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| Registros recuperados: 28 | |
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| LI,KE; SHU,XUAN; GONG,HUI; CHENG,LIUHANGHANG; DONG,ZEJUN; SHU,SHENYOU. |
| Abstract: DNA methylation is essential for spatiotemporally-regulated gene expression in embryonic development. TBX22 (Chr X: 107667964-107688978) functioning as a transcriptional repressor affects DNA binding, sumoylation, and transcriptional repression associated with X-linked cleft palate. This study aimed to explore the relationship and potential mechanism between TBX22 exon 5 methylation and palatal shelf fusion induced by all-trans retinoic acid (ATRA). We performed DNA methylation profiling, using MethylRAD-seq, after high throughput sequencing of mouse embryos from control (n=9) and ATRA-treated (to induce cleft palate, n=9) C57BL/6J mice at embryonic gestation days(E) 13.5, 14.5 and 16.5. TBX22 exon 5 was hyper-methylated at the CpG site at... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA methylation; Cleft palate; TBX22; CpG site. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300711 |
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| Negrón-Oyarzo,Ignacio; Lara-Vásquez,Ariel; Palacios-García,Ismael; Fuentealba,Pablo; Aboitiz,Francisco. |
| Schizophrenia is a severe psychiatric disorder that results in a significant disability for the patient. The disorder is characterized by impairment of the adaptive orchestration of actions, a cognitive function that is mainly dependent on the prefrontal cortex. This behavioral deficit, together with cellular and neurophysiological alterations in the prefrontal cortex, as well as reduced density of GABAergic cells and aberrant oscillatory activity, all indicate structural and functional deficits of the prefrontal cortex in schizophrenia. Among the several risk factors for the development of schizophrenia, stress during the prenatal period has been identified as crucial. Thus, it is proposed that prenatal stress induces neurodevelopmental alterations in the... |
| Tipo: Journal article |
Palavras-chave: Schizophrenia; Prefrontal cortex; Prenatal stress; Functional connectivity; DNA methylation; Reelin. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100016 |
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| Bezerra,Stephane Flaviane de Oliveira; Costa,Ludimila de Araújo; Freitas,Priscylla Alves Nascimento de; Oliveira,Naila Francis Paulo de. |
| The purpose of this study was to investigate the effect of aging on the DNA methylation status of two genes involved in tumorigenesis (telomerase gene hTERT and DNA repair gene- MLH1) and one in metabolism (methylenetetrahydrofolate reductase gene- MTHFR) in oral epithelial cells. DNA methylation analysis was performed by Methylation Sensitive Restriction Enzymes (MSRE) of healthy oral epithelial cells of child (6-10 years, n=21), young (20-25 years, n=19) and elderly (over 60 years, n=25). The results for the hTERT gene showed significant variation in the methylation frequency at CpG dinucleotides among the groups (p=0.0001), with the methylated condition more frequently in children and young people. In relation to MLH1 and MTHFR, no differences were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epigenetic; DNA methylation; Aging; DNA repair; Telomerase; MTHFR. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100082 |
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| Rastogi,Meenal; Gupta,Sameer; Sachan,Manisha. |
| ABSTRACT Ovarian carcinoma accounts for highest mortality of all gynecologic malignancies as the disease is asymptomatic until late stages. Biomarkers such as CA-125 and HE4 are being currently used for diagnosis of ovarian cancer, but they show contradicting diagnostic accuracy. Therefore other biomarkers have been investigated for early detection of this disease, but no success has been obtained and no test has yet been recommended for screening a general population. In this instance, aptamers can be effectively used to identify tumor-specific antigens for early diagnosis and targeted therapy of ovarian cancer. This article provides an overview of the biomarkers/panels being explored as well as the potential of aptamers to improve current long-term... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovarian cancer; Aptamers; DNA methylation; CA-125; HE4. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100346 |
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| Araújo,A.; Ramos,E.S.. |
| The high abortion rate of 45,X embryos indicates that patients with Turner syndrome and 45,X karyotype could be mosaics, in at least one phase of embryo development or cellular lineage, due to the need for the other sex chromosome presence for conceptus to be compatible with life. In cases of structural chromosomal aberrations or hidden mosaicism, conventional cytogenetic techniques can be ineffective and molecular investigation is indicated. Two hundred and fifty patients with Turner syndrome stigmata were studied and 36 who had female genitalia and had been cytogenetically diagnosed as having "pure" 45,X karyotype were selected after 100 metaphases were analyzed in order to exclude mosaicism and the presence of genomic Y-specific sequences (SRY, TSPY,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Turner syndrome; X-inactivation; HUMARA assay; DNA methylation; Criptic mosaicism. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500004 |
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| Lima,E.M.; Leal,M.F.; Burbano,R.R.; Khayat,A.S.; Assumpção,P.P.; Bello,M.J.; Rey,J.A.; Smith,M.A.C.; Casartelli,C.. |
| Gastric cancer is the forth most frequent malignancy and the second most common cause of cancer death worldwide. DNA methylation is the most studied epigenetic alteration, occurring through a methyl radical addition to the cytosine base adjacent to guanine. Many tumor genes are inactivated by DNA methylation in gastric cancer. We evaluated the DNA methylation status of ANAPC1, CDKN2A and TP53 by methylation-specific PCR in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosa in individuals from Northern Brazil. All gastric cancer samples were advanced stage adenocarcinomas. Gastric samples were surgically obtained at the João de Barros Barreto University Hospital, State of Pará, and were stored at -80°C before DNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA methylation; Gastric cancer; ANAPC1; CDKN2A; TP53. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600017 |
