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| Registros recuperados: 89 | |
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| Tu,Zhiming; He,Guangyuan; Li,Kexiu X.; Chen,Mingjie J.; Chang,Junli; Chen,Ling; Yao,Qing; Liu,Dongping P.; Ye,Huan; Shi,Jiantao; Wu,Xuqian . |
| This paper describes an efficient bacterial transformation system that was established for the preparation of competent cells, plasmid preparation, and for the storage in bacterial stocks in our laboratory. Using this method, a number of different plasmids have been amplified for further experiments. Competent cells for bacterial transformation were prepared by the calcium chloride method with an optimum concentration of 75 mM. Three different strains of Escherichia coli that were tested are DH5α, TG1 and XL1 blue, and the most efficient strain being XL1 blue. The optimal optical density (OD600) range for competent cell preparation varied for each of the strains investigated, and for XL1 blue it was 0.15-0.45; for TG1 it was 0.2-0.5; and for... |
| Tipo: Journal article |
Palavras-chave: Competent cells; E. coli; Plasmid; Storage; Transformation. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100014 |
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| Casablancas,Antoni; Cárdenas-Fernández,Max; Álvaro,Gregorio; Benaiges,Maria Dolors; Caminal,Glòria; Mas,Carles de; González,Glòria; López,Carmen; López-Santín,Josep. |
| Background: New enzymes for biotransformations can be obtained by different approaches including directed mutagenesis and in vitro evolution. These mutants have to be efficiently produced for laboratory research on bioreactions as well as for process development. In the framework of a European ERA-IB project, two different types of enzymes (ammonia lyases and aminotransferases) have been selected as biocatalysts for the synthesis of industrially relevant amines. New mutant enzymes have been obtained: a) aspartases able to recognize β-amino acids; b) ω-transaminases with improved activity. The objectives are to find out a common operational strategy applicable to different mutants expressed in E. coli with the same initial genetic... |
| Tipo: Journal article |
Palavras-chave: Amine transaminases; Ammonia lyases; E. coli; Fed-batch; Immobilized biocatalysts. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300004 |
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| Stoppe,Nancy de Castro; Silva,Juliana Saragiotto; Torres,Tatiana Teixeira; Carlos,Camila; Hachich,Elayse Maria; Sato,Maria Inês Zanoli; Saraiva,Antonio Mauro; Ottoboni,Laura Maria Mariscal. |
| Different types of water bodies, including lakes, streams, and coastal marine waters, are often susceptible to fecal contamination from a range of point and nonpoint sources, and have been evaluated using fecal indicator microorganisms. The most commonly used fecal indicator is Escherichia coli, but traditional cultivation methods do not allow discrimination of the source of pollution. The use of triplex PCR offers an approach that is fast and inexpensive, and here enabled the identification of phylogroups. The phylogenetic distribution of E. coli subgroups isolated from water samples revealed higher frequencies of subgroups A1 and B2(3) in rivers impacted by human pollution sources, while subgroups D1 and D2 were associated with pristine sites, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: E. coli; Phylogenetic groups; Pollution sources; Interaction networks; Social network analysis. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500012 |
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| Gervais, Hugo; Gautier, Emeric; Grizon, James; Morin, Dimitri; Soletchnik, Patrick; Piquet, Jean-come; Bruneau, Audrey. |
| Après un rappel des objectifs, du fonctionnement et de la méthode d'interprétation des résultats du réseau de contrôle microbiologique REMI et du réseau de surveillance chimique ROCCH, ce rapport décrit le programme annuel du département de la Vendée. Il présente l'ensemble des résultats obtenus sur la période 2017-2019, en particulier l'estimation de la qualité microbiologique et chimique des zones de production de coquillages classées. |
| Tipo: Text |
Palavras-chave: REMI; E. coli; Contamination bactériologique des coquillages; ROCCH; Contaminants chimiques; Milieu marin; Classement sanitaire des zones de production; Département de la Vendée (85); LERPC. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00632/74397/74099.pdf |
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| Bougeard, Morgane; Le Saux, Jean-claude; Perenne, Nicolas; Le Guyader, Soizick; Pommepuy, Monique. |
| During rainstorms, watersheds can introduce large amounts of faecal pollution into the rivers and sea, leading to shellfish contamination. In this study, we assessed Escherichia coli fluxes from a catchment, and their impact on estuarine water quality, using two assembled models. For the catchment, the agro-hydrological model SWAT was implemented integrating land uses, soil, topography, rainfall and other climatic data on Daoulas watershed (France). Initially, the SWAT model was calibrated and validated for river flow rates, and results were found satisfactory. Subsequently, different faecal contamination sources were integrated into the model: point sources (WWTP discharges into rivers) and non-point sources (manure spreading on fields). During rainfall... |
| Tipo: Text |
Palavras-chave: Modeling; SWAT; MARS; E. coli; Shellfish; Catchment; Estuary. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00066/17748/15270.pdf |
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| Ervais, Hugo; Gautier, Emeric; Grizon, James; Morin, Dimitri; Soletchnik, Patrick; Piquet, Jean-come; Bruneau, Audrey. |
| Après un rappel des objectifs, du fonctionnement et de la méthode d'interprétation des résultats du reseau de contôle microbiologique REMI et du réseau de surveillance chimique ROCCH, ce rapport décrit le programme annuel du département de la Charente-Maritime. Il présente l'ensemble des résultats obtenus sur la période 2017-2019, en particulier l'estimation de la qualité microbiologique et chimique des zones de production de coquillages classées |
| Tipo: Text |
Palavras-chave: REMI; E. coli; Contamination bactériologique des coquillages; ROCCH; Contaminants chimiques; Milieu marin; Classement sanitaire des zones de production; Département de la Charente Maritime (17); LERPC. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00631/74329/73967.pdf |
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| Crottier, Anais; Abadie, Eric; Brun, Melanie; Piquet, Jean-come; Fiandrino, Annie. |
| Après un rappel des objectifs, du fonctionnement et de la méthode d’interprétation des résultats des réseaux de contrôle microbiologique (REMI) et de surveillance chimique (ROCCH), ce rapport inclut un bilan national et décrit le programme annuel des départements de l’Hérault, du Gard et de l’Aude. Il présente l’ensemble des résultats obtenus, en particulier l’estimation de la qualité microbiologique et chimique des zones de production de coquillages classées pour la période 2015-2017. Sur les 29 zones de production conchylicole classées et suivies en Occitanie, la qualité a pu être estimée pour 23 d’entre elles. Selon les critères réglementaires pris en compte (règlement CE n°854/2004, modifié par le règlement n°2285/2015), dans les départements de... |
| Tipo: Text |
Palavras-chave: REMI; E. coli; Contamination bactériologique des coquillages; ROCCH; Contaminants chimiques; Milieu marin; Classement sanitaire des zones de production; Département de l’Hérault; Départemen tdu Gard; Département de l’Aude. |
| Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00445/55635/57280.pdf |
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| Registros recuperados: 89 | |
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