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Occurrence of mycootoxins in alfalfa (Medicago sativa L.), sorghum [Sorghum bicolor (L.) Moench], and grass (Cenchrus ciliaris L.) retailed in the state of Nuevo León, México Agrociencia
Huerta-Treviño,Alejandra; Dávila-Aviña,Jorge E.; Sánchez,Eduardo; Heredia,Norma; García,Santos.
Abstract Mycotoxins are secondary metabolites produced by specific filamentous fungi that are common contaminants of agricultural commodities. These compounds are toxic to humans and animals, and they are a health problem worldwide. Mycotoxin-containing feeds can cause serious diseases in farm animals and substantial economic losses. Therefore, the objective of this study was to determine the occurrence and levels of six mycotoxins in three types of forage at retail in the state of Nuevo León, México. The hypothesis was that all types of forages were contaminated with at least one of the six mycotoxins analyzed. One hundred and twenty samples of alfalfa (Medicago sativa L.), sorghum [Sorghum bicolor (L.) Moench], and grass (Cenchrus ciliaris L.) used for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycotoxins; Forages; Aflatoxin; Fumonisin; Ochratoxin.
Ano: 2016 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952016000700825
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Nutritional changes in powdered red pepper upon in vitro infection of Aspergillus flavus BJM
Tripathi,Smita; Mishra,H.N..
Quantitative losses in various biochemical constituents like capsaicin, carotenes, ascorbic acid, polyphenols, mineral matter, sugars (soluble and insoluble), protein and fat were estimated after the successful growth of Aspergillus flavus for 30 days on powdered red pepper. The fungal biomass was measured by ergosterol content and Aflatoxin B1 by HPLC. Amongst the various nutritional constituents evaluated for nutritional losses and changes the highest nutritional loss was reported in total carotenoids (88.55%) followed by total sugars (85.5%). The protein content of the infected sample increased from 18.01% to 23%. The nutritional profile of chilli powder (Capsicum annum var. sannam L.) shows highest share of total soluble sugars (32.89%) and fiber...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus flavus; Chili powder; Nutritional changes; Aflatoxin.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100024
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Sampling plan for the analysis of aflatoxin in peanuts and corn: an update BJM
Fonseca,Homero.
The aim of this paper was to update the sampling plan for analysis of mycotoxins in grains, formerly published by the author. The proposed alterations were based on the acquired experience on its application and on FAO recommendations. This update restricts the scope of the former plan and establishes a sampling plan for analysis of aflatoxin in peanuts and corn, by means of modified formulas, the minimum number of sacks or points (when in bulk) from which incremental samples should be drawn to make a bulk sample. Fractional exponents (square roots) of the formulas proportionally decrease the number of sacks/points to be sampled as the lot size increases. Operating Characteristic (OC) curves developed for in-shell and shelled peanuts and corn as well as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sampling; Peanuts; Corn; Aflatoxin; Analysis.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200001
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Production of aflatoxins by Aspergillus flavus and of fumonisins by Fusarium species isolated from Brazilian sorghum BJM
Silva,Josefa B. da; Dilkin,Paulo; Fonseca,Homero; Corrêa,Benedito.
Fifty-nine Aspergillus flavus and 35 Fusarium verticillioides strains, isolated from freshly harvested (10) and stored (130) Brazilian sorghum samples, were tested regarding their ability to produce aflatoxins (coconut milk agar) and fumonisins (rice culture), respectively. Aflatoxins B1 and B2 were detected by TLC, and fumonisins B1 and B2 were analyzed by HPLC. Thirty-eight (64.4%) A. flavus strains produced detectable levels of aflatoxins at concentrations ranging from 12.00 to 3282.50 µg/kg (AFB1 + AFB2), while thirty two (91%) F. verticillioides strains produced FB1 at concentrations ranging from 0.12 to 5.38 µg/g. Two F. proliferatum strains produced low fumonisin levels. The toxigenic potential of A. flavus (64.4%) and F. verticillioides (91.5%)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxigenicity; Aspergillus flavus; Fusarium verticillioides; Mycotoxins; Sorghum; Aflatoxin; Fumonisin.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200002
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Distribution of aflatoxins in contaminated corn fractions segregated by size BJM
Piedade,Fabiana S.; Fonseca,Homero; Glória,Eduardo M.; Calori-Domingues,Antonia; Piedade,Sônia M.S.; Barbin,Décio.
