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Efecto de quitina y quitosano sobre huevos y juveniles de nemátodos formadores de nódulos radiculares, Nacobbus aberrans y Meloidogyne incognita bajo condiciones de in vitro e in vivo Colegio de Postgraduados
Sánchez Portillo, Juan Fernando.
El nematodo agallador Meloidogyne incognita Kofoid y White, 1919, Chitwood, 1949, distribuido en 23 de los 32 estados del país y Nacobbus aberrans Thorne 1935, Thorne and Allen, 1944, distribuido en 10 de 32 estados de la República Mexicana, su combate para ambas especies, casi siempre se hace aplicando diferentes agroquímicos, los cuales algunos ya tienen problemas de registro. Se necesita evaluar nuevos productos que sean otra alternativa a su manejo y mas amigables al ambiente. En el presente trabajo se evaluó el efecto de quitina-quitosano con diferentes grados de desacetilación, biopolímeros naturales que poseen propiedades nematicidas y utilizados como reguladores de la población de estos fitonematodos; los estudios se hicieron in vitro, sobre la...
Palavras-chave: Biopolímero natural; Meloidogyne incognita; Nacobbus aberrans; Desacetilación; Control; Quitina; Quitosano; Chitin; Chitinase; Natural biopolymer; Chitosan; Deacetylation; Fitopatología; Maestría.
Ano: 2010 URL: http://hdl.handle.net/10521/55
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Efecto de quitina y quitosano sobre huevos y juveniles de nemátodos formadores de nódulos radiculares, Nacobbus aberrans y Meloidogyne incognita bajo condiciones de in vitro e in vivo Colegio de Postgraduados
Sánchez Portillo, Juan Fernando.
El nematodo agallador Meloidogyne incognita Kofoid y White, 1919, Chitwood, 1949, distribuido en 23 de los 32 estados del país y Nacobbus aberrans Thorne 1935, Thorne and Allen, 1944, distribuido en 10 de 32 estados de la República Mexicana, su combate para ambas especies, casi siempre se hace aplicando diferentes agroquímicos, los cuales algunos ya tienen problemas de registro. Se necesita evaluar nuevos productos que sean otra alternativa a su manejo y mas amigables al ambiente. En el presente trabajo se evaluó el efecto de quitina-quitosano con diferentes grados de desacetilación, biopolímeros naturales que poseen propiedades nematicidas y utilizados como reguladores de la población de estos fitonematodos; los estudios se hicieron in vitro, sobre la...
Palavras-chave: Biopolímero natural; Meloidogyne incognita; Nacobbus aberrans; Desacetilación; Control; Quitina; Quitosano; Chitin; Chitinase; Natural biopolymer; Chitosan; Deacetylation; Fitopatología; Maestría.
Ano: 2010 URL: http://hdl.handle.net/10521/55
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Effect of edible quinoa protein-chitosan based films on refrigerated strawberry (Fragaria × ananassa) quality Electron. J. Biotechnol.
Valenzuela,Carolina; Tapia,Cristian; López,Luis; Bunger,Andrea; Escalona,Victor; Abugoch,Lilian.
Background Strawberries are non-climacteric fruits with a low respiration rate, but are subject to serious fungal deterioration during postharvest handling. The edible coatings based on chitosan (CH), quinoa protein-chitosan (Q/CH) and quinoa protein-chitosan-sunflower oil (Q/CH/SO) may provide a solution to this problem. Thus, in this work CH, Q/CH and Q/CH/SO were elaborated and applied to fresh strawberries, and its effect on the strawberries shelf life during storage for 15 d was evaluated by mold and yeast count, fungal decay, carbon dioxide rate, physicochemical properties, and sensory evaluation. Results On all analysis days, the strawberries coated with the film-forming CH, Q/CH and Q/CH/SO solutions presented a significant lower amount of mold and...
Tipo: Journal article Palavras-chave: Chitosan; Edible films; Quinoa protein; Strawberry; Sunflower oil.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600004
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Characterization of a Gelatin/Chitosan/Hyaluronan scaffold-polymer Electron. J. Biotechnol.
Enrione,Javier; Osorio,Fernando; López,Daniel; Weinstein-Oppenheimer,Caroline; Fuentes,Miguel A; Ceriani,Ricardo; Brown,Donald I; Albornoz,Fernando; Sánchez,Elizabeth; Villalobos,Patricio; Somoza,Rodrigo A; Young,Manuel E; Acevedo,Cristian A.
