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| Registros recuperados: 179 | |
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| González Jiménez, Vianey. |
| Se llevó a cabo una caracterización morfológica de 12 variedades de caña de azúcar del estado de Tabasco mediante descriptores morfológicos, se identificó la filogenia por medio de secuenciamiento de la región ITS de DNA ribosómico y trnL-F cloroplástico, y se hizo una caracterización molecular usando la técnica AFLP. La descripción fenotípica se llevó a cabo considerando las características de entrenudo, hoja y yema. En la amplificación del DNA ribosómico se utilizaron los iniciadores ITS4 e ITS5. Para la amplificación del cloroplasto se utilizaron los iniciadores universales trnL-F. El procedimiento para la realización de los AFLPs se realizó siguiendo las recomendaciones descritas en el Kit AFLP®Analysis System I y AFLP® Starter Primer Kit. Entre las 12... |
| Tipo: Tesis |
Palavras-chave: Caña de azúcar; AFLPs; DNA; Variabilidad; Maestría; Producción Agroaliemtaria en el Trópico; Sugarcane; Variability. |
| Ano: 2009 |
URL: http://hdl.handle.net/10521/1558 |
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| Zárate Castrejón, José Luis. |
| La senescencia foliar es la última etapa del desarrollo de la hoja, se caracteriza por una serie de cambios fisiológicos, bioquímicos y estructurales. Las hojas pierden gradualmente clorofila, se desorganiza la maquinaria fotosintética y se reciclan los productos de degradación de las macromoléculas hacia otras partes en crecimiento (hojas jóvenes, flores, frutos) u órganos de almacenamiento como el tubérculo en Solanum tuberosum. Se conoce poco acerca de la relación entre la senescencia foliar y proceso del llenado del tubérculo. El objetivo del presente trabajo fue estudiar la senescencia foliar en dos variedades de papa con diferente ciclo biológico y relacionarlo con el llenado del tubérculo. Se monitoreó el amarillamiento foliar en toda la planta a... |
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Palavras-chave: ADN; Cloroplastos; LSU; Rubisco; SDS-PAGE; Cloroplast; DNA; Maestría; Botánica. |
| Ano: 2010 |
URL: http://hdl.handle.net/10521/275 |
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| Pérez Hernández, Set. |
| La roya naranja (Puccinia kuehnii) es una enfermedad fungosa que afecta al cultivo de la caña de azúcar (Saccharum spp. Híbridos). La enfermedad afecta la capacidad fotosintética de la planta y reduce su crecimiento y desarrollo. El objetivo de la presente investigación fue determinar la presencia de la enfermedad de la roya naranja en clones comerciales de caña de azúcar en el sureste de México e identificar molecularmente el agente causal. En áreas de abastecimiento de los ingenios Santa Rosalía, Presidente Benito Juárez, Azuremex, Tabasco; Huixtla, Chiapas; La Joya, Campeche y San Rafael de Pucté, Quintana Roo, se colectaron hojas con síntomas y lesiones características de la roya naranja. Las hojas se cortaron en pequeñas porciones y se colocaron en... |
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Palavras-chave: Puccinia kuehnii; DNA; Saccharum spp.; Iniciadores; Agente causal; Primers; Causal agent; Producción Agroalimentaria en el Trópico; Maestría. |
| Ano: 2014 |
URL: http://hdl.handle.net/10521/2363 |
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| Riaño Pachón, Diego Mauricio; González Estrada, Elizabeth; Alexa, Adrian; Ramírez, Fidel; Vischi Winck, Flavia; Gómez Merino, Fernando, Coord.; Silva Rojas, Hilda Victoria, Coord.; Pérez Rodríguez, Paulino, Coord.. |
| En esta publicación intitulada “Bioinformática: aplicaciones a la genómica y proteómica” se detallan algunos de los avances más sobresalientes de los temas de genómica y proteómica, derivados de un curso internacional sobre el tema, organizado por el Colegio de Postgraduados. Estos avances incluyen aspectos de las dos ciencias ómicas, incluyendo genómica y biología estructural, código R, análisis comparativo y evolución, agrupamiento y minería de datos en R, redes de interacciones entre proteínas y proteómica bioinformática. BIOINFORMATICS : APPLICATIONS TO GENOMICS AND PROTEOMICS. ABSTRACT : In this publication entitled "Bioinformatics: applications to genomics and proteomics" are some of the most salient issues of genomics and proteomics, derived from an... |
| Tipo: Libro |
Palavras-chave: Bioinformática; Proteómica; Genómica; ADN; Proteínas; Modelación; Simulación; Análisis de genómas; Biología estructural; Código R; Análisis comparativo; Minería de datos; Computación aplicada; Bioinformatics; Proteomics; DNA; Proteins; Models; Genomics; Simulation; R Code; Data mining; Computing; Genome analysis. |
| Ano: 2010 |
URL: http://hdl.handle.net/10521/313 |
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| Clevenger, Anthony P; Western Transportation Institute, Montana State University; apclevenger@gmail.com; Sawaya, Michael A; Department of Ecology, Montana State University; mikesawaya@hotmail.com. |
