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Protocole standard pour la comparaison et le testage des caractéristiques de différents lots d’Artémia 5
Pham, Dominique.
This biotechnical paper describes the material and method used to evaluate different Artemia batches at the LAC Ifremer in New Caledonia.
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Ano: 2004 URL: http://archimer.ifremer.fr/doc/00119/23004/20835.pdf
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Salinité et confort physiologique - Application pratique en élevage larvaire 5
Pham, Dominique; Mailliez, Jean-rene; Peignon, Jean-marie; Broutoi, Francis; Marteau, Anne-laure; Wabete, Nelly.
The caledonian blue shrimp Litopenaeus stylirostris is the second largest export commodity in New Caledonia. This industry is based on 19 production farms but also four hatcheries, two feed production units and two packing plants. The maximum production was reached in 2005 with 2400 tons. Supply of juveniles by hatchery is the bottle neck of the production chain because this species is not present in the natural environment. Recent problems of larval survival led some scientists to work on eco-physiology to propose adaptations of larval rearing protocol. Salinity of the rearing environment is taken as an example to study the influence of abiotic parameters on the animal physiology according to its development stage. The influence of salinity on survival...
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Ano: 2011 URL: http://archimer.ifremer.fr/doc/00115/22658/20603.pdf
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Projet Déduction 2007-2010. Bilan par action et annexes 5
Beliaeff, Benoit; Laugier, Thierry; Chim, Liet; Coatanea, Denis; Della Patrona, Luc; Goyard, Emmanuel; Herlin, Jose; Labreuche, Yannick; Lemaire, Pierrette; Lemonnier, Hugues; Morvan, Patrice; Patrois, Jacques; Peignon, Jean-marie; Pham, Dominique; Soulard, Benoit; Wabete, Nelly; Walling, Emilie; Ansquer, Dominique; Castex, Mathieu; Lucas, Ronan; Reynaud, Yann; Ifremer, LEAD-NC,.
Ce document constitue le bilan détaillé des résultats du projet DEDUCTION (2007-2010) , action par action. Le projet DEDUCTION "DEveloppement DUrable de la Crevetticulture, Traitement de l’Information et Observatoire du système en Nouvelle-Calédonie" est un projet de recherche et de développement en soutien à la filière crevetticole néo-calédonienne. C'est un programme très horizontal et pluridisciplinaire en poursuivant et développant les ouvertures scientifiques, techniques et thématiques établies à la faveur de DESANS. Son ossature s’articule autour de 4 actions : 3 actions de recherche et une action transversale devant faciliter les interfaces entre la recherche et sa finalité, entre les chercheurs et les partenaires locaux (GFA, Provinces,...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00199/31006/35663.pdf
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Cross breeding of different domesticated lines as a simple way for genetic improvement in small aquaculture industries: Heterosis and inbreeding effects on growth and survival rates of the Pacific blue shrimp Penaeus (Litopenaeus) stylirostris 5
Goyard, Emmanuel; Goarant, Cyrille; Ansquer, Dominique; Brun, Pierre; De Decker, Sophie; Dufour, Robert; Galinie, C; Peignon, Jean-marie; Pham, Dominique; Vourey, Elodie; Harache, Yves; Patrois, Jacques.
Two populations of the Latin American shrimp Penaeus (Litopenaeus)stylirostris domesticated in Hawaii and in New Caledonia were previously shown to be genetically differentiated and proven highly inbred. In New Caledonia, where different Vibriosis affect shrimp production and antibiotic use is banned in growing ponds, the Hawaiian population was introduced to increase the allelic variability available for local shrimp farmers and start a genetic improvement program. Growth and survival rates of the two pure populations and the two-way F-1-hybrids obtained by breeding Hawaiian animals with New Caledonian animals were assessed in several simple experiments (earthen ponds, floating cages and experimental infection challenges) during two years on two...
Tipo: Text Palavras-chave: Vibriosis; Survival; Growth; Penaeus Litopenaeus stylirostris; Shrimp; Cross breeding; Genetic improvement.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4316.pdf
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Evaluation of floating cages as an experimental tool for marine shrimp culture studies under practical earthen pond condition 5
Castex, Mathieu; Chim, Liet; Lemaire, Pierrette; Wabete, Nelly; Pham, Dominique.
