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| Registros recuperados: 55 | |
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| SANTOS,Jaselita M.M. dos; TADEI,Wanderli Pedro; CONTEL,Eucléia P.B. |
| Of eleven proteins analyzed in four Amazonian populations, the esterases showed the greatest variation, with five activity zones. EST1, EST2 and EST5 showed variation in each of the populations studied. EST1 and EST2 are each controlled by two, and EST5 by four, codomi-nant alleles. LAP presented six activity zones, with codominant variation in LAP5and LAP6.oc—GPDH was monomorphic with one activity band on starch gel and two on polyacrylamide gel. 1DH presented two activity zones, with variation in the IDHl region. PGM had a single activity zone, with variation in all populations. The Ariquemes populations showed five alleles and the other populations three, all of then codominant. Three activity zones with two codominant alleles were observed for ODH.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anopheles darlingi; Isozymes; Electrophoresis. |
| Ano: 1996 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59671996000200097 |
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| Bespalhok, Danielle das Neves; Renesto, Erasmo; Signorini, Tiago. |
| Beetles of the species Sitophilus oryzae and S. zeamais are pests of great economic importance since they attack not only rice and maize but also several other cereals. In fact, these beetles are one of the most visible threats to sustainable food production. Current study estimated the genetic variability of S. oryzae in two samples, one from the State of Paraná (PR), Brazil, and another from the state of Rio Grande do Sul (RS), Brazil, and a sample of S. zeamais from the State of Santa Catarina (SC), Brazil. Isozyme electrophoresis in starch gel technique was employed to analyze eight enzyme systems (AAT, ACP, GDH, GPI, IDH, MDH, PGM and ME). Average heterozygosity rates were 0.0091, 0.0100 and 0.0000 and expected heterozygosity rates were 0.0419, 0.0452... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ciências Biológicas Genética. pest insect; Stored grains; Starch gel; Electrophoresis; Heterozygosity Genética.. |
| Ano: 2015 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/25143 |
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| Wendt,Simone Neumann; Sousa,Valderês Aparecida de; Sebbenn,Alexandre Magno; Sturion,José Alfredo; Luíz,Felipe; Santos,Elisa Caroline da Silva; Quoirin,Marguerite. |
| Eighteen enzyme systems were examined in Ilex paraguariensis St. Hil. using starch gel electrophoresis. Seven out of 12 active isozyme systems revealed one or more polymorphic loci (PGI, GOT, MR, G-6PDH, MDH, NDH, and 6-PGDH). However, the segregation and linkage analyses were performed only for PGI, GOT, G-6PDH and 6-PGDH systems. Gene segregation at these loci was regular, except for a few trees that showed segregation distortion. Weak linkage disequilibrium between loci was detected, but it was not enough to influence the multilocus estimate. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Erva-mate (matê); Electrophoresis; Loci; Allele; Segregation. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600016 |
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| Figueiredo,Vitória Ribeiro Garcia de; Justus,Ariana; Pereira,Dafne Garcia; Georgetti,Sandra Regina; Ida,Elza Iouko; Kurozawa,Louise Emy. |
| Abstract This work aimed to evaluate the enzymatic hydrolysis of okara protein concentrate with respect to degree of hydrolysis (DH) in order to obtain a protein hydrolysate with high antioxidant capacity and aglycones isoflavone content. A central composite rotatable design was carried out to evaluate the influence of temperature (40 to 70°C), enzyme:substrate ratio (0.5 to 5.0%, g/100g protein) and pH (7.0 to 9.0) on DH. The optimal condition was 55°C, pH 9 and enzyme:substrate ratio of 5.0%, resulting a DH value of 35.5%. After protein hydrolysis at optimal condition, the antioxidant capacities of hydrolysate increased from 58.29 to 383.49 μM Trolox equivalent/g solids (ABTS method) and 2.41 to 15.32 μM Trolox equivalent/g solids (FRAP method) when... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Soy pulp; Peptides; Cleavage of protein; Isoflavone profile; Electrophoresis; Experimental design. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100505 |
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| Santos-Filho,Sebastião David; Diniz,Claudia Leite; Carmo,Fernanda Santos do; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario. |
