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Registros recuperados: 35
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Incidencia de nemátodos entomopatógenos en áreas cañeras de Veracruz y su interacción con el barrenador de la caña de azúcar Diatraea saccharalis. Colegio de Postgraduados
Grifaldo Alcántara, Pedro Fabián.
Nematodos entomopatógenos fueron colectados con el método de insectos cebo usando larvas de Galleria mellonella, en áreas de caña de azúcar en el municipio de Paso del Macho, Veracruz, México. Se obtuvieron tres aislamientos (MTB-P7-P9), los cuales fueron identificados como la misma especie por estudios morfológicos, morfométricos de las diferentes etapas de desarrollo, y por la caracterización filogenética y secuencias parciales de la región ITS del ADNr. El nematodo se encuentra taxonómicamente cercano a Steinernema riobrave y esta filogenéticamente relacionado con el grupo IV (bicornutum-certaophorum-riobrave), grupo caracterizado por la presencia de proyecciones “tipo cuernos” en la región de la cabeza de los juveniles infectivos (JI). Steinernema...
Palavras-chave: Bioensayos; Descripción; ITS-ADNr; Morfología; Morfométria; Nematodos entomopatógenos; Taxonomía; Bioassays; Nematodes; Entomopathogenic; Maestría; Entomología y Acarología.
Ano: 2011 URL: http://hdl.handle.net/10521/384
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Incidencia de nemátodos entomopatógenos en áreas cañeras de Veracruz y su interacción con el barrenador de la caña de azúcar Diatraea saccharalis. Colegio de Postgraduados
Grifaldo Alcántara, Pedro Fabián.
Nematodos entomopatógenos fueron colectados con el método de insectos cebo usando larvas de Galleria mellonella, en áreas de caña de azúcar en el municipio de Paso del Macho, Veracruz, México. Se obtuvieron tres aislamientos (MTB-P7-P9), los cuales fueron identificados como la misma especie por estudios morfológicos, morfométricos de las diferentes etapas de desarrollo, y por la caracterización filogenética y secuencias parciales de la región ITS del ADNr. El nematodo se encuentra taxonómicamente cercano a Steinernema riobrave y esta filogenéticamente relacionado con el grupo IV (bicornutum-certaophorum-riobrave), grupo caracterizado por la presencia de proyecciones “tipo cuernos” en la región de la cabeza de los juveniles infectivos (JI). Steinernema...
Palavras-chave: Bioensayos; Descripción; ITS-ADNr; Morfología; Morfométria; Nematodos entomopatógenos; Taxonomía; Bioassays; Nematodes; Entomopathogenic; Maestría; Entomología y Acarología.
Ano: 2011 URL: http://hdl.handle.net/10521/384
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Bioensaios de toxicidade genética com Tradescantia. Infoteca-e
RODRIGUES, G. S..
Resumo: Dentre a variedade de bioensaios disponíveis para estudos de detecção e monitoração dos efeitos tóxicos genéticos de substâncias e ambientes contaminados, os bioensaios que empregam a planta Tradescantia destacam-se pela sensibilidade e facilidade de realização de estudos de curto prazo in situ. Este trabalho apresenta uma revisão extensiva dos estudos de mutagênese ambiental empregando Tradescantia como bioindicador, e é complementado com resultados experimentais e indicações metodológicas.
Tipo: Documentos (INFOTECA-E) Palavras-chave: Bioensaio; Toxidez; Mutação; Agrotóxico; Tradescantia; Bioassays; Mutagenesis; Genotoxicity; Pesticides.
Ano: 1999 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1051547
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Bioensaios de toxicidade genética com plantas superiores Tradescantia (MCN e SHM), milho e soja. Infoteca-e
RODRIGUES, G. S..
