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| Registros recuperados: 2.463 | |
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| Viscardi,Lucas Henriques; Tovo-Rodrigues,Luciana; Paré,Pamela; Fagundes,Nelson Jurandi Rosa; Salzano,Francisco Mauro; Paixão-Côrtes,Vanessa Rodrigues; Bau,Claiton Henrique Dotto; Bortolini,Maria Cátira. |
| Abstract The FOXP subfamily is probably the most extensively characterized subfamily of the forkhead superfamily, playing important roles in development and homeostasis in vertebrates. Intrinsically disorder protein regions (IDRs) are protein segments that exhibit multiple physical interactions and play critical roles in various biological processes, including regulation and signaling. IDRs in proteins may play an important role in the evolvability of genetic systems. In this study, we analyzed 77 orthologous FOXP genes/proteins from Tetrapoda, regarding protein disorder content and evolutionary rate. We also predicted the number and type of short linear motifs (SLIMs) in the IDRs. Similar levels of protein disorder (approximately 70%) were found for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: FOXP2; Intrinsically disordered protein regions; Forkhead superfamily; Short linear motif; Molecular evolution. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100181 |
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| Uz,Gulsen; Sarikaya,Aysegul Topal. |
| Abstract Magnesium (Mg2+), an essential ion for cells and biological systems, is involved in a variety of cellular processes, including the formation and breakdown of microtubules. The results of a previous investigation suggested that as cells grow the intracellular Mg2+ concentration falls, thereby stimulating formation of the mitotic spindle. In the present work, we used a Mg2+-deficient Schizosaccharomyces pombe strain GA2, in which two essential membrane Mg2+ transporter genes (homologs of ALR1 and ALR2 in Saccharomyces cerevisae) were deleted, and its parental strain Sp292, to examine the extent to which low Mg2+ concentrations can affect mitotic spindle formation. The two S. pombe strains were transformed with a plasmid carrying a GFP-α2-tubulin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Magnesium; Microtubule; Mitosis; Mitotic spindle; Schizosaccharomyces pombe. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300459 |
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| Vasconcelos,Janaina Mota de; Móia,Lizomar de Jesus Maués Pereira; Amaral,Ivanete do Socorro Abraçado; Miranda,Esther Castello Branco Mello; CicaliseTakeshita,Louise Yukari; Oliveira,Layanna Freitas de; Mendes,Lilian de Araújo Melo; Sastre,Danuta; Tamegão-Lopes,Bruna Pedroso; Pedroza,Larysse Santa Rosa de Aquino; Santos,Sidney Emanuel Batista dos; Soares,Manoel do Carmo Pereira; Araújo,Marialva Tereza Ferreira de; Bandeira,Camila Lucas; Silva,Adriana Maria Paixão de Sousa da; Medeiros,Zilene Lameira de; Sena,Leonardo; Demachki,Samia; Santos,Eduardo José Melo dos. |
| Soroprevalence for Hepatitis C virus is reported as 2.12% in Northern Brazil, with about 50% of the patients exhibiting a sustained virological response (SVR). Aiming to associate polymorphisms in Killer Cell Immunoglobulin-like Receptors (KIR) with chronic hepatitis C and therapy responses we investigated 125 chronic patients and 345 controls. Additionally, 48 ancestry markers were genotyped to control for population stratification. The frequency of the KIR2DL2 and KIR2DL2+HLA-C Asp80 gene and ligand was higher in chronic infected patients than in controls (p < 0.0009, OR = 3.4; p = 0.001, OR = 3.45). In fact, KIR2DL3 is a weaker inhibitor of NK activity than KIR2DL2, which could explain the association of KIR2DL2 with chronic infection. Moreover,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HCV; KIR; HLA-C; Hepatitis C; KIR2DL2. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100004 |
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| Xue,Deming; Xue,Yang; Niu,Zhipeng; Guo,Xueqiang; Xu,Cunshuan. |
| Abstract 14-3-3 proteins play a vital part in the regulation of cell cycle and apoptosis as signaling integration points. During liver regeneration, the quiescent hepatocytes go through hypertrophy and proliferation to restore liver weight. Therefore, we speculated that 14-3-3 proteins regulate the progression of liver regeneration. In this study, we analyzed the expression patterns of 14-3-3 proteins during liver regeneration of rat to provide an insight into the regenerative mechanism using western blotting. Only four isoforms (γ, ε, σ and τ/θ) of the 14-3-3 proteins were expressed in regenerative liver after partial hepatectomy (PH). The dual effects, the significant down-regulation of 14-3-3ε and the significant up-regulation of 14-3-3τ/θ at 2 h after... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Liver regeneration; 14-3-3 proteins; Western blotting. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500855 |
