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Evidence of mutagenic and lethal effects of herbicides on Amazonian frogs 36
FERRANTE,Lucas; FEARNSIDE,Philip M..
ABSTRACT Amphibians are excellent bioindicators because they are sensitive to chemical pollution and can indicate ecosystem changes due to the presence of or exposure to chemical compounds. Here we report evidence of the impact of herbicides, including glyphosate, on amphibians in a locality in the central Brazilian Amazon and compare it with data from other biomes in Brazil. We observed malformations in three species of Leptodactylus and local extinctions of Scinax ruber and Rhinella marina from reproductive sites close to an area where herbicides had been applied. The observations in the Amazon are similar to reports from Brazil’s Atlantic forest on morphological anomalies and mortality in amphibians exposed to herbicides. We warn of the threat of...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Agrochemicals; Amazon Forest; Anurans; Bioindicators; Ecotoxicology; Environmental impact.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400363
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Comparison of nuclear abnormalities in Astyanax bifasciatus Cuvier, 1819 (Teleostei: Characidae) of two sections of rivers from the middle Iguaçu 3
Melo-Silva, Merieli; Oliveira, Felipe Rafael; Rosa, Jonathan; Gemelli, Emanuelli; Santos, Lilian; Bueno-Krawczyk, Ana Carolina de Deus.
Most changes in water bodies are result of human activities that have the potential to undermine the environmental integrity of aquatic ecosystems. Changes in genetic material can be evidenced by the frequency of nuclear abnormalities in fish blood cells, in response to genotoxic agents even at low concentrations. Thus, we aimed at comparing the frequencies of nuclear abnormalities of fish kept in acclimation, with fish collected in Timbó river (Santa Cruz do Timbó, Santa Catarina State), under low anthropogenic interference, and fish collected in Iguaçu river (União da Vitória, Paraná State), a polluted river. The highest frequency of changes in nuclear morphology of fish erythrocytes was found in the urbanized area around the Iguaçu river, while, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Micronucleus; Ecotoxicology; Mutagenesis; Pollution. Mutagênese.
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/40669
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Artificial tritrophic exposure system for environmental risk analysis on aphidophagous predators 42
PAULA,DÉBORA P.; SOUZA,LUCAS M. DE; ANDOW,DAVID A.; SOUSA,ALEX A.T. CORTÊS DE; PIRES,CARMEN S.S.; SUJII,EDISON R..
ABSTRACT We evaluated an artificial tritrophic exposure system for use in ecotoxicological evaluations of environmental stressors on aphidophagous predators. It consists of an acrylic tube with a Parafilm M sachet containing liquid aphid diet, into which can be added environmental stressors. Immature Cycloneda sanguinea, Harmonia axyridis and Chrysoperla externa, and adult H. axyridis were reared on Myzus persicae. Larval and pupal development and survival of all species and reproductive parameters of H. axyridis were similar to published results. The system provides a suitable tritrophic exposure route, enables ex-ante evaluation of stressors, and improves the accuracy of the assessment.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beneficial insects; Biosafety; Ecotoxicology; Exposure routes.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401569
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Clinical, biochemical and haemathological effects in Rhamdia quelen exposed to cypermethrin 45
Montanha,F.P.; Fredianelli,A.C.; Wagner,R.; Sacco,S.R.; Rocha,D.C.C.; Pimpão,C.T..
The acute intoxication of Cypermethrin in Silver Catfish (Rhamdia quelen) was evaluated. Animals weighing 56.67±4.43g and measuring 18.92±1.16cm were exposed to sublethal concentrations of Cypermethrin for the species in hydrological conditions during 96 hours. A total of 52 fish divided into three groups were used and received the following concentrations of Cypermethrin: 0 (n=12); 1.5 (n=20) and 2.5 (n=20)mg/L. The intoxicated animals suffered behavioral changes such as loss of balance, swimming alteration, dyspnea, upright swimming and sudden spiral swimming movements. As soon as the 96-hour period was over, a blood collection for hematological and biochemical analyses was performed. A complete haemogram test, plasmatic protein test, albumin, alanine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Biomarkers; Ecotoxicology; Haemogram; Pyrethroids.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300697
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Ecotoxicological studies on the pejerrey (Odontesthes bonariensis, Pisces Atherinopsidae) 47
Carriquiriborde,Pedro; Ronco,Alicia.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Odontesthes bonariensis; Ecotoxicology; Environmental pollution; Heavy metals; Pesticides.
