|
|
|
Registros recuperados: 53 | |
|
| |
|
|
Geay, Florian; Darias, Maria J.; Santigosa, E.; Desbruyeres, Elisabeth; Quazuguel, Patrick; Zambonino, Jose-luis; Cahu, Chantal; Mazurais, David. |
It is known in vertebrates that endothelin-1 (ET-1) plays a key role ill morphogenesis whose expression level greatly influences the development of craniofacial malformations. In the Present study, the complete cDNA fragment encoding a precursor of endothelin-1, the preproendothelin-1 (PPET1), was cloned by RACE-PCR from European sea bass. The cDNA encoded a 199 amino acid polypeptide that was composed of the "mature" and "big" ET-1. Relative ET-1 expression levels were investigated in European sea bass larvae fed microparticulate diets containing the standard amount (0.08 g retinol/kg diet: group N) or all excess of retinoic acid which induces skeletal malformations (0.5 g retinol/kg diet: group RA). Real-time reverse transcriptor polymerase chain... |
Tipo: Text |
Palavras-chave: European sea bass; Retinoic acid; Morphogenesis; Gene expression; Endothelin 1. |
Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6172.pdf |
| |
|
|
Zambonino, Jose-luis; Mazurais, David; Dubuc, Alexia; Queau, Pierre; Vanderplancke, Gwenaelle; Servili, Arianna; Cahu, Chantal; Le Bayon, Nicolas; Huelvan, Christine; Claireaux, Guy. |
Ocean warming, eutrophication and consequent decrease in oxygen lead to smaller average fish size. Although such responses are well-known in an evolutionary context, involving multiple generations, it appears to be incompatible with current rapid environmental change. Rather, phenotypic plasticity could provide a means for marine fish to cope with rapid environmental changes. However, little is known about the mechanisms underlying plastic responses to environmental conditions that favour small phenotypes. Our aim was to investigate how and why European sea bass that had experienced a short episode of moderate hypoxia during their larval stage subsequently exhibited a growth depression at the juvenile stage compared to the control group. We examined... |
Tipo: Text |
Palavras-chave: Climate change; Digestive enzymes; Dicentrarchus labrax; Growth; Hypoxia; Respirometry. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00373/48407/48635.pdf |
| |
|
|
Kotzamanis, Y. P.; Gisbert, E.; Gatesoupe, Joel; Zambonino, Jose-luis; Cahu, Chantal. |
Two Fish Protein Hydrolysates (FPH) were incorporated into four diets prepared for start-feeding sea bass larvae, at two different levels (10% and 19% of total ingredients): a commercial FPH, CPSP, in which the molecular weight of the main fraction of soluble peptides (51%) was between 500-2500 Da, and an experimental FPH obtained by acidic silage of sardine offal, SH, with a main portion of soluble peptides (54%) ranging from 200 to 500 Da. The diet with 10% of the commercial FPH gave the best results in terms of growth, survival and intestinal development, as evaluated by the early activity of digestive enzymes in the brush border membrane (alkaline phosphatase and aminopeptidase N). This was related to the low level of Vibrio spp. counted in the larvae... |
Tipo: Text |
Palavras-chave: Pseudoalteromonas Bacillus Vibrio; Marinomonas Bacterial challenge microbiota Fish hydrolysate Digestive enzymes Development Fish larvae. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2145.pdf |
| |
|
|
Gerard, Andre; Thouard, Emmanuel; Zambonino, Jose-luis; Gaignon, Jean-louis; Antoine, Loic; Harache, Yves; Veron, Gerard; Perez, Jose. |
The repopulating of aquatic environments has long been based on the idea that in order to increase halieutic resources, one just had to artificially increase the number of fish in their early stages in marine or fresh water environments. Therefore, as the knowledge on the reproduction of some species and the mastery of their artificial reproduction was growing, the capacity to produce large amounts of eggs or fries from different fish or mollusc species became a reality. Controlled reproduction and the availability of large quantities of fertilized eggs, and then of larvae showing, in captivity, a greater survival capacity than naturally reproduced ones, lead to an increase of repopulating attempts through the release of fries or young fishes raised in... |
Tipo: Text |