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| Ohsaka,Yasuhito; Nishino,Hoyoku. |
| Research has been conducted to identify sequence polymorphisms of gene promoter regions in patients and control subjects, including normal individuals, and to determine the influence of these polymorphisms on transcriptional regulation in cells that express wild-type or mutant p53. In this study we isolated genomic DNA from whole blood of healthy Japanese individuals and sequenced the promoter regions of the MDM2, p53, and p16INK4a genes. We identified polymorphisms comprising 3 nucleotide substitutions at exon 1 and intron 1 regions of the MDM2 gene and 1 nucleotide insertion at a poly(C) nucleotide position in the p53 gene. The Japanese individuals also exhibited p16INK4a polymorphisms at several positions, including position -191. Reporter gene analysis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Murine double minute 2; Polymorphism; P16INK4a; P53; Transcription. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400004 |
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| Cardoso,Greice Lemos; Takanashi,Silvania Yukiko Lins; Guerreiro,João Farias. |
| The most common hemoglobinopathies, viz, hemoglobins S and C, and α-and β-thalassemias, were investigated through the molecular screening of 116 subjects from the community of Saracura, comprising fugitive African slaves from farms of the municipality of Santarém, in the west of Pará State, Brazilian Amazon. The observed frequency of the HBB*S gene (0.9%) was significantly lower than that encountered in other Afro-derived communities in the region. Concomitantly, the absence of the HBB*C allele has been reported for most of the Afro-Amazonian communities thus far studied. As remnant populations of quilombos are generally small, the heterogeneous distribution of HBB*S and HBB*C alleles among them is probably due to genetic drift and/or founder effect. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sickle cell disease; Thalassemias; Afro-Amazonian. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400002 |
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| Guerra,Marcelo. |
| Abstract Agmatoploidy is a type of chromosome rearrangement that involves the fragmentation of an entire chromosome complement, generating a diploid with double its original chromosome number. Agmatoploidy and other related karyotype changes, such as symploidy (the opposite change, promoted by chromosome fusion), partial agmatoploidy, polyagmatoploidy, etc., are restricted to species with holokinetic chromosomes and are assumed to play an important role in their karyotype evolution. However, a critical review of the literature shows that examples of chromosome number doubling by agmatoploidy are rare and not clearly demonstrated, while partial agmatoploidy and partial symploidy seem to be the same as ascending and descending disploidy, respectively. It is... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agmatoploidy; Symploidy; Holocentrics; Holokinetic chromosomes; Chromosome evolution. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400492 |
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| Koppe,Tiago; Doneda,Divair; Siebert,Marina; Paskulin,Livia; Camargo,Matheus; Tirelli,Kristiane Michelin; Vairo,Filippo; Daudt,Liane; Schwartz,Ida Vanessa D.. |
| Abstract The clinical utility of serum ferritin as a biomarker of disease severity and prognosis in Gaucher disease (GD) is still debated. Here, we aimed to evaluate ferritin and its relation to clinicolaboratory parameters of GD patients seen at the Reference Center for Gaucher Disease of Rio Grande do Sul, Brazil, so as to gather evidence on the utility of ferritin as a biomarker of this condition. A retrospective chart review was performed collecting pre-and posttreatment data from GD patients. Eighteen patients with ferritin levels available before and after treatment were included in the study. Nine of these participants were males, and seventeen had type I GD. All patients were given either enzyme replacement (n = 16) or substrate reduction therapy... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ferritin; Biomarkers; Gaucher disease; Iron metabolism. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100030 |
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| Diniz-Filho,José Alexandre F.; Soares,Thannya N.; Lima,Jacqueline S.; Dobrovolski,Ricardo; Landeiro,Victor Lemes; Telles,Mariana Pires de Campos; Rangel,Thiago F.; Bini,Luis Mauricio. |
