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Methodological limitations of counting total leukocytes and thrombocytes in reptiles (Amazon turtle, Podocnemis expansa): an analysis and discussion Acta Amazonica
Tavares-Dias,Marcos; Oliveira-Júnior,Antônio Aderson; Marcon,Jaydione Luiz.
The aim of this paper is to compare three different methods for counting white blood cells [WBC] (Natt and Herrick method, estimation with 1,000 and 2,000 erythrocytes) and three methods for counting total thrombocytes [TT] (Wojtaszek method, estimation with 1,000 and 2,000 erythrocytes) in a South American freshwater turtle species, Podocnemis expansa, Schweigger 1812 (Reptilia, Pelomedusidae). Direct WBC counts using the Natt and Herrick method showed limitations, which are discussed here. The WBC and TT counts using 1,000 erythrocytes from blood smears are not recommended for Amazon turtles nor other reptilian species, since wide variation in counts can be observed. Estimation methods for determining WBC and TT based on 2,000 erythrocytes of blood...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amazon turtles; Blood; Leukocytes; Podocnemis expansa; Thrombocytes.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000200020
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Comparative study on hematological parameters of farmed matrinxã, Brycon amazonicus Spix and Agassiz, 1829 (Characidae: Bryconinae) with others Bryconinae species Acta Amazonica
Tavares-Dias,Marcos; Affonso,Elizabeth Gusmão; Oliveira,Sarah Ragonha; Marcon,Jaydione Luiz; Egami,Mizue Imoto.
The aim of this study was not only to determine the red blood cells parameters, thrombocyte and leukocyte counts in farmed Brycon amazonicus (matrinxã), to compare these parameters among Bryconinae species from literature, and also to investigate the presence of special granulocytic cells in these fish. The results of the blood cells parameters here established for farmed B. amazonicus, a species of great economic importance in Brazilian aquaculture, could help a better understanding of the blood features in natural populations of this Amazon species. Blood parameters varied between Bryconinae species investigated, mainly the red blood cell counts, hemoglobin, hematocrit and mean corpuscular volume (MCV). The presence of the blood granulocytes, neutrophils...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leukocytes; Freshwater fish; Blood; Amazon; Brycon amazonicus; Bryconinae.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000400026
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HEMATOLOGYCAL AND BLOOD BIOCHEMISTRY PARAMETERS OF CAPTIVE BIG-HEADED AMAZON RIVER TURTLES, Peltocephalus dumerilianus (TESTUDINES: PODOCNEMIDIDAE) Acta biol.Colomb.
ANSELMO,Neiana Pereira; FRANÇA,Mônica Ferreira de Lima; SANTOS,Mareio Quara de Carvalho; PANTOJA-LIMA,Jackson; SILVA,Cleide Karoline Pereira da; ARIDE,Paulo Henrique Rocha; OLIVEIRA,Adriano Teixeira de.
ABSTRACT The determination of hematological values is used to obtain knowledge about the health conditions of animal species. The big-headed Amazon River turtles, (Peltocephalus dumerilianus) are considered one of the least known testudine species concerning their biology and health status. Herein, we determined the hematological and plasma biochemical parameters of17 (eight males and nine females) adult P. dumerilianus to provide reference interval values for clinically healthy individuals. We collected the blood samples by puncturing the femoral vein using long heparinized hypodermic syringes. Sexual dimorphism for individuals was determined by external observation of the shape of the plastron. The average values obtained for the ten hematological and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amazon; Blood; Hematology; Physiology; Reptiles.
Ano: 2021 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2021000200207
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Effects of in ovo injection of nano-selenium and nano-zinc oxide and high eggshell temperature during late incubation on antioxidant activity, thyroid and glucocorticoid hormones and some blood metabolites in broiler hatchlings Animal Sciences
Shokraneh, Meisam; Sadeghi, Ali Asghar; Mousavi, Seyed Naser; Esmaeilkhanian, Saeid; Chamani, Mohammad.