Palavras-chave: Recommandation; Repeuplement; Milieu aquatique; Ressources halieutiques. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/rapport-6532.pdf |
| |
|
|
Omnes, Marie-helene; Lemerre, Charlotte; Le Bayon, Nicolas; Le Delliou, Herve; Zambonino, Jose-luis; Munschy, Catherine; Loizeau, Veronique. |
Persistent organic pollutants (POPs), such as PCBs (polychlorinated biphenyls) and PBDEs (polybrominated diphenyl ethers), are lipophilic and tend to bioaccumulate in cellular membranes and fat. The accumulation of lipids in oocytes during vitellogenesis, and subsequently in the eggs, favors the accumulation of POPs, and may affect the development of embryos. The effects of dietary exposure to POPs in Common sole Solea solea were evaluated on early life stages (ELS): the survival of sole embryos and features of embryonic development were assessed until hatching endpoint and compared to that of the control. No embryonic development was observed in PBDE eggs while hatching occurred in both control and PCB groups. In addition, several developmental... |
Tipo: Text |
|
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00125/23631/21475.pdf |
| |
|
|
Ducasse Cabanot, S; Zambonino, Jose-luis; Richard, N; Medale, Francoise; Corraze, Genevieve; Mambrini, M; Robin, Jean; Cahu, Chantal; Kaushik, Sadasivam; Panserat, S. |
For sustainable aquaculture, the removal of marine resource ingredients in fish diets is an important objective. While most studies focus on the replacement of fish oil by vegetable oil, little is known on the nutritional effects of presence (which corresponds to the control diet) or absence of dietary fish oil. We studied fatty acid composition of brush-border membranes and digestive enzyme activities of the intestine and measured the expression and activities of several enzymes involved in the hepatic intermediary metabolism of rainbow trout (Oncorhynchus mykiss) fed for 7 weeks with or without fish oil. The diets were pair-fed to ensure that fish fed either diet had comparable carbohydrate and protein intakes. Absence of fish oil significantly reduced... |
Tipo: Text |
Palavras-chave: Rainbow trout; Metabolism; Fish oil; Digestion; Aquaculture. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3506.pdf |
| |
|
|
Silva, Flavia; Nicoli, Jacques R.; Zambonino, Jose-luis; Le Gall, Marie-madeleine; Kaushik, Sadasivam; Gatesoupe, François-joel. |
The sustainable composition of diets of high nutritional value is of the utmost importance for intensive aquaculture. Digestion and absorption of nutrients depends on the activity of the digestive enzymes, in particular those located in the brush border membrane of enterocytes, which are responsible for the final stages of breaking down and absorption of nutrients. In the present study, the substitution of fish meal by lupin or rapeseed meal in the diet was evaluated on gilthead sea bream (Sparus aurata) and goldfish (Carassius auratus). The objectives were to compare the activities of intestinal brush border enzymes in both species fed the control and experimental diets. When gilthead sea bream were fed the vegetable diets, significantly lower activities... |
Tipo: Text |
Palavras-chave: Sparus aurata; Carassius auratus; Fish meal replacement; Brush border membrane; Digestive enzymes; Alkaline phosphatase; Aminopeptidase N; Maltase; Glutamyl transpeptidase. |
Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00009/11975/8679.pdf |
| |
|
| |
|
|
Zambonino, Jose-luis; Cahu, Chantal. |
During these last 20 years, many studies have focussed on the development of the digestive tract in marine fish larvae. Most of the studies aimed at acquiring knowledge on the optimal form of dietary supply for different nutrients, in order to formulate a compound diet able to totally replace live preys in the fish larvae feeding sequence. Consequently, most of the studies aimed at describing the effect of dietary adaptation on digestive enzymes profile, the morphology of the main organs, while others aspects of the physiology of the larvae digestive tract (gut hormones, intestinal transport...) were poorly investigated. This review reports the more recent data on dietary modulation of digestive enzymes with a particular emphasis, when possible, on the... |
Tipo: Text |
Palavras-chave: Nuclear receptors; Digestive enzymes; Fatty acids; Vitamins; Dietary proteins and lipids; Fish larvae. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3045.pdf |
