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Investigação do potencial antioxidante e anticolinesterásico de 20 espécies da família Lauraceae 36
Yamaguchi,Klenicy Kazumy de Lima; Alcântara,Joelma Moreira; Veiga Junior,Valdir Florêncio da.
Lauraceae é uma das famílias mais importantes na floresta amazônica. Diversas de suas espécies apresentam em sua composição substâncias com pronunciadas atividades biológicas. Nesse trabalho, os extratos obtidos em etanol de galhos e folhas de 20 espécies de Lauraceae foram coletados na Reserva Florestal Ducke e avaliados quant o às atividades antioxidante frente ao radical livre 2,2-difenil-1-picrilhidrazila(DPPH) e inibidora da enzima acetilcolinesterase. O extrato dos galhos de Licaria martinianal apresentou a maior capacidade de sequestro do radical DPPH (6,96 µg.mL-1),mas os extratos de Ocotea iminor foram os mais ativos na inibição da enzima acetilcolinesterase, apresentando também pronunciada atividade antioxidante. O perfil de atividade...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lauraceae; DPPH; Anticolinesterásico; Licaria martinianal; Ocotea iminor.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672012000400012
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ACTIVIDAD ANTIOXIDANTE Y PERFIL DE ÁCIDOS GRASOS DE LAS SEMILLAS DE JABUTICABA (Myrciaria cauliflora BERG) 37
NEUZA,JORGE; BRUNA,JORGE BERTANHA; MORENO LUZIA,DÉBORA MARIA.
Múltiples compuestos naturales encontrados en frutas, cereales y vegetales presentan actividad antioxidante. Este trabajo tuvo como objetivo caracterizar las semillas de jabuticaba (Myrciaria cauliflora Berg) en cuanto a su composición proximal y potencial antioxidante, y evaluar el perfil de ácidos grasos en el aceite extraído de las mismas. Para la obtención del extracto, las semillas deshidratadas y trituradas fueron extraídas con alcohol etílico por 30 minutos, en la proporción de 1:3 de semillas:alcohol etílico, bajo agitación continua, a temperatura ambiente. Seguidamente, la mezcla fue filtrada y el sobrenadante deshidratado a 40 ºC con la finalidad de determinar, por pesaje directo, rendimiento en materia seca del extracto. De acuerdo con los...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Semillas; Aceites comestibles; Actividad antioxidante; Compuestos fenólicos; DPPH; Frutas.
Ano: 2011 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2011000200006
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Phenolic content and antioxidant activity of parts of Passiflora edulis as a function of plant developmental stage 39
Guimarães,Sarah F.; Lima,Inorbert M.; Modolo,Luzia V..
ABSTRACT Passiflora edulis Sims var. flavicarpa O. Deg. (Yellow-passion fruit) is the native plant species most used by juice industries in Brazil while its leaf extracts are widely employed in folk medicine. This study evaluated the phenolic content of leaves, roots, fruit shells and pulp of plants of P. edulis in juvenile, flowering and fruiting stages. The extent of scavenging and/or degradation of reactive nitrogen and oxygen species by plant extracts was also investigated. Leaves were the organs that most accumulated phenolics/flavonoids, regardless of plant developmental stage. Leaf extracts efficiently scavenged DPPH by up to 67 % while root and fruit shell extracts effectively captured up to 80 % O2 -. Maximum activity of catalase (51.6 mmol H2O2...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant enzymes; DPPH; Flavonoids; Passion fruit; Secondary metabolism; Superoxide anion.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000100074
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The influence of planting and harvesting times on the total phenolic content and antioxidant activity of Talinum triangulare (Jacq.) Willd 1
Brasileiro, Beatriz Gonçalves; Leite, João Paulo Viana; Casali, Vicente Wagner Dias; Pizziolo, Virgínia Ramos; Coelho, Olívia Gonçalves Leão.