Scientific and technical studies carried out under semi-intensive farming conditions, with large earthen ponds (Fig 1), to assess the effects of treatments on zootechnical results are highly complex due to the many logistical, economic and/or experimental constraints involved. In our study, we evaluated the use of floatingcages (Paquotte et al., 1998) immersed in earthen ponds as an experimental tool in order to design future powerful and economical expe-riments under semi-intensive farming conditions.
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Ano: 2009 URL: http://archimer.ifremer.fr/doc/00197/30872/29240.pdf
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Floating cages as an experimental tool for shrimp culture studies: first attempts to check their reliability 5
Pham, Dominique; Castex, Mathieu; Wabete, Nelly; Lemaire, Pierrette; Brun, Pierre.
The first aim of this study was to evaluate on a technical basis the rearing of shrimps in floating cages set up in earthen ponds. Shrimps reared in and outside the cages presented comparable growths and survival rates. Then it was concluded that shrimp culture in floating cages did not present zootechnical biases comparing to pond culture. The second objective of this study was to assess the present method as a statistical point of view. We found that for expected differences of 20% to the control mean, 3 and 6 floating cages per treatment will be reasonable to determined statistical differences for growth parameters and survival. Furthermore, we showed a significant pond effect as regard as survival and growth between both sets of cages. This results...
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Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-4100.pdf
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Salinity tolerance, ontogeny of osmoregulation and zootechnical improvement in the larval rearing of the Caledonian Blue Shrimp, Litopenaeus stylirostris (Decapoda, Penaeidae) 5
Pham, Dominique; Charmantier, Guy; Wabete, Nelly; Boulo, Viviane; Broutoi, Francis; Mailliez, Jean-rene; Peignon, Jean-marie; Charmantier-daures, Mireille.
The ontogeny of osmoregulationwas investigated in Litopenaeus stylirostris by studying salinity tolerance and osmoregulatory capacity. Shrimp at different larval and postlarval stages were exposed to various salinities and survival was monitored for 24 h. Survival rates exceeded 80% at salinity over 25 ppt (750 mOsm.kg(-1)) at all the stages. At salinities below to 25 ppt, salinity tolerance was higher in nauplii and zoeae than in mysis larvae. Postlarval stages were able to withstand lower salinities, e.g. 6.0 ppt (176 mOsm.kg(-1)) at PL9 stage, but they were more sensitive than larvae to salinities over 35 ppt (1035 mOsm.kg(-1)). Zoea and mysis larvae slightly hyper-regulated at all tested salinities. After metamorphosis, postlarvae progressively...
Tipo: Text Palavras-chave: Penaeid; Litopenaeus stylirostris; Ontogeny; Osmoregulation; Growth.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00107/21843/20058.pdf
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DEDUCTION : A research project for shrimp farming sustainability in New-Caledonia 5
Beliaeff, Benoit; Chim, Liet; Della Patrona, Luc; Goyard, Emmanuel; Herlin, Jose; Labreuche, Yannick; Walling, Emilie; Ansquer, Dominique; Brun, Pierre; Castex, Mathieu; Coatanea, Denis; Courties, Claude; De Lorgeril, Julien; Dufour, Robert; Frappier, Julie; Goarant, Cyrille; Huber, Matthias; Lemaire, Pierrette; Lemonnier, Hugues; Loubersac, Lionel; Le Roux, Frederique; Lucas, Ronan; Patrois, Jacques; Peignon, Jean-marie; Pham, Dominique; Ramage, Yannick; Soulard, Benoit; Vic, Marlene; Vourey, Elodie; Wabete, Nelly.
New Caledonian shrimp farming feasibility studies started in the mid-seventies. Today ca. 2000 tons of Litopenaeus stylirostris, introduced in 1978, are semi-intensively produced each year. Since fifteen years, production has been impacted by two seasonal vibriosis, the “Summer” syndrome caused by Vibrio nigripulchritudo in relation to pond bottom and water column quality, and the “Winter Syndrome” caused by Vibrio penaeicida in relation to drastic temperature drops . With the ambition of elucidating processes possibly leading to mortality outbreaks in ponds, Ifremer has been conducting a research program since 2003 under the DESANS project (2003-2006) and the present DEDUCTION project, equally funded by the South and North Provinces and by the Government...