This experiment was conducted to evaluate the effects of in ovo injection of nano-selenium (Nano-Se) and nano-zinc oxide (Nano-ZnO) and high eggshell temperature (EST) during late incubation on blood parameters of broiler hatchlings. A total of 750 fertile eggs, were weighed and randomly distributed among 5 treatment groups on each of 5 replicate tray levels. The injection was performed on 17 d of incubation. Treatments included of: 1) Eggs not injected and incubated at normal EST (control); 2) Eggs not injected and incubated at high EST; 3) Eggs injected NaCl solution and incubated at high EST (sham); 4) Eggs injected NaCl solution containing 40 µg Nano-Se and incubated at high EST; 5) Eggs injected NaCl solution containing 500 µg Nano-ZnO and incubated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: In ovo injection; Nano-selenium; Nano-zinc oxide; High eggshell temperature; Blood.
Ano: 2020 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/46029
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Effect of dietary probiotic supplementation on carcass traits and haematological responses of broiler chickens fed shea butter cake based diets Animal Sciences
Aguihe, Paschal Chukwudi; Kehinde, Abiodun Solomon; Abdulmumini, Sufiyan; Ospina-Rojas, Iván Camilo; Murakami, Alice Eiko.
A 42-day study was conducted to investigate probiotic supplementation on shea butter cake (SBC) based diets of broilers on the carcass traits and haematological indices. A total of 280, 1-day old Arbor acres strain broiler chicks were divided into 7 dietary treatments with 4 replicates of 10 birds each in a completely randomized design. Diet 1 (control diet) has no SBC and probiotic supplementation while remaining 6 diets contained 5, 10, and 15% levels of SBC each with (+) or without (-) probiotic supplementation. The results showed that carcass parameters and prime cuts weights increased (p < 0.05) in birds fed probiotic-SBC diets compared to control diets. Abdominal fat content was lower (p < 0.05) in birds fed probiotic-SBC diets than those on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agro by-product; Feed additive; Carcass characteristics; Blood; Chicken..
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/34813
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Produto à base de própolis (LLOS) na dieta de bovinos inteiros confinados: comportamento animal e respostas sanguíneas - doi: 10.4025/actascianimsci.v33i1.10671 Animal Sciences
Faria, Leonardo Avanzzi Nunes; UEM; Barbosa, Orlando Rus; UEM; Zeoula, Lucia Maria; UEM; Aguiar, Silvia Cristina de; UEM; Prado, Rodolpho Martin do; UEM; Bertolini, Dennis Armando; UEM.
Avaliou-se o comportamento, as respostas sanguíneas de animais confinados recebendo dieta com produto à base de própolis (LLOS). Os tratamentos foram: controle (CON) e dois produtos à base de própolis (LLOSC1) e (LLOSC1+). Mediram-se os comportamentos: comendo (COM), bebendo água (BEB), andando (AND), ruminando em pé (RUEMP), ruminando deitado (RUDET), em pé (EMP) e deitado (DET). Maior tempo em COM, BEB, AND, RUEMP e EMP ocorreu de manhã (MA) e à tarde (TA), e em RUDET e DET à noite (NO) e madrugada (MD). Animais no Lado 1 do confinamento permaneceram mais tempo andando na MA e à NO, sem diferenças para MD, e mais tempo EMP para os do Lado 1 na MA, T e MD, e igual à NO. Cortisol, Glicose e Ureia não foram influenciados pelos tratamentos. Não houve efeito...
Tipo: Pesquisa de campo Palavras-chave: 5.04.01.00-9 comportamento animal; Confinamento; Própolis; Sangue animal behavior; Feedlot; Propolis; Blood.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/10671
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Changes in hematological biomarkers of Nellore cows at different reproductive stages Animal Sciences
Oliveira, Wagner Dias Coelho de; Dias e Silva, Tairon Pannunzio; Araújo, Marcos Jácome de; Edvan, Ricardo Loiola; Oliveira, Ronaldo Lopes; Bezerra, Leílson Rocha.