A avaliação da toxicidade genética presente em certas situações ambientais e de compostos selecionados é uma área de crescente interesse em ecotoxicologia. Neste trabalho, são descritos quatro bioensaios vegetais de interesse para avaliação de genotoxicidade in situ em ambientes agrícolas. O ensaio do micronúcleo em célula mãe do pólen de Tradescantia (Trad-MCN) é um teste de clastogênese extremamente sensível, de exposição curta (desde apenas 6 horas) e avaliação simples. O ensaio do pelo estaminal (Trad-SHM) B um teste de mutação pontual, no qual se observa alteração de cor nas células do estame. A avaliação é simples, mas laboriosa. O bioensaio do pólen ceroso em milho tem como principal vantagem a sua potência estatística, já que, literalmente,...
Tipo: Folhetos Palavras-chave: Toxicidade; Ecotoxicologia; Bioensaio; Monitoramento ambiental; Pesticide; Soybean; Agrotóxico; Milho; Soja; Mutação; Toxidez; Genética; Environmental impact; Mutagenesis; Tradescantia; Corn; Bioassays.
Ano: 1999 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/964416
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Methods for microbial biomass, community structure and metabolic activities on surfaces. ArchiMer
White, D.
Microbes in nature are often concentrated on surfaces where they exist in microcolonies of mixed composition. The assay of these mixed microbial consortia by utilizing biochemical components eliminates the problems associated with cultural selectivity of viable counts or the requirement for quantitative release from surfaces required in some microscopic techniques. The assay of cellular components restricted to subsets of the microbial community gives insights into the community structure of the biofilm whereas assay of cellular components common to all cells can be used for biomass estimations. Formation of endogenous storage materials can be utilized as measures of nutritional status and rates of formation or turnover of specific "signatures" from...
Tipo: Text Palavras-chave: Bacteria; Fouling; Bioassays; Metabolism; Surfaces; Bacteria collecting devices; Culture media; Microbiological analysis.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-963.pdf
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Innovative rapid solid-phase immunobead assay for the detection of okadaic acid and related DSP toxins in shellfish ArchiMer
Park, D; Fremy, J; Marcaillou, L; Gamboa, P; Gleizes, E; Masselin, P; Goldsmith, C.
The solid-phase immunobead assay (Ciguatect super(TM)) has been optimized to detect toxins associated with diarrheic shellfish poisoning (DSP). The Ciguatect super(TM) test kit utilizes a membrane to bind the toxins and a monoclonal antibody-latex bead solution as the determination step. The presence or absence of okadaic acid (OA) and related toxins is determined by binding the toxins to a membrane attached to a plastic strip and exposing the toxins ladened membrane to a monoclonal antibody-colored latex bead complex which has a high specificity for OA and related polyether compounds. The intensity of the color on the membrane denotes the presence of the toxins. A rapid extraction method (REM super(TM)) has been developed to allow for confirmation of...
Tipo: Text Palavras-chave: Dinophysis; Bivalvia; Algae; Marine molluscs; Immunology; Bioassays; Disease detection; Biological poisons; Shellfish.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1604.pdf
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Integrated monitoring of chemicals and their effects on four sentinel species, Limanda limanda, Platichthys flesus, Nucella lapillus and Mytilus sp, in Seine Bay: A key step towards applying biological effects to monitoring ArchiMer
Burgeot, Thierry; Akcha, Farida; Menard, Dominique; Robinson, Craig; Loizeau, Veronique; Brach-papa, Christophe; Martinez-gomez, Concepcion; Le Goff, Jeremie; Budzinski, Helene; Le Menach, Karine; Cachot, Jerome; Minier, Christophe; Broeg, Katja; Hylland, Ketil.