Tipo: Text Palavras-chave: Shrimp farming; Technical and scientific support; Vibriosis; Water quality; Pathology; Ecophysiology; Genetics; Zoosanitary monitoring and procedures; Multifactorial and multidisciplinary approach.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00143/25438/23591.pdf
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Phénomène des branchies orange dans les élevages de crevettes en Nouvelle-Calédonie 5
Lemonnier, Hugues; Barri, Kiam; Pham, Dominique; Wabete, Nelly; Boulo, Viviane; Lignot, Jean-herve; Royer, Florence; Hubert, Morgane; Blockmans, Bernard; Pierrot, Thomas; Laugier, Thierry; Tostin, Nathalie.
L'aquaculture est une activité importante du paysage économique de la Nouvelle-Calédonie., La crevette Bleue du Pacifique, Litopenaeus stylirostris est aujourd’hui l’espèce cible, élevée en semi-intensif, dans des bassins de terre de 4 à 10 hectares. Une coloration orange prononcée au niveau des branchies, comparable à celle observée suite à un stress hypoxique alors qu’aucun déficit en oxygène n’est constaté, est apparue récemment engendrant une décote du produit à la vente et donc un risque économique supplémentaire pour cette filière déjà fragilisée par des mortalités à vibrioses. Le phénomène se déclare à un poids moyen des animaux compris entre 10 et 18 g pour une durée moyenne d’élevage de 70 jours. L'intensité de la couleur évolue avec le stade de...
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00248/35909/34462.pdf
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Selection and characterization of potential probiotic bacteria for Litopenaeus stylirostris shrimp hatcheries in New Caledonia 5
Pham, Dominique; Ansquer, Dominique; Chevalier, Anne; Dauga, Clement; Peyramale, Aude; Wabete, Nelly; Labreuche, Yannick.
In New Caledonia, shrimp hatcheries are confronted with mass mortality in the larval stages, a phenomenon poorly understood as no specific causative agent has been identified. This has resulted in an excessive use of prophylactic antibiotics, although their adverse effects in aquaculture are notorious. The present work was thus aimed at selecting potential probiotic strains for penaeid hatcheries. From a pool of more than 400 marine bacterial isolates sampled from the local marine environment seven strains exhibited in vitro antagonistic activity towards Vibrio harveyi. These isolates were characterized both phenotypically and genotypically using a biochemical approach and 16S rDNA sequencing. Six out of these seven strains were found to belong to the...
Tipo: Text Palavras-chave: Shrimp; Litopenaeus stylirostris; Probiotic; Hatchery; Pseudoalteromonas; Immune response.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00190/30129/28591.pdf
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Contribution à l'amélioration des survies et performances de reproduction de L. stylirostris en saison fraîche en Nouvelle-Calédonie 5
Pham, Dominique; Wabete, Nelly; Lemaire, Pierrette; Mailliez, Jean-rene; Broutoi, Francis; Chim, Liet.
Since 1993, the New Caledonian shrimp industry has been faced with cold season mortality outbreaks of multifactorial origin. This pathology called “Syndrome 93” affects as well animals in grow-out ponds as broodstock after their transfer in maturation room, with up to 35% of mortality in 48 hours. From literature data and physiological studies on Litopenaeus stylirostris, several combinations of three abiotic parameters (temperature, salinity and feeding) have been tested during the breeders stocking period. This research allowed us to lower stress factors impact and to propose a transfer protocol which leads to an optimal physiological comfort of the animal. Results showed that survival rate is improved and spawning rates are twice higher. These findings...
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Ano: 2008 URL: http://archimer.ifremer.fr/doc/00118/22970/20800.pdf
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Caractéristiques des Artemia d’origine russe 5
Pham, Dominique.
New Artemia strain from Russia are now available on the market. They have been evaluated by comparison with Artemia from Great Salt Lake which are routinely used at the LAC hatchery,. Five criteria were compared and all of them were in favour of the American strain with 191 833 nauplii per gram while the Russian ones only gave 102 667 nii per gram. Another important criteria when using Artemia was hatching time which turned out to be longer for the Russian.
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Ano: 2004 URL: http://archimer.ifremer.fr/doc/00119/23005/20836.pdf
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A soft technology to improve survival and reproductive performance of Litopenaeus stylirostris by counterbalancing physiological disturbances associated with handling stress 5
Wabete, Nelly; Chim, Liet; Pham, Dominique; Lemaire, Pierrette; Massabuau, J.
