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| Registros recuperados: 34 | |
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| Pomilio,Alicia Beatriz; Battista,Miguel Edgardo; Vitale,Arturo Alberto; Battista,Stella Maris; Ricciardi,Armando Ítalo Amadeo; Torres,Ana María; Camargo,Francisco José. |
| En el presente trabajo se examinó la interacción de las amanitinas de Amanita phalloides (Basidiomycetes) con los venenos de las serpientes Bothrops neuwiedi diporus ("yarará pequeña"), B. alternatus ("yarará grande"), Crotalus durissus terrificus ("serpiente de cascabel") y de la abeja mielera Apis mellifera. Se aplicaron las técnicas de Ouchterlony, inmunotransferencia, electroforesis rocket y electroforesis en gel de poliacrilamida a los anti-venenos y anti-toxinas obtenidos por inmunización en caballos y/o en conejos. Los antisueros de serpientes y las amanitinas reaccionaron en forma cruzada, así como el veneno de abeja y las amanitinas. Cuando los venenos de Bothrops neuwiedii diporus y Crotalus durissus terrificus se preincubaron con las amanitinas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amanita phalloides; Apis mellifera mellifera; Bothrops neuwiedi diporus; Bothrops alternatus; Crotalus durissus terrificus; Reactividad cruzada; Interacción; Electroforesis en gel de poliacrilamida-dodecil sulfato de sodio; Tiempo de coagulación humano. |
| Ano: 2012 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572012000200002 |
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| Santos,K.R.; Takahira,R.K.; Rall,V.L.M.; Calderón,C.; Sequeira,J.L.; Silva,R.J.. |
| This study reported the pulmonary, microbiological, and hematological alterations in Crotalus durissus terrificus parasitized by nematodes of the genus Rhabdias. Histological, microbiological, and hematological analysis were performed on parasitized (n=6) and non-parasitized (n=6) snakes. Granulocytic and mononuclear cell infiltrates in the pulmonary parenchyma and epithelium were also observed during the histological analysis of parasitized snakes. Microbiological analysis of parasitized animals revealed the following Gram-negative bacteria: Citrobacter divergens, Burkholderia cepacia, Stenotrophomonas maltophilia, Proteus vulgaris, Enterobacter sakazakii, Enterobacter ammnigenus, Pseudomonas aeruginosa, Pantoea sp. and Providencia rettgeri. In... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake; Crotalus durissus terrificus; Rhabdias spp.; Histopathology; Microbiology; Hematology. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300022 |
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| ARAÚJO,Terezinha; MORO,Luciana; LÚCIA,Maria; GOLLOUBEFF,Bárbara; VASCONCELOS,Anilton César. |
| Com o objetivo de estudar a fauna parasitológica dos ofídios do serpentário da UNIFENAS - Universidade de Alfenas, MG, foram necropsiados alguns espécimes de cascavéis (Crotalus durissus terrificus) e urutus (Bothrops alternatus), mantidos em cativeiro. Esses animais apresentavam anorexia, desidratação, diarréia, anemia e morte em torno de dois meses após o início dos sinais clínicos. Foram identificados helmintos dos gêneros Kalicephalus, Ophidascaris, Rhabdias e Oxyuris; protozoários do gênero Haemogregarina e acarinos do gênero Ophionysus. Aos sobreviventes foi administrado tratamento específico com Ivermectin, bem como terapia auxiliar à base de vitamina A e complexo B. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crotalus durissus terrificus; Nematoda; Protozoários; Ácaro. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000100003 |
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| We describe the isolation of crotoxin, a presynaptic B-neurotoxin, as well as its subunits B (crotactine) and A (crotapotin) from lyophilized Crotalus durissus terrificus venom by a single-step preparative isoelectric focusing procedure. From 98 mg of dried venom protein 20.1 mg of crotactine and 13.1 mg of crotapotin were recovered in the first step of focalization and 4.2 mg in a second run. These values correspond to 35.7% of the total venom protein applied. Crotactine separated in the 9.3-7.0 pH range (tubes 1-6) and crotapotin in the 1.8-2.8 pH range (tubes 15-19) and both were homogeneous by SDS-PAGE and N-terminal amino acid analysis. Crotactine, a 12-kDa protein, presented hemolytic and phospholipase A2 activity. Thus, using isoelectric focusing we... |