The purpose of this study was to evaluate the influence of planting and harvesting times on the polyphenol content and antioxidant capacity of Talinum triangulare cultivated during two different seasons (winter or summer) and harvested 30 or 60 days after seedling establishment. Polyphenol content was quantified with the Folin-Ciocalteu method, and antioxidant activity was quantified with free-radical DPPH (1,1-diphenyl-2-picrilhidrazina). The highest levels of polyphenols were obtained from winter planting and from harvesting at 30 days. Antioxidant activity differed significantly in response to variation in planting and harvesting times, reaching 56.97% in extracts of plants produced in the winter and harvested at 30 days after planting. The times of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.03.00-8 portulacaceae; Waterleaf; DPPH; Polyphenol Fitotecnia e Plantas medicinais.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19130
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Volatile chemical composition and bioactivities from Colombian Kyllinga pumila Michx (Cyperaceae) essential oil 3
Jaramillo-Colorado, Beatriz Eugenia; Martínez-Cáceres, Eduardo Luis; Duarte-Restrepo, Edisson.
The essential oil from the fresh leaves of Kyllinga pumila (Michx) was obtained by hydrodistillation and characterized by gas chromatography-mass spectrometry (GC-MS). Twenty-eight volatile compounds were identified, major constituents of the oil were Methyl E,E-10,11-epoxyfarnesoate (43.8%), β-elemene (12.5%), Z-caryophyllene (11.3%), germacrene D (7.1%) and E-caryophyllene (5.6%). Repellent and fumigant activities of the oil against Tribolium castaneum Herbst (Coleoptera: Tenebrionidae), were done using the area preference method. Additionally, we studied their antioxidant and phytotoxic effects. Essential oils exhibited a dose-dependent repellent activity, with values 90% at the applied concentration (0.01%), for both two and four hour’s exposure....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Essential oils; Repellent activity; Fumigant; Tribolium castaneum; Phytotoxic; DPPH.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/32386
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Antioxidant activity and flavonoid content of Clusia fluminensis Planch. & Triana 42
Silva,Maria Carolina A. da; Paiva,Selma R..
Clusia fluminensis Planch. & Triana (Clusiaceae Lindl.) is a native species found in regions of high luminosity and water restriction. The aim of this study was to evaluate the antioxidant activity of Clusia fluminensis crude extracts through the scavenging of the stable free radical DPPH, and the determination of flavones and flavonols content in these extracts. The fruit acetonic extract showed the lowest EC50 value (2.71 ± 0.34 g extract / g DPPH), the lowest percentage of remaining DPPH at the concentrations of 125 and 250 µg/mL (about 4% in both), and also the greatest percentage of flavones and flavonols (13.93 ± 0.21 %). Statistical analysis suggests a positive correlation between the presence of flavonoids and the antioxidant activity of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aluminum chloride; Clusia fluminensis; DPPH; Flavonoids.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300004
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Estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia 48
Paudel,Babita; Bhattarai,Hari Datta; Chan Kim,Il; Lee,Hyoungseok; Sofronov,Roman; Ivanova,Lena; Poryadina,Lena; Yim,Joung Han.
BACKGROUND: Several plants are reported to be produced various biological active compounds. Lichens from the extreme environments such as high altitude, high UV, drought and cold are believed to be synthesized unique types of secondary metabolites than the other one. Several human pathogenic bacteria and fungi have been muted into drug resistant strains. Various synthetic antioxidant compounds have posed carcinogenic effects. This phenomenon needs further research for new effective drugs of natural origin. This manuscript aimed to screen new source of biological active compounds from plants of subarctic origin. RESULTS: A total of 114 plant species, including 80 species of higher plants, 19 species of lichens and 15 species of mosses, were collected from...
Tipo: Journal article Palavras-chave: Antimicrobial; Antioxidant; Brine shrimp; DPPH; Lichen; Moss.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100010
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Antioxidant and anticholinesterase investigations of Rumex hastatus D. Don: potential effectiveness in oxidative stress and neurological disorders 48
Ahmad,Sajjad; Ullah,Farhat; Ayaz,Muhammad; Sadiq,Abdul; Imran,Muhammad.
BACKGROUND: Rumex species are traditionally used for the treatment of neurological disorders including headache, migraine, depression, paralysis etc. Several species have been scientifically validated for antioxidant and anticholinestrase potentials. This study aims to investigate Rumex hastatus D. Don crude methanolic extract, subsequent fractions, saponins and flavonoids for acetylcholinestrase, butyrylcholinestrase inhibition and diverse antioxidant activities to validate its folkloric uses in neurological disorders. Rumexhastatus crude methanolic extract (Rh. Cr), subsequent fractions; n-hexane (Rh. Hex), chloroform (Rh. Chf), ethyl acetate (Rh. EtAc), aqueous fraction (Rh. Aq), crude saponins (Rh. Sp) and flavonoids (Rh. Fl) were investigated against...
