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Intake and digestibility of diets containing energy sources associated with sunflower meal or urea in feedlot steers - doi: 10.4025/actascianimsci.v33i2.11049 Animal Sciences
Cleef, Eric Haydt Castello Branco Van; Unesp, Jaboticabal; Ezequiel, Jane Maria Bertocco; Unesp, Jaboticabal; Fontes, Nivia Araujo; UNESP; Faturi, Cristian; UFRA, Belém; Oliveira, Priscila Neubern; UFSCAR; Stiaque, Marcelo Gil; UNESP.
The aim of this study was to evaluate the intake and total and partial digestibility of diets using corn, soybean hulls and citrus pulp associated with sunflower meal or urea in feedlot steers. Four steers ½ Aberdeen Angus vs. ½ Nellore, fitted with rumen and duodenum cannulas, with 18 months old and 360 kg of initial weight. The diets were composed of two energy sources (corn and citrus pulp) associated with two nitrogen sources (urea and sunflower meal), in a total of four treatments, with forage:concentrate ratio of 40:60. The statistical design was a Latin square with four treatments and four periods of 21 days each. The average daily dry matter intake was influenced (p < 0.05) by the diets, with the highest value was observed in corn and sunflower...
Tipo: Fieldwork; laboratory analysis Palavras-chave: Bovine; Co-products; Digestion; Energy sources; Nitrogen sources 5.04.03.00-1 Bovinos; Coprodutos; Digestão; Fontes energéticas; Fontes nitrogenadas.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/11049
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Changes in hematological biomarkers of Nellore cows at different reproductive stages Animal Sciences
Oliveira, Wagner Dias Coelho de; Dias e Silva, Tairon Pannunzio; Araújo, Marcos Jácome de; Edvan, Ricardo Loiola; Oliveira, Ronaldo Lopes; Bezerra, Leílson Rocha.
This study was conducted to evaluate changes in hematological biomarkers of Nellore cows at different reproductive stages. Fifteen multiparous cows with 4 ± 1 years of age and live weight of 400 ± 50 kg were used at different stages (non-pregnant, in the initial, middle and late pregnancy, at birth, one day postpartum, 30 and 60 days postpartum). Blood collections were performed every 30 days and assayed for the following hematological biomarkers: hemogram (red blood cells, globular volume, hemoglobin, mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC)) and leukogram (total and differential counts). The erythrogram was influenced (p < 0.001) by the reproductive stages, with variation in the results obtained for the total...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood; Bovine; Erythrogram; Leukogram; Postpartum; Prepartum..
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/45725
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Histopatology of the reproductive tract of Nellore pubertal heifers with genital ureaplasmosis Anais da ABC (AABC)
PÔRTO,REGIANI; OLIVEIRA FILHO,BENEDITO; FERRAZ,HENRIQUE; CAIXETA,LUCIANO; VIU,MARCO ANTONIO; GAMBARINI,MARIA LÚCIA.
ABSTRACT In order to study and characterize the lesions in the reproductive tract of Nellore heifers naturally infected with Ureaplasma diversum and presenting granular vulvovaginitis syndrome (GVS), fragments of uterine tube, uterus, cervix, vagina and vulva of 20 animals were evaluated. The macroscopic lesions of the vulvovaginal mucosa were classified in scores of “1” mild, until “4”, severe inflammation and pustular or necrotic lesions. The histopathological evaluation was performed using scores of “1” to “4”, according to the inflammatory alterations. The fragments with severe microscopic lesions (3 and 4) were from the uterine tubes and uterus, which showed leukocytes infiltration and destruction and/or necrosis of epithelium. Alterations in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Tissue evaluation; Reproduction; Ureaplasma diversum.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602987
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Effect of concentration and exposure period to butyrolactone I on meiosis progression in bovine oocytes Arq. Bras. Med. Vet. Zootec.
Adona,P.R.; Leal,C.L.V..