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Palavras-chave: Crotalus durissus terrificus; Crotactine; Crotapotin; Phospholipase A2; Isoelectrofocusing; Presynaptic B-neurotoxin. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100004 |
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| SILVA,R. J. DA; FECCHIO,D.; BARRAVIERA,B.. |
| The effect of Crotalus durissus terrificus (LAURENTI, 1768) venom on the evolution of Ehrlich ascites tumor cells was evaluated. Thus, 30-day-old male mice of the Swiss strain were inoculated intraperitoneally with 1x10<img SRC="http:/img/fbpe/jvat/v3n2/image1947.gif"> tumor cells. Then, 7 groups of animals were formed: 3 control groups (physiological, venom and tumor) and 4 experimental groups that received different doses of venom. The experimental groups received 5 intraperitoneal venom injections on the 1<img SRC="http:/img/fbpe/jvat/v3n2/image1948.gif"> , 4<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"> , 7<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"> , 10<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"> and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Venom; Inflammation; Tumor growth; Crotalus durissus terrificus. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301997000200008 |
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| BARRAVIERA,B.; SARTORI,A.; PEREIRA DA SILVA,M. F.; KANENO,R.; PERAÇOLI,M. T. S.. |
| A sandwich-type ELISA technique for specific and sensitive detection of Crotalus durissus terrificus venom antigens, horse-antivenom, human IgG and IgM antibodies was set up. Sixteen patients, 13 males and 3 females aged between 13 to 63 years (mean 33 ± 15) bitten by Crotalus durissus terrificus snakes were studied. Of the 15 patients, 6 had previously received anti-Crotalus venom and no seric venom was detected. For the other 9 patients studied, the venom levels ranged from 2 to 108 ng/ml according to the severity of each case. Seric antivenom was detected up to 44 days after the bite. IgM human antibody levels against Crotalus venom were higher between 3 and 18 days after specific treatment. IgG human antibody levels against Crotalus venom were detected... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzyme-linked immunosorbent assay; Crotalus durissus terrificus; Venom; Antivenom; Antibodies; Cerebrospinal fluid. |
| Ano: 1996 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301996000100003 |
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| FONSECA,M. G.; MATHIAS,M. R. C.; YAMASHITA,S.; MORCELI,J.; BARRAVIERA,B.. |
| The objective of this study was to detect the presence of local edema and hemorrhage caused by Crotalus durissus terrificus envenoming using magnetic resonance imaging (MRI). Six patients bitten by Crotalus durissus terrificus snakes were treated at the Emergency Unit and Tropical Diseases Unit of Botucatu School of Medicine, UNESP, São Paulo State, Brazil. After receiving specific serotherapy, the patients were submitted to MR of the bite site. Post contrast T1 and T2 spin-echo MRI were obtained revealing the following lesions: edema associated with hemorrhage in subcutaneous and muscular tissue (n=3), edema in subcutaneous tissue (n=2), and perimuscular hemorrhage (n=1). In this study, MR demonstrated a local effect of Crotalus durissus terrificus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crotalus durissus terrificus; Muscular damage; Edema; Hemorrhage; Magnetic resonance. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100005 |
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| SILVA,R. J. DA; FONTES,M. R. M.; RODRIGUES,R. R.; BRUDER,E. M.; STEIN,M. F. B.; SIPOLI,G. P. M.; PINHÃO,R.; LOPES,C. A. DE M.. |
| This report documents a case of a melanic specimen of Crotalus durissus terrificus (Laurenti, 1768) found in Bofete, São Paulo State, Brazil. The authors describe this melanic snake, determine the electrophoretic pattern of its venom, and compare the venom of this specimen against that of normal Crotalus durissus terrificus. This report is very important because melanism is a rare chromatic anomaly |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Chromatic anomalies; Melanism; Crotalus durissus terrificus; Venom electrophoresis. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301999000100008 |
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| Ferreira Junior,R. S.; Nascimento,N.; Couto,R.; Alves,J. B.; Meira,D. A.; Barraviera,B.. |