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100012
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Chronic toxicity bioassay with populations of the crustacean Artemia salina exposed to the organophosphate diazinon 48
Bustos-Obregon,Eduardo; Vargas,Álvaro.
A chronic toxicity bioassay was conducted with the microcrustacean Artemia salina as the testing organism for the toxic organophosphate diazinon in order to determine if the species is an appropriate indicator of pollution in aquatic environments. Tests of animal exposure to different concentrations of the toxicant were performed for 24, 48 and 72 hours after larvae hatching. Registered mortality data was used to obtain the lethal dose 50 (LD50) of the organophosphate for each exposure time, considering the immobilization of A. salina larvae as the mortality parameter. The lethal concentration (LD50) in the same exposure times was calculated by evaluating morphological changes on the three initial stages of larval development. Both doses were determined by...
Tipo: Journal article Palavras-chave: Diazinon; Artemia salina; Ecotoxicology; Bioassay.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300013
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De novo transcriptome assembly, functional annotation and differential gene expression analysis of juvenile and adult E. fetida, a model oligochaete used in ecotoxicological studies 48
Thunders,Michelle; Cavanagh,Jo; Li,Yinsheng.
Abstract Background Earthworms are sensitive to toxic chemicals present in the soil and so are useful indicator organisms for soil health. Eisenia fetida are commonly used in ecotoxicological studies; therefore the assembly of a baseline transcriptome is important for subsequent analyses exploring the impact of toxin exposure on genome wide gene expression. Results This paper reports on the de novo transcriptome assembly of E. fetida using Trinity, a freely available software tool. Trinotate was used to carry out functional annotation of the Trinity generated transcriptome file and the transdecoder generated peptide sequence file along with BLASTX, BLASTP and HMMER searches and were loaded into a Sqlite3 database. To identify differentially expressed...
Tipo: Journal article Palavras-chave: Earthworm; Trinity; Transcriptome; E. fetida; Ecotoxicology; RNA; Sequence; Gene expression.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100401
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Sensitivity of the amphipod Tiburonella viscana (Platyischnopidae) to K2CR2O7 52
Abessa,Denis M. S.; Sousa,Eduinetty C. P. M..
The sensitivity of Tiburonella viscana to K2Cr2O7 was evaluated from 18 water-only toxicity tests. The mean 48h-LC50 was estimated as 11.217 ± 3.768 mg/l, and the warning limits were calculated (mean LC50 ± 2 SD) as being between 3.68 and 18.75 mg/l. These results are baseline for the species and can be used for future comparisons in toxicity tests.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tiburonella viscana; Potassium dichromate; Brazil; Ecotoxicology.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100009
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Comparison between four and seven-day Ceriodaphnia dubia survival and reproduction test protocols using oil refinery effluent samples 52
Oliveira-Filho,Eduardo Cyrino; Da-Matta,Anndressa Camillo; Cabral,Lucianna Lopes; Veiga,Letícia Falcão; Paumgartten,Francisco José Roma.
The aim of this study was to compare the 7-day Ceriodaphnia dubia survival and reproduction test with a 4 day test to evaluate the toxicity of oil refinery effluents. Both shown identical NOECs with three of 6 tested samples. With one of the tested samples, the 4-day NOEC was one tested-concentration lower, and with the remaining two samples, it was one tested-concentration higher than those obtained using the 7-day protocol. Then results suggested that 4 and 7-day protocols were comparable to detect toxic effects.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aquatic toxicology; Water pollution; Ecotoxicology; Oil refinery effluents; Reproduction; Aquatic chronic tests.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100017
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Study of the effect of Aliette on the activity of spinach (Spinacea oleracea L.) chloroplasts 61
Almeida,Pedro; Coutinho,João P.; Rocha,João; Gomes-Laranjo,José.