The efficiency of segregation, by particle size, in reducing the aflatoxin content of corn lots under the Brazilian conditions was studied. The aflatoxin content, in the corn fractions obtained after sieving thirty contaminated samples, with approximately 3.0 kg each, was determined. The samples were passed through 4.5 mm round-hole sieves and the fractions with particles ³4.5 mm (fraction A) and <4.5 mm (fraction B) and then analyzed for aflatoxins by TLC. The results showed that the distribution of the aflatoxins among the fractions A and B varied from sample to sample. However, the fraction B presented higher content (6 to 1,422 µg/kg) of aflatoxins (P<5%), than samples of fraction A (4 to 389 µg/kg). In spite of that the segregation of this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Corn; Contamination distribution; Segregation; Fractions.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100002
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Molecular characterisation of Aspergillus flavus isolates from peanut fields in India using AFLP BJM
Singh,Diwakar; Radhakrishnan,T.; Kumar,Vinod; Bagwan,N.B.; Basu,M.S.; Dobaria,J.R.; Mishra,Gyan P.; Chanda,S.V..
Aflatoxin contamination of peanut, due to infection by Aspergillus flavus, is a major problem of rain-fed agriculture in India. In the present study, molecular characterisation of 187 Aspergillus flavus isolates, which were sampled from the peanut fields of Gujarat state in India, was performed using AFLP markers. On a pooled cluster analysis, the markers could successfully discriminate among the ‘A’, ‘B’ and ‘G’ group A. flavus isolates. PCoA analysis also showed equivalent results to the cluster analysis. Most of the isolates from one district could be clustered together, which indicated genetic similarity among the isolates. Further, a lot of genetic variability was observed within a district and within a group. The results of AMOVA test revealed that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; AMOVA; ELISA; Genetic diversity; Groundnut; PCA.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300673
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Inhibitory activity of compounds isolated from Polymnia sonchifolia on aflatoxin production by Aspergillus flavus BJM
Pak,Adriana; Gonçalez,Edlayne; Felicio,Joana D'arc; Pinto,Marina Mori; Rossi,Maria Helena; Simoni,Isabela Cristina; Lopes,Márcia Nasser.
Polymnia sonchifolia, commonly known as ";yacon";, was originally cultivated at Andes moutains in South America. Recently, the specie attracted worldwide attention because of its wide range of uses, for example in the control of diabetes melitus, besides the antifungal and pesticidal compounds were found in the leaves. This study describes the identification of two flavonoids: 3', 5, 7 trihydroxy-3, 4'-dimethoxyflavone (compound 1) and 3', 4', 5- trihydroxy-7-methoxy flavanone (compound 2) and two sesquiterpenes lactones: enhydrin (compound 3) and a mixture of enhydrin and uvedalin (compound 4) isolated from Polymnia sonchifolia leaves and their effects on the aflatoxin production by Aspergillus flavus. The identification of the compounds were achieved by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polymnia sonchifolia; Flavonoids; Melampolides; Aflatoxin; Aspergillus flavus.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200018
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Toxigenic fungi in beans (Phaseolus vulgaris L.) classes black and color cultivated in the State of Santa Catarina, Brazil BJM
Costa,Léa Luzia Freitas; Scussel,Vildes Maria.
