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| Registros recuperados: 27 | |
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| Becker,Sidnei; Soares,Cíntia; Porto,Luismar Marques. |
| Groups of genes that produce exopolysaccharide with a N-acetyl-D-glucosamine monomer are in the genome of several pathogenic bacteria. Chromobacterium violaceum, an opportunistic pathogen, has the operon hmsHFR-CV2940, whose proteins can synthesize such polysaccharide. In this work, multiple alignments among proteins from bacteria that synthesize such polysaccharide were used to verify the existence of amino acids that might be critical for pathogen activity. Three-dimensional models were generated for spatial visualization of these amino acid residues. The analysis carried out showed that the protein HmsR preserves the amino acids D135, D228, Q264 and R267, considered critical for the formation of biofilms and, furthermore, that these amino acids are... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Exopolysaccharide; Chromobacterium violaceum pathogenicity; Comparative genomics. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300031 |
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| Velasco, C. Ruiz; Baud'Huin, M.; Sinquin, Corinne; Maillasson, M.; Heymann, D.; Colliec-jouault, Sylvia; Padrines, M.. |
| The growth and differentiation of bone cells is controlled by various factors which can be modulated by heparan sulphates. Here, we investigated the effects of an oversulphated exopolysaccharide (OS-EPS) on bone. We compared the effect of this compound with that of a native exopolysaccharide (EPS). Long-term administration of OS-EPS causes cancellous bone loss in mice due, in part, to an increase in the number of osteoclasts lining the trabecular bone surface. No significant difference in cancellous bone volume was found between EPS-treated mice and age-matched control mice, underlying the importance of sulphation in trabecular bone loss. However, the mechanism sustaining this osteoporosis was unclear. To clarify OS-EPS activities, we investigated the... |
| Tipo: Text |
Palavras-chave: Bone metabolism; Bone remodeling; Exopolysaccharide; Glycosaminoglycan; Heparin. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00032/14300/15749.pdf |
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| Zykwinska, Agata; Marquis, Melanie; Godin, Mathilde; Marchand, Laetitia; Sinquin, Corinne; Garnier, Catherine; Jonchere, Camille; Chedeville, Claire; Le Visage, Catherine; Guicheux, Jerome; Colliec-jouault, Sylvia; Cuenot, Stephane. |
| Articular cartilage is an avascular, non-innervated connective tissue with limited ability to regenerate. Articular degenerative processes arising from trauma, inflammation or due to aging are thus irreversible and may induce the loss of the joint function. To repair cartilaginous defects, tissue engineering approaches are under intense development. Association of cells and signalling proteins, such as growth factors, with biocompatible hydrogel matrix may lead to the regeneration of the healthy tissue. One current strategy to enhance both growth factor bioactivity and bioavailability is based on the delivery of these signalling proteins in microcarriers. In this context, the aim of the present study was to develop microcarriers by encapsulating... |
| Tipo: Text |
Palavras-chave: Exopolysaccharide; Growth factor; Microparticles; Microfluidics; Bioactivity. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00477/58882/61432.pdf |
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| Goudenege, David; Boursicot, Vincent; Versigny, Typhaine; Bonnetot, Sandrine; Ratiskol, Jacqueline; Sinquin, Corinne; Lapointe, Gisele; Le Roux, Frederique; Delbarre-ladrat, Christine. |
| Vibrio diabolicus, a marine bacterium originating from deep-sea hydrothermal vents, produces the HE800 exopolysaccharide with high value for biotechnological purposes, especially for human health. Its genome was sequenced and analyzed; phylogenetic analysis using the core genome revealed V. diabolicus is close to another deep-sea Vibrio sp. (Ex25) within the Harveyi clade and Alginolyticus group. A genetic locus homologous to the syp cluster from Vibrio fischeri was demonstrated to be involved in the HE800 production. However, few genetic particularities suggest that the regulation of syp expression may be different in V. diabolicus. The presence of several types of glycosyltransferases within the locus indicates a capacity to generate diversity in the... |
| Tipo: Text |
Palavras-chave: Genome; Vibrio; Exopolysaccharide; Biosynthesis. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00241/35244/33892.pdf |
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| Toullec, Jordan; Moriceau, Brivaela. |
