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| Registros recuperados: 88 | |
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| Zappelini,Julia; Souza,Luiz Gustavo; Guerra,Miguel Pedro; Pescador,Rosete. |
| ABSTRACT Although karyotype features are useful data for evolutionary studies, cytogenetic data in Bambusoideae are mainly based only on chromosome counts. The first comparative cytogenetic analysis of three Neotropical woody bamboo species - Guadua chacoensis, G. angustifolia and Chusquea tenella - was undertaken based on new and reviewed chromosome counts, CMA/DAPI double staining, fluorescent in situ hybridization (FISH) with 35S and 5S rDNA probes, and genome size estimation. The two species of Guadua were found to have 2n = 46 chromosomes, while the first record for C. tenella was 2n = 44. Only one pair of CMA+/DAPI- was detected on the terminal region of metacentric chromosomes in all three species. Likewise, one pair of 5S and 35S rDNA sites was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chusquea; Cytogenetics; Diploidization; Evolution; Guadua; Polyploidy. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000400673 |
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| Techio, Vânia Helena; UNC; Davide, Lisete Chamma; UFLA; Souza, Tatiana Matioli; 3Bios Consultoria e Serviços Ambientais Ltda; Pereira, Antônio Vander; EMBRAPA-MG. |
| Avaliou-se o complemento cromossômico de 16 acessos de Pennisetum disponíveis na Coleção de Germoplasma da Embrapa Gado de Leite – Juiz de Fora, Estado de Minas Gerais. Para a avaliação citogenética, utilizou-se a técnica de esmagamento e coloração com Feulgen. Para quatro acessos foram obtidas metáfases com 2n = 28 cromossomos, confirmando sua classificação como P. purpureum. A origem híbrida P. purpureum x P. glaucum foi constatada em oito acessos, os quais apresentaram 2n = 21. Em um acesso identificado como híbrido entre P. purpureum x P. glaucum, foram observadas metáfases com 2n = 54, permitindo inferir que não se trata de um híbrido interespecífico triplóide. Para outros três acessos avaliados, também foram observadas células com 2n = 54, embora... |
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Palavras-chave: 2.00.00.00-6 Ciências Biológicas Pennisetum; Citogenética; Germoplasma; Híbridos interespecíficos. Pennisetum; Cytogenetics; Germplasm; Interspecific hybrids. |
| Ano: 2008 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5016 |
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| RAMOS,ROSEMAR S. L.; VALE,WILLIAM G.; ASSIS,FÁTIMA L.. |
| A total of 30 animals of the genus Dasyprocta were cytogenetically studied. They belong to the following species: D. prymnolopha (N=20), D. leporina (N=6), D. fuliginosa (N=1) and Dasyprocta sp. (N=3) (Dasyproctidae, Hystricognathi). Cell suspensions were obtained by peripheral blood culture, besides bone marrow and spleen cells, from D. prymnolopha and D. leporina. The diploid number was 64/65 for all samples. The karyotypes showed similarity, and chromosomal polymorphism was not detected by Giemsa conventional staining and G banding. The constitutive heterochromatin distribution at the pericentromeric region of all the chromosomes was similar in all species. D. prymnolopha, D. leporina and Dasyprocta sp. presented variation in the heterochromatical block... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytogenetics; Hystricognathi; Dasyprocta; Karyotype. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652003000100007 |
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| AMORIM,MARÚCIA I. M.; MERGLER,DONNA; BAHIA,MARCELO O.; DUBEAU,HÉLÈNE; MIRANDA,DANIELA; LEBEL,JEAN; BURBANO,ROMMEL R.; LUCOTTE,MARC. |
| The mercury rejected in the water system, from mining operations and lixiviation of soils after deforestation, is considered to be the main contributors to the contamination of the ecosystem in the Amazon Basin. The objectives of the present study were to examine cytogenetic functions in peripheral lymphocytes within a population living on the banks of the Tapajós River with respect to methylmercury (MeHg) contamination, using hair mercury as a biological indicator of exposure. Our investigation shows a clear relation between methylmercury contamination and cytogenetic damage in lymphocytes at levels well below 50 micrograms/gram, the level at which initial clinical signs and symptoms of mercury poisoning occur. The first apparent biological effect with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brazilian Amazon; Mercury; Mitotic Index; Cytogenetics. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000400004 |