Toxigenic fungi were studied in beans (Phaseolus vulgaris L.) of Classes black and color, cultivated in different regions of the State of Santa Catarina, south region of Brazil. The mean counts of filamentous fungi were 2.8 x 103 and 6.7 x 103 CFU/g for beans Classes black and color, respectively. Penicillium spp., Aspergillus spp. and Phoma spp. were the most frequent genera isolated, followed by Ryzopus spp., Alternaria spp., Helminthosporium spp., Cladosporium spp., Botrytis spp., Fusarium spp., Trichoderma spp., Curvularia spp. and Dreschelera spp. Among beans Class black, 24.6% of the Aspergillus strains produced mycotoxins: 13.1% produced aflatoxins (AFs); 11.5% produced ochratoxin A (OTA) and 28.9% of Penicillium produced citrinin (CTR). On the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beans; Phaseolus; Toxigenic fungi; Aflatoxin; Ochratoxin A; Citrinin.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200008
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The application of PCR in the detection of mycotoxigenic fungi in foods BJM
Konietzny,Ursula; Greiner,Ralf.
It is estimated that 25 to 50% of the crops harvested worldwide are contaminated with mycotoxins. Because of the toxic and carcinogenic potential of mycotoxins, there is an urgent need to develop detection methods that are rapid and highly specific. The highly advanced physico-chemical methods for the analysis of mycotoxins in use, have the disadvantage that highly sophisticated clean-up and/or derivatization procedures must be applied. An alternative could be the detection of the mycotoxigenic moulds themselves, especially as molecular techniques have been introduced recently as powerful tools for detecting and identifying fungi. PCR methods for the detection of aflatoxigenic Aspergilli, patulin-producing Penicillum and trichothecene- as well as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Fumonisin; Patulin; Polymerase chain reaction; Trichothecene.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400001
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The potential effects of Zataria multiflora Boiss essential oil on growth, aflatoxin production and transcription of aflatoxin biosynthesis pathway genes of toxigenic Aspergillus parasiticus BJM
Yahyaraeyat,R.; Khosravi,A.R.; Shahbazzadeh,D.; Khalaj,V..
This study aims at evaluating the effects of Zataria multiflora (Z. multiflora) essential oil (EO) on growth, aflatoxin production and transcription of aflatoxin biosynthesis pathway genes. Total RNAs of Aspergillus parasiticus (A.parasiticus) ATCC56775 grown in yeast extract sucrose (YES) broth medium treated with Z. multiflora EO were subjected to reverse transcription-polymerase chain reaction (RT-PCR). Specific primers of nor-1, ver-1, omt-A and aflR genes were used. In parallel mycelial dry weight of samples were measured and all the media were assayed by high-pressure liquid chromatography (HPLC) for aflatoxinB1 (AFB1), aflatoxinB2 (AFB2), aflatoxinG1 (AFG1), aflatoxinG2 (AFG2) and aflatoxin total (AFTotal) production. The results showed that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zataria multiflora; Reverse transcription-polymerase chain reaction; Aflatoxin; Aspergillus parasiticus.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200045
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Immobilized Saccharomyces cerevisiae as a potential aflatoxin decontaminating agent in pistachio nuts BJM
Rahaie,S.; Emam-Djomeh,Z.; Razavi,S. H.; Mazaheri,M..
In this study, we investigated the binding ability of Saccharomayces cerevisiae to aflatoxin in pistachio nuts. The obtained results indicate that S. cerevisiae has an aflatoxin surface binding ability of 40% and 70% (with initial aflatoxin concentrations of 10 and 20 ppb) in the exponential phase. Acid treatments increase this ability to approximately 60% and 73% for the two concentrations of aflatoxin, respectively. Heat treatments also enhance surface binding to 55% and 75%, respectively. Binding appears to be a physical phenomenon that saturates within the first 2-3 hours of the process. The obtained results indicate that yeast immobilization for toxin reduction on aflatoxin-contaminated pistachios had no effect on qualitative characteristics, such as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Pistachio nut; Saccharomayces cerevisiae; Surface binding.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100014
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Risk assessment of the occurrence of aflatoxin and fungi in peanuts and cashew nuts BJPS
Kujbida,Paula; Maia,Patrícia Penido; Araújo,Ariadne Naama de; Mendes,Leonardo Daniel; Oliveira,Mariana Lepri de; Silva-Rocha,Walicyranison Plinio; Brito,George Queiroz de; Chaves,Guilherme Maranhão; Martins,Isarita.