| Diatom production is mainly supported by the dissolution of biogenic silica (bSiO(2)) within the first 200 m of the water column. The upper oceanic layer is enriched in dissolved and/or colloidal organic matter, such as exopolymeric polysaccharides (EPS) and transparent exopolymeric particles (TEP) excreted by phytoplankton in large amounts, especially at the end of a bloom. In this study we explored for the first time the direct influence of TEP-enriched diatom excretions on bSiO(2) dissolution. Twelve dissolution experiments on fresh and fossil diatom frustules were carried out on seawater containing different concentrations of TEP extracted from diatom cultures. Fresh diatom frustules were cleaned from the organic matter by low ash temperature, and... |
| Tipo: Text |
Palavras-chave: Exopolysaccharide; Diatom excretion; Diatomite; Chaetoceros muelleri; Biogenic silica; BSiO(2) dissolution; TEP. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00616/72795/72270.pdf |
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| Chalkiadakis, Eletherios; Dufourcq, R.; Schmitt, Sophie; Brandily, Christophe; Kervarec, N.; Coatanea, Denis; Amir, H.; Loubersac, Lionel; Chanteau, S.; Guezennec, Jean; Dupont-rouzeyrol, M.; Simon-colin, Christelle. |
| Aims Exopolysaccharides (EPS) are industrially valuable molecules with numerous useful properties. This study describes the techniques used for the identification of a novel Vibrio bacterium and preliminary characterization of its EPS. Methods and Results Bioprospection in marine intertidal areas of New Caledonia followed by screening for EPS producing brought to selection of the isolate NC470. Phylogenetic analysis (biochemical tests, gene sequencing and DNADNA relatedness) permitted to identify NC470 as a new member of the Vibrio genus. The EPS was produced in batch fermentation, purified using the ultrafiltration process and analysed by colorimetry, Fourier Transform Infrared spectroscopy, gas chromatography, Nuclear Magnetic Resonance and HPLC-size... |
| Tipo: Text |
Palavras-chave: Biopolymers; Biotechnology; Exopolysaccharide; Fermentation biotechnology. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00144/25501/24036.pdf |
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| Zykwinska, Agata; Berre, Ludivine Tripon-le; Sinquin, Corinne; Ropartz, David; Rogniaux, Helene; Colliec-jouault, Sylvia; Delbarre-ladrat, Christine. |
| Polysaccharides have attracted much attention due to their interesting physico-chemical and also biological properties that are explored in food, cosmetic and pharmaceutical industries. GY785 exopolysaccharide (EPS) presenting an unusual structure is secreted by the deep-sea hydrothermal bacterium, Alteromonas infernus. Low-molecular weight (LMW) derivatives obtained by chemical depolymerization of the native high molecular weight (HMW) EPS were previously shown to exhibit biological properties similar to glycosaminoglycans (GAG). In the present study, in order to generate well defined derivatives with a better control of the depolymerization, an enzymatic approach was applied for the first time. Various commercially available enzymes were firstly screened... |
| Tipo: Text |
Palavras-chave: Exopolysaccharide; Enzyme; Depolymerization; Oligosaccharide; Mass spectrometry. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00423/53458/54352.pdf |
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| Simon-colin, Christelle; Gueguen, Yannick; Bachere, Evelyne; Kouzayha, Achraf; Saulnier, Denis; Gayet, Nicolas; Guezennec, Jean. |
| Cultured pearls are the product of grafting and rearing of Pinctada margaritifera pearl oysters in their natural environment. Nucleus rejections and oyster mortality appear to result from bacterial infections or from an inappropriate grafting practice. To reduce the impact of bacterial infections, synthetic antibiotics have been applied during the grafting practice. However, the use of such antibiotics presents a number of problems associated with their incomplete biodegradability, limited efficacy in some cases, and an increased risk of selecting for antimicrobial resistant bacteria. We investigated the application of a marine antimicrobial peptide, tachyplesin, which is present in the Japanese horseshoe crab Tachypleus tridentatus, in combination with... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Exopolysaccharide; Tachyplesin; Antimicrobial. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00275/38599/37128.pdf |
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| Colliec-jouault, Sylvia; Zanchetta, P; Helley, D; Ratiskol, Jacqueline; Sinquin, Corinne; Fischer, A. M.; Guezennec, Jean. |
| Bacterial polysaccharides offer fascinating potential applications for the pharmaceutical industry. Although many known marine bacteria produce exopolysaccharides (EPS), continuation in looking for new polysaccharide-producing micro-organisms is promising. Marine bacteria, isolated from deep-sea hydrothermal vents, have demonstrated their ability to produce in aerobic conditions, unusual EPS. With the aim of discovering biological activities, EPS presenting different structural features were studied. An EPS secreted by Vibrio, diabolicus was evaluated on the restoration of bone integrity in experimental model and was demonstrated to be a strong bone-healing material. Another EPS produced by Alteromonas infernus was modified in order to obtain new... |