Tipo: Journal article Palavras-chave: Ethnomedicinal; Acetylcholinesterase; Butyrycholinesterase; DPPH; ABTS.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100020
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Free radical scavenging activity of ethanolic extracts from herbs and spices commercialized in Brazil 52
Mariutti,Lilian Regina Barros; Barreto,Gisela Pizarro de Mattos; Bragagnolo,Neura; Mercadante,Adriana Zerlotti.
Ethanolic extracts from 23 different dried herbs and spices commercialized in Brazil were investigated for their free radical scavenging properties using the stable free radicals 2,2'-diphenyl-β-picrylhydrazyl (DPPH•) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS•+), and Trolox as reference (TEAC) for both radicals. The kinetic curves for both radicals showed to follow the first order kinetics model and the decay rate constant (k obs) was calculated. For all the samples, the two methods showed a good linear TEAC correlation, indicating that the average reactivity of the compounds present in the ethanolic extracts was similar. Sage and rosemary extracts showed the highest free radical scavenging capacities, while onion showed negligible...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant activity; Free radical scavenging activity; DPPH; ABTS; Herbs; Spices; Colorifico.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600018
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Antioxidant properties of some filamentous green algae (Chaetomorpha Genus) 52
Farasat,Massoumeh; Khavari-Nejad,Ramazan-Ali; Nabavi,Seyed Mohammad Bagher; Namjooyan,Foroogh.
The antioxidant activity and the contents of total phenolics and flavonoids were quantified in the methanolic extracts of four Chaetomorpha species including C. aerea, C. crassa, C. linum and C. brachygona. Eight samples of Chaetomorpha plants were collected from five locations along the northern coasts of the Persian Gulf in south of Iran from December 2010 until October 2011. Methanolic extracts of the seaweeds were assessed for their antioxidant activity using DPPH radical scavenging assay. C. linum showed highest antioxidant potential with a relatively low IC50 (1.484 ± 0.168 mg mL-1), the highest flavonoid content (18.177 ± 2.238 mg RE g-1 ) and a relatively high content of phenolics (2.895 ± 0.415 mg GAE g-1) in comparison with the other species. C....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant; Phenolics; Flavonoid; DPPH; Green algae; Chaetomorpha.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600005
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Stability of phenolic compounds and antioxidant capacity of regular and decaffeinated coffees 52
Vicente,Silvio José Valadão; Queiroz,Yara Severino; Gotlieb,Sabina Léa Davidson; Torres,Elizabeth Aparecida Ferraz da Silva.
This study compared the regular and decaffeinated coffees in relation to antioxidant capacity, levels of some antioxidant molecules and stability of these parameters over a six-month period under different storage conditions. The regular coffee samples analyzed right after the industrial production showed higher antioxidant capacity (ORAC and DPPH), the same levels of phenolic compounds and higher levels of phenolic acids than decaffeinated coffee. After six months, the closed packs of both the grades kept under vacuum at 20°C did not show significant changes from the initial results; the open packs stored at 4°C showed small but statistically significant reductions and the open packs stored at 20°C showed higher and statistically significant losses (p...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffee; Antioxidant capacity; Phenolic compounds; Caffeine; ORAC; DPPH.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100016
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Antioxidant Activities of Chicken Egg White Hydrolysates Obtained by New Purified Protease of Aspergillus avenaceus URM 6706 52
Silva,Anna Carolina da; Queiroz,Alana Emilia Soares de França; Oliveira,João Tiago Correia; Medeiros,Erika Valente; Souza-Motta,Cristina Maria de; Moreira,Keila Aparecida.
Abstract Protein hydrolysates originating from egg white have already been reported to be bioactive and, among their biological activities, possess the antioxidant property that protects the body from early ageing and diseases linked to oxidation. Therefore, the objective of this work was to evaluate the antioxidant activity of hydrolysates obtained by the hydrolysis of egg white from hen poultry. The protease produced by Aspergillus avenaceus URM 6706 was purified and subsequently applied to hydrolysate the egg white, and the degree of hydrolysis was verified during the protease exposure time (4-24 h). The hydrolysis was intensified over time of exposure to the protease. It was possible to detect the antioxidant activities of eliminating the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus; Purification; ABTS; +; DPPH; Chelators.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100508
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Antioxidant activity of Agaricus brasiliensis basidiocarps on different maturation phases 58
Mourão,Francielly; Umeo,Suzana Harue; Takemura,Orlando Seiko; Linde,Giani Andrea; Colauto,Nelson Barros.
