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| Registros recuperados: 23 | |
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| Priolli,Regina Helena Geribello; Pinheiro,José Baldin; Zucchi,Maria Imaculada; Bajay,Miklos Maximiliano; Vello,Natal Antonio. |
| In this study, simple sequence repeats (SSR) loci and pedigree data were used to investigate the genetic relationship in a group of 168 Brazilian soybean cultivars. Eighteen SSR loci produced an average of 5.06 alleles and a mean gene diversity of 0.58 for the cultivars studied. Genetic distance (GD) was determined using the modified Roger's Wright distance, and a final dendrogram was in agreement with the cultivar pedigree. A distance matrix based on the coefficient of parentage scores was also generated for the cultivars, which ranged from 0 to 1, with a mean of 0.18, whereas SSR-based genetic similarity (1- GD) ranged from 0.01 to 0.90, with a mean of 0.25. Mantel's Z test showed that the similarity matrices generated from both the data sets were low,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coefficient of parentage; Glycine max (L.) Merr.; Microsatellite markers. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300004 |
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| Oliveira,Eder Jorge; Pádua,Juliano Gomes; Zucchi,Maria Imaculada; Vencovsky,Roland; Vieira,Maria Lúcia Carneiro. |
| Microsatellites, or simple sequence repeats (SSRs), have been the most widely applied class of molecular markers used in genetic studies, with applications in many fields of genetics including genetic conservation, population genetics, molecular breeding, and paternity testing. This range of applications is due to the fact that microsatellite markers are co-dominant and multi-allelic, are highly reproducible, have high-resolution and are based on the polymerase chain reaction (PCR). When first introduced, the development of microsatellite markers was expensive but now new and efficient methods of repetitive sequence isolation have been reported, which have led to reduced costs and microsatellite-technology has been increasingly applied to several species,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellites; Molecular genetics; Genetic structure of populations. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200018 |
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| Perseguini,Juliana Morini Küpper Cardoso; Chioratto,Alisson Fernando; Zucchi,Maria Imaculada; Colombo,Carlos Augusto; Carbonell,Sérgio Augusto Moraes; Mondego,Jorge Mauricio Costa; Gazaffi,Rodrigo; Garcia,Antonio Augusto Franco; Campos,Tatiana de; Souza,Anete Pereira de; Rubiano,Luciana Benchimol. |
| A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger's modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AFLPs; Genetic structure; Genetic variability; Phaseolus vulgaris L.; SSRs. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100017 |
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| Abreu,Aluana Gonçalves; Priolli,Regina Helena Geribello; Azevedo-Filho,Joaquim Adelino; Nucci,Stella Maris; Zucchi,Maria Imaculada; Coelho,Ricardo Marques; Colombo,Carlos Augusto. |
| Acrocomia aculeata is a perennial, fruit-producing palm tree, native to tropical forests. Its fruits have spurred interest because of their significant potential for use in the cosmetic industry and as feedstock for biofuel. In the present study, the genetic structure and mating system in Acrocomia aculeata were analyzed, using eight nuclear microsatellite loci and samples from São Paulo and Minas Gerais states, Brazil. By means of Bayesian analysis, these populations were clustered into two or three groups. A high multilocus outcrossing rate suggests that outcrosses were predominant, although a certain degree of biparental inbreeding also occurred. Thus, although monoecious and self-compatible, there is every indication that A. aculeata bears a mixed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: SSR; Mating system. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100016 |
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| Gouvêa,Lígia Regina Lima; Rubiano,Luciana Benchimol; Chioratto,Alisson Fernando; Zucchi,Maria Imaculada; Gonçalves,Paulo de Souza. |
| Genetic diversity of 60 Hevea genotypes, consisting of Asiatic, Amazonian, African and IAC clones, and pertaining to the genetic breeding program of the Agronomic Institute (IAC), Brazil, was estimated. Analyses were based on phenotypic multivariate parameters and microsatellites. Five agronomic descriptors were employed in multivariate procedures, such as Standard Euclidian Distance, Tocher clustering and principal component analysis. Genetic variability among the genotypes was estimated with 68 selected polymorphic SSRs, by way of Modified Rogers Genetic Distance and UPGMA clustering. Structure software in a Bayesian approach was used in discriminating among groups. Genetic diversity was estimated through Nei's statistics. The genotypes were clustered... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genealogy; Genetic diversity; Hevea brasiliensis; Multivariate analysis; SSRs. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200018 |
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| Santos-Garcia,Melissa Oliveira; Toledo-Silva,Guilherme de; Sassaki,Rodrigo Possidonio; Ferreira,Thais Helena; Resende,Rosângela Maria Simeão; Chiari,Lucimara; Karia,Cláudio Takao; Carvalho,Marcelo Ayres; Faleiro,Fábio Gelape; Zucchi,Maria Imaculada; Souza,Anete Pereira de. |