| The aim of this work was to study the influence of a walnut (Juglans regia) extract on the growth of Escherichia coli (E. coli) AB1157, on the plasmid DNA topology and on the labeling of blood constituents with technetium-99m (99mTc). An E. coli AB1157 culture, in stationary phase, was incubated with walnut and the growth of the culture was evaluated by optical density at 600 nm for 7 hours. Plasmid DNA samples were incubated with SnCl2 in presence or absence of walnut for 40 minutes, 0.8% agarose gel electrophoresis was performed, the gel was stained and the plasmid topological forms were visualized. Blood samples from Wistar rats were incubated with walnut extract and an assay of labeling of blood constituents with technetium-99m (99mTc) was performed.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; DNA; Electrophoresis; Stannous chloride; Technetium-99m; Juglans regia. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700026 |
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| Araújo,J.V.; Junghans,T.G.; Alfenas,A.C.; Gomes,A.P.S.. |
| Extraction and isoenzyme analysis of four isolates of Arthrobotrys including A. musiformis, A. robusta and A. conoides were conducted. Among the 14 enzymes studied by starch gel electrophoresis, using morpholine-citrate as gel/electrode buffer, the following nine enzymes showed interpretable banding patterns: <!-- $MVD$:face("Symbol") --><FONT FACE="Symbol">a</font>-esterase, fumarase, hexokinase, isocitrate dehydrogenase, leucine aminopeptidase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucomutase and phosphoglucoisomerase. All isolates studied displayed typical isoenzyme phenotypes for each species. Two isolates of A. conoides differed in their <!-- $MVD$:face("Symbol") --><FONT... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nematophagous fungi; Nematode-trapping fungus; Predacious fungi; Arthrobotrys; Isoenzymes; Electrophoresis. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000001 |
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| Marques,Thaís Lima; Von Pinho,Renzo Garcia; Von Pinho,Édila Vilela de Resende; Santos,Heloisa Oliveira dos. |
| ABSTRACT Knowing genes related to water deficit is a valuable tool to assist and accelerate the selection process of maize genotypes more adapted to drought conditions. Therefore, this work aimed to evaluate genes expression related to tolerance to water deficit in maize lines, using proteomic and transcriptomic analyses. Two lines previously classified as tolerant (91-T) and non-tolerant (57-NT) to low water availability were used. Dried seeds, ears tips, and seedlings (collected at three, five, and seven days after sowing) of the two maize lines (91-T and 57-NT) were used in proteomic analyses. Seedlings were subject to two contrasting water-availability conditions (70% and 10% of the water retention capacity). The expression of catalase, peroxidase, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zea mays; Abiotic stress; Electrophoresis; QRT-PCR.. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542019000100233 |
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| SCHMIDT,Michele Mantelli; FONTOURA,Andrine Menna da; VIDAL,Alessandra Roseline; DORNELLES,Rosa Cristina Prestes; KUBOTA,Ernesto Hashime; MELLO,Renius de Oliveira; CANSIAN,Rogério Luis; DEMIATE,Ivo Mottin; OLIVEIRA,Cristina Soltovski de. |
| Abstract Obtaining collagen and hydrolysates from mechanically separated chicken meat residue is an excellent way to add value to this waste. The aims of this study were to obtain collagen hydrolysates from chicken MSM residue using Alcalase® and Flavourzyme® enzymes, as well characterizing the functional, structural and thermal stability properties. The highest degree of hydrolysis was obtained using Alcalase®, 36.11%, while using Flavourzyme® resulted in 12.02%. The DSC analysis of the collagen indicated a denaturation temperature of 46.47 °C. The FTIR spectra of the crude collagen showed absorption peaks that were characteristic of amide bands A, B, I, II and III. In the spectra of the hydrolysed the area of the amide bands was reduced, and some peaks... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alcalase®; Electrophoresis; Flavourzyme®; FTIR; Functional properties. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020005006203 |
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| ZHU,Yan-Yun; ZHAO,Peng-Tao; WANG,Xiao-Yu; ZHANG,Juan; WANG,Xue- Hui; TIAN,Cheng-Rui; REN,Meng-Meng; CHEN,Tong-Guo; YUAN,Huan-Huan. |