This study was conducted to evaluate changes in hematological biomarkers of Nellore cows at different reproductive stages. Fifteen multiparous cows with 4 ± 1 years of age and live weight of 400 ± 50 kg were used at different stages (non-pregnant, in the initial, middle and late pregnancy, at birth, one day postpartum, 30 and 60 days postpartum). Blood collections were performed every 30 days and assayed for the following hematological biomarkers: hemogram (red blood cells, globular volume, hemoglobin, mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC)) and leukogram (total and differential counts). The erythrogram was influenced (p < 0.001) by the reproductive stages, with variation in the results obtained for the total...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood; Bovine; Erythrogram; Leukogram; Postpartum; Prepartum..
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/45725
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Balanço eletrolítico e redução da proteína bruta da ração sobre parâmetros fisiológicos e sanguíneos de frangos de corte no calor - DOI: 10.4025/actascianimsci.v31i3.4792 Animal Sciences
Matos, Marize Bastos de; UENF; Ferreira, Rony Antonio; UNIVALE; Savaris, Vaneila Daniele Lenhardt; UENF; Couto, Humberto Pena; UENF; Soares, Rita da Trindade Ribeiro Nobre; UENF; Oliveira, Newton Tavares Escocard de; UENF.
Um experimento foi conduzido, objetivando-se avaliar o efeito do balanço eletrolítico (BER) e a redução da proteína bruta (PB) da ração sobre a frequência respiratória, temperatura cloacal, concentrações de Na+, K+, Cl- e ácido úrico em frangos de corte. Foram utilizados 800 pintos de corte, machos da linhagem Cobb, alojados em 20 boxes, distribuídos em delineamento inteiramente casualizado com quatro tratamentos, cinco repetições e 40 aves por unidade experimental. Os tratamentos foram: T1 - ração basal; T2 - ração corrigida para BER de 250 mEq kg-1; T3 - ração com redução de PB, sem correção de BER; T4 - ração com redução de PB corrigida para BER de 250 mEq kg-1. As condições ambientais evidenciaram que os animais estavam em estresse por calor. As aves...
Palavras-chave: 5.04.05.00-4 eletrólitos; Sangue; Homeostase; Estresse Ciencias Agrárias electrolytes; Homeostasis; Blood; Stress.
Ano: 2009 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/4792
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Protein, energetic, enzymatic and mineral profile of Nellore cows during the pregnancy, parturition and postpartum Animal Sciences
Oliveira, Wagner Dias Coelho de; Silva, Tairon Pannunzio Dias e; Bezerra, Leílson Rocha; Araújo, Marcos Jácome de; Edvan, Ricardo Loiola; Lopes Oliveira, Ronaldo.
The aim of this study was to evaluate the protein energetic, enzymatic and mineral profile of Nellore cows during the pregnancy, parturition and postpartum. Fifteen multiparous cows with 4 ± 1 years of age and live weight of 400 ± 50 kg were used at different stages (non-pregnant, in the initial, middle and late pregnancy, at birth, one day postpartum, 30 and 60 days postpartum). Blood collections were performed every 30 days and assayed for the following blood biomarkers: Protein (total proteins, albumin, urea and creatinine), energetic (cholesterol, triglycerides, glucose and beta hydroxybutyrate), mineral (calcium, phosphorus, magnesium) and enzymatic (alkaline phosphatase and aspartate aminotransferase). Calcium had the lowest concentrations (p...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biochemistry; Blood; Ketone bodies; Metabolites; Physiology. biochemistry; Blood; Ketone bodies; Metabolites; Physiology..
Ano: 2020 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/49022
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Haematology and melanoma crophage centers of Nile tilapia fed supplemented diet with propolis Biological Sciences
Ledic-Neto, Jerko; Universidade Federal de Santa Catarina; Dotta, Geovana; Universidade Federal de Santa Catarina; Garcia, Patrícia; Universidade Federal de Santa Catarina; Brum, Aline; Universidade Federal de Santa Catarina; Gonçalves, Eduardo Luis Tavares; Universidade Federal de Santa Catarina; Martins, Maurício Laterça; Universidade Federal de Santa Catarina.
