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| Registros recuperados: 20 | |
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| WISINTAINER,GABRIELLE G.N.S.; SIMÕES,EVELYNE R.B.; LEMOS,TELMA L.G.; MOURA,SIDNEI; SOUZA,LUCIANA G.S.; FONSECA,ALUISIO M.; MORAES,MANOEL ODORICO; PESSOA,CLAUDIA; ROESCH-ELY,MARIANA; HENRIQUES,JOÃO A.P.. |
| Biflorin is an o-naphthoquinone with proven cytotoxic effects on tumor cells showing antimicrobial, antitumor and antimutagenic activities. Biflorin is an isolated compound taken from the roots of the plantCapraria biflora L. (Schrophulariaceae), indigenous of the West Indies and South America, which is located in temperate or tropical areas. This compound has shown to be strongly active against grampositive and alcohol-acid-resistant bacteria. It has been efficient in inhibiting the proliferation tumor cell lines CEM, HL-60, B16, HCT-8 and MCF-7. Recently, SK-Br3 cell line was treated with biflorin showing important cytotoxic effects. In this article, information related to the first structural characterization studies are presented, as well as the latest... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological activity; Biflorin; Capraria biflora; Natural products. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401907 |
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| ALENCAR,DANIEL B.; MELO,ARTHUR A.; SILVA,GISELLE C.; LIMA,REBECA L.; PIRES-CAVALCANTE,KELMA M.S.; CARNEIRO,RÔMULO F.; RABELO,ADRIANA S.; SOUSA,OSCARINA V.; VIEIRA,REGINE H.S.F.; VIANA,FRANCISCO A.; SAMPAIO,ALEXANDRE H.; SAKER-SAMPAIO,SILVANA. |
| Marine invertebrates are capable of synthesizing bioactive compounds, which may be beneficial to human health. The aim of this study was to evaluate the antioxidant, hemolytic, antimicrobial and cytotoxic activities of crude extract (70% EtOH), and dichloromethane (DCM), ethyl acetate (EtOAc), and aqueous (Aq) fractions of the marine zoanthid Palythoa caribaeorum. The phenolic compound contents of the crude extract, DCM, EtOAc and Aq fractions were 12.33, 18.17, 10.53, and 3.18 mg GAE per gram, respectively. DPPH radical scavenging activity showed slight variation. IC50 of crude extract, DCM, EtOAc and Aq fractions were 11.13, 11.25, 11.74, and 11.28 µg mL-1, respectively. Among the sample, ferrous ion chelating was the highest in crude extract (IC50... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological activity; Marine invertebrate; Natural product; Sphenopidae. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000201113 |
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| Chen,Feng; Chen,Xiang-Ming; Chen,Zhi; Jiang,Han-Liang; Pan,Xiao-Ping; Hu,Zhong-Rong; Liu,Rong-Hua; Chen,Xiao-Ming. |
| We want to construct a yeast expression system for thymosin a1 (Ta1) to make the orally administered Ta1 preparation possible. The whole Ta1 DNA fragment was obtained by PCR. After being digested with restriction enzymes, it was cloned into pYES2 vector. Sequencing was performed to identify the recombinant. The sequence of Ta1 in recombinant coincided with the original one reported in Genbank. When pYES2-Ta1 plasmid was transformed into yeast, galactose instead of glucose was used to induce Ta1 expression. Western blot was performed to identify the quality of the expressed Ta1. Dried yeast containing pYEST2-Ta1 was fed to Balb/c mice whose immunities were inhibited by cyclophosphamide in advance. Synthesized Ta1 peptide was used as positive control and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Thymosin a1; Yeast expression system; Biological activity; Orally administered. |
| Ano: 2005 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300002 |
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| Carbonell,G.V.; Amorim,C.R.N.; Furumura,M.T.; Darini,A.L.C.; Fonseca,B.A.L.; Yano,T.. |
| Serratia marcescens cytotoxin was purified to homogeneity by ion-exchange chromatography on a DEAE Sepharose Fast Flow column, followed by gel filtration chromatography on a Sephadex G100 column. The molecular mass of the cytotoxin was estimated to be about 50 kDa. Some biological properties of the cytotoxin were analyzed and compared with well-characterized toxins, such as VT1, VT2 and CNF from Escherichia coli and hemolysin produced by S. marcescens. The sensitivity of the cell lines CHO, HeLa, HEp-2, Vero, BHK-21, MA 104 and J774 to the cytotoxin was determined by the cell viability assay using neutral red. CHO and HEp-2 were highly sensitive, with massive cellular death after 1 h of treatment, followed by BHK-21, HeLa, Vero and J774 cells, while MA 104... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cell culture; Biological activity; Serratia marcescens; Cytotoxin; Virulence factors. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300010 |
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| Sun,H.; Wu,G.M.; Chen,Y.Y.; Tian,Y.; Yue,Y.H.; Zhang,G.L.. |
| Intercellular adhesion molecule-1 (ICAM-1) is an important factor in the progression of inflammatory responses in vivo. To develop a new anti-inflammatory drug to block the biological activity of ICAM-1, we produced a monoclonal antibody (Ka=4.19×10−8 M) against human ICAM-1. The anti-ICAM-1 single-chain variable antibody fragment (scFv) was expressed at a high level as inclusion bodies in Escherichia coli. We refolded the scFv (Ka=2.35×10−7 M) by ion-exchange chromatography, dialysis, and dilution. The results showed that column chromatography refolding by high-performance Q Sepharose had remarkable advantages over conventional dilution and dialysis methods. Furthermore, the anti-ICAM-1 scFv yield of about 60 mg/L was higher with this method. The purity... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Intercellular adhesion molecule-1; Single-chain variable antibody fragment; Expression; Purification; Renaturation; Biological activity. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700540 |