The effect of concentration and exposure period of bovine oocytes to butyrolactone I (BLI) on meiotic block and in vitro maturation (IVM) kinetics was studied. In experiment 1, all oocytes were at germinal vesicle stage (GV), after 6h in culture with 0, 50 and 100µM BLI. After 12h, all oocytes cultured with 50 and 100µM BLI remained in GV. After 24h, less oocytes were in GV with 50µM (82%) than with 100µM BLI (99%, P<0.05). In experiment 2, after 6h IVM, 93% of control oocytes (IVM only) were in GV, while treated oocytes (100µM BLI for 6, 12 or 24h prior to IVM) showed less oocytes in GV with increased exposure period to BLI prior to IVM (83 and 73%, for 6h and 12h, P<0.05). For a 24h inhibition, GV rates were similar to 12h (70%, P>0.05). After...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Meiosis; Oocyte; Butyrolactone I; Kinetics; Nuclear maturation.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300011
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Oocyte activation and preimplantation development of bovine embryos obtained by specific inhibition of cyclin-dependent kinases Arq. Bras. Med. Vet. Zootec.
Perecin,F.; Méo,S.C.; Leal,C.L.V.; Garcia,J.M..
The efficiency of bohemine and roscovitine in combination with ionomycin on parthenogenetic activation and initial embryonic development of bovine oocytes was studied. Two experiments were performed: in the first, different concentrations (0, 50, 75 or 100µM) and different exposure periods (2, 4 or 6 hours) to bohemine or roscovitine were tested for activation rates of in vitro matured (IVM) bovine oocytes, which were pre-exposed to ionomycin. The best treatments, 75µM bohemine and 50µM roscovitine, both for 6h, were used in the second experiment, in which IVM bovine oocytes were exposed to ionomycin, followed or not by bohemine or roscovitine treatment, and evaluated for nuclear status, activation rate and blastocyst development were assessed. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Artificial activation; Bohemine; Roscovitine; Parthenogenesis.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200002
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Polymorphisms of the coding region of Slc11a1 (Nramp1) gene associated to natural resistance against bovine brucellosis Arq. Bras. Med. Vet. Zootec.
Paixão,T.A.; Martinez,R.; Santos,R.L..
Brucelose bovina causada por Brucella abortus é uma importante doença zoonótica, caracterizada pela ocorrência de aborto durante o último trimestre da gestação, o que resulta em diminuição da fertilidade da produção de leite em vacas. A identificação de genes associados à resistência natural contra brucelose tem sido investigada com o objetivo de selecionar animais resistentes à doença. Em bovinos, é controversa a resistência natural contra B. abortus associada ao polimorfismo da região 3' UTR do gene Slc11A1 (Nramp1). Polimorfismos localizados na sequência codificadora de Slc11A1 têm sido identificados em bovinos, contudo a influência sobre a resistência natural contra brucelose não é conhecida. No presente estudo, três novos polimorfismos do gene Slc11A1...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Bovine; Brucellosis; Natural resistance; Nramp1; Slc11a1.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400041
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Cryotop and development of vitrified immature bovine oocytes Arq. Bras. Med. Vet. Zootec.
Hajarian,H; Wahid,H; Rosnina,Y; Daliri,M; Dashtizad,M; Karamishabankareh,H; Abas Mazni,O.
The effectiveness of different cryodevices (open-pulled straw (OPS), electron microscopy grid (EMG), and Cryotop was evaluated for vitrification of immature bovine oocytes. Polar body, metaphase II stage (MII), survivability, and subsequent developmental rates were determined. Only oocytes with four or five layers of cumulus cells were used. Oocytes were equilibrated in two vitrification solutions - 1: 10% DMSO + 10% ethylene glycol (EG) for 30-45sec and 2: 20% DMSO + 20% EG +0.5M sucrose for 25sec -, mounted on one of the cryodevices and directly plunged into liquid nitrogen for 10 days. Immature vitrified oocytes using Cryotop showed the highest rates of polar body extrusion (PB) and nuclear maturity (MII); 41 and 58% respectively. Vitrified oocytes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Immature oocyte; Vitrification; Cryodevice.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000100011
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Uveitis due to anaplasmosis in a newborn Holstein calf Arq. Bras. Med. Vet. Zootec.
Laus,J.L.; Ortiz,J.P.D.; Vicenti,F.A.M.; Brito,F.L.C.; Wehrle,L.M.; Castro Netto,A.; Lisbão,C.B.S..