| Laboratory profile of young ovines was studied in order to evaluate and compare their antiserum production from natural and Cobalt-60 irradiated Crotalus durissus terrificus (C.d.t.) venoms. The parameters analyzed included complete blood count, and urea, creatinine, aspartate aminotransferase, total proteins, albumin and globulin serum measurements. Three groups of six animals each were used. Group 1 (G1) received natural C.d.t. venom; Group 2 (G2) received irradiated C.d.t. venom; and Group 3 (G3) was used as control and did not receive venom, only adjuvants, using seven venom inoculations. During the experimental period, animals were fortnightly weighed. According to clinical and weight evaluation, sheep in post-weaning phase showed no changes in their... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crotalus durissus terrificus; Hyperimmunization; Ovines; Antivenom; Irradiation. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400009 |
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| Netto,D. P.; Chiacchio,S. B.; Bicudo,P. L.; Alfieri,A. A.; Balarim,M. R. S.; Nascimento,N.. |
| Natural (NV) and Cobalto60-irradiated (IrV) Crotalus durissus terrificus venom were used to evaluate serum production capacity of sheep and possible hematological and biochemical effects. Freeze-dried venom aliquots were diluted in acidified saline solution (NaCl 150 mM, pH 3.0) and irradiated by a Cobalt 60 source at a dose of 5.54 x 102 Gy/h and a concentration of 2.000 Gy. Twelve sheep were divided into two groups of six animals. One group received irradiated venom (IrV) and the other natural venom (NV). Three antigen doses (venom) were administered at monthly intervals. Blood samples were collected weekly for analysis of serum neutralization potency and capacity, complete blood count (CBC), total plasma protein, fibrinogen, albumin, and globulin. At... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hematology; Gamma radiation; Irradiated crotalic venom; Crotalic antiserum; Ovine; Crotalus durissus terrificus. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000100004 |
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| Nogueira,R. M. B.; Sakate,M.; Sangiorgio,F.; Laposy,C. B.; Melero,M.. |
| The present study aimed at evaluating clinical and laboratory aspects during experimental envenomation by Crotalus durissus terrificus in dogs treated with antiophidic serum. Twenty-one dogs were divided into three groups of seven animals each. Group I received 1mg/kg venom (sc); Group II received 1mg/kg venom (sc), 50mg antiophidic serum (iv), and fluid therapy including 0.9% NaCl solution (iv); and Group III received 1mg/kg venom (sc), 50mg antiophidic serum (iv), and fluid therapy including 0.9% NaCl solution containing sodium bicarbonate diluted to the dose of 4mEq/kg. The clinical signs of ataxia, sedation, flaccid paralysis, mydriasis, eyeball paralysis, mandible ptosis, sialorrhea, vomiting and diarrhea observed in the dogs were very similar to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crotalus durissus terrificus; Dogs; Clinical aspects; Laboratory aspects. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400010 |
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| Bordon,Karla C. F.; Wiezel,Gisele A.; Cabral,Hamilton; Arantes,Eliane C.. |
| BackgroundCrotalus durissus terrificus venom (CdtV) is one of the most studied snake venoms in Brazil. Despite presenting several well known proteins, its L-amino acid oxidase (LAAO) has not been studied previously. This study aimed to isolate, characterize and evaluate the enzyme stability of bordonein-L, an LAAO from CdtV.Methods The enzyme was isolated through cation exchange, gel filtration and affinity chromatography, followed by a reversed-phase fast protein liquid chromatography to confirm its purity. Subsequently, its N-terminal amino acid sequence was determined by Edman degradation. The enzyme activity and stability were evaluated by a microplate colorimetric assay and the molecular mass was estimated by SDS-PAGE using periodic acid-Schiff... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crotalus durissus terrificus; L-amino acid oxidase; Rattlesnake; Enzyme activity; Enzyme stability; Chromatography; Snake venom; Yellow venom; Stabilization. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100335 |
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| Registros recuperados: 34 | |
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