| Intuitively, wildlife crossing structures should enhance the viability of wildlife populations. Previous research has demonstrated that a broad range of species will use crossing structures, however, questions remain as to whether these measures actually provide benefits to populations. To assess this, studies will need to determine the number of individuals using crossings, their sex, and their genetic relationships. Obtaining empirical data demonstrating population-level benefits for some species can be problematic and challenging at best. Molecular techniques now make it possible to identify species, individuals, their sex, and their genetic relatedness from hair samples collected through non-invasive genetic sampling (NGS). We describe efforts to pilot... |
| Tipo: Peer-Reviewed Reports |
Palavras-chave: Banff National Park; DNA; Genetics; Non-invasive; Road ecology; Ursus americanus; Ursus arctos; Wildlife crossing structure. |
| Ano: 2010 |
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| Crouch,Jonathan H.; Vuylsteke,Dirk; Ortiz,Rodomiro. |
| Bananas and plantains (Musa spp.) are the most important tropical fruit crops. They form an integral component of the farming systems in the humid agroecological zones of the tropics. A broad array of applied cell and molecular techniques are increasingly being used worldwide to facilitate and enhance the handling and improvement of plantain and banana germplasm. Tissue culture is used for germplasm exchange, conservation and rapid multiplication, while in vitro seed germination (based on embryo culture or rescue) plays a critical role in generating hybrid plants. DNA marker systems have been developed in Musa to assist germplasm management, selection within the breeding pool or gene introgression from wild species, and for disease diagnosis. Likewise,... |
| Tipo: Journal article |
Palavras-chave: Banana; Improvement; Micropropagation; DNA; Markers; Plantain. |
| Ano: 1998 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000100002 |
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| Li,Zhongai; Li,Jie; Yanhua,Liu; Wang,Zicheng. |
| Background: Analytical techniques such as methylation-sensitive amplification polymorphism and high-performance liquid chromatography were used to detect variation in DNA methylation of mature Chrysanthemum leaves during the floral transition induced by short-day (SD) treatment. Results: For both early- and late-flowering cultivars, the time from the date of planting to the appearance of the capitulum bud and early blooming were significantly shorter than those of the control. The capitulum development of the early-flowering cultivar was significantly accelerated compared to the control, unlike the late-flowering cultivar. The DNA methylation percentage of leaves was significantly altered during flower development. For the early-flowering cultivar, DNA... |
| Tipo: Journal article |
Palavras-chave: Chrysanthemum; DNA; Methylation; Floral transition; Short day. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300009 |
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| Vasconcellos,Luciana Pimentel de Mello Klocker; Tambasco-Talhari,Daniella; Pereira,Andréa Pozzi; Coutinho,Luiz Lehmann; Regitano,Luciana Correia de Almeida. |
| Aberdeen Angus beef cattle from the Brazilian herd were studied genetically using restriction fragment length polymorphism (RFLP) of the kappa-casein - HinfI (CSN3 - HinfI), beta-lactoglobulin - HaeIII (LGB - HaeIII) and growth hormone AluI (GH- AluI) genes, as well as four microsatellites (TEXAN15, CSFM50, BM1224 and BM7160). The RFLP genotypes were determined using the polymerase chain reaction (PCR) followed by digestion with restriction endonucleases and electrophoresis in agarose gels. With the exception of the microsatellite BM7160, which was analyzed in an automatic sequencer, the PCR products were genotyped by silver staining. The allele and genotype frequencies, heterozygosities and gene diversity were estimated. The values for these parameters of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aberdeen Angus; Bovine; DNA; Characterization; RFLP; Microsatellite. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200005 |
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| Ferreira,Francileide Lisboa; Leal-Mesquita,Emygdia Rosa; Santos,Sidney Emanuel Batista dos; Ribeiro-dos-Santos,Ândrea Kely Campos. |
| Five loci (vWA1, F13A1, D12S67, Apo-B and D1S80) were investigated by polyacrylamide gel electrophoresis followed by silver staining in a sample of 177 individuals from the population of São Luís, State of Maranhão, Brazil. A total of 70 different alleles were identified. A statistically significant deviation from the Hardy-Weinberg equilibrium was observed in a single locus (F13A1, p = 0.0075). The average heterozygosity (H) was estimated at 77.7%, the mean number of alleles per locus as 14. The PD (capacity of genotype differentiation at each locus) ranged from 88.9% (vWA1) to 96.7% (F13A1). The combined PE (power of exclusion) of these five loci was 99.8%. In terms of racial admixture (42% European, 39% Indian, and 19% African Black ancestry), São Luís... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amazon region; DNA; Polymorphisms; VNTRs; STRs; Interethnic admixture. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100004 |