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| Lima,E.B.C.; Sousa,C.N.S.; Meneses,L.N.; Ximenes,N.C.; Santos Júnior,M.A.; Vasconcelos,G.S.; Lima,N.B.C.; Patrocínio,M.C.A.; Macedo,D.; Vasconcelos,S.M.M.. |
| Cocos nucifera (L.) (Arecaceae) is commonly called the “coconut tree” and is the most naturally widespread fruit plant on Earth. Throughout history, humans have used medicinal plants therapeutically, and minerals, plants, and animals have traditionally been the main sources of drugs. The constituents of C. nucifera have some biological effects, such as antihelminthic, anti-inflammatory, antinociceptive, antioxidant, antifungal, antimicrobial, and antitumor activities. Our objective in the present study was to review the phytochemical profile, pharmacological activities, and toxicology of C. nucifera to guide future preclinical and clinical studies using this plant. This systematic review consisted of searches performed using scientific databases such as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cocos nucifera (L.); Ethnopharmacology; Pharmacological properties; Biological activity; Review. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001100953 |
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| Damasceno,Carolina Sette Barbosa; Oliveira,Letícia Freire de; Szabo,Ellis Marina; Souza,Ângela Maria; Dias,Josiane Fatima Gaspari; Miguel,Marilis Dalarmi; Miguel,Obdúlio Gomes. |
| ABSTRACT The essential oil composition of the Ocotea bicolor, native plant of Brazil, was studied for the first time. The essential oil of the leaf was obtained by hydrodistillation and analyzed by GC/MS. The analytical procedure revealed a predominance of sesquiterpenes, δ-cadinene (7.39%), β-sesquiphellandrene (6.67%), β-elemene (5.41%) and α-cadinol (5,23%). The essential oil was submitted to brine shrimp toxicity evaluation, antioxidant and antibacterial tests. The antioxidant activity by the formation of phosphomolybdenum complex method presented positive results. The minimum inhibitory concentration (MIC) values were higher than 1000 μg/mL for the microorganisms tested. Toxicity activity revealed LC50 results of 40.10 (μg/mL), being toxic to the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ocotea bicolor/Oil essential/chemical composition; Lauraceae; Antioxidant activity; Biological activity. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400615 |
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| Yaldiz,Gulsum; Yildirim,Arzu Birinci; Arici,Yeliz Kasko; Camlica,Mahmut. |
| G. Yaldiz, A. B. Yildirim, Y. K. Arici, and M. Camlica. 2016. Yield, yield features, phytochemical composition, antioxidant and antibacterial activities of Abutilon indicum cultivated under different fertilizers. Cien. Inv. Agr. 43(3):464-475. Abutilon indicum (Link) Sweet is an important medicinal plant that has been used in traditional medicine for centuries, and it is one ofthe novel crops being used in the pharmaceutical industry. Agricultural practices that produce high drug yield and desired secondary metabolites are in high demand by pharmaceutical-related industries; therefore, the aim of this study was to determine the leaf and seed yield, crude oil yield, crude oil composition, total phenolic content, and the antioxidant and antibacterial... |
| Tipo: Journal article |
Palavras-chave: Biological activity; CAN; Crude oil; Fertilizer; NPK; Yield; Aceite crudo; Actividad biológica; CAN; Fertilizante; NPK; Rendimiento. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000300012 |
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| CRUZ-RAMÍREZ,Susana-Gabriela; LÓPEZ-SAIZ,Carmen-María; ROSAS-BURGOS,Ema-Carina; CINCO-MOROYOQUI,Francisco-Javier; VELÁZQUEZ,Carlos; HERNÁNDEZ,Javier; BURGOS-HERNÁNDEZ,Armando. |
| Abstract The search for chemopreventive/chemoprotective compounds in marine organism has been extensively reported; however, the presence of these compounds in octopus has been incipiently explored. In this research, the antimutagenic, antiproliferative, and antioxidant potential of three crude extracts (methanolic, acetonic, and hexanic) from Paroctopus limaculatus was investigated. Antimutagenic activity against aflatoxin B1 (AFB1) was evaluated through the Ames test using Salmonella typhimurium tester strains TA98 and 100. Antiproliferative activity was assessed using the standard MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) assay on M12.C3.F6 murine cell line. Antioxidant activity was assessed using the DPPH... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Octopus; Biological activity; Chemopreventive. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000400722 |
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| Cuvertino-Santoni,Jorge; Montenegro,Gloria. |
| Here, we present the current state of knowledge on the diversity of Chilean bryophytes (mosses, liverworts, and hornworts), the secondary metabolites present in these plants, and the biological activity of extracts from populations of Chilean species. Our goal is to establish the potential of these plants as a source of useful molecules for pharmaceutical and agricultural industries, thus promoting their conservation. Only 3,7% of Chilean bryophytes (55 spp.) have been analyzed using chemical characterization of their extracts (methanol, ether, ethanol, dichloromethane, acetone and hexane). Only four species from Chilean and Argentinian populations (Porella chilensis (Lehm. & Lindenb.) Trevi., Riccardiapolyclada (Mitt. ex Thurn) Hässel,... |