The objective of this study was to investigate the influence of dietary supplementation with propolis on hematology and number and area of melanomacrophage centers in spleen and kidney of tilapia. After acclimation, fish (24.7 ± 7.4 gmean weight) were distributed in 6 tanks 100 L, 6 fish per tank, in triplicate, at a temperature 24.0 ± 2.8oC, with two treatments: Fish fed 2% propolis supplemented diet and fish fed non-supplemented diet. To monitor the evolution of the effects, two samples were collected: half of the fish from each treatment were used after 15 days and the other half after 21, composing two feeding times. After each feeding time, blood, spleen and kidney were collected. After twenty one days feeding on 2% propolis supplemented diet, fish...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.06.03.00-0 fish; Blood; Kidney; Spleen; Hemosiderin Aquicultura.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/22024
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Influence of low-protein diet with different levels of amino acids on laying hen performance, quality and egg composition Anais da ABC (AABC)
ALAGAWANY,MAHMOUD; EL-HINDAWY,MOHAMED M.; EL-HACK,MOHAMED E. ABD; ARIF,MUHAMMAD; EL-SAYED,SABRY ABD.
Abstract The present study aimed to investigate the consequences of dietary levels of crude protein (CP) and total sulfur amino acids (TSAA, Met+Cys) on productive performance, egg quality and egg components for Lohmann Brown laying hens through 18-34 weeks of age. A 2×3 factorial design experiment was conducted using two levels of CP (16 and 18 %) and three levels of Met+ Cys (0.67, 0.72 and 0.77 %). A total of 144 Lohmann Brown laying hens at 18 weeks of age were randomly divided into 6 treatment groups. The best values of final body weight and body weight change were recorded by the hens fed 18 % CP diet. Increasing dietary CP up to 18 % accompanied with the best (P> 0.01) feed conversion, egg weight and egg mass. The combination between low-CP diet...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Layers; Low-protein diet; Amino acids; Production; Egg quality; Blood.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100712
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Physiological parameters of Piaractus mesopotamicus (Osteichthyes: Characidae) exposed to a biopesticide based on Bacillus thuringiensis Anais da ABC (AABC)
MARIANO,WAGNER S.; AZEVEDO,SAULO B.; GOMES,FRANCISCO L.; LIMA,LIANA B.D.; MORON,SANDRO E.; TAVARES-DIAS,MARCOS.
Abstract: This study investigated the physiological effects of a pesticide based on Bacillus thuringiensis (Dipel-WP®) added in the water and diet of Piaractus mesopotamicus during 24 and 48 h. It was added 0.13 g of de B. thuringiensis per kg of commercial feed; and for the fish subjected to the biopesticide in the water of the tanks, it was added 0.13 g/L of the biopesticide. Plasma levels of sodium, chloride, potassium, cholesterol, glucose, triglycerides, cortisol, total protein, alanine aminotransferase (ALT), aspartate aminotransferase (AST), hematocrit, hemoglobin, erythrocytes number, mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), number of thrombocytes and leukocytes were determined. Cortisol, lactate, glucose,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biochemistry; Blood; Farm fish; Freshwater fish.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300612
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The influence of probiotic additives or multienzyme composition on blood biochemical parameters and milk quality of Lithuanian Black-and-White cattle Arq. Bras. Med. Vet. Zootec.
Dailidavičienė,J.; Budreckienė,R.; Gružauskas,R.; Kerzienė,S.; Andrulevičiūtė,V.; Sinkevičienė,I..
