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| Registros recuperados: 28 | |
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| Zeraik,Ana Eliza; Nitschke,Marcia. |
| Bacterial adhesion to inert surfaces is a complex process influenced by environmental conditions. In this work, the influence of growth medium and temperature on the adhesion of Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Micrococcus luteus and Listeria monocytogenes to polystyrene surfaces was studied. Most bacteria demonstrated the highest adhesion when cultured in TSYEA, except S. marcescens, which showed to be positively influenced by the pigment production, favored in poor nutrient media (lactose and peptone agar). P. aeruginosa adhesion to polystyrene increased at low temperatures whatever the medium used. The culture medium influenced the surface properties of the bacteria as assessed by the MATS test. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial adhesion; Biofilms; Hydrophobicity; Polystyrene. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400012 |
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| Rocha,Antônio José; Barsottini,Mario Ramos de Oliveira; Rocha,Renan Rhonalty; Laurindo,Maria Vitória; Moraes,Francisco Leandro Laurindo de; Rocha,Soraya Lília da. |
| Abstract In this review, we explore some aspects of Pseudomonas aeruginosa virulence factors that are related to disease development in healthy organisms and resistance to antibiotics. This pathogen is one of the most clinically and epidemiologically important bacteria in Brazil, being the major cause of opportunistic infections. Among the virulence factors, biofilm formation acting of manner different in the organism. Furthermore, we review several P. aeruginosa genes that act in antimicrobial resistance, such as β-lactamases against β-lactamers. The resistance to pied-lactamases in P. aeruginosa is associated to resistance to the broad-spectrum cephalosporin. On the other hand, there is a group of synthetic broad-spectrum antibiotics acting on DNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pseudomonas aeruginosa; Virulence factors; Antimicrobial activities; Biofilms; Β-lactamase. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100902 |
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| Hassan,Afreenish; Usman,Javaid; Kaleem,Fatima; Omair,Maria; Khalid,Ali; Iqbal,Muhammad. |
| BACKGROUND: Microorganisms growing in a biofilm are associated with chronic and recurrent human infections and are highly resistant to antimicrobial agents. There are various methods to detect biofilm production like Tissue Culture Plate (TCP), Tube method (TM), Congo Red Agar method (CRA), bioluminescent assay, piezoelectric sensors, and fluorescent microscopic examination. OBJECTIVE: This study was conducted to compare three methods for the detection of biofilms. METHOD: The study was carried out at the Department of Microbiology, Army Medical College, National University of Sciences and Technology, Pakistan, from January 2010 to June 2010. A total of 110 clinical isolates were subjected to biofilm detection methods. Isolates were identified by standard... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Bacteria; Anti-bacterial agents. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400002 |
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| Aparna,Madhu Sharma; Yadav,Sarita. |
| Bacteria that attach to surface aggregate in a hydrated polymeric matrix of their own synthesis to form biofilms. These represent microbial societies with their own defense and communication system. Transitioning from acute to chronic infection is frequently associated with biofilm formation.Bacteria in biofilms are innately more resistant to antimicrobial agents. The presence of indwelling medical devices increases the risk for biofilm formation and subsequent infection. The current antibiotic therapies are of limited effectiveness in resolving biofilms infection.This review attempts to discuss the stages in biofilm formation, their pathogenic mechanisms, effect of antimicrobial agents, detection and eradication of the biofilms. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Staphylococci; Pseudomonas; Candida. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600016 |
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| Lourenço,Talita Gomes Baêta; Heller,Débora; Souto,Renata Martins do; Silva-Senem,Mayra Xavier e; Varela,Victor Macedo; Torres,Maria Cynesia Barros; Feres-Filho,Eduardo Jorge; Colombo,Ana Paula Vieira. |
| This study evaluates the antimicrobial susceptibility and composition of subgingival biofilms in generalized aggressive periodontitis (GAP) patients treated using mechanical/antimicrobial therapies, including chlorhexidine (CHX), amoxicillin (AMX) and metronidazole (MET). GAP patients allocated to the placebo (C, n = 15) or test group (T, n = 16) received full-mouth disinfection with CHX, scaling and root planning, and systemic AMX (500 mg)/MET (250 mg) or placebos. Subgingival plaque samples were obtained at baseline, 3, 6, 9 and 12 months post-therapy from 3–4 periodontal pockets, and the samples were pooled and cultivated under anaerobic conditions. The minimum inhibitory concentrations (MICs) of AMX, MET and CHX were assessed using the microdilution... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aggressive periodontitis; Biofilms; Microbial sensitivity tests; DNA probes. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200493 |
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| Reis-Teixeira,Fernanda Barbosa dos; Alves,Virgínia Farias; Martinis,Elaine Cristina Pereira de. |
| Abstract The pathogenic bacterium Listeria monocytogenes can persist in food processing plants for many years, even when appropriate hygienic measures are in place, with potential for contaminating ready-to-eat products and, its ability to form biofilms on abiotic surfaces certainly contributes for the environmental persistence. In this research, L. monocytogenes was grown in biofilms up 8 days attached to stainless steel and glass surfaces, contributing for advancing the knowledge on architecture of mature biofilms, since many literature studies carried out on this topic considered only early stages of cell adhesion. In this study, biofilm populations of two strains of L. monocytogenes (serotypes 1/2a and 4b) on stainless steel coupons and glass were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Listeria monocytogenes; Abiotic surfaces; Persistence; Biofilms. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300587 |