Relata-se o caso de um bovino com, aproximadamente, seis horas de vida, apresentando histórico de febre, apatia, inapetência e desconforto ocular. No exame oftálmico rotineiro, encontraram-se valores do teste da lágrima de Schirmer aumentados, diminuição da pressão intra-ocular, hemorragia conjuntival, uveíte anterior, edema corneal e injeção ciliar em ambos os olhos. Coletou-se amostra de sangue para realização de esfregaço sangüíneo, hemograma e bioquímica sérica. No esfregaço visibilizaram-se corpúsculos de Anaplasma marginale em hemácias parasitadas. Quanto à bioquímica sérica, não foram observadas alterações. Os mesmos exames foram realizados na mãe do animal tendo como resultado a positividade para A. marginale. Para o controle da uveíte foi...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Holstein; Uveitis; Anaplasmosis.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600038
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Development and evaluation of accessories to improve the posture of veterinary surgeons in surgical procedures conducted in the field Arq. Bras. Med. Vet. Zootec.
Vulcani,V.A.S.; Silva,L.A.F.; Rabelo,R.E.; Silva,O.C.; Mendes,L.A.; Freitas,S.L.R.; Rabbers,A.S.; Assis,B.M..
ABSTRACT The study was conducted based on the information collected on rural properties in the state of Goiás, during practical classes of Surgical Clinic in Large Animals at the Veterinary Hospital of the Escola de Veterinária e Zootecnia of the Universidade Federal de Goiás and during the implementation of outreach projects developed by the institution. An acropostite-phimosis surgical procedure in the bulls in the field was selected, lasting over 30 minutes and requiring movements, posture and strength on the part of the surgeon. Devices were proposed and developed to provide improved comfort and safety to surgeons. The first device was a stool to be used by the professional during the execution of the surgical intervention. The use enabled the surgeon...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Surgery; Diseases; Postoperative.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501301
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Hematological and immunological development from birth to six months of age in Holstein calves Arq. Bras. Med. Vet. Zootec.
Baccili,C.C.; Oliveira,S.M.F.N. de; Costa,J.F.R; Massoco,C.O.; Pozzi,C.R.; Gomes,V..
ABSTRACT The hematological and immunological development of calves from birth to 6 months of age was performed by hemogram and cellular phenotype. Ten male Holstein calves were assessed in 13 moments: before colostrum intake (D0), every 2 days until the 10th day of life (D2 to D10), at the 15th day after birth (D15), and then monthly up to 6 months (D180). Calves presented hemoconcentration on day (D) 0 and showed a gradual decrease in hematimetric rates until D180. The inversion of the neutrophilic for lymphocytic profile was observed on D4. The percentage of CD3+ cells on D10 was higher than D30 up to D180. The number of CD4+ and CD8+ cells did not change between time points. The number of CD21+ lymphocytes was significantly higher at early time points...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Immune system; Leukogram; Immunophenotyping; Flow cytometry.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000601823
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Bovine placentome preservation for light microscopy evaluation Arq. Bras. Med. Vet. Zootec.
Martins,E.; Martins,V.M.V.; Marques Júnior,A.P.; Vasconcelos,A.C.; Chiarini-Garcia,H.; Malard,P.F..
This work evaluates the preservation of bovine placentomes using paraformaldehyde and glutaraldehyde at 4% and solutions of Karnovsky, Bouin and Carnoy for 4, 12 and 24 hours fixation, at room temperature or at 4ºC, before inclusion in plastic resin and paraffin. The best preservation of the specimens was obtained after 12 hours of fixation with those solutions containing aldehydes. With Bouin solution the best time of fixation was 4 hours, while for Carnoy solution the best time of fixation was 24 hours. Inclusion in plastic resin gave better results than in paraffin, and the temperature did not influence the quality of preservation of the specimens of bovine placentomes for evaluation with light microscopy.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Placentome; Light microscopy.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000200006
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Clinical and pathological presentation of squamous metaplasia of the rete ovarii in a Zebu cow Arq. Bras. Med. Vet. Zootec.
Borges,Á.M.; Peixoto,D.G.M.; Paixão,T.A.; Santos,R.L..