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| Carvalho de Souza,Patrícia; Salim Khayat,André; Chamon Seligmann,Igor; Rodríguez Burbano,Rommel Mario. |
| The collared peccary (Tayassu tajacu) is widely distributed over the American continent, being found from the south of the USA to the north of Argentina. In Brazil, it is spread all over the country, being one of the potential species to be raised in captivity. Therefore, the cytogenetic techniques could be a potential tool for reproductive monitoring of animals raised in captivity, mainly when destined for commercial purposes. This study had the objective of determining the chromosome number of two populations raised in captivity and characterizing them by GTG banding. For this purpose, an analysis was made of mitotic metaphases obtained from lymphocyte cultures made from blood samples of 11 animals, six of which from the Northeast and five from the North... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cytogenetics; GTG Banding; Tayassuidae; Suidae; Pigs. |
| Ano: 2008 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200007 |
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| QUINDERÉ,YEDA R. S. D; LOURENÇO,LUCIANA B; ANDRADE,GILDA V; TOMATIS,CRISTIAN; BALDO,DIEGO; RECCO-PIMENTEL,SHIRLEI M. |
| We investigated the NOR distribution in ten populations of Physalaemus cuvieri from different regions of Brazil and Argentina. A high variability in NOR pattern was observed and provided a useful tool in grouping several populations. The specimens from the state of Tocantins, northern Brazil, could easily be distinguished from all the other analyzed populations, since its karyotype presented NORs in the chromosome pairs 1,3,4 and 10 (and sometimes also in chromosome 5), and several pericentromeric C-bands. A NOR-site in chromosome 9 characterized three populations from the northeastern region of Brazil. Interestingly, the P. cuvieri populations located in opposite extremes of the geographic distribution had, as a fixed condition, the presence of NORs in 8q... |
| Tipo: Journal article |
Palavras-chave: Anura; Chromosome; Cytogenetics; NOR; Physalaemus; Polymorphic variations; Polytypic variations. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100008 |
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| Velloso,E.D.R.P.; Chauffaille,M.L.; Peliçario,L.M.; Tanizawa,R.S.S.; Toledo,S.R.C.; Gaiolla,R.D.; Lopes,L.F.. |
| Myelodysplastic syndromes (MDS) and juvenile myelomonocytic leukemia (JMML) are rare hematopoietic stem cell diseases affecting children. Cytogenetics plays an important role in the diagnosis of these diseases. We report here the experience of the Cytogenetic Subcommittee of the Brazilian Cooperative Group on Pediatric Myelodysplastic Syndromes (BCG-MDS-PED). We analyzed 168 cytogenetic studies performed in 23 different cytogenetic centers; 84 of these studies were performed in patients with confirmed MDS (primary MDS, secondary MDS, JMML, and acute myeloid leukemia/MDS+Down syndrome). Clonal abnormalities were found in 36.9% of the MDS cases and cytogenetic studies were important for the detection of constitutional diseases and for differential diagnosis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Childhood myelodysplastic syndromes; Juvenile myelomonocytic leukemia; Cytogenetics; Karyotype; Diagnosis. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100085 |
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| Santos,M.F.M.; Oliveira,F.C.A.C.; Kishimoto,R.K.; Borri,D.; Santos,F.P.S.; Campregher,P.V.; Silveira,P.A.A.; Hamerschlak,N.; Mangueira,C.L.P.; Duarte,F.B.; Crepaldi,A.H.; Salvino,M.A.; Velloso,E.D.R.P.. |
| Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), myeloproliferative neoplasms (n=137), and other with conclusive diagnosis (n=6), and reactive disorders/no conclusive diagnosis (n=75). The rate of unsuccessful karyotyping was 18.5% and was associated with the use of PB and a low number of nucleated cells... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytogenetics; Karyotype; Leukemia; Myelodysplasia; Myeloproliferative disease. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200608 |
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| Pina-Neto,J.M.; Carrara,R.C.V.; Bisinella,R.; Mazzucatto,L.F.; Martins,M.D.; Sartoratto,E.; Yamasaki,R.. |