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| Melo,Poliana de Castro; Ferreira,Luciano Menezes; Nader Filho,Antônio; Zafalon,Luiz Francisco; Vicente,Hinig Isa Godoy; Souza,Viviane de. |
| Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Bovine mastitis; Phenotypic and molecular analysis; Staphylococcus aureus. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100017 |
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| Cândido,Cinthya S.; Portella,Cadmo Silton A.; Laranjeira,Bruno J.; Silva,Sérgio S. da; Arriaga,Angela M.C.; Santiago,Gilvandete M. P.; Gomes,Geovany A.; Almeida,Paulo César; Carvalho,Cibele B. M.. |
| The essential oil from the leaves of Myrcia ovata Cambess., commonly used in Brazil for the treatment of gastric illnesses, was screened for antimicrobial activity and action in the formation of microbial biofilms by Enterococcus faecalis. The oil was obtained by hydrodistillation using a clevenger-type system. Its chemical composition was analyzed using GC and GC-MS. Both MIC and MBC of the essential oil were determined by broth microdilution techniques and agar dilution method. The essential oil showed antimicrobial activity against E. faecalis, Escherichia coli, Pseudomonas aeruginosa, Salmonella choleraesuis, Staphylococcus aureus, Streptococcus pneumoniae and Candida parapsilosis. The results showed that the essential oil of M. ovata Cambess. was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Essential oil; Myrcia ovata Cambess.; Antimicrobial activity; Biofilms. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300012 |
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| Iñiguez-Moreno,Maricarmen; Gutiérrez-Lomelí,Melesio; Guerrero-Medina,Pedro Javier; Avila-Novoa,María Guadalupe. |
| Abstract The aim of this study was evaluated the biofilm formation by Staphylococcus aureus 4E and Salmonella spp. under mono and dual-species biofilms, onto stainless steel 316 (SS) and polypropylene B (PP), and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite. The biofilms were developed by immersion of the surfaces in TSB by 10 d at 37 °C. The results showed that in monospecies biofilms the type of surface not affected the cellular density (p > 0.05). However, in dual-species biofilms on PP the adhesion of Salmonella spp. was favored, 7.61 ± 0.13 Log10 CFU/cm2, compared with monospecies biofilms onto the same surface, 5.91 ± 0.44 Log10 CFU/cm2 (p < 0.05). The mono and dual-species biofilms were subjected to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Salmonella spp.; Staphylococcus aureus; Disinfectants; Sensitivity. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200310 |
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| Oliveira,Ana Claudia de; Maluta,Renato Pariz; Stella,Ariel Eurides; Rigobelo,Everlon Cid; Marin,José Moacir; Ávila,Fernando Antonio de. |
| A wide variety of opportunistic pathogens has been detected in the tubing supplying water to odontological equipment, in special in the biofilm lining of these tubes. Among these pathogens, Pseudomonas aeruginosa, one of the leading causes of nosocomial infections, is frequently found in water lines supplying dental units. In the present work, 160 samples of water, and 200 fomite samples from forty dental units were collected in the city of Barretos, State of São Paulo, Brazil and evaluated between January and July, 2005. Seventy-six P. aeruginosa strains, isolated from the dental environment (5 strains) and water system (71 strains), were tested for susceptibility to six antimicrobial drugs most frequently used against P. aeruginosa infections.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pseudomonas aeruginosa; Dental units; Water lines; Opportunistic pathogens; Antimicrobial resistance; Biofilms. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300032 |
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| Tavares,Luciana Lopes; Almeida,Crislene Barbosa de; Caruso,Ícaro Putinhon; Cornélio,Marinônio Lopes; Lopes Filho,José Francisco. |
| The purpose of this study was to evaluate changes in the structure and some functional properties of biofilms added with modified clays (Cloisite® 15A and Cloisite® 30B) prepared by the casting method. The analysis of the microstructure of the films, scanning electron microscopy (SEM), Optical microscopy (MO), and Infrared Spectroscopy (FTIR) indicated that the addition of clay in the films resulted in the formation of a heterogeneous microstructure, microcomposite or tactoid. Due to the formation of a microcomposite structure, functional properties of the films added with both clays such as opacity, solubility, and permeability to water vapor (PVA), were not better than those of the control film. Thus, it was concluded that although it is possible to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Zein; Clays; Microcomposite; Tactoids. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000200017 |
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| Webber,Bruna; Oliveira,Amauri Picollo de; Pottker,Emanuele Serro; Daroit,Luciane; Levandowski,Rafael; Santos,Luciana Ruschel dos; Nascimento,Vladimir Pinheiro do; Rodrigues,Laura Beatriz. |
| ABSTRACT: We evaluated the influence of temperature on the ability of Salmonella Enteritidis (SE) to form biofilms on stainless steel, polyethylene, and polyurethane surfaces under different hygiene procedures. These materials were placed on SE culture and incubated at 42±1 ºC, 36±1 ºC, 25±1 ºC, 9±1 ºC, and 3±1 ºC for 4, 8, 12, and 24 h. Hot water at 45 ºC and 85 ºC, 0.5% peracetic acid solution, and 1% quaternary ammonia were used for hygienization. Biofilm formation occurred at all temperatures evaluated, highlighting at 3 ºC which has not been reported as an ideal temperature for the adhesion of SE to these materials. The SE adhered more often to polyethylene surfaces than to polyurethane and stainless steel surfaces (P<0.05). Peracetic acid and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella Enteritidis; Biofilms; Surfaces; Hygiene procedures; Food microbiology. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000700452 |
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| Registros recuperados: 28 | |
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