Different maturation phases of basidiocarp could affect the bioactivity and concentration of some active substances. A. brasiliensis Wasser et al.(A. blazei Murrill) has shown antitumor activity that could be related to the antioxidant activity. However there is no information of the best basidiocarp maturation phase for extracting antioxidant substances in order to determine the moment of harvesting in mushroom cultivation. The objective of this work was to evaluate the antioxidant activity of A. brasiliensis strains on different basidiocarp maturation phases. The best condition for extraction of A. brasiliensis antioxidants is with methanol as solvent at 60 ºC for 60 min. Strains with closed basidiocarp have higher antioxidant activity than with opened...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Absorbance; Antioxidant activity; Basidiomycete; Agaricus blazei; DPPH.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100024
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Bioisosteric modification on melatonin: synthesis of new naphthalene derivatives, in vitro antioxidant activity and cytotoxicity studies 60
Shirinzadeh,Hanif; Ghalia,Mohammed; Tascioglu,Alev; Adjali,Ferial Intissar; Gunesacar,Gulsen; Gurer-Orhan,Hande; Suzen,Sibel.
Melatonin (MLT) is a strong free radical scavenger that protects the body from the deleterious effects of excess oxidants. Synthesis of MLT analogue compounds with antioxidant potency has recently attracted the interest of researchers. In general, the strategy consists of modifying the groups in the different sites of the indole ring or replacing the indole ring with an analogue. As part of our ongoing research, the antioxidant capacity and cytotoxicity of newly synthesized MLT analogue naphthalene derivatives were evaluated. The radical scavenging activity was tested by a 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Most of the synthesized compounds showed significant antioxidant activity in comparison to MLT. The structure-activity relationship was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant; Cytotoxic; Melatonin; MTT; DPPH.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100550
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Maceration extraction conditions for determining the phenolic compounds and the antioxidant activity of Catharanthus roseus (L.) G. Don 64
Cacique,Ane Patrícia; Barbosa,Érica Soares; Pinho,Gevany Paulino de; Silvério,Flaviano Oliveira.
ABSTRACT Catharanthus roseus(L.) G. Don is a medicinal plant known for its important constituent aromatic compounds. In the literature, no detailed studies elucidating the methods of extraction of phenolic compounds for this species are available. Therefore, the present study was aimed at evaluating the maceration parameters for determining the total phenols content in C. roseus. The parameters of plant mass, extraction phase composition, homogenization type, extraction time, and temperature were evaluated in terms of improving the extraction conditions. The plant extracts were subjected to specific spectrophotometric analysis designed for each parameter, and the total phenols content was determined. After establishing the optimal conditions, the extracts...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phytochemistry; DPPH; ABTS; FRAP; Phenolic acids..
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542020000100245
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Quantificação de ácido alfa-linolênico em caules e folhas de linho (Linum usitatissimum L.) colhidos em diferentes estágios de desenvolvimento 64
Aguiar,Ana Carolina de; Aguiar,Sílvia Cristina de; Boroski,Marcela; Souza,Nilson Evelázio de; Matsushita,Makoto; Visentainer,Jesuí Vergílio.
O objetivo deste estudo foi quantificar o ácido alfa-linolênico [LNA, 18:3 (n-3)], avaliar a composição centesimal das folhas e caules de linho (Linum usitatissimum L.) submetidos à secagem e colhidos em diferentes estágios de desenvolvimento (40, 80 e 120 dias), e determinar o potencial antioxidante das folhas colhidas aos 80 dias através do teste com o radical DPPH. As folhas obtiveram maiores teores de cinzas, proteína e lipídios totais em relação aos caules. Tanto as folhas quanto os caules apresentaram razões de AGPI/AGS e n-6/n-3 dentro dos valores considerados adequados para a alimentação. Os caules colhidos nos diferentes tempos não apresentaram diferenças significativas (P<0,05), quanto ao teor de LNA. As folhas colhidas aos 80 dias...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ômega-3; Ácidos graxos; Atividade antioxidante; DPPH.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000600021
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The effect of foliar boron spraying on the fruit features of Brigitta and Legacy highbush blueberry (Vaccinium corymbosum) cultivars 118
Meriño-Gergichevich,Cristian; Pacheco,Eduardo; Reyes-Díaz,Marjorie.