Squamous metaplasia of rete ovarii is characterized by replacement of the normal cuboidal epithelium of rete ovarii by a keratinized stratified scamous epithelium, leading to accumulation of keratinized material within the tubules and cystic dilatation of rete ovarii. The present study decribes a case of scamous metaplasia of rete ovarii in a 10 year old Zebu cow, including clinical, surgical, ultrasonographic, histopathological and hormonal findings. At first ultrasound examination the cow had lightly asymmetric ovaries with follicles presenting echogenicity similar to luteinized follicular cysts. After transvaginal follicular aspiration creamy yellowish sanguineous-purulent content was recovered. After unilateral ovariectomy the ovary was sectioned and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Epidermoid cyst; Ovary; Rete ovarii; Teratoma.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000100017
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Effect of Rolipram on in vitro maturation, gene expression and embryonic development in bovines Arq. Bras. Med. Vet. Zootec.
Santana,B.B.; Sobral,G.G.; Gomes,E.T.; Batista,A.M.; Teixeira,L.P.R.; Tavares,K.C.S.; Bertolini,M.; Carneiro,G.F..
ABSTRACT The aim of this work was to evaluate the effect of the Rolipram during the maturation of bovine oocytes and gene expression of embryos produced in vitro. Bovine ovaries were collected in slaughterhouse. The COCs were selected and divided into 5 groups: Control 0 time; Control: IVM for 24 hours; Rolipram treatments with IVM blocking for 24 hours in maturation medium containing (100, 150 and 200µM). After 24 hours all groups were reseated in IVM for another 24 hours. Subsequently COCs were subjected to the same IVM system and fertilized, being checked for cleavage post fertilization and for blastocyst. In addition, performed expression of the following genes: Mater, BMP15 and Bax. No difference was found in gene expression. Of oocytes evaluated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Rolipram; IVF; IVM; Oocyte.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000501433
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Occurrence of Mycobacterium avium subsp. paratuberculosis in coalho cheese in the State of Pernambuco, Brazil Arq. Bras. Med. Vet. Zootec.
Albuquerque,P.P.F.; Cezar,R.D.S.; Pinheiro Junior,J.W.; Grazielle Nascimento,G.; Santos,A.S.; Mota,R.A..
ABSTRACT Paratuberculosis is a chronic and incurable disease that affects ruminants and other domestic animals. It is caused by Mycobacterium avium subsp. paratuberculosis (MAP) that may also be involved in some human diseases such as Crohn's disease, type 1 diabetes, sarcoidosis, multiple sclerosis, and Hashimoto's thyroiditis. The objective of this study was to investigate the occurrence of MAP DNA in samples of artisanal coalho cheese purchased in the State of Pernambuco. Forty samples of coalho cheese submitted to the Real Time Polymerase Chain Reaction (qPCR) technique were analyzed for the detection of the MAP region IS900. 11 (27.5%) were positive with a mean of 195.9 MAP colony forming unit (CFU) per gram of each sample, with a minimum of 30.3...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; IS900; QPCR; Paratuberculosis.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000601917
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Molecular characterization of Malassezia sympodialis and Malassezia furfur from cattle with and without otitis Arq. Bras. Med. Vet. Zootec.
Duarte,E.R.; Hamdan,J.S..
A molecular study of Malassezia strains isolated from cattle with or without otitis was carried out by random amplified polymorphic DNA analysis (RAPD). DNA was extracted and purified from nine strains of Malassezia sympodialis and fourteen of Malassezia furfur. These microorganisms were collected from eight different bovine herds in Minas Gerais state, Brazil. The RAPD analysis and phenograms did not show the formation of genetically distinct groups among the strain isolated from cattle with or without otitis raised in the same herds. Genetic heterogeneity was observed among Malassezia strains from different geographic origins. These data suggest that genetically similar M. sympodialis and M. furfur strains found as members of the normal ear microbiota...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Otitis; Malassezia spp.; RAPD.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000400001
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Impaired protamination and sperm DNA damage in a Nellore bull with high percentages of morphological sperm defects in comparison to normospermic bulls Arq. Bras. Med. Vet. Zootec.
Carreira,J.T.; Trevizan,J.T.; Kipper,B.H.; Perri,S.H.V.; Carvalho,I.R.; Rodrigues,L.H.; Silva,C.; Koivisto,M.B..