| Abstract The potential astringency of eight grape cultivars was evaluated based on polyphenol/protein binding. The parameters such as weights, sizes, total phenols, flavanols and tannins of different grape tissues were determined, and the polyphenol/protein binding was analyzed by SDS-PAGE and spectroscopy. The Cabernet Sauvignon had the highest skin proportion, while the Blue French had the highest seed proportion. The Blue French seeds had the highest total phenolic content, while the Cabernet Sauvignon skins had the highest total phenolic content. SDS-PAGE showed that the seed extracts had a better affinity than skin extracts for saliva proteins, but fluorescence spectra demonstrated that the interactions of skin polyphenols with protein were more... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Grape cultivars; Polyphenols; Interaction; Electrophoresis; Spectroscopy. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000400930 |
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| Teodoro Pardo, Claudia Verónica de. |
| El objetivo de este estudio fue analizar la variabilidad genética en diferentes especies del género Anastrepha (Anastrepha ludens, A. obliqua, A. serpentina y A. striata), estudiar el flujo genético en A. ludens entre diferentes poblaciones de México y de Guatemala mediante un análisis isoenzimático, cuantificar ADN del ganglio cerebral y glándulas salivales y explorar la hibridación genómica in situ (GISH) en cada una de las especies de moscas en condiciones de laboratorio. Se analizaron los sistemas isoenzimáticos 6PGDH, IDH, ME, MDH, GPI y GOT en las cuatro especies de moscas en condiciones de laboratorio y campo, y para el flujo genético sólo cuatro sistemas fueron estudiados. Se examinaron 30 individuos (15 hembras y 15 machos) de cada población. Los... |
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Palavras-chave: Anastrepha; Contenido de ADN; Citometría de flujo; Electroforesis; GISH; Isoenzimas; Flujo genético; Variabilidad genética; DNA content; Flow cytometry; Electrophoresis; Isozymes; Gene flow; Genetic variability; Doctorado; Genética. |
| Ano: 2011 |
URL: http://hdl.handle.net/10521/569 |
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| Teodoro Pardo, Claudia Verónica de. |
| El objetivo de este estudio fue analizar la variabilidad genética en diferentes especies del género Anastrepha (Anastrepha ludens, A. obliqua, A. serpentina y A. striata), estudiar el flujo genético en A. ludens entre diferentes poblaciones de México y de Guatemala mediante un análisis isoenzimático, cuantificar ADN del ganglio cerebral y glándulas salivales y explorar la hibridación genómica in situ (GISH) en cada una de las especies de moscas en condiciones de laboratorio. Se analizaron los sistemas isoenzimáticos 6PGDH, IDH, ME, MDH, GPI y GOT en las cuatro especies de moscas en condiciones de laboratorio y campo, y para el flujo genético sólo cuatro sistemas fueron estudiados. Se examinaron 30 individuos (15 hembras y 15 machos) de cada población. Los... |
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Palavras-chave: Anastrepha; Contenido de ADN; Citometría de flujo; Electroforesis; GISH; Isoenzimas; Flujo genético; Variabilidad genética; DNA content; Flow cytometry; Electrophoresis; Isozymes; Gene flow; Genetic variability; Doctorado; Genética. |
| Ano: 2011 |
URL: http://hdl.handle.net/10521/569 |
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| Rocha,Vanessa Alves Lima; Maeda,Roberto Nobuyuki; Santa Anna,Lidia Maria Melo; Pereira,Nei. |
| This work aimed at the production of cellulases from pretreated sugarcane bagasse by the filamentous fungus Trichoderma harzianum IOC 3844 and their application in the hydrolysis of this same substrate, for a future use in second-generation ethanol production. The production of cellulases was optimized, which resulted in high enzymatic activities after 42 hrs of process in an instrumented bioreactor (CMCase 27,017 U x L-1; FPase 1,225 U x L-1; and β-glucosidase 609 U x L-1). The enzymatic extract was concentrated by using a hollow fiber membrane filtration system. The concentrated extract was applied in the hydrolysis of pretreated sugarcane bagasse, after 28 hrs of enzymatic reaction, displaying a similar catalytic performance of that attained... |
| Tipo: Journal article |
Palavras-chave: Electrophoresis; Enzymatic hydrolysis; Second generation ethanol; Trichoderma harzianum. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500012 |
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| Registros recuperados: 55 | |
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