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Registros recuperados: 54 | |
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Guesdon, Stephane; Travers, Marie-agnes; Hervio Heath, Dominique; Derrien, Annick; Genauzeau, Sylvie; Schmitt, Anne; Quintric, Laure; Leroi, Laura; Pepin, Jean-francois. |
Since the spring of 2014, the mussel farming of Charente-Maritime and Vendée (French Atlantic coast) has experienced an unprecedented crisis. Mass mortality events of blue mussel (M. edulis) have affected livestock areas, decimating up to 100% of production in some farming areas. Investigation for infectious agents in moribund mussels revealed presence of bacteria capable of inducing mortalities. In this context, the MORBLEU research project (DPMA-Ifremer agreement) was initiated to explore potentially aggravating factors, associated or correlated with mortality of mussels. The aim of the present study is to support the hypothesis that these mortality episodes can be linked to an evolution of microbial communities. Based on historical data from Ifremer... |
Tipo: Text |
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Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00415/52696/53566.pdf |
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Segarra, Amelie; Faury, Nicole; Haffner, Philippe; Pepin, Jean-francois; Tourbiez, Delphine; Trancart, Suzanne; Travers, Marie-agnes; Renault, Tristan. |
Le premier cas d'infection à virus de type herpès a été rapporté en 1972, aux Etats Unis, chez l'huître américaine, Crassostrea virginica (Farley et al, 1972). A partir de 1991, des mortalités massives associées à la détection de virus de type herpes ont été observées chez plusieurs espèces de bivalves marins, à différents stades de développement (essentiellement chez les larves et le naissain) et dans différentes régions du monde. Sur la base d’observation en microscopie électronique à transmission, ces virus présentaient des caractéristiques morphologiques identiques à celles des Herpesvirus (Roizman, 1982). Les connaissances acquises concernant la taille, la structure et le séquençage du génome d’un virus infectant les larves d’huîtres creuses en... |
Tipo: Text |
Palavras-chave: Herpès virus OsHV-1; Expression gènes; Huître creuse; Crassostrea gigas. |
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00195/30627/29076.pdf |
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Azema, Patrick; Travers, Marie-agnes; Benabdelmouna, Abdellah; Degremont, Lionel. |
French production of the Pacific cupped oyster, Crassostrea gigas, is currently threatened by two pathogens, OsHV-1 and V. aestuarianus. While oysters selected for their higher resistance to OsHV-1 are now available for the industry, the impact of V. aestuarianus on such oysters is unknown, especially for triploids. In addition, experimental infection has used the virus or the bacteria alone, but there have been no investigations of dual exposure to these pathogens. This study is the first report of single or dual exposure in spat (Spat1 and Spat2), juvenile and adult naïve oysters. For each of the two stocks evaluated, unselected oysters and oysters selected for their higher resistance to OsHV-1 infection were tested, as well as their triploid siblings of... |
Tipo: Text |
Palavras-chave: Vibrio aestuarianus; OsHV-1; Dual exposure; Ploidy; Selection; Crassostrea gigas. |
Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00347/45835/45918.pdf |
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Haenen, O. L. M.; Fouz, B.; Amaro, C.; Isern, M. M.; Mikkelsen, H.; Zrncic, S.; Travers, Marie-agnes; Renault, Tristan; Wardle, R.; Hellstrom, A.; Dalsgaard, I.. |
Aquaculture in brackish and marine water is growing worldwide (FAO, 2014), including new cultured species. Various Vibrio infections occur, and cause significant problems, in fish, crustacean and shellfish (Austin & Austin, 2007. Vibrio anguillarum, V. salmonicida, V. ordalii and V. vulnificus are among the pathogens that lead to the biggest losses in aquaculture all over the world (Toranzo et al. 2005, Sandlund et al., 2010; Sitjà-Bobadilla et al. 2014). |
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Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00211/32186/30615.pdf |
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Degremont, Lionel; Maurouard, Elise; Godfrin, Yoann; Travers, Marie-agnes; Morga, Benjamin; Lamy, Jean-baptiste; Benabdelmouna, Abdellah. |