The International workshop on Integrated Assessment of CONtaminants impacts on the North sea (ICON) provided a framework to validate the application of chemical and biological interpretation thresholds (BACs and EACs) in the Seine Bay in France. Bioassays (oyster larval anomalies, Corophium arenarium toxicity assay and DR Calux) for sediment and biomarkers: ethoxyresorufin-O-deethylase (EROD) activity, acetylcholinesterase (AChE) activity, lysosomal membrane stability (LMS), DNA strand breaks using the comet assay, DNA adducts, micronucleus (MN), PAH metabolites, intersex and fish external pathologies were analyzed in four marine sentinel species (Platichthys flesus, Limanda limanda, Mytilus sp. and Nucella lapilus). Polycyclic aromatic hydrocarbons...
Tipo: Text Palavras-chave: Biomarkers; Bioassays; Chemical contamination; Integrated monitoring in chemistry and biology; Local study; Large geographical scale.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00357/46789/46655.pdf
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Requirements for screening and confirmatory methods for the detection and quantification of marine biotoxins in end-product and official control ArchiMer
Hess, Philipp.
An overview is given of the biological origin of phycotoxins, as well as their chemical characteristics. Major poisoning types are described and examples of poisoning events are given to illustrate the importance of the phenomenon to both shellfish consumers and the shellfish producing industry. The characteristics of phycotoxins as natural products, the lack of predictability of their occurrence, economic drivers and the freshness of shellfish consumed in many countries result in a number of requirements for methods to be used in the efficient detection of these compounds. Subsequently, the performance of mouse bioassays and mass spectrometry as detection tools are compared for examples from Irish and French monitoring programmes to assess the usefulness...
Tipo: Text Palavras-chave: Bioanalytical methods; Bioassays; Mass spectrometry; Liquid chromatography; Biological samples.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00006/11767/8989.pdf
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Viral contamination and depuration of oysters under field conditions ArchiMer
Boher, S; Piclet, Guy; Beril-stien, S; Masson, Daniel; Delonce, R; Biziagos, E; Schwartzbrod, L.
Shellfish consumption is clearly associated with the transmission of enteric viruses. The channel of transmission of enteric viruses to human beings could be decreased particularly if the systems of depuration of shellfish were optimized, standardized and if their viral efficiency was controlled. The purpose of this work was to study the contamination of oysters and to assess the efficiency of several depuration processes. Our study have demonstrated that in some cases, 50% of oysters samples contain enteric viruses or H.A.V. antigen and the quantities of viruses range from 17 to 246 FF for the rotaviruses and from 124 to 200 for the enteroviruses per 100 g of tissue. According to our results, the exclusive use of a bacteriological criterium to determine...
Tipo: Text Palavras-chave: Rotavirus; Hepatitis A virus; Enterovirus; Crassostrea gigas; Bivalvia; Seafood; Bioassays; Cell culture; Viruses; Self purification; Viral diseases; Shellfish.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1615.pdf
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Phytoplankton phosphorus limitation in a North Atlantic coastal ecosystem not predicted by nutrient load ArchiMer
Trommer, Gabriele; Leynaert, Aude; Klein, Cecile; Naegelen, Aurore; Beker, Beatriz.
Phytoplankton nutrient limitation patterns were investigated in an anthropogenically influenced coastal ecosystem with a high nutrient load. Weekly nutrient limitation bioassays, and water chemistry and stoichiometry measurements were performed in the Bay of Brest, France, from February to July 2011. Each limitation bioassay included phosphorus, nitrogen, silicate and all possible combined nutrient additions, and lasted over 72 h. Results showed that the phytoplankton community experienced a general P limitation from March to July. N limitation alone was observed only during 1 week in early March. Subsequently, all limitation bioassays revealed primary P limitation, indicated by significantly increased growth rates in all samples containing P additions....
Tipo: Text Palavras-chave: Phytoplankton; Stoichiometry; Bioassays; Nutrient limitation; Coastal ecosystem.
Ano: 2013 URL: https://archimer.ifremer.fr/doc/00368/47947/48023.pdf
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The toxicity of composted sediments from Mediterranean ports evaluated by several bioassays ArchiMer
Mamindy-pajany, Yannick; Hamer, Bojan; Romeo, Michele; Geret, Florence; Galgani, Francois; Durmisi, Emina; Hurel, Charlotte; Marmier, Nicolas.