| Stylosanthes species are important forage legumes in tropical and subtropical areas. S. macrocephala and S. capitata germplasm collections that consist of 134 and 192 accessions, respectively, are maintained at the Brazilian Agricultural Research Corporation Cerrados (Embrapa-Cerrados). Polymorphic microsatellite markers were used to assess genetic diversity and population structure with the aim to assemble a core collection. The mean values of H O and H E for S. macrocephala were 0.08 and 0.36, respectively, whereas the means for S. capitata were 0.48 and 0.50, respectively. Roger's genetic distance varied from 0 to 0.83 for S. macrocephala and from 0 to 0.85 for S. capitata. Analysis with STRUCTURE software distinguished five groups among the S.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stylosanthes; Tropical forage; Microsatellites; Genetic diversity; Core collection. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500020 |
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| Perseguini,Juliana Morini Küpper Cardoso; Silva,Gliciane Micaele Borges; Rosa,João Ricardo Bachega Feijó; Gazaffi,Rodrigo; Marçal,Jéssica Fernanda; Carbonell,Sérgio Augusto Morais; Chiorato,Alisson Fernando; Zucchi,Maria Imaculada; Garcia,Antonio Augusto Franco; Benchimol-Reis,Luciana Lasry. |
| Because of the continuous introduction of germplasm from abroad, some collections have a high number of accessions, making it difficult to explore the genetic variability present in a germplasm bank for conservation and breeding purposes. Therefore, the aim of this study was to quantify and analyze the structure of genetic variability among 500 common bean accessions to construct a core collection. A total of 58 SSRs were used for this purpose. The polymorphism information content (PIC) in the 180 common bean accessions selected to compose the core collection ranged from 0.17 to 0.86, and the discriminatory power (DP) ranged from 0.21 to 0.90. The 500 accessions were clustered into 15 distinct groups and the 180 accessions into four distinct groups in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular markers; Genetic diversity; Genetic structure; Microsatellites; Phaseolus vulgaris L. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100067 |
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| Alves-Pereira,Alessandro; Peroni,Nivaldo; Cavallari,Marcelo Mattos; Lemes,Maristerra R.; Zucchi,Maria Imaculada; Clement,Charles R.. |
| Abstract Although manioc is well adapted to nutrient-poor Oxisols of Amazonia, ethnobotanical observations show that bitter manioc is also frequently cultivated in the highly fertile soils of the floodplains and Amazonian dark earths (ADE) along the middle Madeira River. Because different sets of varieties are grown in each soil type, and there are agronomic similarities between ADE and floodplain varieties, it was hypothesized that varieties grown in ADE and floodplain were more closely related to each other than either is to varieties grown in Oxisols. We tested this hypothesis evaluating the intra-varietal genetic diversity and the genetic relationships among manioc varieties commonly cultivated in Oxisols, ADE and floodplain soils. Genetic results did... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Manihot esculenta; Microsatellites; Amazonian dark earths; Floodplain; Oxisols. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300468 |
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| Mulato,Bruno Mello; Möller,Milene; Zucchi,Maria Imaculada; Quecini,Vera; Pinheiro,José Baldin. |
| The objectives of this work were to investigate the genetic variation in 79 soybean (Glycine max) accessions from different regions of the world, to cluster the accessions based on their similarity, and to test the correlation between the two types of markers used. Simple sequence repeat markers present in genomic (SSR) and in expressed regions (EST-SSR) were used. Thirty SSR primer-pairs were selected (20 genomic and 10 EST-SSR) based on their distribution on the 20 genetic linkage groups of soybean, on their trinucleotide repetition unit and on their polymorphism information content. All analyzed loci were polymorphic, and 259 alleles were found. The number of alleles per locus varied from 2-21, with an average of 8.63. The accessions exhibit a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Molecular marker; Plant breeding; Plant germplasm; Polymorphism information content. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000300007 |
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| Santos,Carlos Antonio Fernandes; Rodrigues,Marciene Amorim; Zucchi,Maria Imaculada. |
| O objetivo deste trabalho foi avaliar a distribuição da variabilidade genética do umbuzeiro (Spondias tuberosa), no Semi-Árido brasileiro, por meio de marcadores AFLP, para subsidiar estratégias de prospecção e conservação da espécie. Foram analisados 68 indivíduos de umbuzeiro de 15 ecorregiões, pelo dendrograma UPGMA e pela dispersão em escala multidimensional (MDS), com o coeficiente de Jaccard de 141 bandas polimórficas de AFLP. A análise da variância molecular foi realizada pela decomposição total entre e dentro das regiões ecogeográficas. O dendrograma apresentou valor cofenético de 0,96, e o gráfico MDS apresentou 0,25 para a falta de ajustamento. A variabilidade genética do umbuzeiro foi estimada em 0,3138, o que indica grande variação entre os... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Spondias tuberosa; Análise multivariada; Análise de variância molecular; Dendrograma; Escala multidimensional. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000800013 |