| The comparison of genetic divergence or genetic distances, estimated by pairwise F ST and related statistics, with geographical distances by Mantel test is one of the most popular approaches to evaluate spatial processes driving population structure. There have been, however, recent criticisms and discussions on the statistical performance of the Mantel test. Simultaneously, alternative frameworks for data analyses are being proposed. Here, we review the Mantel test and its variations, including Mantel correlograms and partial correlations and regressions. For illustrative purposes, we studied spatial genetic divergence among 25 populations of Dipteryx alata ("Baru"), a tree species endemic to the Cerrado, the Brazilian savannas, based on 8 microsatellite... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: "Baru" tree; Genetic distances; Geographical genetics; Partial correlation; Partial regression. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400002 |
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| Pignata,Maria Izabel Barnez; Stort,Antonio Carlos; Malaspina,Osmar. |
| The lengths of the mouthparts of bees, the glossa, paraglossa, stipes, galea, labial palpus, maxillary palpus, cardo, lorum, mentum and prementum, were studied in Caucasian and Africanized bees and in their F1 descendants. Only the lengths of the paraglossa, stipe, galea, mentum, prementum and maxillary palpus differed significantly between these two bee types. These six variables were studied in the F1 descendants of two types of crosses, i.e., Caucasian queens x Africanized males (cross 1) and Africanized queens x Caucasian males (cross 2). Multidimensional analyses were also performed and the generalized Mahalanobis distances (D2) between the F1 descendants and the parental lines were determined. There was an apparent dominance of Africanized bees in... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400010 |
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| Granada,Camille E; Strochein,Marcos; Vargas,Luciano K; Bruxel,Manuela; Sá,Enilson Luiz Saccol de; Passaglia,Luciane M.P. |
| This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically analyzed by the BOX-PCR technique, which showed a high genetic diversity among the rhizobia studied. The isolates were also characterized based on their production of indolic compounds and siderophores, as well as on their tolerance to salinity. Fifteen of the 33 rhizobia analyzed were able to produce indolic compounds, whereas 13 produced siderophores. All the tested rhizobia were sensitive to high salinity, although some were able to grow in solutions of up to 2%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rhizobia; Nodulation; Nitrogen; Specificity. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300012 |
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| Mahasneh,Amjad; Jubaili,Amal; El Bateiha,Ahmed; Al-Ghazo,Mohammad; Matalka,Ismail; Malkawi,Mousa. |
| The arylamine N-acetyltransferase 2 (NAT2) enzymes detoxify a wide range of naturally occurring xenobiotics including carcinogens and drugs. Point mutations in the NAT2 gene result in the variant alleles M1 (NAT2 *5A), M2 (NAT2*6A), M3 (NAT2*7) and M4 (NAT2 *14A) from the wild-type WT (NAT2 *4) allele. The current study was aimed at screening genetic polymorphisms of NAT2 gene in 49 lung cancer patients, 54 colorectal cancer patients and 99 cancer-free controls, using PCR-RFLP. There were significant differences in allele frequencies between lung cancer patients and controls in the WT, M2 and M3 alleles (p < 0.05). However, only M2 and M3 allele frequencies were different between colorectal cancer patients and controls (p < 0.05). There was a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: NAT2; Lung cancer; Colorectal cancer; Polymorphisms. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500003 |
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| Baracho,Marta S.; Baracho,Ivanhoé R.. |
| Mutations related to gene methG1 of Aspergillus nidulans were analyzed, in order to study a mathematical model for the determination of the mutation rate per nucleus per generation, in filamentous fungi. A replica plating technique was used to inoculate, in a single operation, 26 colonies of the strain, into Petri dishes containing culture medium, and the nine central colonies were analyzed for size and number of conidia in each colony. Using this technique, several central colonies were analyzed with regard to the appearance of mutation, and the number and type of reversions were determined for each colony. The frequencies obtained for each reversion were analyzed, in order to verify if their distribution was in accordance with that of Greenwood and Yule.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus nidulans; Mutation rate; Spontaneous mutation; Fungi; Filamentous fungi. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100014 |
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| Registros recuperados: 2.463 | |
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