This work investigates the ecotoxicological evaluation of contaminated dredged sediments from French Mediterranean navy harbour (A), commercial port (B) and two composite specimens (C) and (D) coming from the mixture of A and B with other port sediments. The toxicity of elutriates from these sediments is estimated using embryo-toxicity test. Microtox (R) solid phase test, LuminoTox, phytotoxicity tests and genotoxicity test. Bioassay responses are not clearly correlated with chemical contamination in the whole sediment and vary as a function of tested organisms. The highest contaminated samples (A and C) are almost always more toxic than the less contaminated samples (B and D). Among composite sediments, the mixture effect with other sediments is not...
Tipo: Text Palavras-chave: Bioassays; Dredged port sediments; Phytotoxicity; Microtox (R) solid phase; Embryo-toxicity test; Genotoxicity.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00031/14262/12284.pdf
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Mixtures of Chemical Pollutants at European Legislation Safety Concentrations: How Safe Are They? ArchiMer
Carvalho, Raquel N.; Arukwe, Augustine; Ait-aissa, Selim; Bado-nilles, Anne; Balzamo, Stefania; Baun, Anders; Belkin, Shimshon; Blaha, Ludek; Brion, Francois; Conti, Daniela; Creusot, Nicolas; Essig, Yona; Ferrero, Valentina E. V.; Flander-putrle, Vesna; Furhacker, Maria; Grillari-voglauer, Regina; Hogstrand, Christer; Jonas, Adam; Kharlyngdoh, Joubert B.; Loos, Robert; Lundebye, Anne-katrine; Modig, Carina; Olsson, Per-erik; Pillai, Smitha; Polak, Natasa; Potalivo, Monica; Sanchez, Wilfried; Schifferli, Andrea; Schirmer, Kristin; Sforzini, Susanna; Sturzenbaum, Stephen R.; Softeland, Liv; Turk, Valentina; Viarengo, Aldo; Werner, Inge; Yagur-kroll, Sharon; Zounkova, Radka; Lettieri, Teresa.
The risk posed by complex chemical mixtures in the environment to wildlife and humans is increasingly debated, but has been rarely tested under environmentally relevant scenarios. To address this issue, two mixtures of 14 or 19 substances of concern (pesticides, pharmaceuticals, heavy metals, polyaromatic hydrocarbons, a surfactant, and a plasticizer), each present at its safety limit concentration imposed by the European legislation, were prepared and tested for their toxic effects. The effects of the mixtures were assessed in 35 bioassays, based on 11 organisms representing different trophic levels. A consortium of 16 laboratories was involved in performing the bioassays. The mixtures elicited quantifiable toxic effects on some of the test systems...
Tipo: Text Palavras-chave: Bioassays; Effects; Mixtures; Ecotoxicology; Biomarkers.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00499/61115/64667.pdf
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Inhibición del crecimiento de chlamydomonas sp. por la sal isopropilamina de n-(fosfonometil)glicina OceanDocs
Albarracín, I.; Pío, G.; Salomón, R.; Cravero, M..
Los bioensayos con microalgas como bioindicadores de ecotoxicidad son útiles para evaluar el impacto ambiental provocado por las descargas de productos químicos y el vertido de efluentes. Siendo el fitoplancton el primer eslabón de la cadena trófica en los ecosistemas acuáticos, cualquier alteración de la composición de la comunidad microalgal, producto de estrés toxicológico, modifica la estructura y la función de todo el sistema. El Río Chubut, en Argentina, abastece de agua a varias localidades; se utiliza para el riego de superficies cultivadas y la generación de energía eléctrica. Su curso puede recibir filtraciones, impactando en la calidad higiénico-sanitaria de sus aguas. En el Valle Inferior del río Chubut (VIRCH) se utilizan diversos pesticidas...