C. Meriño-Gergichevich, E. Pacheco, and M. Reyes-Díaz. 2016. The effect of foliar boron spraying on the fruit features of Brigitta and Legacy highbush blueberry (Vaccinium corymbosum) cultivars. Cien. Inv. Agr. 43(3):452-463. Boron (B) is associated with reproductive and antioxidant metabolism in plants, and in southern Chile, the deficiency of this micronutrient is ameliorated through foliar applications that are a part of agricultural management, thus ensuring the productivity of crops including highbush blueberry (Vaccinium corymbosum L.). However, the appropriate B dosage for this species is unknown, so the aim of this study was to evaluate foliar B application on fruit quality in highbush blueberry in southern Chile. During the 2014-2015 season,...
Tipo: Journal article Palavras-chave: Antioxidant; DPPH; Fruit quality; Micronutrient; ORAC.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000300011
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Comparison of the total phenolic content, total anthocyanin content and antioxidant activity of polyphenol-rich fruits grown in Chile 118
Fredes,Carolina; Montenegro,Gloria; Zoffoli,Juan Pablo; Santander,Francisca; Robert,Paz.
In the last 10 years, interest in research on polyphenol-rich fruit species has increased due to the potential health benefits of these species, mainly attributed to their high anthocyanin content and antioxidant activity. Six polyphenol-rich fruit species (blueberries, raspberries, blackberries, strawberries, pomegranates and maqui berries) were harvested at the same maturity stage during the same growing season and were compared according to their total phenolic (Folin-Ciocalteu method) and total anthocyanin (pH differential method) contents and antioxidant activity using ferric-reducing antioxidant power (FRAP assay) and 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging capacity methods. With the results of this study, the polyphenolic status of the...
Tipo: Journal article Palavras-chave: Berries; DPPH; FRAP; Maqui; Pomegranate.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202014000100005
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Atividade antioxidante e teor de fenólicos totais em hortaliças orgânicas e convencionais 116
Arbos,Kettelin Aparecida; Freitas,Renato João Sossela de; Stertz,Sônia Cachoeira; Dornas,Marlos Ferreira.
Considerando que os consumidores estão mais conscientes com a sua saúde e mais preocupados com as condições ambientais, verifica-se uma crescente demanda por alimentos orgânicos. O objetivo deste estudo foi comparar a atividade antioxidante e o teor de fenólicos totais entre alface, rúcula e almeirão de cultivos orgânico e convencional, plantados em uma mesma horta. A atividade antioxidante dos extratos metanólicos das hortaliças foi determinada através do ensaio do DPPH. O teor de fenólicos totais foi obtido espectrofotometricamente de acordo com o método de Folin-Ciocalteu e calculado como equivalente de ácido gálico. A atividade antioxidante contra o radical livre DPPH foi: rúcula orgânica > almeirão orgânico > alface orgânica > rúcula...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hortaliças; Ação antioxidante; Sistema de produção; DPPH; Fenólicos.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000200031
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A novel procedure to measure the antioxidant capacity of Yerba maté extracts 116
Hartwig,Vanessa Graciela; Brumovsky,Luis Alberto; Fretes,Raquel María; Boado,Lucila Sánchez.
Yerba maté extracts have in vitro antioxidant capacity attributed to the presence of polyphenolic compounds, mainly chlorogenic acids and dicaffeoylquinic acid derivatives. DPPH is one of the most used assays to measure the antioxidant capacity of pure compounds and plant extracts. It is difficult to compare the results between studies because this assay is applied in too many different conditions by the different research groups. Thus, in order to assess the antioxidant capacity of yerba maté extracts, the following procedure is proposed: 100 µL of an aqueous dilution of the extracts is mixed in duplicate with 3.0 mL of a DPPH 'work solution in absolute methanol (100 µM.L-1), with an incubation time of 120 minutes in darkness at 37 ± 1 °C, and then...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DPPH; Yerba maté; Antioxidant capacity; Ilex paraguariensis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100019
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