In the present study, the occurrence of fungi and aflatoxins (AFs) in peanut and cashew nut samples was investigated. Mycological analysis revealed the presence of fungi in 58.8% of samples, and assessment of AFs by chromatographic methods revealed that 52.9% were contaminated by AFs. AFB1 was the principal component in all AF-contaminated samples, with a mean level of 14.0, and 1.08 µg/kg in peanut and cashew nut, respectively. Eleven samples (32.4%) exceeded the total AF maximum level (4 μg/kg) and 8 samples (23.5%) exceeded the AFB1 (2 μg/kg) established by the European Commission. Our findings suggest that the incidence of AFs emphasizes the need for regular monitoring and a more stringent food safety system to control AFs at the lowest possible levels...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Peanut; Cashew nut; Fungi; Risk assessment.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100502
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Effect of drying methods on long term storage of hazelnut Ciênc. Tecnol. Aliment.
TURAN,Ali; KARAOSMANOĞLU,Hasan.
Abstract This research was conducted to determine the chemical traits of Ordu Levant hazelnuts that were dried with sun–drying [on concrete ground (CG), grass ground (GG)], or in drying machine (DM) during 24 months of storage (2014-2016) at 20-25 °C and 70-90% relative humidity. Drying process continued 39 h for CG and GG, and 23 h for DM. Sun–drying were performed in similar sunshine and environmental conditions. Nut quality traits in protein, lipid, moisture contents, water activity, free fatty acids (FFA), peroxide value (PV), rancimat value (RV), and amount of aflatoxin were investigated during storage. The lowest FFA (0.35%, oleic acid) was recorded for GG, and the lowest PV (0.35 meqO2 kg–1 ) was recorded for CG. In addition, DM method contained...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Concrete ground; Drying machine; Free fatty acid; Peroxide value; Rancimat value.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000600406
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Comparison of methods by TLC and HPTLC for determination of aflatoxin M1 in milk and B1 in eggs Ciênc. Tecnol. Aliment.
Scussel,V. M..
Milk and egg matrixes were assayed for aflatoxin M1 (AFM1) and B1 (AFB1) respectively, by AOAC official and modified methods with detection and quantification by thin layer chromatography (TLC) and high performance thin layer chromatography (HPTLC). The modified methods: Blanc followed by Romer, showed to be most appropriate for AFM1 analysis in milk. Both methods reduced emulsion formation, produced cleaner extracts, no streaking spots, precision and accuracy improved, especially when quantification was performed by HPTLC. The use of ternary mixture in the Blanc Method was advantageous as the solvent could extract AFM1 directly from the first stage (extraction), leaving other compounds in the binary mixture layer, avoiding emulsion formation, thus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; AFM1; AFB1; TLC; HPTLC; Milk; Egg.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612003000400009
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Mycoflora and aflatoxigenic species in derivatives of milled rice Ciênc. Tecnol. Aliment.
LIMA,Carlos A. P.; ORSI,Reinaldo B.; DILKIN,Paulo; CORRÊA,Benedito.
Thirty samples of rough rice stored for 6, 12 and 24 months in government authorized warehouses of the state of Rio Grande do Sul, Brazil, were simultaneously collected. After milling of the product, 90 samples (30 of polished rice, 30 of rice bran and 30 of rice hull) were evaluated for their mycoflora, aflatoxigenic species and aflatoxin contamination. The following fungi, listed in decreasing order of frequency, were isolated on Potato-Dextrose Agar: Aspergillus spp., Nigrospora spp., Penicillium spp.; Fusarium spp.; Mucor spp.; Cladosporium spp.; Trichosporon spp. and non-sporulated fungi. The degree of fungal contamination (colony forming units per gram of product) was lowest in polished rice, increasing progressively in samples of rice bran and rice...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Aspergillus flavus; Mycoflora; Milled rice; Stored rice.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612000000100008
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Distribution of aflatoxin contamination in maize samples Ciênc. Tecnol. Aliment.
Gloria,Eduardo Micotti da; Ciacco,César Francisco; Lopes Filho,José Francisco; Ericsson,Cynthia; Zocchi,Silvio Sandoval.
