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| Registros recuperados: 29 | |
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| Castro, George Meredite Cunha de; Benevides, Norma Maria Barros; Cabral, Maulori Curié; Miranda, Rafael de Souza; Gomes Filho, Enéas; Rocha, Maria Valderez Ponte; Araújo, Marjory Lima Holanda. |
| The seaweeds are bio-resource rich in sulfated and neutral polysaccharides. The tropical seaweed species used in this study (Solieria filiformis), after dried, shows 65.8% (w/w) carbohydrate, 9.6% (w/w) protein, 1.7% (w/w) lipid, 7.0% (w/w) moisture and 15.9% (w/w) ash. The dried seaweed was easily hydrolyzed under mild conditions (0.5 M sulfuric acid, 20 min.), generating fermentable monosaccharides with a maximum hydrolysis efficiency of 63.21%. Galactose and glucose present in the hydrolyzed were simultaneously fermented by Saccharomyces cerevisiae when the yeast was acclimated to galactose and cultivated in broth containing only galactose. The kinetic parameters of the fermentation of the seaweed hydrolyzed were Y(P⁄S) = 0.48 ± 0.02 g.g−1, PP = 0.27 ±... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ciências da Vida Bioquímica iota-carrageenan; Cellulose; Galactose; Kinetic parameters; Fermentation; Acclimation; Sulfuric acid. Biotecnologia. |
| Ano: 2017 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/37227 |
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| Han,Lirong; Feng,Juntao; Zhang,Shuangxi; Ma,Zhiqing; Wang,Yonghong; Zhang,Xing. |
| The efficiency of enzymatic hydrolysis of cellulose can be improved by various pretreatments of the substrate. In order to increase the efficiency of enzymatic saccharification of the wheat straw, we determined the effect of different pretreatments on the physical structure, chemical components and enzymatic saccharification of wheat straw. Our results showed that combination of grinding and sodium hydroxide (NaOH) treatment had high effect on the enzymatic hydrolysis of wheat straws. The optimal pretreatment condition was to grind the wheat straws into the sizes of 120 meshes followed by treatment with 1.0% NaOH for 1.5 h (121°C/15psi). Under this condition, the cellulose content of wheat straw was increased by 44.52%, while the content of hemicellulose... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellulase; Cellulose; Fermentation; Hydrolysis; Lignocellulosic; Pretreatment. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100006 |
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| Cunha-Santino,Marcela B. da; Bianchini Jr.,Irineu. |
| Decomposition of lignocellulosic detritus at enzymatic level is the rate-limiting step during aquatic macrophyte decomposition. Assays were carried out to evaluate cellulase activity during decomposition of Utricularia breviscapa; the assays included the following C-sources: leachate, lignocellulosic matrix and integral detritus. The incubations comprised U. breviscapa C-sources added to Óleo lagoon (21º 36'S and 47º 49'W) water maintained in the dark under anaerobic condition at 15ºC, 20ºC, 25ºC and 30ºC. Cellulase activity were monitored during 180 days, with the activity showing a time evolution that depended on the temperature and type of detritus: (i) cellulase activity tended to occur earlier with increasing temperatures, but it was less intense than... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellulase; Utricularia breviscapa; Cellulose. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200008 |
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| Gréggio,Thiago Claudino; Assis,Luiz Carlos; Nahas,Ely. |
| The decomposition of soil litter contributes to maintaining agricultural sustainability, since the nutrients released by microbial activity are determinants of the net productivity of the agroecosystem. The decomposition of rubber tree (Hevea brasiliensis [Willd. ex A. Juss.] Müll. Arg.) leaves located on the surface and buried in at 10 cm depth in Forest and Savannah (Cerradão) soils (Oxisols) was studied, with emphasis on the production of CO2 and the monthly variation of the remaining amounts of litter mass, soluble substances, cellulose and lignin. To evaluate CO2 production, H. brasiliensis leaves were incubated for 30 days in soils from the 0-2 cm and 10-12 cm layers. CO2 production increased in the 0-2 cm layer in comparison to the 10-12 cm layer.... |
| Tipo: Journal article |
Palavras-chave: Litter mass; Cellulose; Lignin; Soluble substances; Litter-bags. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000200002 |
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| Bai,Xi; Yuan,Xianjun; Wen,Aiyou; Li,Junfeng; Bai,Yunfeng; Shao,Tao. |
| Background: Cold-active endo-1, 4-β-glucanase (EglC) can decrease energy costs and prevent product denaturation in biotechnological processes. However, the nature EglC from C. farmeri A1 showed very low activity (800 U/L). In an attempt to increase its expression level, C. farmeri EglC was expressed in Escherichia coli as an N-terminal fusion to protein S (ProS) from Myxococcus xanthus. Results: A novel expression vector, pET(ProS-EglC), was successfully constructed for the expression of C. farmeri EglC in E. coli. SDS-PAGE showed that the recombinant protein (ProS-EglC) was approximately 60 kDa. The activity of ProS-EglC was 12,400 U/L, which was considerably higher than that of the nature EglC (800 U/L). ProS-EglC was active at pH 6.5-pH 8.0,... |