Gelatin, chitosan and hyaluronic acid are natural components used to prepare polymeric scaffold in tissue engineering. The physical properties of these materials confer an appropriate microenvironment for cells, which can be used as a regeneration system for skin and cartilage. In this work, we prepared and characterized a Gelatin/Chitosan/Hyaluronan lyophilized-polymer. Physical properties of lyophilized-polymer changed slightly with moisture, but when polymer was totally hydrated the elasticity changed significantly. Thermophysical characterisation indicated that temperatures higher than 30ºC could modify irreversibly the polymeric matrix probably due to protein denaturation. Besides, we used the polymer as scaffold to prepare a biosynthetic-skin,...
Tipo: Journal article Palavras-chave: Chitosan; Fibrin; Gelatin; Hyaluronic acid; Scaffold.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500020
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Growth of Cunninghamella elegans UCP 542 and production of chitin and chitosan using yam bean medium Electron. J. Biotechnol.
Stamford,Thayza Christina Montenegro; Stamford,Tânia Lucia Montenegro; Stamford,Newton Pereira; Barros Neto,Benicio de; Campos-Takaki,Galba Maria de.
Microbiological processes were used for chitin and chitosan productions by Cunninghamella elegans (UCP 542) grown in a new economic culture medium. The assay was carried out to evaluate the growth of C. elegans using yam bean (Pachyrhizus erosus L. Urban) medium, in different times of growth (24, 48, 72 and 96 hrs), incubated at 28ºC in an orbital shaker at 150 rpm. The lyophilized biomass was determined by gravimetry. The polysaccharides were extracted by alkali-acid treatment, and characterized by infrared spectroscopy, titration and viscosity. C. elegans grown in the yam bean medium and produced higher yields of biomass (24.3 g/ mL) in 96 hrs. The high level was chitosan (66 mg/g), and chitin (440 mg/g) were produced at 48 and 72 hrs of growth,...
Tipo: Journal article Palavras-chave: Biopolymers; Chitin; Chitosan.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100006
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Design Polysaccharides of Marine Origin: Chemical Modifications to Reach Advanced Versatile Compounds ArchiMer
Chopin, Nathalie; Guillory, Xavier; Weiss, Pierre; Le Bideau, Jean; Colliec-jouault, Sylvia.
Polysaccharides are among the most abundant macromolecules on Earth. These polymers are easily obtained from various marine resources such as algae, microorganisms and crustacean shells. The structure of these natural carbohydrates is innovative and quite complex. Marine biopolymers represent key scaffolds toward large challenging fields, such as biomedical applications (glycosaminoglycans, regenerative medicine and drug delivery) and tailored biomaterials. Chemical modifications can be applied to modify their final properties in a specific purpose. New functional glycans are achievable and represent a real potential with their intrinsic biocompatibility and biodegradability. Hydroxyl groups are ubiquitous in polysaccharides structure and involved in most...
Tipo: Text Palavras-chave: Alginates; Carrageenans; Chemical modification; Chitin; Chitosan; Grafting; Marine polysaccharide; Polymer.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00199/31027/29902.pdf
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Lactic acid bacteria selection for biopreservation as a part of hurdle technology approach applied on seafood ArchiMer
Wiernasz, Norman; Cornet, Josiane; Cardinal, Mireille; Pilet, Marie-france; Passerini, Delphine; Leroi, Francoise.
As fragile food commodities, microbial and organoleptic qualities of fishery and seafood can quickly deteriorate. In this context, microbial quality and security improvement during the whole food processing chain (from catch to plate), using hurdle technology, a combination of mild preserving technologies such as biopreservation, modified atmosphere packaging and superchilling, are of great interest. Thirty-five lactic acid bacteria (LAB) belonging to the species Carnobacterium maltaromaticum, Carnobacterium inhibens, Lactococcus piscium, Leuconostoc gelidum, Vagococcus fluvialis, Vagococcus penaei and Aerococcus viridans, known to possess interesting antimicrobial activity, were chosen for their potential application as bioprotective agents as a part of...
Tipo: Text Palavras-chave: Antimicrobial activity; Spoilage; Screening; Chitosan; Fish juice; Safety assessment.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00382/49342/49732.pdf
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Preparation and in vitro characterization of chitosan nanoparticles containing Mesobuthus eupeus scorpion venom as an antigen delivery system J. Venom. Anim. Toxins incl. Trop. Dis.
Mohammadpour Dounighi,N; Eskandari,R; Avadi,MR; Zolfagharian,H; Mir Mohammad Sadeghi,A; Rezayat,M.