The distribution of the aflatoxin contamination was studied among four maize fractions, separated according to Brazilian grading rules for maize. The fraction that contained fermented, moldy, heated and sprouted grains normally had the highest levels of aflatoxin. However, the fraction contribution to the whole sample contamination level took into account the contamination fraction level and its weight to the whole sample. Considering this, the fraction that contained insect damaged, hollow, up to ¼ fermented and grains damaged by other causes was normally the fraction responsible for the total contamination level in the samples. Nevertheless, the fraction contributions were variable from sample to sample. Therefore, in conclusion, it was not possible to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Contamination; Distribution; Grain type; Maize.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612004000100014
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A model for implementation of HACCP system for prevention and control of mycotoxins during the production of red dried chili pepper Ciênc. Tecnol. Aliment.
OZTURKOGLU-BUDAK,Sebnem.
Abstract Hazard analysis and critical control points (HACCP) is a process control system describes the potential hazards during food production flow chart. In contrast to the conventional way of ensuring product safety by end-product testing, HACCP establishes control systems, focuses mainly on preventive measures. Quality of red dried chili pepper (RDCP) should be assured by taking special care from harvesting of the peppers to retailing of the packaged product. RDCP is susceptible to fungi growth and mycotoxin contamination depending on the atmospheric and processing conditions such as humidity, temperature, storage and drying. Mycotoxins, are toxic metabolites to human health and strict control measures are required for preventing their occurrence in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dry chili pepper; HACCP; Aflatoxin.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000500024
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Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach Ciência Rural
Silva,Janaina Lara da; Aparecido,Christiane Ceriani; Hansen,Daiane; Pereira,Tacila Alves Muniz; Felicio,Joana D'arc; Gonçalez,Edlayne.
Some species of filamentous fungi that infest agricultural commodities are able to produce mycotoxins, contaminating feed and animal products. The aim of this research was to identify the mycoflora present in the feed and forage for dairy goat and to isolate and characterize the Aspergillus flavusand A. parasiticusstrains based on a morphological and molecular characterization and mycotoxigenic ability. The goat dairy diets were collected monthly from 11 goat milk farms, totaling 129 and 106 samples of concentrate and forage, respectively. For the isolation of the mycobiota the surface plating method was used. Aspergillus, Penicillium,and Fusariumwere the main fungi producing mycotoxins isolated. The morphological and molecular characterization and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxigenic fungi; Aflatoxin; Cyclopiazonic acid; Feed; Molecular characterization..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801466
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Amendoim sem aflatoxina nos cerrados. Infoteca-e
FIGUEREDO, S. F.; AZEVEDO, J. A. de.
Publicado também em: Quaero, 17 dez. 2009; Agronline, 17 dez. 2009; Cultivarnet, 18 dez. 2009; Sociedade Sustentável, 18 dez. 2009.
Tipo: Artigo na mídia Palavras-chave: Peanut; Aflatoxin; Amendoim; Cerrado; Aflatoxina; Arachis.
Ano: 2009 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/658750
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Association of the severity of tongue lesions in broiler chickens with the quality of corn in feed PAB
Vaccari,Isabel Cristina Mores; Duarte,Vinicius; Rodrigues,Denise Russi; Santos,Fabiana Ramos dos; Minafra,Cibele Silva.
Abstract: The objective of this work was to determine the correlation of the physical attributes of corn and the mycotoxins present in its grains with the severity of tongue lesions in broiler breeder roosters. The occurrence of the aflatoxin, vomitoxin, zearalenone, T-2 toxin, and fumonisin mycotoxins was detected in the corn grains used in the feed formulation for breeder chickens. The percentages of moisture and broken grains were positively correlated (Pearson’s correlation) with the presence of aflatoxin. Milder lesions (grade 1) are positively correlated with aflatoxin and negatively correlated with fumonisin, and the most severe lesions (grade 3) are positively correlated with vomitoxin, fumonisin, and T-2 toxin.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Aviculture; Fumonisin; Mycotoxin; T-2 toxin; Vomitoxin..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001000942
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