| Tipo: Journal article |
Palavras-chave: Cellulose degradation; Cellulose; Cold-active enzyme; Endoglucanases; Enzymatic properties; Escherichia coli; Expression; Novel expression vector; N-terminal fusion; Protein S-tag; Recombinant protein. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600012 |
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| Wang,Nengfei; Zang,Jiaye; Ming,Kaili; Liu,Yu; Wu,Zuohao; Ding,Hui. |
| Background: Cellulose can be converted to ethanol by simultaneous saccharification and fermentation (SSF). The difference between the optimal temperature of cellulase and microbial fermentation, however, has been identified as the critical problem with SSF. In this study, one fungal strain (AnsX1) with high cellulase activity at low temperature was isolated from Antarctic soils and identified as Verticillium sp. by morphological and molecular analyses. Results: The biochemical properties of crude AnsX1 cellulase samples were studied by filter paper cellulase assay. The maximum cellulase activity was achieved at low temperature in an acidic environment with addition of metal ions. Furthermore, AnsX1 cellulase demonstrated 54-63% enzymatic activity at... |
| Tipo: Journal article |
Palavras-chave: Antarctic soil; Cellulose; Filter paper cellulase assay; Psychrotrophic fungi; Verticillium sp. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400010 |
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| Valenzuela,Eduardo; Hipp,Joseline; Alonso,Claudia; Godoy,Roberto; Alberdi,Miren; Alvarez,Maricel; Saavedra,Isabel. |
| At present, reforestation has focused on native forests with anthropogenic intervention and eroded soils. There is interest in producing Nothofagus seedlings which can overcome adverse conditions encountered on reforestation sites. It is necessary to find new fungi that can be utilized as mycorrhizal inoculants and that enable the seedlings to increase their tolerance to adverse conditions. Two ectomycorrhizal strains of the fungus Descolea antarctica (D1 and D2) were cultured at different temperatures, pH levels and the activities of amylases, cellulases, and phosphatases were determined. In greenhouse and nursery trials, the growth responses of inoculated Nothofagus obliqua seedlings were evaluated. D1 and D2 exhibited the highest growth rates at 23ºC.... |
| Tipo: Journal article |
Palavras-chave: Cellulose; Ectomycorrhizal fungi; Nothofagus; PH; Phosphatase; Temperature. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200012 |
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| Rasmussen-Poblete,Susana; Valdes,Jorge; Gamboa,Maria Cecilia; Valenzuela,Pablo D.T; Krauskopf,Erwin. |
| Eucalyptus globulus is the most important commercial temperate hardwood in the world because of its wood properties and due to its characteristics for biofuel production. However, only a very low number of expressed sequence tags (ESTs) are publicly available for this tree species. We constructed a cDNA from E. globulus seedlings subjected to low temperature and sequenced 9,913 randomly selected clones, generating 8,737 curated ESTs. The assembly produced 1,062 contigs and 3,879 singletons forming a Eucalyptus unigene set. Based on BLASTX analysis, 89.3% of the contigs and 88.5% of the singletons had significant similarity to known genes in the non-redundant database of GenBank. The Eucalyptus unigene set generated is a valuable public resource that... |
| Tipo: Journal article |
Palavras-chave: Cellulose; Cold-temperature; EST database; Forest biotechnology; Lignin. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200008 |
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| MELO, C. F. M. de; SOUZA, H. B. de; LOUREIRO, M. R. C.; WISNIEWSKI, A.. |
| Reunindo excelentes características, a madeira de Ucuúba (Virola surínamensis (Rol) Warb) tem sido extensivamente empregada na fabricação de laminados. Como consequêncía do processo produtivo, a madeira que não pode ser integralmente aproveitada, deixa um apreciável resíduo que, a falta de melhor emprêgo, não tem tido outra utilizaçãc senão como combustível na própria Usina. Estudando-se a ucuúba como fonte de celulose para papel, conclui-se que se trata, na verdade, de excelente matéria: prima, mesmo os resíduos de laminação que se constituem de restos de madeira de várias idades; Podem ser produzidos papéis Kraft de bôa qualidade, em ambos os casos, embora quando se trate da utilização dos resíduos de laminação as características de resistência ao... |
| Tipo: Folhetos |
Palavras-chave: Analysis; Fiber; Análise; Celulose; Fibra; Madeira; Papel; Ucuuba; Tecnologia; Virola Surinamensis; Cellulose; Kraft paper; Technology; Wood. |
| Ano: 1971 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/376076 |
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| Registros recuperados: 29 | |
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