Intensification of production in modern agriculture is frequently obtained thanks to the increase of pesticide applications and chemical fertilizers. However, the use of these products sometimes causes a reduction of crop productivity. We aimed to evaluate the impact of the fungicide Aliette on in vitro activity of chloroplasts of spinach (Spinacea oleracea L.). Transmembrane potential formation, PSII oxygen evolution and PSII photochemistry were analyzed. Our results showed that the recommended concentration (2.5 g L-1) of Aliette did not impair the photosynthetic apparatus. However, at higher concentrations (> 7.5 g L-1), reductions in thylakoid membrane potential and oxygen evolution were recorded, but with no evidence of photoinhibition.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioenergetics; Ecotoxicology; Pesticides; Photosynthesis; Xenobiotics.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000100009
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Toxicological effect from the stem cortex of the amazonic plant soapberry Paullinia clavigera (Sapindaceae) upon three arthropods 118
Pérez,Diana; lannacone,José; Pinedo,Harvey.
Toxicological efifects of four extracts proceeding from the stem cortex of the amazonic plant Paullinia clavigera D.R. Simpson (Sapindaceae) by decoction, ethanolic, chlorophormic and hexanic on three arthropods: Rhynchophoruspalmarían (Linnaeus, 1758) (Curculionidae), Eupalamides cyparissias (Fabricius, 1777) (Castniidae) and Artemia franciscana (Kellog, 1906) (Artemiidae) in Ucayali, Peru were studied. The four extracts at the highest concentration tested were: decoction at a proportion 1:10 (w/v), ethanolic, chlorophormic and hexanic at 100 mg·L-1. Toxic effects were evaluated at 12,24,48 and 72 hours on larvae of III in star of R. palmarum and larvae of II instar of E. cyparissias; and at 24 and 48 hours on nauplii of A. franciscana. Toxicity in terms...
Tipo: Journal article Palavras-chave: Artemia franciscana; Botanical insecticide; Ecotoxicology; Eupalamides cyparissias; Paullinia clavigera; Rhynchophorus palmarum.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000300012
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Evaluation of eugenol toxicity in bioassays with test-organisms 65
Gueretz,Juliano Santos; Somensi,Cleder Alexandre; Martins,Maurício Laterça; Souza,Antonio Pereira de.
ABSTRACT: Fish in both natural and farming conditions are exposed to stress of capture, handling, transport or treatment that provoke low zootechnical performance. Anesthetics like eugenol obtained from clove oil have been used strategically not only in freshwater but also in marine and estuarine fish in order to reduce the stress. Apart from the eugenol indication as anesthetic and its low toxicity for animals, its environment action is not clear. Bioassays or ecotoxicity tests with indicator organisms are used to evaluate the mode of action of the pollutants in the environment. The aim of this study was to test the acute toxicity of eugenol using the microcrustacean Daphnia magna and the bacterium Aliivibrio fischeri, and also its chronic toxicity for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aliivibrio fischeri; Daphnia magna; Desmodesmus subspicatus; Ecotoxicology.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200403
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Cadmium and Lead content in Liver and Kidney tissues of Wild Turkey Vulture Cathartes aura (Linneo, 1758) from Chañaral, Atacama desert, Chile 72
Valladares F,Pablo; Alvarado,Sergio; Urra R,Carolina; Abarca,Jorge; Inostroza,Jorge; Codoceo,Juana; Ruz,Manuel.
The Atacama region, Chile, presents one of the highest levels of mining exploitation of the country, which leads to high levels of contamination from mine tailings and other related environmental liabilities. One of the most complex situations occurred in the Chañaral city, north of Chile, where for over 50 years mine tailings were dumped on the coast, causing severe damage in the ecosystem. To evaluate the effects on terrestrial biota, we analyzed the concentration of cadmium and lead in tissues of Turkey Vulture (Cathartes aura). The results indicate that accumulation of cadmium in kidney was 10.31 μg/g (SD 8.00, range 0.27 to 20.73 μg/g) while in the liver was 5.24 μg/g (SD 8.00, range 0.49 to 19.70). There values are very...