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| Zucchi,Maria Imaculada; Pinheiro,José Baldin; Chaves,Lázaro José; Coelho,Alexandre Siqueira Guedes; Couto,Mansuêmia Alves; Morais,Lizz Kezzy de; Vencovsky,Roland. |
| This study was carried out to assess the genetic variability of ten "cagaita" tree (Eugenia dysenterica) populations in Southeastern Goiás. Fifty-four randomly amplified polymorphic DNA (RAPD) loci were used to characterize the population genetic variability, using the analysis of molecular variance (AMOVA). A phiST value of 0.2703 was obtained, showing that 27.03% and 72.97% of the genetic variability is present among and within populations, respectively. The Pearson correlation coefficient (r) among the genetic distances matrix (1 - Jaccard similarity index) and the geographic distances were estimated, and a strong positive correlation was detected. Results suggest that these populations are differentiating through a stochastic process, with restricted... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Myrtaceae; Cerrado; Tropical species; Genetic diversity. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005001000005 |
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| Aguiar,Ananda Virginia de; Moura,Nara Fernandes; Moura,Mara Fernandes; Zucchi,Maria Imaculada; Vencovsky,Roland; Chaves,Lázaro José. |
| O objetivo do trabalho foi relacionar a diversidade genética medida a partir de três diferentes marcadores moleculares, com a variação genética quantitativa de caracteres poligênicos, estimada em ensaio de progênies, sob condições controladas. As progênies, oriundas de dez subpopulações naturais de cagaiteira do sudeste de Goiás, foram avaliadas em experimento em blocos completos casualizados, com quatro repetições e uma planta por parcela. Foram estimadas a herdabilidade ao nível de média de progênies (h mi ²) e o coeficiente de variação genética (CVgi) de cada subpopulação para a altura da planta e o diâmetro do fuste, por quatro anos, bem como para as respectivas taxas de crescimento. Estimativas da diversidade gênica (Hei) e do índice de fixação (f i)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Correlação; Regressão múltipla; Marcadores moleculares; Caracteres quantitativos; Conservação genética. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452011000100021 |
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| Silvestre,Ellida de Aguiar; Costa,Márcio da Silva; Silva,Pollyana Oliveira da; Bajay,Miklos Maximiliano; Pinheiro,José Baldin; Zucchi,Maria Imaculada; Campelo,José Elivalto Guimarães; Britto,Fábio Barros. |
| The objective of this study was to characterize the genetic variability of Anglo-Nubian goats using microsatellite markers. The study was conducted using herds from four municipalities of Central-Northern Piauí (Teresina, José de Freitas, Campo Maior, and Angical), where technical information is scarce. Seven markers suggested by FAO were used (ILSTS11, McM527, INRA23, ETH10, OarfCB304, OarfCB48, and MAF209). The samples were genotyped using a 7% polyacrylamide gel. The average number of alleles per locus was 4.0, with observed and expected heterozygosity of 0.38 and 0.55, respectively. Few deviations from the Hardy-Weinberg equilibrium were observed for each population. Only two loci deviated significantly in two localities. The coefficient of gene... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Endogamy; Genetic structure; Microsatellite; Molecular markers. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015000400155 |
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| Gomes,Regina Lucia Ferreira; Costa,Marcones Ferreira; Alves-Pereira,Alessandro; Bajay,Miklos Maximiliano; Viana,João Paulo Gomes; Valente,Sérgio Emílio dos Santos; Lopes,Ângela Celis de Almeida; Zucchi,Maria Imaculada; Pinheiro,José Baldin. |
| ABSTRACT Some germplasm collections have a high number of accessions, which makes it difficult to explore the genetic variability present in the germplasm bank due to the redundancy and the difficulty of detailed analysis of all conserved accessions. Therefore, our study aimed to analyze the genetic diversity of 153 lima bean (Phaseolus lunatus) accessions for the purpose of constructing a core collection. Eleven SSRs were used for this purpose. The 153 lima bean accessions can be represented by low redundancy using a minimum of 34 accessions, thus representing 22 % of the size of the entire germplasm bank. The core collection had a higher Shannon diversity index and expected heterozygosity (1.906 and 0.811, respectively) than those presented by the entire... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Germplasm; Genetic diversity; Genetic structure; Microsatellite markers. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000201100 |
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| Registros recuperados: 23 | |
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