| Tipo: Text |
Palavras-chave: Hydrothermal vents; Exopolysaccharides; Bone healing; Biomimetic; Anticoagulant activity; Sources hydrothermales; Exopolysaccharide; Cicatrisation osseuse; Biomimétique; Activité anticoagulante. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-2117.pdf |
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| Leo, Fiame; Hashida, Shukichi; Kumagai, Daiju; Uchida, Kenji; Motoshima, Hidemasa; Arai, Ikichi; Asakuma, Sadaki; Fukuda, Kenji; Urashima, Tadasu; 福田, 健二; 浦島, 匡. |
| A neutral exopolysaccharide produced in large amounts by Lactobacillus fermentum TDS030603 was purified from culture supernatants and its structure determined by monosaccharide analysis, methylation analysis and 1H-NMR spectroscopy. Optimum conditions for the production of this exopolysaccharide by the above bacterial strain, when cultured using cheese whey medium or MRS broth, were also investigated. The exopolysaccharide was found to be composed of D-galactose and D-glucose in the molar ratio of 1:2.5 and to consist of one major as well as one minor saccharide, both of which contained a tetrasaccharide repeating unit consisting of two α and two β anomers. The major saccharide contained non-reducing glucopyranose, O-3 substituted glucopyranose, O-6... |
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Palavras-chave: Lactic acid bacteria; Exopolysaccharide; Lactobacillus fermentum TDS030603. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4183 |
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| Fukuda, Kenji; Shi, Tala; Nagami, Kentaro; Leo, Fiame; Nakamura, Tadashi; Yasuda, Kumi; Senda, Akitsugu; Motoshima, Hidemasa; Urashima, Tadasu; 福田, 健二; 浦島, 匡. |
| A thermophilic lactic acid bacterium, Lactobacillus fermentum TDS030603, produced about 100 mg/L of EPS in purified form when grew in de Man-Rogosa-Sharpe (MRS) broth. The 1% (w/v) solution of the purified EPS was highly viscous, exhibiting an apparent 22 viscosity (ηapp) of 0.88 Pa s at a shear rate of 10/s. To investigate the impact of carbohydrate source on the production yield and chemical structure of EPS and the viscosity of EPS solution, a chemically defined medium (CDM) has been developed. Results of TLC, HPLC, 25 and 1H NMR spectroscopy indicated that the chemical structures of EPS released in MRS and in the CDM supplemented with glucose, galactose, lactose or sucrose were very similar. All the 1% solutions of EPSs released in CDMs were highly... |
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Palavras-chave: Chemical structure; Chemically defined medium; Exopolysaccharide; Lactic acid bacteria; Viscosity. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2694 |
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| XU,CHUNPING; YU,JINWEI; ZHAO,SHANSHAN; WU,SHUNGSHUNG; HE,PEIXIN; JIA,XUEWEI; LIU,YANQI; MAO,DUOBIN. |
| ABSTRACT The effect on different three carbon source (i.e. glucose, fructose and sucrose) on production, chemical characterization and antioxidant activity of exopolysaccharide (EPS) produced by Phellinus vaninii Ljup was investigated in this study. Amongst carbon sources examined, glucose and sucrose were favorable for the mycelia growth, while the maximum EPS yield was achieved when sucrose was employed. The predominant carbohydrate compositions in EPSs identified were gluconic acid, glucose, mannose and galactose acid. Then, FT-IR spectral analysis revealed prominent characteristic groups in EPSs. EPSs molecule exist as nearly globular shape form in aqueous solution. The variation also affects antioxidant activities by investigated by using hydroxyl and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phellinus vaninii Ljup; Exopolysaccharide; Carbon source; Antioxidant activity; Composition. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502033 |
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| Paulo,Elinalva M.; Boffo,Elisangela F.; Branco,Alexsandro; Valente,Ângela M.M.P.; Melo,Itamar S.; Ferreira,Antonio G.; Roque,Milton R.A.; Assis,Sandra A. de. |
| The genus Leuconostoc belongs to a group of lactic acid bacteria usually isolated from fermented vegetables, which includes species involved in the production of exopolysaccharides (EPS). These biopolymers possess considerable commercial potential. Because of the wide variety of industrial applications of EPS, this study aimed to produce and characterize the native exopolysaccharide strain Leuconostoc pseudomesenteroides R2, which was isolated from cabbage collected in a semi-arid region of Bahia. We employed the following conditions for the production of EPS: 10.7% sucrose, pH 8.2, without agitation and incubation at 28ºC for 30 hours. The fermentation broth was treated with ethanol and generated two types of polysaccharide substances (EPS I and EPS II).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leuconostoc pseudomesenteroides; Exopolysaccharide; Polymer; Dextran. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200018 |
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| Registros recuperados: 27 | |
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