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| Moraes-Barros,Nadia de; Morgante,João Stenghel. |
| Museum collections have been widely used as sources of biological samples for molecular biology studies and there are several methodologies and techniques to obtain and analyze DNA from tissues archived in museums, but most of these protocols have been developed for a specific tissue or are commercial kits. We present a simple protocol for extracting and amplifying DNA segments from sloth museum specimens. With this simple protocol we analyzed DNA fragments from 64% of 64 skin samples from three-toed sloths (Bradypus variegatus and Bradypus tridactylus) archived in three different museums: 43 samples from the University of São Paulo Museum of Zoology (Museu de Zoologia da Universidade de São Paulo, MUZUSP) São Paulo, São Paulo, Brazil; 18 samples from the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bradypus; DNA; Dried skins; Museum specimens. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600024 |
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| Presti,Flavia T.; Meyer,Janaína; Antas,Paulo T.Z.; Guedes,Neiva M.R.; Miyaki,Cristina Y.. |
| Molted feather sampling is a useful tool for genetic analyses of endangered species, but it is often very laborious due to the low quality and quantity of the DNA obtained. In the present study we show the parts of feathers that resulted in better yield of DNA. In descending order these were: blood clot outside the umbilicus, umbilicus (without blood clot), tip, inner membrane, and small calamus. Compared to DNA extracted from blood samples, DNA extracted from feathers produced microsatellite alleles of poorer quality and had to be processed immediately after extraction. As expected due to the level of DNA degradation, molecular sexing protocols that result in shorter PCR products were more efficient. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molted feathers; Blood sample; DNA; PCR product; Parrot. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100019 |
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| Maia,Thais Augusta; Vilaça,Sibelle Torres; Silva,Luciana Resende da; Santos,Fabricio Rodrigues; Dantas,Gisele Pires de Mendonça. |
| Abstract This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine heterologous loci from related species were tested. Seven loci were amplified successfully, with five of them being polymorphic. These loci exhibited amplicons ranging from 110 to 254 bp for 132 samples, with 60 from eggshells and 72 from blood or muscle samples. The number of alleles for M. octosetaceus ranged from one to six (mean = 3.71), which is low compared to M. merganser (1-15 alleles), a ‘least concern’ species. Genetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA; Noninvasive samples; Genetic diversity; Endangered birds; Mergus. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500808 |
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| Sakowski,Sebastian; Krasinski,Tadeusz; Waldmajer,Jacek; Sarnik,Joanna; Blasiak,Janusz; Poplawski,Tomasz. |
| Abstract The development of conventional, silicon-based computers has several limitations, including some related to the Heisenberg uncertainty principle and the von Neumann “bottleneck”. Biomolecular computers based on DNA and proteins are largely free of these disadvantages and, along with quantum computers, are reasonable alternatives to their conventional counterparts in some applications. The idea of a DNA computer proposed by Ehud Shapiro’s group at the Weizmann Institute of Science was developed using one restriction enzyme as hardware and DNA fragments (the transition molecules) as software and input/output signals. This computer represented a two-state two-symbol finite automaton that was subsequently extended by using two restriction enzymes. In... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioinformatics; DNA; DNA computer; Restriction enzymes. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500860 |
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| Luís,C.; Cothran,E.Gus; Oom,M.M.. |
| The use of DNA technology for parentage testing is increasing every day. Most laboratories have improved their exclusion probabilities (PE) by the addition of DNA microsatellite loci to standard blood-typing results. The efficacy of each locus depends on the number of alleles detected and their frequencies in the breeds tested. Here we analyzed the usefulness of six microsatellites for routine parentage testing in three Portuguese autochthonous horse breeds: Lusitano, Sorraia and Garrano. The DNA loci analyzed - ASB2, HMS3, HMS7, HTG4, HTG10, and VHL20 - were chosen based upon the polymorphism detected in other breeds with Iberian horse influence. The estimated probability of exclusion of wrongly named parents (PE) was high, with values ranging from 88.5%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellites; DNA; Portuguese horse breeds; Parentage testing. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200003 |
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| Registros recuperados: 179 | |
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