| The objective of the present study was to determine the frequency of somatic chromosomal anomalies and Y chromosomal microdeletions (azoospermia factor genes, AZF) in infertile males who seek assisted reproduction. These studies are very important because the assisted reproduction techniques (mainly intracytoplasmic sperm injection) bypass the natural selection process and some classical chromosomal abnormalities, microdeletions of AZF genes or some deleterious genic mutations could pass through generations. These genetic abnormalities can cause in the offspring of these patients male infertility, ambiguous external genitalia, mental retardation, and other birth defects. We studied 165 infertile men whose infertility was attributable to testicular problems... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytogenetics; Y microdeletions; Male infertility; Karyotype; Chromosome aberrations. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400017 |
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| Ribeiro,E.M.S.F.; Rodriguez,J.M.; Cóser,V.M.; Sotero,M.G.; Fonseca Neto,J.M.; Pasquini,R.; Cavalli,I.J.. |
| Microsatellites are short tandem repeat sequences dispersed throughout the genome. Their instability at multiple genetic loci may result from mismatch repair errors and it occurs in hereditary nonpolyposis colorectal cancer. This instability is also found in many sporadic cancers. In order to evaluate the importance of this process in myeloid leukemias, we studied five loci in different chromosomes of 43 patients, 22 with chronic myelocytic leukemia (CML) in the chronic phase, 7 with CML in blast crisis, and 14 with acute myeloid leukemia (AML), by comparing leukemic DNA extracted from bone marrow and constitutional DNA obtained from buccal epithelial cells. Only one of the 43 patients (2.1%), with relapsed AML, showed an alteration in the allele length at... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genomic instability; Mismatch repair errors; Cytogenetics; Leukemogenesis. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200003 |
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| Peres,Wellington Adriano Moreira; Bertollo,Luiz Antonio Carlos; Moreira Filho,Orlando. |
| Characidae is one of the largest fish families of the Neotropical region, and presenting a pronounced morphological variability, certainly does not constitute a monophyletic group. The cytogenetical data also show a large chromosomal variation and can provide important information for a better understanding of the relationships between the species of this group. 18S and 5S rDNA probes were used in the present study for the chromosomal mapping in different Characidae species from the São Francisco River (Astyanax lacustris, Astyanax scabripinnis, Hasemania nana, Piabina argentea, Orthospinus franciscensis, Serrapinnus heterodon, Serrapinnus piaba and Myleus micans) and Alto Paraná (Astyanax altiparanae) basins. Species with a single pair of chromosomes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytogenetics; Neotropical fish; Fluorescent in situ hybridization (FISH); RDNA probe. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200009 |
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| Bellafronte,Elisangela; Schemberger,Michelle Orane; Artoni,Roberto Ferreira; Moreira Filho,Orlando; Vicari,Marcelo Ricardo. |
| Parodontidae fish show few morphological characteristics for the identification of their representatives and chromosomal analyses have provided reliable features for determining the interrelationships in this family. In this study, the chromosomes of Apareiodon hasemani from the São Francisco River basin, Brazil, were analyzed and showed a karyotype with 2n = 54 meta/submetacentric chromosomes, and a ZZ/ZW sex chromosome system. The study revealed active NORs located on pair 11 and additional 18S rDNA sites on pairs 7 and 22. The 5S rDNA locus was found in pair 14. It showed a pericentric inversion regarding the ancestral condition. The satellite DNA pPh2004 was absent in the chromosomes of A. hasemani, a shared condition with most members of Apareiodon.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytogenetics; FISH; 18S rDNA; 5S rDNA; Neotropical fishes. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500010 |
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| Registros recuperados: 88 | |
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