ABSTRACT The aim of the study was to determine the effect of Saccharomyces cerevisiae, multienzyme composition supplementation on milk yield, quality, blood biochemical parameters of Lithuanian-Black-and-White cows. For this reason 28 cows were divided into four groups (three experimental and one control) each with seven cows fed balanced ration (control group) and following experimental groups: addition of 40g supplement of live yeast with organic selenium (group A); 40g supplement of live yeast with aromatic additives (group B); and 0.2g supplement of multienzyme composition (group C) during a 90 days period. The study showed that milk yield was 2.64%, 1.75%, 1.4% higher in groups A, B, C respectively, comparing with the control group. The milk SCC in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dairy cow; Probiotic; Yeast; Milk yield; Blood.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000300939
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Growth and hematological responses of tambaqui fed different amounts of cassava (Manihot esculenta) Arq. Bras. Med. Vet. Zootec.
Aride,P.H.R.; Oliveira,A.T.; Oliveira,A.M.; Ferreira,M.S.; Baptista,R.B.; Santos,S.M.; Pantoja-Lima,J..
ABSTRACT Tambaqui,Colossoma macropomum is a fish of primary importance in Brazilian aquaculture and in the Amazon region in particular. The aim of this work is to analyze the combined effects of physical training and levels of dietary cassava (Manihot esculenta) on the hematological parameters, food intake, conversion efficiency, growth ratio and swimming performance of this fish. A diet for tambaqui consisting of 30% cassava caused decreases in weight gain and specific growth rate compared with the control group. Diets containing 15% or 45% cassava did not affect these indices and did not cause hematological changes in tambaqui juveniles, indicating that some amount of cassava can be used as an alternative energy source for this important aquaculture...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Teleosts; Growth; Feed evaluation; Stress; Blood.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000601697
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Effect of a peel passion fruit flour (Passiflora edulis f. flavicarpa) extract on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells BABT
Rebello,Bernardo Machado; Moreno,Silvana Ramos Farias; Ribeiro,Camila Godinho; Neves,Rosane de Figueiredo; Fonseca,Adenilson de Souza da; Caldas,Luis Querino de Araújo; Bernardo-Filho,Mario; Medeiros,Aldo da Cunha.
Passiflora edulis f. flavicarpa (maracuja) is a fruit consumed in Brazil and worldwide. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The effect of P. flavicarpa extract on the radiolabeling of blood constituents and on red blood cells morphology was evaluated. Blood samples from Wistar rats was incubated with P. flavicarpa extract. After that, the labeling of blood constituents with 99mTc was carried out. Samples of plasma and blood cells were precipitated with trichloroacetic acid to isolate the soluble and insoluble fractions of plasma and blood cells. The radioactivity in each fractions was counted and the percentage of radioactivity was determined. Blood smears were also prepared to morphological evaluation and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Labeling; Blood; Passiflora edulis f. flavicarpa; Radiobiocomplexes; Technetium-99m.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600019
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Light microscope observation of circulating human lymphocytes cultured in vitro BABT
Oliveira,Naila Francis Paulo de; Dolder,Mary Anne Heidi; Genari,Selma Candelária.
The purpose of this work was to study the isolation and a light microscopy technique for cultured lymphocytes. Blood samples were obtained by venipuncture with an anticoagulant added and centrifuged in a Percoll density gradient to separate the leukocytes. Lymphocytes were placed in 25 cm ³ tissue culture flasks at 37ºC. After culturing, they were fixed and stained with the methods used for blood smears. Results showed that not all fixing solutions and stains were an equally good choice for cultured lymphocytes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood; In vitro; Lymphocytes; Microscopy; Morphology; Percoll.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500013
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Protective effect of sodium selenite on genotoxicity to human whole blood cultures induced by aflatoxin B1 BABT
Geyikoglu,Fatime; Türkez,Hasan.