The routine semen evaluation assessing sperm concentration, motility and morphology, does not identify subtle defects in sperm chromatin architecture. Bulls appear to have stable chromatin, with low levels of DNA fragmentation. However, the nature of fragmentation and its impact on fertility remain unclear and there are no detailed reports characterizing the DNA organization and damage in this species. The intensive genetic selection, the use of artificial insemination and in vitro embryo production associated to the cryopreservation process can contribute to the chromatin damage and highlights the importance of sperm DNA integrity for the success of these technologies. Frozen-thawed semen samples from three ejaculates from a Nellore bull showed high...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Bovine; Chromatin integrity; Protamine; Sperm morphology.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200417
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Bovine demodicosis: a case report Arq. Bras. Med. Vet. Zootec.
Lemos,L.S.; Santos,A.S.O.; Sales,L.G.; Silveira,L.S.; Carvalho,E.C.Q..
Tissues samples collected at necropsy from a 9 year-old Holstein cow were fixed in buffered 10% formalin for histological examination. Macroscopic finding did not reveal typical nodular cutaneous lesions. Vulvar microscopic sections of cutaneous tegument showed mites (Demodex bovis), alone and grouped in hair follicles and sebaceous glands.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Demodicosis; Parasitosis; Dermatopathology.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600004
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Evaluation of muscle tissue and liver glycogen of cattle submitted to transport over long distances and subjected to emergency slaughter Arq. Bras. Med. Vet. Zootec.
Burns,L.V.; Ramos,A.T.; Veiga,A.P.M.; Moron,S.E.; Cordova,F.M.; Miotto,F.R.C.; Viana,E.B.; Zimermann,F.C.; Minharro,S.; Stefanine,N.R.; Sousa,L.F..
ABSTRACT The study evaluated the effect of transportation over long distances on cattle muscle tissue of submitted to emergency slaughter in slaughterhouses in northern Tocantins, Brazil. The evaluations consisted in pH, muscle and liver glycogen, muscle histopathology and creatine kinase (CK), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity. Animals were placed into two groups: Experimental Group (EG), consisting of 19 bovines intended for immediate emergency slaughter, and Control Group (CG), composed of 24 bovines slaughtered in accordance with the normal flow. CK and ALT levels were high in EG. AST did not differ between groups. EG showed higher muscle pH and mean of degenerate fibers, mainly on the intercostal. However,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Muscle degeneration; Muscle glycogen; Stress; PH.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000301067
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Dominant follicle removal by ultrasound guided transvaginal aspiration and superovulatory response in Nellore cows Arq. Bras. Med. Vet. Zootec.
Gradela,A.; Esper,C.R.; Matos,S.P.M.; Lanza,J.A.; Deragon,L.A.G.; Malheiros,R.M..
The present study analyzed the physiological significance of the dominant follicle (DF) and its removal on the superovulatory response of 42 Nellore cows and tested the hypothesis that the number of subordinate small follicles (3-8mm) could be used as a single criterion for the evaluation of the presence or absence of a DF in the ovaries. Ultrasonographic examinations were performed two days before the beginning of superovulatory treatment (D0) and the cows divided into three groups: group without DF (NDF), group with a DF (DF) and group with an aspirated DF (ADF). The superovulatory response of the donors did not differ between groups, but the rate of embryonic viability was higher in NDF (69.40%) and ADF (68.99%) groups compared to DF (48.54%). The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Nellore; Dominant follicle aspiration; Superovulatory response.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000100013
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Interferon tau secretion in cattle embryos in vitro fertilized before and after cryopreservation Arq. Bras. Med. Vet. Zootec.
Araújo,M.C.C.; Vale Filho,V.R.; Ferreira,A.M.; Sá,W.F.; Barreto Filho,J.B.; Camargo,L.S.A.; Serapião,R.V.; Silva,M.V.G.B..
The effect of cryopreservation in IFN-tau, from bovine embryos produced in vitro was evaluated. Two treated groups (G1= fresh bovine embryos, n=59 and G2= freezed embryos, n=84) were used to study the effect of cryopreservation on IFN-tau secretion. After reaching the blastocyst phase, the embryos were kept on individual culture for additional period of 7 days. On days 3 and 7 after the beginning of embryos cultivation, samples of the media culture were taken for IFN-tau secretion titration. Oocysts taken from follicles ranging from 3 to 5mm in diameter were obtained from ovaries of females at slaughterhouse. The embryos were frozen, after being dehydrated with ethylene glycol (1.8m), conditioned on 0.5ml palletes and frozen. Frozen embryos secreted lower...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Embryo; PIV; Interferon-tau.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600008
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