Hydrophilic nanoparticles have been widely investigated in recent years as delivery systems for therapeutic macromolecules such as antigens. In the present study Mesobuthus eupeus venom-loaded chitosan nanoparticles were prepared via ionic gelation of tripolyphosphate (TPP) and chitosan. The optimum encapsulation efficiency (91.1%) and loading capacity (76.3%) were obtained by a chitosan concentration of 2 mg/mL, chitosan-to-TPP mass ratio of 2 and M. eupeus venom concentration of 500 µg/mL. The average nanoparticle size at optimum conditions was determined by Zetasizer (Malvern Instruments, UK). The nanoparticle size was about 370 nm (polydispersity index: 0.429) while the zeta potential was positive. Transmission electron microscope (TEM) imaging showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nanoparticle; Chitosan; Tripolyphosphate; Venom; Antigen delivery system; Mesobuthus eupeus.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100006
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Películas y cubiertas de quitosana en la conservación de vegetales OceanDocs
García, M. A..
El uso de películas y cubiertas de quitosana en vegetales constituye un método alternativo para su conservación y contribuye, además, a minimizar la contaminación ambiental debida a los desechos generados por el empleo de envases no biodegradables y el vertimiento a los mares de los desechos de la industria de mariscos. Se discuten algunas propiedades y aplicaciones de este biopolímero en la industria alimentaria.
Tipo: Journal Contribution Palavras-chave: Shelf life; Reviews (literature); Vegetables; Chitosan.
Ano: 2008 URL: http://hdl.handle.net/1834/4843
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Envasado activo de embutidos OceanDocs
García, M.A.; Díaz, R.; Puerta, F.; Beldarraín, T.; Castillo, A.; González, J.; Duarte, C..
El objetivo del estudio fue evaluar la influencia de coberturas de quitosana en lascas de jamón cocido y jamón Visking envasadas al vacío. En ambos productos se observó un incremento de la acidez, mayor en los productos con coberturas de quitosana. Se produjo un aumento de la dureza con el tiempo, sin que existieran diferencias significativas (p≤0,05) atribuibles a la aplicación de coberturas. Se encontró un efecto favorable de las coberturas de quitosana en cuanto a retardar el desarrollo de los psicrófilos. La calidad sensorial fue el parámetro que más se afectó durante el almacenamiento; en el jamón cocido el sabor se tornó ácido y se observó un exudado lechoso y en el jamón Visking los jueces refirieron olores y sabores atípicos y una disminución...
Tipo: Journal Contribution Palavras-chave: Conservation; Cold storage; Packaging materials; Sausages; Hams; Chitosan; Wrapping.
Ano: 2008 URL: http://hdl.handle.net/1834/4892
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Preservación de pimientos con aplicación de coberturas de quitosana OceanDocs
García, M.; Díaz, R.; Casariego, A.; Bolumen, S.; de Hombre, R.; González, I.; Rodríguez, I.; Salgado, J. M..
Se aplicaron coberturas de quitosana en pimientos (Capsicum annuum L.) con el objetivo de evaluar su influen- cia en el mejoramiento de la calidad y prolongación del perío- do de conservación. Cada lote de pimientos fue tratado en forma diferente, considerando muestras sin tratar y tratadas con disoluciones de quitosana. Las coberturas se aplicaron mediante pinceladas con disoluciones a 1 y 2 % (m/v) de quitosana en ácido láctico a 1 %. Posterior al secado de los frutos, estos se envasaron en bolsas de polietileno de baja densidad. Se determinaron varios parámetros de calidad du- rante el almacenamiento de 10 a 12 ºC: pérdida de peso, textu- ra, sólidos solubles, pH y evaluación sensorial mediante el análisis descriptivo cuantitativo. Las coberturas de...
Tipo: Journal Contribution Palavras-chave: Preservation (organisms); Green peppers; Bell peppers; Chitosan.
Ano: 2009 URL: http://hdl.handle.net/1834/5059
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Modelación de las propiedades mecánicas de películas de quitosana OceanDocs
Casariego, A.; Díaz, R.; Sergrañes, Y.; Nieto, O. M.; Durán, C.; López, N; Rodríguez, M.; González, J..
Se elaboraron películas a partir de disoluciones a 3 % (m/v) de quitosana en ácido láctico a 1% (v/v), empleando glicerina, sorbitol o polietilenglicol como plastificante en concentraciones de 0,25 a 0,50 mL/g de quitosana. Se midieron el esfuerzo tensil, elongación, fuerza de ruptura, deformación y el espesor de las películas, con el objetivo de evaluar la influencia de la masa molar y concentración de plastificante sobre sus propiedades mecánicas. Se obtuvieron los modelos matemáticos que relacionan estos factores. Las películas con polietilenglicol, presentaron mayor esfuerzo tensil y fuerza de ruptura normalizada que las plastificadas con glicerina o sorbitol, pero resultaron menos elásticas y deformables, además, mientras mayor fue la concentración de...