Depuis 2012, le nombre de cas de mortalités d’huîtres creuses adultes (Crassostrea gigas) rapportés dans le cadre des réseaux de surveillance de l’Ifremer, et dans lesquels la bactérie Vibrio aestuarianus a été isolée, a fortement augmenté. Les objectifs de cette étude étaient (1) d’étudier la sensibilité des huîtres C. gigas à une infection à V. aestuarianus pour des lots diploïdes et triploïdes et (2) déterminer la réponse à la sélection pour une meilleure résistance à V. aestuarianus pour des huîtres C. gigas. Neuf stocks ont été utilisés, répartis en trois niveaux de sélection, chacun étant donc répliqué par trois pontes. Pour chaque stock, un lot diploïde (2n), un lot triploïde (3n_chim) produit par la rétention d’un globule polaire, et trois lots... |
Tipo: Text |
Palavras-chave: Vibrio aestuarianus; OsHV-1; Sélection; Résistance; Triploïdes; Huître creuse; Crassostrea gigas; Vibrio aestuarianus; OsHV-1; Selective breeding; Disease resistance Pacific oyster; Crassostrea gigas; Triploids. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00404/51530/52140.pdf |
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Azema, Patrick; Travers, Marie-agnes; De Lorgeril, Julien; Tourbiez, Delphine; Degremont, Lionel. |
Since 2008, the emergent virus OsHV 1μvar has provoked massive mortality events in Crassostrea gigas spat and juveniles in France. Since 2012, mortality driven by the pathogenic bacteria Vibrio aestuarianus has stricken market sized adults. A hypothesis to explain the sudden increase in mortality observed in France since 2012 is that selective pressure due to recurrent viral infections could have led to a higher susceptibility of adults to Vibrio infection. In our study, two OsHV-1-resistant lines (AS and BS) and their respective controls (AC and BC) were experimentally challenged in the laboratory to determine their level of susceptibility to V. aestuarianus infection. At the juvenile stage, the selected lines exhibited lower mortality (14 and 33%) than... |
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Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00299/41054/40215.pdf |
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Azema, Patrick; Lamy, Jean-baptiste; Boudry, Pierre; Renault, Tristan; Travers, Marie-agnes; Degremont, Lionel. |
Background In France, two main diseases threaten Pacific oyster production. Since 2008, Crassostrea gigas spat have suffered massive losses due to the ostreid herpesvirus OsHV-1, and since 2012, significant mortalities in commercial-size adults have been related to infection by the bacterium Vibrio aestuarianus. The genetic basis for resistance to V. aestuarianus and OsHV-1 and the nature of the genetic correlation between these two traits were investigated by using 20 half-sib sire families, each containing two full-sib families. For each disease, controlled infectious challenges were conducted using naïve oysters that were 3 to 26 months old. In addition, siblings were tested under field, pond and raceway conditions to determine whether laboratory... |
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Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00372/48350/48518.pdf |
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Barbosa-solomieu, Valerie; Renault, Tristan; Travers, Marie-agnes. |
Massive mortality outbreaks in cultured bivalves have been reported worldwide and they have been associated with infection by a range of viral and bacterial pathogens. Due to their economic and social impact, these episodes constitute a particularly sensitive issue in Pacific oyster (Crassostrea gigas) production. Since 2008, mortality outbreaks affecting C. gigas have increased in terms of intensity and geographic distribution. Epidemiologic surveys have lead to the incrimination of pathogens, specifically OsHV-1 and bacteria of the Vibrio genus, in particular Vibrio aestuarianus. Pathogen diversity may partially account for the variability in the outcome of infections. Host factors (age, reproductive status…) including their genetic background that has... |
Tipo: Text |
Palavras-chave: Pacific oyster; Spat; OsHV-1; Vibrio aestuarianus; Mortality. |
Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00274/38534/37051.pdf |
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Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garrigues, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre; Renault, Tristan. |
Mass mortality outbreaks (90-100%) in both juvenile and adult blue mussels were reported in 2014 along the Pertuis Charentais area of the French Atlantic coast. A series of studies were carried out to learn more about the phenomenon through detecting pathogens, describing environmental conditions and establishing the physiological status of the animals. Bacteria identif ied as belonging to the V. splendidus species were detected in dying mussels and seem able to cause mortality in blue mussels in the laboratory. Unusual environmental conditions (large amounts of fresh water, re-suspension of sediments during a series of storms, and a high renewal rate of specific water masses in Brittany’s Pertuis area) may be partially responsible for sparking the phenomen |