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| Wu,Wen-Chih; Hsu,Chih-Hsiung; Kuan,Jen-Chun; Hsieh,Jih-Fu; Sun,Chien-An; Yang,Tsan; Wu,Chang-Chieh; Chou,Yu-Ching. |
| A new noninvasive screening tool for colorectal neoplasia detects epigenetic alterations exhibited by gastrointestinal tumor cells shed into stool. There is insufficient existing data to determine temporal associations between colorectal cancer (CRC) progression and aberrant DNA methylation. To evaluate the feasibility of using fecal DNA methylation status to determine CRC progression, we collected stool samples from 14 male SD rats aged six weeks, and administered subcutaneous injections of either 1,2-dimethylhydrazine or saline weekly. p16 DNA methylation statuses in tumorous and normal colon tissue, and from stool samples were determined using methylation-specific PCR. Additionally, p16 methylation was detected in stool DNA from 85.7% of the CRC rats.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colorectal cancer; DNA methylation; Stool test for colorectal cancer. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300004 |
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| Wang,Dongxu; Wang,Guodong; Yang,Hao; Liu,Haibo; Li,Cuie; Li,Xiaolan; Lin,Chao; Song,Yuning; Li,Zhanjun; Liu,Dianfeng. |
| Abstract The sexually dimorphic expression of H19/IGF2 is evolutionarily conserved. To investigate whether the expression of H19/IGF2 in the female porcine eye is sex-dependent, gene expression and methylation status were evaluated using quantitative real-time PCR (qPCR) and bisulfite sequencing PCR (BSP). We hypothesized that H19/IGF2 might exhibit a different DNA methylation status in the female eye. In order to evaluate our hypothesis, parthenogenetic (PA) cells were used for analysis by qPCR and BSP. Our results showed that H19 and IGF2 were over-expressed in the female eye compared with the male eye (3-fold and 2-fold, respectively). We observed a normal monoallelic methylation pattern for H19 differentially methylated regions (DMRs). Compared with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: H19/IGF2; Gene expression; DNA methylation; Parthenogenetic; Pig. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100153 |
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| Guo,Xuhu; Xie,Qian; Li,Baoyuan; Su,Huanzhen. |
| Abstract DNA methylation plays an important role in plant growth and development, gene expression regulation, and maintenance of genome stability. However, only little information regarding stress-related DNA methyltransferases (MTases) genes is available in tomato. Here, we report the analysis of nine tomato MTases, which were categorized into four known subfamilies. Structural analysis suggested their DNA methylase domains are highly conserved, whereas the N-terminals are divergent. Tissue-specific analysis of these MTase genes revealed that SlCMT2, SlCMT3, and SlDRM5 were expressed higher in young leaves, while SlMET1, SlCMT4, SlDRM7, and SlDRM8 were highly expressed in immature green fruit, and their expression declined continuously with further fruit... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA methylation; Tissue-specific expression; Abiotic stress; Fruit ripening; Tomato. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100307 |
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| Du,Yue-feng; Liang,Liang; Shi,Ying; Long,Qing-zhi; Zeng,Jin; Wang,Xin-yang; He,Da-lin. |
| Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells. The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis. DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior. Hence, DNMT3B alone apparently plays a key role in maintaining the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Prostate cancer; DNA methylation; DNMT3; RNA interference. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100024 |
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| Cardoso-Júnior,Carlos A.M.; Fujimura,Patrícia Tieme; Santos-Júnior,Célio Dias; Borges,Naiara Araújo; Ueira-Vieira,Carlos; Hartfelder,Klaus; Goulart,Luiz Ricardo; Bonetti,Ana Maria. |
| Abstract Stingless bees of the genus Melipona, have long been considered an enigmatic case among social insects for their mode of caste determination, where in addition to larval food type and quantity, the genotype also has a saying, as proposed over 50 years ago by Warwick E. Kerr. Several attempts have since tried to test his Mendelian two-loci/two-alleles segregation hypothesis, but only recently a single gene crucial for sex determination in bees was evidenced to be sex-specifically spliced and also caste-specifically expressed in a Melipona species. Since alternative splicing is frequently associated with epigenetic marks, and the epigenetic status plays a major role in setting the caste phenotype in the honey bee, we investigated here epigenetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA methylation; Histone modification; Caste development; Social bees; Genetic caste determination. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100061 |
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| Anastasiadi, Dafni; Vandeputte, Marc; Sanchez-baizan, Nuria; Allal, Francois; Piferrer, Francesc. |
| The integration of genomic and environmental influences into methylation patterns to bring about a phenotype is of central interest in developmental epigenetics, but many details are still unclear. The sex ratios of the species used here, the European sea bass, are determined by genetic and temperature influences. We created four families from parents known to produce offspring with different sex ratios, exposed larvae to masculinizing temperatures and examined, in juvenile gonads, the DNA methylation of seven genes related to sexual development by a targeted sequencing approach. The genes most affected by both genetics and environment were cyp19a1a and dmrt1, with contrasting sex-specific methylation and temperature responses. The relationship between... |
| Tipo: Text |
Palavras-chave: Early development; Environmental temperature; DNA methylation; Sex determination; Aromatase; Cyp19a1a; Dmrt1; Epigenetic marks; DNA methylation threshold; Epigenetic inheritance. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00470/58123/61657.pdf |
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| Registros recuperados: 28 | |
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