The consequences of handling stress (fishing, transfer, eyestalk ablation) on shrimp broodstock are poorly documented. The weakness of farmed shrimp, Litopenaeus stylirostris, during winter is a major problem in New Caledonia, because of seasonal climate (tropical-sub-temperate). The transfer of broodstock in winter from earthen outdoor ponds to indoor maturation tanks in the hatchery (T=20 degrees C, Salinity=35 parts per thousand, fed shrimp) usually leads, after 48 h, to high mortality (up to 70%). Eyestalk ablation to induce ovarian maturation in females leads to further mortality. Starting from a background analysis of physiological disturbances (initial osmoregulatory imbalance) associated with handling stress (Wabete, N., Chim, L., Lernaire, P.,...
Tipo: Text Palavras-chave: Eyestalk ablation; Spawning; Handling stress; Broodstock; Litopenaeus stylirostris; Shrimp.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1880.pdf
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Probiotic P-acidilactici application in shrimp Litopenaeus stylirostris culture subject to vibriosis in New Caledonia 5
Castex, Mathieu; Chim, Liet; Pham, Dominique; Lemaire, Pierrette; Wabete, Nelly; Nicolas, Jean-louis; Schmidely, P; Mariojouls, C.
We studied the effects of a lactic acid bacterium, Pediococcus acidilactici (strain MA 18/5M, CNCM), as a dietary probiotic on growth performance and some nutritional and microbiological aspects of the shrimp Litopenaeus stylirostris. Experimental shrimp culture was carried out over 10 weeks, using floating cages of 14 m(2) each set in earthen ponds, in a New Caledonia farm affected by "Summer syndrome", a septicaemic vibriosis caused by Vibrio nigripulchritudo. The experiment design testing probiotic-coated pellets against control was replicated in two earthen ponds. High mortalities, characteristic of Summer syndrome, were observed during the first 2 weeks of the trial. The probiotic improved production in the treated cages from both ponds with increases...
Tipo: Text Palavras-chave: Vibriosis; Floating cages; Litopenaeus stylirostris; Pediococcus acidilactici; Probiotic.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4079.pdf
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Ontogeny of osmoregulation in the Pacific blue shrimp, Litopenaeus stylirostris (decapoda, penaeidae): Deciphering the role of the Na+/K+-ATPase 5
Pham, Dominique; Charmantier, Guy; Boulo, Viviane; Wabete, Nelly; Ansquer, Dominique; Dauga, Clement; Grousset, Evelyse; Labreuche, Yannick; Charmantier-daures, Mireille.
The role of the main ion transporting enzyme Na +/K + − ATPase in osmoregulation processes was investigated in Litopenaeus stylirostris. The development and localization of the osmoregulation sites were studied during ontogenesis by immunodetection of Na+ K+-ATPase using monoclonal antibodies and transmission electron microscopy (TEM). Osmoregulation sites were identified as the pleurae and branchiostegites in the zoeae and mysis stages. In the subsequent post-metamorphic stages the osmoregulatory function was mainly located in the epipodites and branchiostegites and osmotic regulation was later detected in the gills. The presence of ionocytes and microvilli in these tissues confirmed their role in ionic processes. The complete open reading frame of the...
Tipo: Text Palavras-chave: Penaeid; Litopenaeus stylirostris; Ontogeny; Osmoregulation; Na+/K+-ATPase; RNA interference.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00311/42176/41477.pdf
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Whole-Genome Sequence of Pseudoalteromonas sp. NC201, a Probiotic Strain for Litopenaeus stylirostris Hatcheries in New Caledonia 5
Sorieul, Louis; Rückert, Christian; Al-dilaimi, Arwa; Winkler, Anika; Kalinowski, Jörn; Pham, Dominique; Wabete, Nelly; Boulo, Viviane.
The marine bacterium Pseudoalteromonas sp. strain NC201 has shown probiotic potential in Litopenaeus stylirostris rearing. In this study, the complete genome of NC201 was sequenced. This genome consists of a chromosome (4.13 Mb) and a chromid (1.24 Mb). The genome contains gene clusters coding for antibacterial peptides and secondary metabolites.