Tipo: Text |
Palavras-chave: Mortalités; Moules; Pertuis Charentais; Vibrio splendidus; Environnement; Mortality; Mussels; Pertuis Charentais; Vibrio splendidus; Environment. |
Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00312/42343/41682.pdf |
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Ben Henda, Yesmine; Laamari, Mariem; Lanneluc, Isabelle; Travers, Marie-agnes; Agogue, Helene; Arnaudin, Ingrid; Bridiau, Nicolas; Maugard, Thierry; Piot, Jean-marie; Sannier, Frederic; Bordenave-juchereau, Stephanie. |
The effect of 11 marine-derived cryptides was investigated on proliferation, differentiation and maturation of human white pre-adipocytes (HWP). They were all formerly identified as potent Angiotensin-Converting-Enzyme inhibitors.Val-Trp (VW),Val-Tyr (VY), Lys-Tyr (KY), Lys-Trp (KW), Ile-Tyr (IY), Ala-Pro (AP),Val-Ile-Tyr (VIY), Leu-Lys-Pro (LKP), Gly-Pro-Leu (GPL), Ala-Lys-Lys (AKK) and Val-Ala-Pro (VAP) were previously found in fish products and coproducts as well as other marine resources like wakame. Treatment with AP, VAP and AKK greatly affected viability of HWP during the proliferation period while KW and VW treatment reduced the number of viable cells during the differentiation stage. A GPL and IY incubation during the differentiation stage allowed... |
Tipo: Text |
Palavras-chave: Bioactive peptides; Obesity; Human white pre-adipocytes'; Proliferation; Differentiation; Adipocyte differentiation markers. |
Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00269/38014/36308.pdf |
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Travers, Marie-agnes; Mersni Achour, Rachida; Haffner, Philippe; Tourbiez, Delphine; Cassone, Anne-laure; Morga, Benjamin; Doghri, Ibtissem; Garcia, Celine; Renault, Tristan; Fruitier-arnaudin, Ingrid; Saulnier, Denis. |
Nine dominant bacterial isolates were obtained from different batches of Crassostrea gigas spat experiencing high mortality rates in a French experimental hatchery/nursery in 2007. Using phenotypic analysis combined with multilocus sequence analysis, the isolates were shown to be genetically close to the Vibrio tubiashii type strain. Based on (1) analyses of the recA gene sequences; (2) the results of DNA–DNA hybridization assays between 07/118 T2 (LMG 27884 = CECT 8426), which is a representative strain, and the V. tubiashii type strain (69%); and (3) phenotypic traits, the bacteria were classified in a group close to American V. tubiashii strain. Its virulence (70% of mortalities) and the toxicity of the extracellular products of 07/118 T2 was... |
Tipo: Text |
Palavras-chave: Crassostrea gigas Pathogenicity Extracellular products Polyphasic approach; Real time PCR. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00189/30028/28743.pdf |
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Travers, Marie-agnes; Sow, Cisse; Zirah, Severine; Deregnaucourt, Christiane; Chaouch, Soraya; Queiroz, Rayner M. L.; Charneau, Sebastien; Allain, Thibault; Florent, Isabelle; Grellier, Philippe. |
Giardiasis, currently considered a neglected disease, is caused by the intestinal protozoan parasite Giardia duodenalis and is widely spread in human as well as domestic and wild animals. The lack of appropriate medications and the spread of resistant parasite strains urgently call for the development of novel therapeutic strategies. Host microbiota or certain probiotic strains have the capacity to provide some protection against giardiasis. By combining biological and biochemical approaches, we have been able to decipher a molecular mechanism used by the probiotic strain Lactobacillus johnsonii La1 to prevent Giardia growth in vitro. We provide evidence that the supernatant of this strain contains active principle(s) not directly toxic to Giardia but able... |
Tipo: Text |
Palavras-chave: Giardiasis; Lactobacilli; Probiotics; Microbiota; Bile salts; Anti-giardial activity; Choloylglycine bile acid hydrolase. |
Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00351/46211/45906.pdf |