| Tipo: Journal article |
Palavras-chave: Mosses; Liverworts; Hornworts; Uses; Secondary metabolites; Biological activity; In vitro cultivation. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432013000100002 |
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| Senni, Karim; Pereira, Jessica; Gueniche, Farida; Delbarre-ladrat, Christine; Sinquin, Corinne; Ratiskol, Jacqueline; Godeau, Gaston; Fischer, Anne-marie; Helley, Dominique; Colliec-jouault, Sylvia. |
| The therapeutic potential of natural bioactive compounds such as polysaccharides, especially glycosaminoglycans, is now well documented, and this activity combined with natural biodiversity will allow the development of a new generation of therapeutics. Advances in our understanding of the biosynthesis, structure and function of complex glycans from mammalian origin have shown the crucial role of this class of molecules to modulate disease processes and the importance of a deeper knowledge of structure-activity relationships. Marine environment offers a tremendous biodiversity and original polysaccharides have been discovered presenting a great chemical diversity that is largely species specific. The study of the biological properties of the... |
| Tipo: Text |
Palavras-chave: Marine bacteria; Marine algae; Exopolysaccharides; Sulfated polysaccharides; Structure; Chemical modification; Biological activity; Blue biotechnology; Cell therapy; Tissue engineering. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00048/15894/13316.pdf |
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| Falaise, Charlotte; Francois, Cyrille; Travers, Marie-agnes; Morga, Benjamin; Haure, Joel; Tremblay, Rejean; Turcotte, Francois; Pasetto, Pamela; Gastineau, Romain; Hardivillier, Yann; Leignel, Vincent; Mouget, Jean-luc. |
| The search for novel compounds of marine origin has increased in the last decades for their application in various areas such as pharmaceutical, human or animal nutrition, cosmetics or bioenergy. In this context of blue technology development, microalgae are of particular interest due to their immense biodiversity and their relatively simple growth needs. In this review, we discuss about the promising use of microalgae and microalgal compounds as sources of natural antibiotics against human pathogens but also about their potential to limit microbial infections in aquaculture. An alternative to conventional antibiotics is needed as the microbial resistance to these drugs is increasing in humans and animals. Furthermore, using natural antibiotics for... |
| Tipo: Text |
Palavras-chave: Biological activity; Antimicrobial; Antibacterial; Antifungal; Antiviral; Biological activity; Haslea; Microalgae; Sustainable aquaculture. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00349/46052/45719.pdf |
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| Guizien, Katell; Orvain, Francis; Duchene, Jean-claude; Le Hir, Pierre. |
| The average bed shear stress and bed friction factor of samples with any roughness was derived from the head loss between upstream and downstream of a test section in an erosion tunnel. The method was validated in both hydraulically smooth (plexiglass; Reynolds number less than 25,000) and rough regimes (calibrated particles with known roughness). As a first step toward using this method on natural sediment, this method was tested with experimental mesocosms assembled from field collected materials (sieved sediments; diatoms). Bed shear stress measurement precision was high enough in the experiments to detect a positive significant relationship between bed friction factor and core roughness. The observed bed friction factor increase could be related to... |
| Tipo: Text |
Palavras-chave: Bed roughness; Friction factor; Mud beds; Biological activity; Erosion experiments. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00117/22807/20617.pdf |
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| Balota,Elcio Liborio; Yada,Ines Fumiko Ubukata; Amaral,Higo Furlan; Nakatani,Andre Shigueyoshi; Hungria,Mariangela; Dick,Richard Peter; Coyne,Mark Steven. |
| Many forested areas have been converted to intensive agricultural use to satisfy food, fiber, and forage production for a growing world population. There is great interest in evaluating forest conversion to cultivated land because this conversion adversely affects several soil properties. We examined soil microbial, physical, and chemical properties in an Oxisol (Latossolo Vermelho distrófico) of southern Brazil 24 years after forest conversion to a perennial crop with coffee or annual grain crops (maize and soybeans) in conventional tillage or no-tillage. One goal was to determine which soil quality parameters seemed most sensitive to change. A second goal was to test the hypothesis that no-tillage optimized preservation of soil quality indicators in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Land use; Tillage; Coffee; Biological activity; Potential mineralization; Aggregate stability. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000401003 |
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| Registros recuperados: 20 | |
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