Tipo: Journal article Palavras-chave: Ecotoxicology; Heavy Metals; Cathartes aura.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382013000200004
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Avaliação toxicopatológica de biopesticidas em roedores: revisão dos ensaios da fase 1. 14
CASTRO, V. L. S. S. de.
O propósito do presente trabalho é apresentar alguns cuidados a serem tomados durante a condução de testes toxicológicos para biopesticidas cujos organismos sejam bactérias ou fungos, como também apresentar sugestões quanto ao aprimoramento metodológico dos mesmos. Nesta publicação, portanto, são apresentados aspectos relativos ao controle de qualidade laboratorial e à avaliação do método usado para plaqueamento e crescimento dos AMC ? fungos e bactérias, com base na experiência do Laboratório de Ecotoxicologia e Biossegurança da Embrapa Meio Ambiente. Além disso, são tecidas considerações quanto ao significado da persistência do AMC no organismo animal.
Tipo: Folhetos Palavras-chave: Biopesticidas; Testes toxicológicos; Toxicologia; Análise de laboratório; Fungo para controle biológico; Cobaia; Análise de Risco; Biopesticides; Ecotoxicology; Animal tests.
Ano: 2012 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/936006
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Les polychlorobiphényles (PCB) en milieu marin : biogéochimie et écotoxicologie 5
Marchand, Michel; Abarnou, Alain; Marcaillou-le Baut, Claire.
This report synthesizes the knowledge on the behaviour, bioconcentration processes and ecotoxicity ofPCB in the marine environment. Contamination levels of the French coasts are examined. Concentration levels, mechanisms andfluxes between geochemicai reservoirs are assessed. Emphasis is given on subiethai effects and toxicity mechanisms. Quality standards used in European Community countries are listed. Conclusions and recommendations for environmentai management and research are given.
Tipo: Text Palavras-chave: PCB; Biogéochimie; Contamination; Écotoxicologie; PCBs; Biogeochemistry; Contamination; Ecotoxicology.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/00148/25954/24045.pdf
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The monitoring programme of the ecological and ecotoxicological consequences of the "Erika" oil spill 5
Laubier, Lucien; Le Moigne, Morgan; Flammarion, Patrick; Thybaud, Eric; Cossa, Daniel.
On December 11, 1999, Erika, an oil tanker flying the Maltese flag and loaded with 31 000 t of heavy fuel oil, was making its way from Dunkirk to Livorno when it encountered difficult weather conditions Southwest of the Brittany coast gale force winds 8 to 9 and 6 mwaves). On the morning of 12th December, the captain broadcast an SOS; his vessel was breaking in two, approximately 30 nautical miles to south of Penmarc'h Point (Fig. 1; Finistère, France). The fore section of the vessel sank during the night of the 12-13 December and the aft section was taken in tow on the morning December 13, but subsequently sank in the early afternoon. Both parts lay in 120 m of water and were located 10 km apart. Approximately, 19 000 t of heavy fuel oil were spilled into...
Tipo: Text Palavras-chave: Atlantique; France; Monitoring programme; Hydrocarbon; Erika; Marine pollution; Ecotoxicology; Ecology.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-1390.pdf
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Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure 5
Le Goic, Nelly; Hegaret, Helene; Boulais, Myrina; Beguel, Jean-philippe; Lambert, Christophe; Fabioux, Caroline; Soudant, Philippe.
The Pacific oyster Crassostrea gigas accounts for a large part of shellfish aquaculture production worldwide. Aspects of morphological and functional characteristics of oyster oocytes remain poorly documented, and traditional techniques, such as microscopic observations of shape or fertilization rate, are time and space consuming. The purpose of this study was to assess for the first time viability and reactive oxygen species (ROS) production of Pacific oyster oocytes using flow cytometry (FCM) and to apply this method to determine oocyte responses to in vitro exposure to the toxic dinoflagellate Alexandrium minutum. A culture of A. minutum caused a significant increase in oocyte ROS production, which gradually increased with the age of the culture, but...