Tipo: Preprint Palavras-chave: Bioassays; Bioassays; Http://aims.fao.org/aos/agrovoc/c_15731.
Ano: 2010 URL: http://hdl.handle.net/1834/3640
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Toxicidad de residuales líquidos de un central azucarero sobre la langosta espinosa Panulirus argus, Latreille, 1804 OceanDocs
Suárez, G.; Romero, T.; Perigo, E..
1 The waste waters of the sugar industry, have constituted the main organic focuses of pollution of the Cuban coastal waters, during the years 1958 to 1991 worked around 160 sugar mill factories with a production average between 3,5 and 8,0 million metric ton sugar cane. During the last years this industry sensibly diminished its activity being considered the production of residual in 70 %, only working 25 % of the factories installed in Cuba, implying certain reduction of the sea impact. This report tries to interpret by means of toxicity bioassays the effects of waste waters of this industry on the survival of the spiny lobster Panulirus argus. Being that the values of LC50 were of 6,7; 5,6; 3,2 and 2,7 % for 24, 48, 72 and 96 h to the contact of...
Tipo: Preprint Palavras-chave: Bioassays; Bioassays; Http://aims.fao.org/aos/agrovoc/c_15731.
Ano: 2010 URL: http://hdl.handle.net/1834/3539
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Aplicación estadística en los bioensayos de toxicidad con sedimentos extraído del Estero Salado, canal de acceso al Puerto Marítimo de Guayaquil OceanDocs
Villamar, F..
With the purpose of proving and determining in a short term the impact of the dredged material that can be caused by the organisms that inhabit the marine-estuary ecosystem, was performed toxicity bioassay of the sediment extracted from the bottom of the Salado Estuary. This sediment obstructs the access channels of the Marítime Port of Guayaquil. For this bioassay, was used white shrimp larvae of the species Litopenaeus vannamei, a commercially important organism that depends for its food on the organic detritus coming from the sediment and the column of estuary water in the studied area. The results obtained were analyzed applying the graphic arithmetic method and statistical analysis, through which it was determined that there is a direct relation...
Tipo: Journal Contribution Palavras-chave: Bioassays; Toxicity; Marine organisms; Sediments; Statistical analysis; Kurtosis; Dredged samples; Bioassays; Toxicity; Statistical analysis; Http://aims.fao.org/aos/agrovoc/c_15731; Http://aims.fao.org/aos/agrovoc/c_7826; Http://aims.fao.org/aos/agrovoc/c_7378.
Ano: 2002 URL: http://hdl.handle.net/1834/2257
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Los Bioensayos de Toxicidad en Sedimentos Marinos OceanDocs
Carballo Hondal, O.; Arencibia Carballo, G.; Concepción, J.; Isla Molleda, M..
Many organic and in organic contaminants originated from human activities are deposited and concentrated in aquatic sediments. The purpose of this review article is to analyze and discuss, the objectives, procedures and applications of bioassays on the triad of marine sediments, aiming at a reflection on the possibility of implementing regulations on the environmental monitoring of these studies in our country, so as to improve the environmental quality and its management on coastal zones. The main objective is to highlight the understanding of the adequate application and interpretation of bioassays. A summary of reports is presented, knowledge and analysis concerning marine sediments including, the chemical analysis, the direct determination of...
Tipo: Journal Contribution Palavras-chave: Toxicity; Sediments; Bioassays; Toxicity; Bioassays; Http://aims.fao.org/aos/agrovoc/c_7826; Http://aims.fao.org/aos/agrovoc/c_15731.
Ano: 2010 URL: http://hdl.handle.net/1834/3670
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Comparación experimental de las cepas colombianas de Artemia (Galerazamba, Manaure y Pozos Colorados) OceanDocs
Newmark-Umbreit, F..