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Macneil, M. Aaron; Chapman, Demian D.; Heupel, Michelle; Simpfendorfer, Colin A.; Heithaus, Michael; Meekan, Mark; Harvey, Euan; Goetze, Jordan; Kiszka, Jeremy; Bond, Mark E.; Currey-randall, Leanne M.; Speed, Conrad W.; Sherman, C. Samantha; Rees, Matthew J.; Udyawer, Vinay; Flowers, Kathryn I.; Clementi, Gina; Valentin-albanese, Jasmine; Gorham, Taylor; Adam, M. Shiham; Ali, Khadeeja; Pina-amargos, Fabian; Angulo-valdes, Jorge A.; Asher, Jacob; Barcia, Laura Garcia; Beaufort, Oceane; Benjamin, Cecilie; Bernard, Anthony T. F.; Berumen, Michael L.; Bierwagen, Stacy; Bonnema, Erika; Bown, Rosalind M. K.; Bradley, Darcey; Brooks, Edd; Brown, J. Jed; Buddo, Dayne; Burke, Patrick; Caceres, Camila; Cardenosa, Diego; Carrier, Jeffrey C.; Caselle, Jennifer E.; Charloo, Venkatesh; Claverie, Thomas; Clua, Eric; Cochran, Jesse E. M.; Cook, Neil; Cramp, Jessica; D'Alberto, Brooke; De Graaf, Martin; Dornhege, Mareike; Estep, Andy; Fanovich, Lanya; Farabough, Naomi F.; Fernando, Daniel; Flam, Anna L.; Floros, Camilla; Fourqurean, Virginia; Garla, Ricardo; Gastrich, Kirk; George, Lachlan; Graham, Rory; Guttridge, Tristan; Hardenstine, Royale S.; Heck, Stephen; Henderson, Aaron C.; Hertler, Heidi; Hueter, Robert; Johnson, Mohini; Jupiter, Stacy; Kasana, Devanshi; Kessel, Steven T.; Kiilu, Benedict; Kirata, Taratu; Kuguru, Baraka; Kyne, Fabian; Langlois, Tim; Ledee, Elodie J. I.; Lindfield, Steve; Luna-acosta, Andrea; Maggs, Jade; Manjaji-matsumoto, B. Mabel; Marshall, Andrea; Matich, Philip; Mccombs, Erin; Mclean, Dianne; Meggs, Llewelyn; Moore, Stephen; Mukherji, Sushmita; Murray, Ryan; Kaimuddin, Muslimin; Newman, Stephen J.; Nogues, Josep; Obota, Clay; O'Shea, Owen; Osuka, Kennedy; Papastamatiou, Yannis P.; Perera, Nishan; Peterson, Bradley; Ponzo, Alessandro; Prasetyo, Andhika; Quamar, L. M. Sjamsul; Quinlan, Jessica; Ruiz-abierno, Alexei; Sala, Enric; Samoilys, Melita; Scharer-umpierre, Michelle; Schlaff, Audrey; Simpson, Nikola; Smith, Adam N. H.; Sparks, Lauren; Tanna, Akshay; Torres, Ruben; Travers, Marie-agnes; Van Zinnicq Bergmann, Maurits; Vigliola, Laurent; Ward, Juney; Watts, Alexandra M.; Wen, Colin; Whitman, Elizabeth; Wirsing, Aaron J.; Wothke, Aljoscha; Zarza-gonzalez, Esteban; Cinner, Joshua E.. |
Decades of overexploitation have devastated shark populations, leaving considerable doubt as to their ecological status1,2. Yet much of what is known about sharks has been inferred from catch records in industrial fisheries, whereas far less information is available about sharks that live in coastal habitats3. Here we address this knowledge gap using data from more than 15,000 standardized baited remote underwater video stations that were deployed on 371 reefs in 58 nations to estimate the conservation status of reef sharks globally. Our results reveal the profound impact that fishing has had on reef shark populations: we observed no sharks on almost 20% of the surveyed reefs. Reef sharks were almost completely absent from reefs in several nations, and... |
Tipo: Text |
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Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00644/75627/83233.pdf |
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Ben Cheikh, Yosra; Travers, Marie-agnes; Le Foll, Frank. |
The pathogenic strain V. splendidus 10/068 1T1 has previously been reported for its virulence to the blue mussel and for its capacity to alter immune responses. In this study, we expanded the knowledge on hemocyte-pathogen interactions by using in vitro and in vivo assays. V. splendidus 10/068 1T1 severely inhibited cell adhesion and acidic vacuole formation unlike the innocuous phylogenetically related V. splendidus 12/056 M24T1 which had no effect on these cell functions. Furthermore, the virulent bacteria decreased hemocyte viability (59% of viability after 24 h). Infection dynamics were explored by using a model based on water tank cohabitation with septic mussels infected by GFP-tagged V. splendidus 10/068 1T1. Experimental infections were... |
Tipo: Text |
Palavras-chave: Innate immunity; Bivalves; Vibrio; Infection; Experimental model; Hemocyte. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00400/51136/53772.pdf |
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Destoumieux‐garzón, Delphine; Canesi, Laura; Oyanedel, Daniel; Travers, Marie-agnes; Charrière, Guillaume; Pruzzo, Carla; Vezzulli, Luigi. |
n the marine environment, bivalve mollusks constitute habitats for bacteria of the Vibrionaceae family. Vibrios belong to the microbiota of healthy oysters and mussels, which have the ability to concentrate bacteria in their tissues and body fluids, including the hemolymph. Remarkably, these important aquaculture species respond differently to infectious diseases. While oysters are the subject of recurrent mass mortalities at different life stages, mussels appear rather resistant to infections. Thus, Vibrio species are associated to the main diseases affecting the worldwide oyster production. Here we review the current knowledge on Vibrio‐bivalve interaction in oysters (Crassostrea sp.) and mussels (Mytilus sp.). We discuss the transient versus stable... |
Tipo: Text |
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Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00628/74032/73360.pdf |
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Registros recuperados: 54 | |
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