| |
|
|
Darias, Maria J.; Zambonino, Jose-luis; Hugot, K; Cahu, Chantal; Mazurais, David. |
During the larval period, marine teleosts undergo very fast growth and dramatic changes in morphology, metabolism, and behavior to accomplish their metamorphosis into juvenile fish. Regulation of gene expression is widely thought to be a key mechanism underlying the management of the biological processes required for harmonious development over this phase of life. To provide an overall analysis of gene expression in the whole body during sea bass larval development, we monitored the expression of 6,626 distinct genes at 10 different points in time between 7 and 43 days post-hatching (dph) by using heterologous hybridization of a rainbow trout cDNA microarray. The differentially expressed genes (n = 485) could be grouped into two categories: genes that were... |
Tipo: Text |
Palavras-chave: Sea bass; Gene expression; Microarray; Development; Fish larvae. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4344.pdf |
| |
|
|
Geay, E; Santigosa I Culi, E; Corporeau, Charlotte; Boudry, Pierre; Dreano, Y; Corcos, L; Bodin, Nathalie; Kaushik, Sadasivam; Vandeputte, Marc; Zambonino, Jose-luis; Mazurais, David; Cahu, Chantal. |
Tipo: Text |
|
Ano: 2010 |
|
| |
|
| |
|
|
Chatelier, Aurelien; Mckenzie, David; Prinet, Aurelie; Galois, Robert; Robin, Jean; Zambonino, Jose-luis; Claireaux, Guy. |
Seabass were fed for 4 months with diets where the lipid was provided as either canola oil (CO), palm oil ( PO) or fish oil (FO), to generate diversity in their tissue fatty acid (FA) composition and investigate how this influenced major traits of exercise performance, cardiac performance and respiratory metabolism. In particular, based upon previous observations, we investigated the hypothesis that enriching the fish tissues with oleic and linoleic acids (OA, 18:1n-9 and LA, 18:2n-6, respectively) from the CO and PO diets would improve maximum exercise and cardiac performance, and increase aerobic metabolic scope. This proved to be the case; exercise respirometry on bass fitted with cardiac flow probes revealed that those fed CO and PO diets had a... |
Tipo: Text |
Palavras-chave: Diet; Fatty acid; Cardiovascular performance; Metabolism; Swimming; Dicentrarchus labrax; Seabass. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1860.pdf |
| |
|
|
Fernandez, I; Hontoria, F; Ortiz Delgado, J; Kotzamanis, Y; Estevez, A; Zambonino, Jose-luis; Gisbert, E. |
Several nutritional studies have found a direct effect of several vitamins in chondrogenic and osteogenic development during early life stages of marine fish species. In the present study, the effect of vitamin A (VA) in gilthead sea bream skeletogenesis was evaluated by means of four different dietary regimes (enriched rotifers) containing increasing levels of total VA (75,109, 188 and 723 ng total VA mg(-1) DW). Dietary treatments were offered to larvae during the rotifer-feeding phase (4-20 days after hatching), while later all groups were fed with Artemia nauplii and weaned onto the same inert diet. Different dietary doses of VA affected gilthead sea bream larval growth, survival, performance (maturation of the digestive system) and quality (incidence... |
Tipo: Text |
Palavras-chave: Deformities; Skeleton; Vitamin A; Larval quality; Sparus aurata; Gilthead sea bream. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4937.pdf |
| |
|
|
Seiliez, I; Bruant, J; Zambonino, Jose-luis; Kaushik, Sadasivam; Bergot, Pierre. |
The aim of the study was to determine the influence of dietary phospholipid (PL) levels on survival and development of first feeding gilthead sea bream (Sparus aurata) larvae. Larvae were fed from day 4 to 23 posthatching with an isoproteic and isolipidic formulated diet with graded levels of PL from 90-150 g kg(-1) dry matter (DM). A dietary PL content of more than 90 g kg(-1) DM seems to be necessary for sustaining growth of first feeding sea bream larvae. The survival rates of larvae fed the formulated diets (31-40% at day 23) were similar to those generally observed in marine aquaculture hatcheries with live prey feeding sequence. However, this high survival rate was not associated with high growth and the larvae showed, at the end of the study, a high... |
Tipo: Text |
