreened using B. canis-infected dog sera. The cDNAs encoding a merozoite surface antigen and a secreted antigen protein were identified and designated as BcMSA1 and BcSA1, respectively. The recombinant BcMSA1... |
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Palavras-chave: Babesia canis; Canine babesiosis; BcMSA1; BcSA1; ELISA; Immunochromatographic tests. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4420 |
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| Fereig, Ragab M.; AbouLaila, Mahmoud Rezk; Mohamed, Samy G. A.; Mahmoud, Hassan Y. A. H.; Ali, Alsagher O.; Ali, Asmaa F.; Hilali, Mosaad; Zaid, Anis; Mohamed, Adel Elsayed Ahmed; Nishikawa, Yoshifumi. |
| Neospora caninum and Cryptosporidium parvum are intracellular protozoan parasites that are distributed worldwide and of major economical concern in cattle industry. N. caninum can cause abortion storms and high culling rates, whereas C. parvum has zoonotic implications and can cause diarrhea in calves. There are currently no data on the prevalence of neosporosis and cryptosporidiosis in humans or animals in southern Egypt. Prevalence of these two infections was determined in a sample of cattle from two different areas in southern Egypt, Sohag and Qena, using enzyme-linked immunosorbent assay. A total 301 cattle were sampled, of which 18.9% were positive for N. caninum, 35.9% were positive for C parvum and 10.0% were positive for both. Geographical location... |
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Palavras-chave: Cattle; Cryptosporidium parvum; Neospora caninum; ELISA; Egypt. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4531 |
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| Registros recuperados: 32 | |
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