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Flassch, Jean-pierre; Barret, Jean; Mazurie, Joseph; Maes, P; Nicolas, J; Noel, Thierry; Paillard, C; Lepennec, M. |
A national program started in 1989 to drive researches on Ruditapes philippinarum brown ring disease correlated with a vibrio called P1. The researches have concerned epidemiological surveys on spats and adults, treatment methods, diagnostic techniques, and anatomical and functional studies about the vibrio-clam relations. The transmissions of the disease appeared mainly related to contaminated manila clam seeds, with secondary horizontal propagation. The brown ring formation appeared to be caused by a periostracum deterioration. Taking into account some of these results, an adapted cultural technique including special care of seeds and decreased densities was proposed to the farmers. |
Tipo: Text |
Palavras-chave: ANE; France; Brittany Ruditapes philippinarum Bivalvia Marine molluscs Vibriosis Research programmes Adults Spat Diseases Clam culture Rearing. |
Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/1992/acte-925.pdf |
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Choquet, Gwenaelle; Soudant, P; Lambert, C; Nicolas, Jean-louis; Paillard, C. |
Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V tapetis compared to that of V splendidus and V pectenicida suggests a specific pathogenicity... |
Tipo: Text |
Palavras-chave: Cytotoxicity; Flow cytometry; Vibrio tapetis; Ruditapes philippinarum; Bivalve hemocytes; Pathogenicity. |
Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00000/10761/7590.pdf |
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Maes, P; Paillard, C. |
The symptom which characterizes the brown ring disease (BRD) in Manilla clam Ruditapes philippinarum has been observed in other Bivalvia species (Veneridae and Pectinidae). Vibrio P1, responsible of the BRD in R. philippinarum , has been detected in other Veneridae which were carrying the symptom. In mussel and oysters which are cultivated in Europe, the symptom has never been reported and Vibrio P1 has not been detected. Experimental contaminations of bivalves of commercial interest with the pathogen showed a clear Vibrio P1 sensitivity of European clam R. decussatus ; this latter seems however to be less sensitive than the Manilla clam. |
Tipo: Text |
Palavras-chave: Ruditapes philippinarum; Bivalvia; Diseases; Marine molluscs; Pathology; Vibriosis. |
Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/1992/acte-926.pdf |
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