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00512/62329/66586.pdf
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Amélioration génétique expérimentale de la crevette d'élevage de Nouvelle-Calédonie : Sélection d'une population de L. stylirostris résistante à la bactérie pathogène Vibrio penaeicida. Rapport final pour le Ministère de l'Outre-Mer 5
Goyard, Emmanuel; Goarant, Cyrille; Bachere, Evelyne; De Lorgeril, Julien; Mugnier, Chantal; Ansquer, Dominique; Broutoi, Francis; Brun, Pierre; Imbert, Frederic; Justou, Carole; Maillez, Jean-rene; Patrois, Jacques; Pham, Dominique; Peignon, Jean-marie.
The New-Caledonian shrimp industry is based on the controlled reproduction of the shrimp Litopenaeus stylirostris, a species which was introduced in the 80s. The major difficulty to which the industry has been faced for 10 years is the occurrence of the "syndrome 93", which corresponds to mortality phases when the temperature falls down in April-May-June. This mortality is associated to the pathogenic bacteria Vibrio penaecida and is expressed at different levels which are variable from year ta year and from pond to pond. No resistance to this pathology has been developed spontaneously. This is likely due to the protocole used to rear spawners, which does not allow to implement an efficient selective pressure at each generation An experimental selection...
Tipo: Text Palavras-chave: Amélioration génétique; P. styliroslris; Résistance; Syndrome 93; Lyzozyme; Genetic improvement; P. styliroslris; Résistance; Syndrome 93; Lyzozyme.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00109/22020/19643.pdf
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Evaluation of floating cages as an experimental tool for marine shrimp culture studies under practical earthen pond conditions 5
Chim, Liet; Castex, Mathieu; Pham, Dominique; Brun, Pierre; Lemaire, Pierrette; Wabete, Nelly; Schmidely, P; Mariojouls, C.
The New Caledonia blue shrimp Litopenaeus stylirostris is commercially produced under semi-intensive rearing conditions. The size of the farming earthen ponds (5–10 ha) and the subsequent production constraints make it difficult to use them as experimental units for random experiments. Moreover, since every pond has its own characteristics, ponds' effects cannot be ruled out, thus making it hard to define true replicates. In order to design future experiments under conditions resembling those used in production, we evaluated the possible use of floating cages as experimental units with the aim of assessing treatment effects with a reasonable statistical power. To this end, two sets of floating cages were placed respectively in two different ponds in a...
Tipo: Text Palavras-chave: Statistical power; Replicates; Pond experimentation; Floating cages; Litopenaeus stylirostris.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4363.pdf
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Grow-out of sandfish Holothuria scabra in ponds shows that co-culture with shrimp Litopenaeus stylirostris is not viable 5
Bell, J; Agudo, N; Purcell, S; Blazer, P; Simutoga, M; Pham, Dominique; Della Patrona, Luc.
We examined the potential for producing the large numbers of sandfish (Holothuria scabra) needed for restocking programmes by co-culturing juveniles with the shrimp Litopenaeus stylirostris in earthen ponds. Our experiments in hapas within shrimp ponds were designed to detect any deleterious effects of sandfish on shrimp, and vice versa. These experiments showed that a high stocking density of juvenile sandfish had no significant effects on growth and survival of shrimp. However, survival and growth of sandfish reared with shrimp for 3 weeks were significantly lower than for sandfish reared alone. Increased stocking density of shrimp also had a significant negative effect on survival and/or growth of sandfish. A grow-out trial of juvenile sandfish in...
Tipo: Text Palavras-chave: Shrimp; Sea cucumbers; Sandfish; Pond culture; Predation; Co culture.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3590.pdf
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Amélioration génétique pour la résistance au Syndrome 93 : bilan de 5 générations de sélection expérimentale 5
Goyard, Emmanuel; Ansquer, Dominique; Brun, Pierre; De Decker, Sophie; Dufour, Robert; Goarant, Cyril; Patrois, Jacques; Peignon, Jean; Pham, Dominique.
Selective breeding of shrimp for viral resistance is established for species like L. vannamei and L. stylirostris. However, selection for bacterial infection resistance is not documented. In New Caledonia, "Syndrome 93" is a vibriosis that affects cultured shrimps during the cool season with survival from 15 to 30%. Current practises for breeders rearing at low densities do not improve the resistance of the offsprings mainly because selection pressures for "Syndrome 93" are low. Even after the fifth generation, survival improvement is not significant.
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Ano: 2005 URL: http://archimer.ifremer.fr/doc/00117/22848/20658.pdf
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