Tipo: Journal Contribution Palavras-chave: Mechanical properties; Chitosan; Biopolymers; Elasticity.
Ano: 2009 URL: http://hdl.handle.net/1834/5095
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Physiology and postharvest conservation of ‘Paluma’ guava under coatings using Jack fruit seed-based starch Rev. Bras. Frutic.
Rodrigues,Antonio Augusto Marques; Silva,Silvanda de Melo; Dantas,Ana Lima; Silva,Antonio Fernando da; Santos,Leonardo da Silva; Moreira,Dayse das Neves.
Abstract The aim of this work was to evaluate the effect of jackfruit seed starch-based (S) coatings, added to chitosan and alginate on the physiology and maintenance of quality of cold stored ‘Paluma’ guavas, followed by transfer to the room condition. The design was the completely randomized, in a 4x2 factorial scheme, in 4 replications, with 4 coatings (dispersion of S - 4%; S 2% + chitosan - 2% (SC); S - 2% + alginate - 2% (SA); and the uncoated control), in 2 environments (refrigerated (10±2 °C e 80±2% RH) with transfer to room condition (25±3 °C e 75±4% HR)), on the 16th and 20th day of cold storage. The SC and SA coatings were efficient in reducing the respiratory rate in fruits during 10 days at room condition. The SC coating delayed fruit...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artocarpus heterophyllus; Fruit waste; Chitosan; Sodium alginate; Fruit quality.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000200303
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Effect of Chitosan Coating on Some Quality Properties of Thomson Orange during Storage (A case study in Iran) CIGR Journal
Taghinezhad, Ebrahim; Rasooli Sharabiani, Vali.
 Most of the world's consumers have used the natural coatings instead of chemical coatings. In this research, some quality properties of Thomson orange were evaluated using Chitosan edible coating (at concentrations of 0.5, 1 and 2%) and chemical coating of fungicide (Ortho Phenyl Phenol) as compared to uncoated samples during storage for three months. Fruit were maintained in cold storage at 6 °C temperature and 85-90% relative humidity, then pH, total soluble solid (TSS) of orange juice and percentage of fruit weight loss were measured during storage every month. The results showed that weight loss of fruit with 2% chitosan coating was lower than other treatments. Also this treatment had high resistance against fungal attacks due to high values of pH, so...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitosan; Citrus; Fungicide; Quality; Storage.
Ano: 2018 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/4189
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Synthesis of calcium-phosphate and chitosan bioceramics for bone regeneration Anais da ABC (AABC)
FINISIE,MELLATIE R.; JOSUÉ,ATCHE; FÁVERE,VALFREDO T.; LARANJEIRA,MAURO C. M..
Bioceramic composites were obtained from chitosan and hydroxyapatite pastes synthesized at physiological temperature according to two different syntheses approaches. Usual analytical techniques (X-ray diffraction analysis, Fourier transformed infrared spectroscopy, Thermo gravimetric analysis, Scanning electron microscopy, X-ray dispersive energy analysis and Porosimetry) were employed to characterize the resulting material. The aim of this investigation was to study the bioceramic properties of the pastes with non-decaying behavior from chitosan-hydroxyapatite composites. Chitosan, which also forms a water-insoluble gel in the presence of calcium ions, and has been reported to have pharmacologically beneficial effects on osteoconductivity, was added to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioceramic; Chitosan; Hydroxyapatite; Composites; Bone regeneration.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652001000400006
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Antibacterial activity of chitosan biofilm for the conservation of fertile and table eggs Arq. Bras. Med. Vet. Zootec.
Prado,R.O.F.; Tellez,I.G.; García,M.L.J.; Aldaco,L.L.E.; García,C.A.C..
ABSTRACT The aim of this study was to develop a chitosan biofilm against Salmonella enteritidis, for the conservation of fertile and table eggs. Two experiments were performed. Experiment 1: 400 specific pathogen-free table eggs were divided in a completely randomized design into four treatments, five replicates and each replicate with 20 table eggs. Experimental groups were assigned to control and 1, 5 and 10% chitosan treatment. The eggs were immersed in the chitosan solution. They were then exposed to Salmonella enteritidis and stored for 1, 24, 96 and 168h at 4ºC. The eggs were then washed with 10mL of physiological saline solution. Experiment 2: 80 specific pathogen-free fertile eggs were tested, the assays were assigned to control and 1, 5 and 10%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitosan; Fertile eggs; Table eggs; Salmonella enteritidis.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100208
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Greenhouse and field assessment of different organic compounds against guava-parasitic Meloidogyne enterolobii Bragantia
Almeida,Alexandre Macedo; Souza,Ricardo Moreira; Gomes,Vicente Martins; Miranda,Guilherme Bessa.