Se examinaron en el laboratorio las influencias de la densidad de siembra y la salinidad en la producción de biomasa, el crecimiento y la madurez sexual de cepas Colombianas de Anemia. Los resultados indican (P<0.05) que cuando se combinan densidades de siembra bajas (10 na up I ios/1 ¡tro) y salinidades de 130°/oo, se incrementa el crecimiento, la madurez sexual, y la producción de biomasa de las tres cepas estudiadas. La cepa de Galerazamba se apartó un poco de dicho comportamiento al presentar producciones importantes de biomasa y de hembras maduras sexualmentea salinidades de 130°/ooy densidades de 50 nauplios/litro. Finalmente, con base en las diferencias biométricas y el comportamiento del cultivo experimental, se concluye que la cepa de...
Tipo: Journal Contribution Palavras-chave: Interspecific relationships; Marine crustaceans; Carcinology; Bioassays; Salinity effects; Biomass; Density; Bioassays; Biomass; Density; Http://aims.fao.org/aos/agrovoc/c_15731; Http://aims.fao.org/aos/agrovoc/c_926; Http://aims.fao.org/aos/agrovoc/c_2186.
Ano: 1990 URL: http://hdl.handle.net/1834/3888
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Bioensayo para calcular el CL50 del dispersante de petróleo BP 1100-WD con larvas de camarón Penaeus vannamei OceanDocs
Villamar, F..
En el presente estudio se realiza un bioensayo para determinar el grado de concentración media letal del dispersante de petróleo BP 1100-WD, utilizando organismos vivos en condiciones de laboratorio. Se describe el método estadístico de Bliss. En definitiva el propósito de realizar bioensayos es la de comparar la toxicidad de diferentes compuestos y conocer la sensibilidad de las diversas especies, para determinar los mecanismos de los efectos de las sustancias ensayadas (Alcazar, 1988).
Tipo: Journal Contribution Palavras-chave: Bioassays; Toxicity; Larvae; Shrimp fisheries; Bioassays; Toxicity; Larvae.
Ano: 1990 URL: http://hdl.handle.net/1834/2164
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Inhibición del crecimiento de Chlamydomonas sp. por la sal isopropilamina de N-(fosfonometil) glicina OceanDocs
Albarracín,I.; Pío, G.; Salomón, R.; Cravero, M..
El hombre, en el afán de una agricultura más eficiente, permanentemente procura desarrollar productos plaguicidas, sin tener en cuenta que su uso indiscriminado provoca adaptaciones en plagas, tornándolas más resistentes. En el Valle Inferior del Río Chubut (VIRCH), provincia del Chubut, Argentina, los agricultores utilizan herbicidas organofosforados como el glifosato (sal isopropilamina del N-fosfonometil glicina), para el control de malezas en diversos cultivos, asumiendo el riesgo que implican las filtraciones en la calidad higiénico-sanitaria de sus aguas. Dado que las microalgas responden rápidamente a cambios ambientales debido a sus tiempos cortos de generación y a su papel como productores primarios en los ecosistemas acuáticos, se usan en ensayos...
Tipo: Journal Contribution Palavras-chave: Bioassays; Toxicity.
Ano: 2011 URL: http://hdl.handle.net/1834/4629
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Bioensayo de toxicidad (CL50) del dispersante de petróleo BP 1100 WD, con fitoplancton marino (Tetraselmis sp) OceanDocs
Villamar, F..
El presente estudio se considera de carácter preliminar especialmente en lo referente al cálculo de la concentración media letal (CL50), de esta manera se evalúa el grado de toxicidad del dispersante de petróleo BP 1100 WD considerado como agente contaminante cuando se utiliza para dispersar manchas de petróleo regado en el mar. Con esta información se establece el impacto potencial que puede producir este dispersante en el medio ambiente marino.
Tipo: Journal Contribution Palavras-chave: Bioassays; Toxicity; Phytoplankton; Dispersants; Pollution indicators; Toxicity; Phytoplankton; Dispersants.
Ano: 1996 URL: http://hdl.handle.net/1834/2191
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