Tipo: Text Palavras-chave: Pacific oyster; Gamete quality; ROS production; Viability; Flow cytometry; Ecotoxicology.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00216/32760/31321.pdf
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Utilisation des biomarqueurs pour la caractérisation de l’état écotoxicologique des masses d’eau 5
Sanchez, Wilfried; Porcher, J.-m..
In the past 25 years, numerous biomarkers have been developed with the objective to apply them to aquatic ecosystem biomonitoring. Recently, the Water Framework Directive (WFD) of the European union specified monitoring programs required to assess the achievement of good chemical and ecological status for all water bodies by 2015. This article reviews the potential of biomarkers for ecotoxicological status assessment in WFD monitoring programmes based on previous national and international experiments.
Tipo: Text Palavras-chave: Écotoxicologie; Biomarqueurs; Biosurveillance; Directive cadre sur l’eau; Ecotoxicology; Biomarkers; Biomonitoring; Water Framework Directive.
Ano: 2009 URL: https://archimer.ifremer.fr/doc/00500/61183/64703.pdf
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Mixtures of Chemical Pollutants at European Legislation Safety Concentrations: How Safe Are They? 5
Carvalho, Raquel N.; Arukwe, Augustine; Ait-aissa, Selim; Bado-nilles, Anne; Balzamo, Stefania; Baun, Anders; Belkin, Shimshon; Blaha, Ludek; Brion, Francois; Conti, Daniela; Creusot, Nicolas; Essig, Yona; Ferrero, Valentina E. V.; Flander-putrle, Vesna; Furhacker, Maria; Grillari-voglauer, Regina; Hogstrand, Christer; Jonas, Adam; Kharlyngdoh, Joubert B.; Loos, Robert; Lundebye, Anne-katrine; Modig, Carina; Olsson, Per-erik; Pillai, Smitha; Polak, Natasa; Potalivo, Monica; Sanchez, Wilfried; Schifferli, Andrea; Schirmer, Kristin; Sforzini, Susanna; Sturzenbaum, Stephen R.; Softeland, Liv; Turk, Valentina; Viarengo, Aldo; Werner, Inge; Yagur-kroll, Sharon; Zounkova, Radka; Lettieri, Teresa.
The risk posed by complex chemical mixtures in the environment to wildlife and humans is increasingly debated, but has been rarely tested under environmentally relevant scenarios. To address this issue, two mixtures of 14 or 19 substances of concern (pesticides, pharmaceuticals, heavy metals, polyaromatic hydrocarbons, a surfactant, and a plasticizer), each present at its safety limit concentration imposed by the European legislation, were prepared and tested for their toxic effects. The effects of the mixtures were assessed in 35 bioassays, based on 11 organisms representing different trophic levels. A consortium of 16 laboratories was involved in performing the bioassays. The mixtures elicited quantifiable toxic effects on some of the test systems...
Tipo: Text Palavras-chave: Bioassays; Effects; Mixtures; Ecotoxicology; Biomarkers.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00499/61115/64667.pdf
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Le mercure en milieu marin. Biogéochimie et écotoxicologie 5
Cossa, Daniel; Thibaud, Yves; Roméo, Michèle; Gnassia-barelli, Mauricette.
This report synthesizes the knowledge on the biogeochemical cycle, bioconcentration processes and ecotoxicity of mercury in the marine environment. Man induced changes on the mercury cycle, especially along the French coasts are reviewed. Concentration levels, mechanisms and fluxes between geochemical reservoirs are assessed. Emphasis is given on sublethal effects and toxicity mechanisms. Quality standards used in European Community countries are listed. Conclusions and recommendations for environmental management and research are given.
Tipo: Text Palavras-chave: Mercure; Biogéochimie; Contamination; Écotoxicologie; Mercury; Biogeochemistry; Contamination; Ecotoxicology.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/00332/44319/43890.pdf
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