Palavras-chave: Sparus aurata; Phospholipids; Larvae; First feeding; Development; Compound diet. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1869.pdf |
| |
|
|
Cahu, Chantal; Zambonino, Jose-luis. |
The production of fish larvae by hatchery shows an intensive increase for several species, but the specific nutritional requirements of larvae were poorly known until some years. We have conducted several experiments aimed to understand the specificities in larvae digestion, and to formulate a compound diet taking into account the differences compared to juveniles. The chosen species was the European sea bass, Dicentrarchus labrax, and the data were validated on different species. Larvae were fed live preys or experimental compound diet, especially formulated for the experiment. It appeared that pancreatic digestive enzymes, such as trypsin and amylase could be assayed in early ontogenesis, before mouth opening, and increase dramatically during the first... |
Tipo: Text |
Palavras-chave: Peptides; Phospholipid; Digestive enzymes; Nutrition; Larvae; Dicentrarchus labrax. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3332.pdf |
| |
|
|
Geay, Florian; Culi, E. Santigosa I.; Corporeau, Charlotte; Boudry, Pierre; Dreano, Y.; Corcos, L.; Bodin, Nathalie; Vandeputte, Marc; Zambonino, Jose-luis; Mazurais, David; Cahu, Chantal. |
Supplies of marine fish oils are limited, and continued growth in aquaculture production dictates that lipid substitutes in fish diets must be used without compromising fish health and product quality. In this study, the total substitution of a fish meal and fish oil by a blend of vegetable meals (corn, soybean, wheat and lupin) and linseed oil in the diet of European sea bass (Dicentrachus labrax) was investigated. Two groups of European sea bass were fed with fish diet (FD) or vegetable diet (VD) for 9 months. VD, totally deprived of eicosapentaenoate (EPA; 20:5n-3) and docosahexaenoate (DHA; 22:6n-3), revealed a nutritional deficiency and affected growth performance. Whilst VD induced a significant increase in fatty acid desaturase 2 (FADS2) and sterol... |
Tipo: Text |
Palavras-chave: Sea bass (Dicentrarchus labrax); FADS2 expression and activity; HUFA biosynthesis. |
Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00008/11929/8635.pdf |
| |
|
|
Gisbert, Enric; Villeneuve, Laure; Zambonino, Jose-luis; Quazuguel, Patrick; Cahu, Chantal. |
We evaluated the effects of dietary lipid class (phospholipid vs. neutral lipid) and level of n-3 long-chain PUFA (LC-PUFA) on the growth, digestive enzymatic activity, and histological organization of the intestine and liver in European sea bass larvae. Fish were fed from the onset of exogenous feeding at 7 to 37 d post-hatch with five isoproteic and isolipidic compound diets with different levels of EPA and DHA. Diet names indicated the percentage of EPA and DHA contained in the phospholipids (PL) and neutral lipids (NL), as follows: PL5, PL3, PL1, NL1, and NL3. Histological observations showed different patterns of lipid absorption and accumulation in the intestinal mucosa depending on the level and nature of the dietary lipid fraction. Fish fed high... |
Tipo: Text |
Palavras-chave: Histology; Digestive system; Phospholipid; Larvae; Dicentrarchus labrax; Dietary. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-929.pdf |
| |
|
|
Chatelier, Aurelien; Imbert, Nathalie; Zambonino, Jose-luis; Mckenzie, David; Bois, P. |
The present study employed a patch clamp technique in isolated seabass ventricular myocytes to investigate the hypothesis that oleic acid (OA), a mono-unsaturated fatty acid, can exert direct effects upon whole-cell barium currents. Acute application of free OA caused a dose-dependent depression of the whole-cell barium current that was evoked by a voltage step to 0 mV from a holding potential of -80 mV. The derived 50% inhibitory concentration (IC50) was 12.49 +/- 0.27 mu mol l(-1). At a concentration of 30 mu mol l(-1), OA significantly reduced the current density to about 45% of control values, but did not modify either the shape of the current-density voltage relationship or the apparent reversal potential. In addition, OA did not modify the voltage... |
Tipo: Text |
Palavras-chave: Fatty acid; Sea bass; Ventricular myocyte; L type calcium channel; Oleic acid. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2120.pdf |
| |
Registros recuperados: 53 | |
|
|
|