Guava decline is a complex disease involving Meloidogyne enterolobii and Fusarium solani and it has caused major direct losses to Brazilian growers. Although several strategies have been sought to control the nematode, the use of organic soil amendments is currently the best approach to manage this disease. To assess the best amount of meat and bone meal (MBM) to be incorporated into the soil, guava seedlings inoculated with M. enterolobii were treated with 1-5% v/v of the MBM. Ninety days later variables related to nematode reproduction and plant development were evaluated, which indicated a potential nematicidal effect of the MBM at 3%. Another experiment assessed nematode- and plant-related variables 90 days after treatment of the seedlings with MBM,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Psidium guajava; Fusarium solani; Neem cake; Chitosan; Shrimp shell; Cultural control; Guava decline; Meat and bone meal.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052012000100011
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Soybean β-Glucosidase immobilisated on chitosan beads and its application in soy drink increase the aglycones BABT
Grade,Luciana Carvalho; Moreira,Amanda Aleixo; Varea,Geni da Silva; Mandarino,José Marcos Gontijo; Silva,Josemeyre Bonifácio da; Ida,Elza Iouko; Ribeiro,Mara Lúcia Luiz.
The objective of this study was to investigate the immobilisation efficiency of soybean β-glucosidase (181.6 U/mL; 23.8 mg protein/mL) on activated chitosan beads. Central Composite Rotational Design (CCDR) 23 was used and the application of immobilised enzyme in commercial soy drink was evaluated. The activation of chitosan beads was achieved with established 2.5% glutaraldehyde, pH 7.5, 8 h incubation time (6 h with agitation and 2 h without agitation) at 37ºC. The highest immobilisation efficiency (%) of soybean β-glucosidase on chitosan beads obtained was 37.74 U/mL and 18.84 mg protein/4 chitosan beads at pH 7.5 and 20 h coupling time of enzyme-matrix (7 h with agitation and 13 h without agitation) at 4ºC. The immobilised enzyme incubated at 50ºC, pH...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Β-glucosidase soybean; Immobilisation; Chitosan; Aglycones.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500766
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Antibacterial activity of chitosan and the interpolyelectrolyte complexes of poly(acrylic acid)-chitosan BABT
Ortega-Ortiz,Hortensia; Gutiérrez-Rodríguez,Baltazar; Cadenas-Pliego,Gregorio; Jimenez,Luis Ibarra.
The antimicrobial activity of chitosan and water soluble interpolyelectrolyte complexes of poly(acrylic acid)-chitosan was studied. Chitosans of two different molecular weights were tested at different concentration for 0.5 to 5 g·L-1 as antimicrobial agents against P. aeruginosa and P. oleovorans. In both cases, the best microbial inhibition was obtained with the concentration of 5 g·L-1. However, the interpolyelectrolyte complexes of poly(acrylic acid)-chitosan with composition φ =2 produced higher antibacterial activity than the two chitosans at the concentration of 0.5 g·L-1. The NPEC2 complex was more effective than chitosans. This could be attributed to the number of moles of the amino groups of chitosan and the carboxylic acid groups of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibacterial activity; Chitosan; Complexes; Poly(acrylic acid); Polyelectrolytes.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300016
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Effects of Emerita brasiliensis flour supplementation on normotensive (Wistar) and spontaneously hypertensive rats (SHR) BABT
Cavargere,Victor Agati; França,Camille Feitoza; Cardoso,Ricardo; Vianna,Lucia Marques.
Several studies characterize the connection of overweight and obesity with chronic diseases. Therefore, new alternatives are being studied for controlling hypertension, such as chitin and chitosan fibers, commonly found on crustacean's carapace like Emerita brasiliensis. Rats from two different strains were divided into control and supplemented groups (n=6). The Wistar strain experiment started with a 14-days baseline period, followed by supplementation of E.brasiliensis flour added to the diet in the doses of 5, 10 and 20%, for a 14-days period each. With the optimal dose of 20%, the study was undertaken with SHR rats, starting with a 7-days baseline period, followed by three weeks of supplementation. Data were evaluated using one-way ANOVA and p<0.05...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitin; Chitosan; Emerita brasiliensis; Blood pressure.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400006
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