The aim of this study was to investigate the effects of selenium and aflatoxin on human whole blood cultures (WBC) in relation to induction of sister-chromatid exchange (SCE). Results showed that the frequency of SCEs in peripheral lymphocytes was significantly increased by the direct-acting mutagen AFB1 (at doses 5 and 10 µM except for 1µM) compared with controls. When sodium selenite (Na2SeO3) was added at a molar ratio of 5x10-7 and 1x10-6, cells did not show significant increase in SCE frequency. Whereas, SCE rates induced by the various AFB1 concentrations could be significantly reduced by the presence of Na2SeO3 in a clear dose-related manner. These results indicated that selenite and AFB1 mutually antagonized their ability to cause DNA damage...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin B1; Selenium; Sister-chromatid exchanges; Genotoxicity; Blood.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800006
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Effect of an extract of Centella asiatica on the biodistribution of sodium pertechnetate (Na99mTcO4) and on the fixation of radioactivity on blood constituents BABT
Diniz,Claudia Leite; Carmo,Fernanda Santos do; Almeida,Danielle Santos de; Santos-Filho,Sebastião David; Missailidis,Sotiris; Fonseca,Adenilson de Souza da; Bernardo Filho,Mario.
This study evaluates the effects of an acute treatment with a Centella asiatica (CA) extract on the biodistribution of the radiopharmaceutical Na99mTcO4 and on the fixation of technetium-99m on blood constituents. Wistar rats were treated with CA extract and, 1 hour after, Na99mTcO4 was administered; organs/tissues were withdrawn and weighted. The radioactivity was counted to calculate the percentage of activity per gram (%ATI/g). Also, blood samples were withdrawn, plasma (P), blood cells (BC), insoluble fraction (IF) and soluble fractions of P and BC were isolated and the radioactivity was counted to calculate the percentage of activity (%ATI). Data indicated that the acute treatment with CA extract changed significantly (p<0.05) the %ATI/g in several...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodistribution; Centella asiatica; Blood; Plasma; Technetium-99m.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700035
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Effects of fenoprofen on the labeling of blood constituents with technetium-99m, the morphology of red blood cells and the plasmid BABT
Pereira,Marcia de Oliveira; Rocha,Gabrielle de Souza; Lombardi,Simone dos Santos; Geller,Mauro; Pereira,Mário José; Santos-Filho,Sebastião David; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
The aim of this work was to evaluate the effect of fenoprofen on the labeling of blood constituents with technetium-99m, on the morphology of red blood cells and on the plasmid DNA. Blood samples from Wistar rats were incubated with fenoprofen and the assay of labeling of blood constituents with technetium-99m (99mTc) was performed. Blood cells, plasma, soluble and insoluble fractions of blood cells and plasma were separated. The radioactivity in each fraction was counted and percentage of incorporated radioactivity (%ATI) was determined. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of the red blood cells (RBC) was evaluated. Plasmid (pBSK) was incubated with fenoprofen with stannous chloride, and agarose gel...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Technetium-99m; Blood; Morphology; Plasmid; Fenoprofen.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700022
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Intraerythrocytic organic phosphates and hemoglobins of skua - Catharacta maccormicki (Stercoraridae): at two different stages of the year in relation to Antartic migration BABT
Landini,Gustavo Fraga; Di Vito Neto,Alfredo; Schwantes,Arno Rudi; Schwantes,Maria Luiza Barcellos; Santos,Marcelo dos.
Catharacta maccormicki blood samples were collected in the winter (October) and in the summer (February) in order to study the intraerythrocytic organic phosphates, hemoglobin (Hb) electrophoretic patterns, oxygen blood equilibrium and stripped Hbs, as well as the effect of 2,3-biphosphoglycerate (BPG) and inositol hexaphosphate (IHP) on oxygen affinity. All the samples (five from the winter and five from the summer) showed the same electrophoretic pattern: one minor fast component and one major slow one. No differences in oxygen affinity and Bohr effect in the samples collected in the winter and in the summer were found. Oxygen affinity was higher in the stripped Hb than in the blood. BPG seemed to have no effect on the functional properties of skua Hb...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Birds; Blood; Electrophoresis; Cold; Affinity; Oxygen.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400010
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